[Objective] To summarize professor WEN Chengping's scholar thoughts and experience of both the syndrome differentiation and treatment of psoriasis. [Methods] From causa morbi, mechanism and clinical treatment based on differentiation of signs, it expounds Professor WEN 's clinical experience and academic points, exampled with a common medical case.[Results] Professor WEN Chengping believed that blood toxin,stagnation,and blood stasisis the key pathogenesis of psoriasis. He divides the psoriasis into types of blood-heat, blood dryness, yang stagnation. And it points out that we should need to pay attention to invigorating the circulation of blood and strengthening the spleen and stomach.[Conclusions] Professor WEN Chengping is specialized in getting rid of the old thoughts by developing a new school of his own. And a set of characteristic syndrome differentiation used by WEN blazes a new trail for the treatment.

AIM: To study the EBV LMP-1 gene integrated in the chromosome of poorly differentiated nasopharyngeal carcinoma cell line SUNE-1. METHODS: The LMP-1 gene of SUNE-1 was detected with PCR; Deletion of LMP-1 was examined by restriction endonuclease analysis and PCR. The deletion was precisely localized by DNA sequencing. RESULTS: The LMP-1 gene integrated in the chromosome of SUNE-1 could be deleted or non-deleted. The two introns of LMP-1 gene were shown being lost in SUNE-1 cell line. CONCLUSION: Deletion of intron 1 and intron 2 happen in some of the LMP-1 gene integrated in the chromosome of SUNE-1.

Objective:To establish a method of multi-PCR to amplify the complete DNA sequence (CDS) of TCR ? and ? chain of the antigen-specific T lymphocytes in local pathologic specimen of active pulmonary tuberculosis patients, and to analyze ?/? T cell receptor gene rearrangement and CDR3 repertoire.Methods:The lymphocytes in bronchoalveolar lavage (BAL) of active pulmonary tuberculosis patients were separated. Following total RNA extraction, cDNA synthesis, Multi-PCR, recombinant clones construction, and sequencing, the CDS of TCR ? and ? chains from these lymphocytes were analyzed by using software of DNAstar and internet TCR resources.Results:24 of ? chain CDS and 13 of ? chain CDS from 3 samples of BAL were obtained. As for TCR ? chain, AV1S2 (54%), AV12S3 (41%), and AV12S2(5%) appeared frequently. BV2(38%), BV29S1(46%), BV14(3%), and BV4S2(3%) in TCR ? chain appeared more often. There were CDR3 diversities between samples and even in the same sample by amino acid sequence analysis, but there were a few identical or similar amino acid sequences. There was the same amino acid sequence of SVGTGTLHQETQY in CDR3 region of ? chain of BAL sample No.1 and No.2; The sequence of AVRDWAGNMLT appeared in two ? chains of BAL sample No.2 and No.3; Moreover, the sequence of AV…DNN…RLM appeared in ? chains of BAL sample No.2 and No.3.Conclusion:A method of Multi-PCR is used to amplify TCR ? and ? chain CDS of tuberculosis patients. There are characteristic T cell clones to proliferate,with TCR ? and ? chain repertiore skewing in local infective focus. The sequences of CDR3 in different TCR clones are mostly different but there are a few identical or similar sequences in the same patient or even between different patients. The identical amino acid sequences of CDR3 are possibly specific for recognizing MTB polypeptide.

Since the COVID-19 outbreak, medical institutions in different parts of the country have experienced personal protective equipment(PPE) shortages at different levels. The situation is especially severe in the COVID-19 epicenter. Overseas donation is one of the effective ways to ease the shortage of PPE. Based on the practice of Union Hospital of Tongji Medical College of Huazhong University of Science and Technology, the authors discussed the difficulties and risks for public hospitals to receive overseas donations, summarized experience and proposed suggestions which can be used as reference for other public hospitals and related departments.

Since the COVID-19 epidemic broke out in Wuhan, medical institutions in different parts the country have experienced personal protective equipment (PPE) shortages at different levels. The situation is especially severe in the coronavirus epicenter. Overseas donation is one of the effective methods to ease the shortage of protective equipment. Based on the practice of Union Hospital of Tongji Medical College of Huazhong University of Science and Technology, the article discussed the difficulties and risks for public hospitals to receive overseas donations, summarized experience and proposed suggestions which can be used as reference for other public hospitals and related departments.