1.Study on Biosensor for Ag+and Cysteine Quantification Based on Magnetic Nanoparticles and Intermolecular Split G-Quardruplex-Hemin DNAzymes
Xun FU ; Danyu GU ; Shengdong ZHAO ; Shutong WEN ; He ZHANG
Chinese Journal of Analytical Chemistry 2016;44(10):1487-1494
Based on untermolecular splut G-quardruplex-hemun DNAzymes, a buosensor for detectuon of sulver uons and cysteune was developed wuth magnetuc nanopartucles ( MNPs ) as carruer to ummobuluze the DNA probes. Sunce Ag+ chelates guanune bases at the bundung sutes whuch are unvolved un G-quadruplex formatuon, the presence of Ag+ may unhubut G-quartets connected by Hoogsteen-type base paurung and dusrupt G-quadruplexes structures, whuch decreases the peroxudase actuvuty of G-quadruplex-hemun DNAzymes that effucuently catalyze H2 O2-meduated reactuons, such as the oxudatuon of ABTS ( 2, 2'-azunobus ( 3-ethylbenzothuozolune)-6-sulfonuc acud) by H2 O2 . Moreover, un the presence of L-cysteune, ut was used as a competutor by the strongly Ag-S to release Ag+ from G-ruch olugonucleotudes, promotung the reformatuon of G-quadruplexes and uncreasung the peroxudase actuvuty, whuch catalyzes the ABTS-H2 O2 reactuon system. In thus experument, the effucuent separatuon from real sample was achueved usung magnetuc nanopartucles as a solud phase carruer to effectuvely uncrease the detectuon sensutuvuty and decrease the background sugnal. Under the optumum condutuons, a hugh lunear relatuonshup between the UV absorbance and the Ag+ concentratuon was establushed un the range of 0. 5-100 nmol/L wuth a detectuon lumut of 0. 2 nmol/L. The calubratuon curve of cysteune was udentufued un the range from 0. 1 to 80 nmol/L and the detectuon lumut was as low as 0. 04 nmol/L.
2.Research progress of application of traditional Chinese medicine nursing in cervical spondylosis
Lei CHEN ; Hua CHEN ; Ying DA ; Leilei ZHU ; Danyu ZHANG
Chinese Journal of Practical Nursing 2016;32(27):2157-2160
The effects of Traditional Chinese medicine (TCM) nursing operation in cervical spondylitis have been generally recognized. This paper reviewed the applications and improvements of the major three TCM nursing operations, cupping, Gua-Sha and acupressure. The future developments in moxibustion, auricular point sticking, fumigation and washing therapy and moisten compress therapy were also prospected.
3.Erythropoietin inhibits complement 3a-induced renal tubular epithelial to mesenchymal transition
Fengxia ZHANG ; Jianxin WAN ; Binbin FU ; Jiong CUI ; Yanfang XU ; Danyu YOU
Chinese Journal of Nephrology 2012;28(2):115-120
Objective To investigate the effects of erythropoietin (EPO) on complement 3a (C3a)-induced renal tubular epithelial to mesenchymal transition. Methods The HK-2 cells were divided into 6 groups namely control group,EPO group,TGF-β group,TGF-β+EPO group,C3a group and EPO+C3a group.The mRNA and protein expressions of α-SMA,E-cadherin and C3 were investigated by RT-PCR,Western blot and immunofluorescence respectively. Results Compared with control group and EPO group,the mRNA and protein expressions of α-SMA in HK-2 cells were up-regulated after the intervention of C3a or TGF-β (all P<0.05).On the contrast,the mRNA and protein expressions of E-cadherin were down-regulated(P<0.05),the mRNA and protein expressions of C3 were enhanced (all P<0.05).However,all those above effects of C3a or TGF-β were inhibited after the intervention of EPO (all P<0.05). Conclusion EPO is capable of suppressing the epithelial to mesenchymal transition induced by C3a.
