Study antibacterial effect of leaves of Cleistocalyx operculatus on micropipet slap of Vanden Bergher an Vilietlink (1994), testing effect of useful for gall in white house-mouse, testing ability of make toxic for cell follow America of National cancer study Institute’s method. Result: leaves of cleistocalyx operculatus, special kept leaves have good effect on E. coli bacterium and 2 Gr(+) bacteria. Decoction of kept leaves is very useful for gall. Both of essential oil and fully dry jelly of 4 test samples extract from leaves of cleistocalyx operculatus (TDV, TDVU, CKV, CKVU) also inhibit development of cancer cells.
Biological Markers ; Biological Products ; Anti-Bacterial Agents ; Plants, Medicinal

Tam Hoang powder (constituents: rhizoma Coptidis, cortex Oroxyli, radix and caulis Fibraurea, CuSO4) was used for healing process after hemorrhoid treatment. Chemical analysis showed that the prescription contained alkaloids (two of them were berberine and palmatine), flavonoids, amino acids, tannin, sterols and reduced sugars. The powder fluid extract exhibited antimicrobial activities against 5 Gr (-) and 5 Gr (+) bacteria. Tam Hoang powder proved to have good healing effect on over 85% of 500 hemorrhoid patients.
Medicine, Traditional ; Hemorrhoids ; Chemistry ; Therapeutics

Objective: To examine the in vitro and in vivo anti-inflammatory effects of the alkaloid enriched extract (ELA) from the roots of Eurycoma longifolia. Methods: The in vitro antiinflammatory effects of ELA were evaluated by examining its inhibitory activities against nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) and cyclooxygenase 2 (COX-2) expressions in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The level of NO produced in the culture media was determined by Griess method. The iNOS and COX-2 protein expressions were analyzed by Western blot. The in vivo effect of ELA was evaluated on LPS-induced septic shock in mice model. Mice mortality was monitored for 5 days after injection of LPS. The chemical contents of the ELA were determined by using various chromatographic and spectroscopic techniques. Results: The ELA was found to exhibit a significant anti-inflammatory effect in both in vitro and in vivo models. The results demonstrated that ELA dose-dependently inhibited LPS-induced NO production as well as the protein iNOS and COX-2 expressions. In the septic shock model, ELA dose-dependently protected mice from LPS-induced mortality. Further study on the isolated components of ELA indicated that 9,10-dimethoxycanthin-6-one may contribute significantly to the anti-inflammatory effects of the extract. Conclusions: These results suggest that ELA exhibits the anti-inflammatory activity via suppression of pro-inflammatory mediators such as NO, iNOS, and COX-2 and protects mice from LPS-induced mortality in septic shock model.