Objective To investigate the expression of far upstream element binding protein 1 (FUBP1) gene in gastric cancer,and analyze its effect on proliferation and invasion of gastricc cancer cells.Methods The expression of FUBP1 in gastric cancer tissues was detected by real-time PCR.The relationship between FUBP1 expression and clinic pathological factors of gastricc cancer was analyzed by one-way ANOVA.The silence vector for FUBP1 was constructed and transfected into gastricc cancer SGC7901 cells,and the transfected effects were then detected by real-time PCR.The influence of FUBP1 silence on cell proliferation and invasion in SGC7901 cells were detected by CCK8 method and Transwell assay.Results FUBP1 expression was increased markedly in gastricc cancer tissues.Following the depressing of differentiation and the increase of invasion depth and metastatic lymph nodes,the FUBP1 expression was up-regulated in primary gastric carcinoma significantly.The pS-FUBP1 vector could silence the FUBP1 expression in SGC7901 cells.FUBP1 silence inhibited SGC7901 cell proliferation amd invasion.Conclusion FUBP1 was high-expressed in gastricc cancer,and related with genesis and progress of gastric cancer,silence of FUBP1 expression can inhibit the cell proliferation and invasion in gastric cancer cells.

Objective:To assess the effectiveness and safety of retinol topical serum combined with Q-switch ruby laser in the treatment of periorbital hyperpigmentation (PH).Methods:From October 2021 to December 2021, 30 patients with PH were treated in the Department of Dermatology, the Fifth Affiliated Hospital of Sun Yat-sen University, including 7 males and 23 females, aged 24-33 years, with an average age of 28.9 years. The patients were divided into a combination therapy group of 20 cases and a laser therapy group of 10 cases. In the combined treatment group, 20 subjects received a Q-switch ruby laser once a month, three times in total, and the retinol topical serum were used after the treatment for three months; the others were treated solely with laser.Results:In the combined treatment group, there was significant difference in visual analogue scale (VAS) before and after the use of retinol topical serum ( t=3.21, P=0.005) and in Sadick tear trough rating scale before and after treatment ( t=2.67, P=0.015). After therapy, 4 cases obtained good improvement and 13 cases obtained some improvement in the overall evaluation of the investigator; 17 patients had good improvement and some improvement. The overall satisfaction scores of 17 subjects were very satisfied. In the laser therapy group, there was no statistically significant difference in VAS before and after treatment in the simple laser treatment group ( t=0.29, P=0.782), and there was no statistically significant difference in the Sadick tear trough rating scale ( t=1.56, P=0.154). Among them, in the overall evaluation of the researchers, 1 case (10%) achieved good improvement, 3 cases (30%) achieved some improvement. The overall satisfaction score of 4 subjects was very satisfactory. The combination therapy group had the highest effective rate and satisfaction, and the difference between the two groups was statistically significant (χ 2=14.089, P=0.002). Conclusions:The application of retinol topical serum combined with Q-switched ruby laser in periorbital hyperpigmentation is safe and effective, and is worthy of clinical promotion.

The aim of this work is to discover the inhibitory mechanism of tea peptides and to analyse the affinities between the peptides and the angiotensin-converting enzyme (ACE) as well as the stability of the complexes using in vitro and in silico methods. Four peptide sequences identified from tea, namely peptides I, II, III, and IV, were used to examine ACE inhibition and kinetics. The half maximal inhibitory concentration (IC

BACKGROUND: Findings on the association of genetic factors and colorectal cancer (CRC) survival are limited and inconsistent, and revealing the mechanism underlying their prognostic roles is of great importance. This study aimed to explore the relationship between functional genetic variations and the prognosis of CRC and further reveal the possible mechanism. METHODS: We first systematically performed expression quantitative trait locus (eQTL) analysis using The Cancer Genome Atlas (TCGA) dataset. Then, the Kaplan-Meier analysis was used to filter out the survival-related eQTL target genes of CRC patients in two public datasets (TCGA and GSE39582 dataset from the Gene Expression Omnibus database). The seven most potentially functional eQTL single nucleotide polymorphisms (SNPs) associated with six survival-related eQTL target genes were genotyped in 907 Chinese CRC patients with clinical prognosis data. The regulatory mechanism of the survival-related SNP was further confirmed by functional experiments. RESULTS: The rs71630754 regulating the expression of endoplasmic reticulum aminopeptidase 1 ( ERAP1 ) was significantly associated with the prognosis of CRC (additive model, hazard ratio [HR]: 1.43, 95% confidence interval [CI]: 1.08-1.88, P = 0.012). The results of dual-luciferase reporter assay and electrophoretic mobility shift assay showed that the A allele of the rs71630754 could increase the binding of transcription factor 3 (TCF3) and subsequently reduce the expression of ERAP1 . The results of bioinformatic analysis showed that lower expression of ERAP1 could affect the tumor immune microenvironment and was significantly associated with severe survival outcomes. CONCLUSION: The rs71630754 could influence the prognosis of CRC patients by regulating the expression of the immune-related gene ERAP1 . TRIAL REGISTRATION No. NCT00454519 ( https://clinicaltrials.gov/ ).
Humans ; Prognosis ; Genotype ; Polymorphism, Single Nucleotide/genetics* ; Quantitative Trait Loci ; Colorectal Neoplasms ; Tumor Microenvironment ; Aminopeptidases/metabolism* ; Minor Histocompatibility Antigens/genetics*