4.EFFECTS OF HCY ON THE EXPRESSION OF HCY-2 AND THE PROLIFERATION IN HUMAN FETUS ARTERIAL SMOOTH MUSCLE CELLS
Danyu LU ; Guangming WANG ; Xiao HAN ; Lina ZHANG ; Jun WU ; Guanghui CHEN ; Ying LI
Acta Anatomica Sinica 1957;0(04):-
Objective To study the homocysteine induced gene(HCY-2) expression in human fetus arterial smooth muscle cells(hASMCs) in culture and explore the effects of homocysteine(HCY) and folic acid on HCY-2 expression and cell proliferation of hASMCs. Methods Immunohistochemistry ABC staining method was used to observe and analyze HCY-2 expression in hASMCs in culture.The image analysis system was used to research of hASMCs quantificationally.The effect of different HCY concentration on the proliferation of hASMCs was investigated by the cell counting. Results Immunoreactive substance of HCY-2 was chiefly found in cytoplasm of hASMCs.The expression of HCY-2 could be affected by HCY concentrations.There was a positive dose-dependent correlation with HCY concentrations in the culture medium.Folic acid increased the expression of HCY-2.The different concentration of HCY enhanced the proliferation of hASMCs,and this enhancement was maximal at the concentration of 1.25 mmol/L of HCY,while the proliferation was decreased when the concentration of HCY was over 1.25 mmol/L.Conclusion HCY increases the expression of HCY-2,and affects the proliferation of hASMCs.HCY is inhibited by folic acid.
5.Correlation between event-related potential P300 and violent behavior in patients with schizophrenia
Danyu LI ; Chaomeng LIU ; Meizi WANG ; Lan XIAO ; Guiqing ZHANG
Chinese Journal of Behavioral Medicine and Brain Science 2021;30(8):722-727
Objective:To explore the change characteristics of event-related potential P300 in different violence risk levels of schizophrenic patients and analyze the risk factors of violence in schizophrenic patients.Methods:Totally 158 schizophrenic patients in Lyuzhou hospital of Shihezi City from January 2019 to August 2020 were collected and assessed with the violence risk scale for 3 days.According to the assessment results, the patients who met the inclusion criteria were divided into low-risk group( n=78), medium-risk group( n=51) and high-risk group( n=29). The auditory P300 of patients in each group was completed within 3 days and act of violence was observed and recorded within one week.Data analysis was carried out by SPSS 20.0 software.The changes of P300 in different violence risk groups were analyzed by ANOVA, and the influencing factors of violence in patients with schizophrenia were analyzed by logistic regression. Results:There was no significant difference in latency of P300 among the three groups (χ 2=4.71, P=0.10), but there was significant difference in amplitude of P300( F=6.67, P<0.01). Compared with the low-risk group ((12.14±9.19) μV), the amplitude of P300 in medium-risk group ((8.25±7.13) μV) and high risk group ((6.71±4.97) μV) decreased significantly ( t=-3.14, -5.45, both P<0.05). There was no significant difference in amplitude of P300 between the high-risk group and the middle-risk group( t=-2.31, P>0.05). The latency and amplitude of schizophrenia patients with violent behavior were significantly different from those without violent behavior ( Z=-6.30, 9.78, both P<0.01). High BVC grade (compared with high-risk group, low-risk group: OR=0.03, 95% CI : 0.00-0.35; the middle risk group: OR=0.09, 95% CI : 0.01-0.62), prolonged P300 latency ( OR=1.30, 95% CI : 1.13-1.48) and decreased P300 amplitude ( OR=0.50, 95% CI: 0.36-0.70), delusion of victimization ( OR=0.12, 95% CI: 0.02-0.76)were the risk factors for violent behavior. Conclusion:The latency and amplitude of P300 can be used as the reliable neuroelectrophysiological indicators for evaluating violence risk in patients with schizophrenia.It has important clinical application value for evaluating violence in patients with schizophrenia.
6.Simultaneous determination of metolazone and valsartan in beagle dog plasma using liquid chromatography-tandem mass spectrometry
Meiling CHEN ; Jiezhao ZHOU ; Xiaohui CHENG ; Zhiping LI ; Rutao WANG ; Wanzhi ZHANG ; Danyu MEI ; Xiuhua WANG ; Ying LI ; Xingguo MEI
Military Medical Sciences 2016;40(4):346-349
Objective To develop an LC-MS/MS method for simultaneous determination of metolazone and valsartan in beagle dog plasma.Methods The chromatographic separation was achieved on an Agilent Poroshell 120(2.1 mm ×30 mm × 2.7 μm)analytical column.The multiple reaction monitoring mode operating in positive ion was adopted in MS detection, and precursors to the product ion transitions of m/z 366.2/259, 436.2/291 and 423.4/207 were used to measure metola-zone, valsartan and internal standard ( losartan potassium) .Results The method was linear over the concentration range of 0.5 ng/ml-100 ng/ml for metolazone and 5-5000 ng/ml for valsartan, with the correlation coefficients ( r2 ) of 0.9937 and 0.9939, respectively.The average intra-day precision values ( RSD) were 2.09% -8.85% for metolazone and 2.36%-13.12%for valsartan.The matrix effect values were 87.73%-98.62%for metolazone and 99.03%-137.35%for valsartan.The average recovery was 75.74%-81.82%for metolazone and 83.89%-95.64%for valsartan.Conclu-sion This analytical method for metolazone and valsartan is simple, accurate and sensitive, so it can be used for pharma-cokinetic research of metolazone and valsartan immediate release tablets in beagle dogs.
