1.Epirubicin hydrochloride combined with capecitabine tablets on serum OPN,VEGF-C and its efficacy in triple-negative breast cancer
Danying XU ; Yan YUAN ; Fuming LI
Chinese Journal of Biochemical Pharmaceutics 2016;36(11):118-120
Objective To explore epirubicin hydrochloride combined with capecitabine tablets on serum levels of osteopontin (OPN), vascular endothelial growth factor C ( VEGF-C) and its efficacy in the treatment of patients with triple-negative breast cancer.Methods 80 cases triple-negative breast cancer were selected, randomly divided into two groups, 40 cases in each group.The control group were given epirubicin treatment, joint group on the basis of the control group were combined with capecitabine, 21 days for a course of treatment, four courses of treatment.The serum levels of OPN and VEGF-C pre-and post-treatment were detected, the efficacy and adverse reactions were observed.Results Compared with before treatment, the serum levels of OPN and VEGF-C in two groups were lower post-treatment(P<0.05).Compared with control group, the OPN and VEGF-C levels in joint group were lower (P<0.05), the efficacy was higher and the adverse reaction was lower (P<0.05).Conclusion The epirubicin hydrochloride combined with capecitabine tablets could reduce serum OPN and VEGF-C levels in the treatment of patients with triple-negative breast cancer, with high security.
2.Effects of Fuzheng Xiaoai decoction joint tamoxifen on serum sex hormone and endometrial thickness with breast cancer
Fuming LI ; Danying XU ; Yan YUAN ; Yue LIU ; Shuzhen FANG
Chinese Journal of Biochemical Pharmaceutics 2017;37(3):122-124,127
Objective To analyze the effects of Fuzheng Xiaoai decoction joint tamoxifen on serum sex hormone and endometrial thickness with breast cancer.Methods 124 patients with breast cancer were divided into control group and research group by lot drawing method, all as 62 cases, treated with the same surgery, control group was treated with tamoxifen, research group was treated with Fuzheng Xiaoai decoction based on control group, the sex hormones, endometrial thickness, tumor markers, immune function and complications were compared between two groups.Results The prolactin (PRL), progesterone (P), estradiol (E2) of research group were all lower than control group, the difference were statistically significant (P<0.05).The endometrial thickness of research group [(8.61+1.07) mm]was lower than the control group [(9.74+1.21) mm](P<0.05).The tumor markers, immune function of research group were better than that of control group (P<0.05).The complications was no difference between two groups. Conclusion Fuzheng Xiaoai decoction joint tamoxifen can regulation the serum levels of sex hormone, relieve tamoxifen-induced endometrial thickening, can improve tumor markers and immune function .
3.Study on Cellular Immune Responses of DNA Vaccine, rAd5 and rMVA Expressing SIV Gag/Env Gene Combined Immunization in Mice.
Xiaozhou HE ; Danying CHEN ; Wandi WANG ; Ke XU ; Yi ZENG ; Xia FENG
Chinese Journal of Virology 2016;32(2):170-178
Therapeutic HIV vaccine was considered as a hopeful curative method for AIDS patients. However, there is still no suitable HIV animal model for vaccine study since the difference in the immune system between human and animals. To evaluate the therapeutic effect of combined immunization strategy with multiple vector vaccines in macaque models. Plasmid DNA, recombinant Ad5 and MVA vaccines which expressing SIV gag and env genes were constructed. Sequential and repeated immune strategy were applied to immunize mice with these three vaccines. Cellular immune responses in mice immunized with these three vaccines were measured by ELISPOT test in vitro and CTL assay in vivo. The results were analyzed and compared with different antigen combination, order of vaccines and intervals to choose a suitable immunization strategy for macaque immunization in future. It indicated that strong SIV-Gag/Env-specific cellular immune responses were induced by these three vector vaccines. It laid a foundation for evaluating the therapeutic effect of combined immunization strategy with multiple vector vaccines in SIV infected macaque models.
AIDS Vaccines
;
administration & dosage
;
genetics
;
immunology
;
Adenoviridae
;
genetics
;
metabolism
;
Animals
;
Antibodies, Viral
;
immunology
;
Female
;
Gene Products, env
;
administration & dosage
;
genetics
;
immunology
;
Gene Products, gag
;
administration & dosage
;
genetics
;
immunology
;
Genetic Vectors
;
genetics
;
metabolism
;
HIV Infections
;
immunology
;
prevention & control
;
virology
;
Humans
;
Immunization
;
Mice
;
Mice, Inbred BALB C
;
Simian Immunodeficiency Virus
;
genetics
;
immunology
;
Vaccines, DNA
;
administration & dosage
;
genetics
;
immunology
4.Effects of Chinese herbal medicine Neiyi Recipe-medicated serum on angiopoiesis of endometriosis in the chick chorioallantoic membrane model.
Ling XU ; Qiaoling ZHOU ; Jie HAN ; Danying ZHANG ; Huijuan DONG ; Zailong CAI ; Chaoqin YU ; Dongxia ZHAI
Journal of Integrative Medicine 2012;10(7):800-6
To compare angiopoiesis ability of eutopic and ectopic endometrial tissue isolated from women with endometriosis and endometrium isolated from women without endometriosis (control), and to explore the inhibitory effects of medicated serum of Neiyi Recipe, a compound traditional Chinese herbal medicine.
