By studying on extraction technology of desertliving cistanche polysaccharides, it provided evidences for development and utilization of cistanche. Anthrone-sulfuric acid method was used in the measurement of desertliving cistanche polysaccharides. The yield of desertliving cistanche polysaccharides was used as index. Different types of extraction methods were compared. Then, the extraction process was optimized by orthogonal experiment. The results showed that the best extraction process was Tween-60 ultrasonic cooperated. The best extraction process was when the mass concentration of Tween-60 was 0.5%, solid-to-liquid ratio was 1:25, temperature was 60℃, and the extraction time was 30 min. The extraction yield of desertliving cistanche polysaccharides was 8.17%. It was concluded that this extraction technology was reasonable and reliable, which can be used in the development and utilization of cistanche.