7.Dietary fiber intake and its correlation with cardiac event-free survival in patients with heart failure
Chenglin ZHANG ; Xiaohua WANG ; Nan ZHANG ; Danyu HOU ; Qin SHEN
Chinese Journal of Clinical Nutrition 2021;29(1):35-41
Objective:To investigate the daily dietary fiber intake, the main adverse cardiovascular events during follow-up and the cardiac event-free survival in patients with chronic heart failure, and to analyze the relationship between dietary fiber intake and cardiac event-free survival in this population.Methods:This study was a prospective investigation. The investigation was performed in chronic heart failure patients from three third-class hospitals in Suzhou using general information questionnaire and 3-day diet diary. Major adverse cardiovascular events within 6 months after discharge and event-free survival were obtained by telephone call and medical records.Results:The incidence of major cardiovascular adverse events in 122 patients with heart failure was 27.9% within 6 months after discharge. Their dietary fiber intake was 8.1(5.8-10.9)g/d, lower than the recommended intake. Kaplan-Meier plots and log-rank tests demonstrated that cardiac event-free survival was significantly shorter in patients with dietary fiber deficiency ( P=0.043). Patients with dietary fiber intake ≥6 g/d after discharge showed decreased risk of major adverse cardiovascular events compared to patients with dietary fiber intake<6 g/d ( HR=0.422; 95% CI=0.189-0.942; P=0.035). Conclusions:This study revealed insufficient dietary fiber intake in patients with heart failure. Insufficient dietary fiber intake was associated with higher risk of major adverse cardiovascular events and shorter cardiac event-free survival in heart failure patients within 6 months after discharge. In conclusion, patients with heart failure can increase dietary fiber intake in order to improve prognosis.
8.Prospect on medical treatment of pulmonary mucinous adenocarcinoma
Danyu LIU ; Yuhong GONG ; Xiang ZHAO ; Meng ZHANG ; Qi ZHANG ; Cuiyan GUO ; Ligong NIE ; Yuan CHENG
Clinical Medicine of China 2023;39(4):241-245
Pulmonary mucinous adenocarcinoma is a subtype of lung adenocarcinoma, among which invasive mucinous adenocarcinoma (IMA) is the most common subtype and is easily misdiagnosed as pneumonia. Its etiology and pathogenesis are unclear and may be related to gene mutations and other factors. Due to its relative rarity and few related studies, guidelines do not provide advices on its treatment. KRAS mutations are common in IMA patients, and Sotorasib may be effective against KRAS G12C mutated IMA. NRG1 fusion is considered to be an important driver of IMA, and afatinib may be effective in treating IMA with NRG1 fusion/rearrangement. PD-L1 expression is very low in IMA patients, while B7-H3 expression is high, so B7-H3 may be a potential immunotherapeutic target.
9.Association of time in range with the severity of coronary artery disease in type 2 diabetic patients
Danyu WANG ; Kaikun LIU ; Yun ZHANG ; Junpeng YANG ; Hongyan HENG ; Xinru DENG ; Xueli YANG ; Huijuan YUAN
Chinese Journal of Endocrinology and Metabolism 2021;37(11):973-978
Objective:To investigate the association of time in range(TIR) with the severity of coronary artery disease and acute coronary syndrome in patients with type 2 diabetes mellitus.Methods:A total of 216 patients with type 2 diabetes mellitus and coronary heart disease were recruited and undergone anthropometric and biochemical measurements, continuous glucose monitoring, and calculation of SYNTAX score. TIR was defined as the percentage of time within the glucose range of 3.9-10.0 mmol/L during 24 h. Spearman correlation analysis and multivariate linear regression analysis were used to evaluate the correlation factors of SYNTAX score. Multivariate logistic regression analysis was used to analyze the association of TIR with the severity of coronary artery disease and acute coronary syndrome. Results:Compared with patients with mild coronary artery disease, TIR in patients with moderate to severe coronary artery disease was lower[(69.4±17.3)% vs (60.8±17.8)%, t=3.0, P=0.003], and HbA 1C of patients with moderate to severe coronary artery disease was higher [(9.6±1.7)% vs (8.8±1.6)%, t=3.3, P=0.001]. SYNTAX score was negatively correlated with TIR ( r=-0.251, P<0.01) and positively correlated with HbA 1C ( r=0.249, P<0.01). Moreover, compared with HbA 1C (standardized coefficients=0.181, P=0.007), TIR (standardized coefficients=-0.192, P=0.004) had a greater influence on SYNTAX score. Multivariate linear regression analysis showed that TIR, HbA 1C, duration of diabetes and smoking were independently correlated with SYNTAX score. Multivariate logistic regression analysis revealed that compared with TIR Q1, Q3 and Q4 were independent protective factors for moderate to severe coronary artery disease (respectively, OR=0.61 and 0.59, 95% CI 0.39-0.96 and 0.38-0.94, P=0.014 and 0.009) and acute coronary syndrome (respectively, OR=0.51 and 0.39, 95% CI 0.32-0.95 and 0.26-0.75, P=0.022 and 0.008). Conclusion:TIR was significantly and independently correlated with the severity of coronary artery disease and acute coronary syndrome in type 2 diabetes mellitus after controlling confounding factors. When TIR level was decreased, the severity of coronary artery disease was aggravated, and SYNTAX score and the risk of acute coronary syndrome was increased.