5.Research on the construction of innovative experimental teaching system of pathogenic biology and immunology
Zhenjie HUANG ; Danying QIU ; Xiuxiu XU ; Shuzeng CHEN ; Lixia CHEN ; Yonghua XIE
Chinese Journal of Medical Education Research 2021;20(4):442-444
Relying on the practice platform of P2 laboratory, researchers reform and explore the contents and forms of experimental teaching for pathogenic biology and immunology, creating an innovative experimental teaching system suitable for training medical students' professional quality and innovative ability. By improving teaching conditions (construction of P2 laboratory), improving teaching methods and innovating experimental teaching contents, and introducing comprehensive design experiment, students' consciousnesses of service and biosafety have been strengthened, meanwhile, their ability to analyze and solve problems has been improved, and their critical and creative thinking has been cultivated.
7.Analyzing the monitoring results of noise hazards in workplace of key industries in Guangdong Province in 2019
Jianyu GUO ; Danying ZHANG ; Guoyong XU ; Maosheng YAN ; Zhipeng HE ; Hua YAN ; Jiayu LIU ; Bin XIAO
China Occupational Medicine 2023;50(2):175-180
8.Effects of long-term high-fat diet on bone mineral density and intestinal flora
Kan XU ; Nan WU ; Lihong GAO ; Lin ZHU ; Danying ZHANG ; Yu HU
Chinese Journal of Endocrinology and Metabolism 2022;38(8):665-673
Objective:To investigate the effects of long-term high-fat diet on bone mineral density and intestinal flora in mice.Methods:Sixteen male C57BL/6 mice were randomly divided into control group (NC group) and high-fat group (HF group). After 24 weeks of high-fat feeding, biochemical indicators such as blood glucose and blood lipids were detected, bilateral femurs were taken and bone microstructure was analyzed with micro-computered tomography (micro-CT), and changes of intestinal microbial composition and proportion were revealed using 16S rDNA sequencing technology.Results:Compared with the control group, the serum total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) in HF group were significantly increased. Micro-CT uncovered that the bone mineral density (Tb.BMD), bone volume fraction (BV/TV) and the number of trabecular bone (Tb.N) decreased, yet structural model index (SMI) and the trabecular fraction (Tb.Sp) increased in the HF group mice. The gut microbiota 16S rDNA sequence analysis showed that the proportion of Proteobacter was significantly increased and the proportions of pachycete, warty microbacterius, and actinomycete were reduced in HF group at the phyla level. The proportion of Bacteroidetes S24-7_norank in the NC group was significantly higher than that in the HF group, and the multilevel discriminant analysis of species differences (LEfSe) identified that the difference was significant, yet the proportion of Bacteroides, Pseudo-Prevotella, Desulfovibrio, Altobacter, and Helicobacter in the HF group were higher than those in the NC group, which were significant differences in Altobacter and Helicobacter at genus level.Conclusion:Long-term high-fat feeding can cause the destruction of femoral trabecular structure, decrease in the number of trabeculus bones, and bone mineral density in C57BL/6 mice. It also leads to significant changes in the composition and proportion of the intestinal flora.
9. Influencing factors of lower back/waist work-related musculoskeletal disorders among workers in a shipyard
Danying ZHANG ; Xinqiang NIE ; Ning JIA ; Lingling XU ; Mingliang LIAO ; Lei SU ; Ziye LI ; Hua YAN ; Zhongxu WANG
China Occupational Medicine 2020;47(01):41-47
OBJECTIVE: To determine the prevalence and influencing factors of work-related musculoskeletal disorders(WMSDs) among workers in a shipyard. METHODS: A total of 496 workers in a large shipyard in Guangdong Province were selected as research subjects using the convenient sampling method. The Questionnaire of Musculoskeletal Disorders was used to investigate the prevalence of WMSDs in various regions of the body in the past year.Multivariate logistic regression was used to analyze the potential influencing factors of WMSDs in the frequently affected body parts. RESULTS: The prevalence of WMSDs was 70.2%(348/496). The prevalence rates of WMSDs in different body regions were: lower back/waist(43.1%), neck(29.4%), shoulder(29.0%), hand/wrist(25.4%), knee(22.4%), hip/leg(14.3%), ankle/foot(12.1%), upper back(11.3%) and elbow(9.3%). Multivariate logistic regression analysis results showed that increased risk of lower back/waist WMSDs occurred in workers who carry heavy objects>5.0 kg, who work in uncomfortable positions, who repeatedly perform the same work every day, and who repeatedly perform the same action using the lower limbs and ankles(P<0.05). Employees working ≤8 hours or more than 8 hours per day had higher risk of developing lower back/waist WMSDs compared with workers working 8-10 hours per day(P<0.05). CONCLUSION: The prevalence of WMSDs among workers in the shipyard is high.The lower back/waist WMSD is the most common one. The influencing factors include work organization and adverse ergonomic factors.
10.Correlation between work fatigue and work-related musculoskeletal pain or injury in the occupational population in China
Qing XU ; Huadong ZHANG ; Ruijie LING ; Yimin LIU ; Gang LI ; Zaoliang REN ; Yan YIN ; Hua SHAO ; Hengdong ZHANG ; Bing QIU ; Meibian ZHANG ; Dayu WANG ; Qiang ZENG ; Rugang WANG ; Jianchao CHEN ; Danying ZHANG ; Liangying MEI ; Yongquan LIU ; Jixiang LIU ; Chengyun ZHANG ; Tianlai LI ; Ning JIA ; Zhongxu WANG
China Occupational Medicine 2023;50(2):133-139