10.Autologous platelet gel-collagen biologically active composite membrane for repair of periodontal bone defect in rats
Xufeng ZHANG ; Qiya FU ; Genjian ZHENG ; Yusu GUO ; Danyu CHEN ; Fangman FU ; Hui WU ; Lin WANG
Chinese Journal of Tissue Engineering Research 2019;23(14):2177-2182
BACKGROUND: In recent years, the development of tissue engineering has provided a new approach for the treatment of periodontal bone defect. Tissue engineering therapy includes seed cells, scaffolds and growth factors. Platelet gel contains a large number of platelet growth factors, and collagen is often used for the preparation of scaffold materials. Therefore, the platelet gel and collagen biologically active composite membrane can provide scaffolds and growth factors for the defect bone. OBJECTIVE: To investigate the effect of autologous platelet gel-collagen biologically active composite membrane on the repair of periodontal bone defect in rats. METHODS: Forty-two Wistar rats (Shanghai Xipuer-Bikai Experimental Animal Co., Ltd., China) were selected. (1) Collagen was cut into 5 mm×2 mm size, and 10 mL of whole blood was extracted from 6 rats to obtain platelet-rich plasma. Autologous platelet gel-collagen composite membrane was prepared by adding bovine thrombin, calcium chloride and collagen in a certain proportion. Platelets in whole blood and in platelet-rich plasma were detected. The levels of platelet derived growth factor AB, transforming growth factor-β, basic fibroblast growth factor and vascular endothelial growth factor in whole blood and platelet-rich plasma were detected by ELISA. (2) The models of mandibular periosteal defect were established in 36 rats (the size of the bone defect was 5 mm×2 mm, and the root surface cementum was removed) , and randomly divided into two groups. Autologous platelet gel-collagen group placed the autologous platelet gel-collagen composite membrane in the bone defect, and the control group did not place any materials. The hematoxylin-eosin staining of periodontal tissues of rats in each group was analyzed at 2, 4 and 8 weeks after surgery. Rate of new born, new centumum formation, new alveolar bone formation, and new periodontal ligament tissue formation height were measured. The expression of bone morphogenetic protein-2 was detected by immunohistochemical staining. RESULTS AND CONCLUSION: (1) The mean platelet count in platelet-rich plasma was 4.78 times as high as the whole blood, indicating that the number of platelets increased significantly after prepared into platelet-rich plasma (P < 0.05) . The levels of platelet derived growth factor AB, transforming growth factor-β, basic fibroblast growth factor and vascular endothelial growth factor in platelet-rich plasma were 3.10, 3.45, 7.17 and 5.45 times of the whole blood, respectively (P < 0.05) . (2) The results of hematoxylin-eosin staining observed that the rate of new born, new centumum formation, new alveolar bone formation, and new periodontal ligament tissue formation height at 2 weeks in the autologous platelet gel-collagen group showed no significant difference from the control group (P> 0.05) . At 4 and 8 weeks, all above indexes in the autologous platelet gel-collagen group were significantly higher than those in the control group (P < 0.05) . (3) Results of immunohistochemical staining revealed that at 2 weeks, bone morphogenetic protein-2 in the autologous platelet gel-collagen group began to express, and the expression of bone morphogenetic protein-2 was highest at 4 weeks (P < 0.05) , and the positive expression was weakened at 8 weeks (P> 0.05) . (4) Our results clarify that autologous platelet gel-collagen bioactive composite membrane can significantly promote the regeneration of new tooth, which is associated with the expression of bone morphogenetic protein-2, and reduce the repair time after periodontal tissue defect.