1.Applied technology improving bio-compatibility of hemodialysis membrane
Changjun HOU ; Shujia ZHENG ; Danqun HUO ; Wenbin ZHANG ; Yuanliang WANG
Chinese Medical Equipment Journal 1993;0(05):-
As a means to substitute the function of kidney, hemodialysis therapy has been applied to clinical therapy. The bio-compatibility of dialyser, which is the key of part of artificial kidney, is a determining factor for the therapy. This paper describes dialyser and the methods of improving bio-compatibility of dialyser.
2.Characterization of anticoagulant biomaterial and its development.
Bingcan CHEN ; Danqun HUO ; Jiajia RAO ; Changjun HOU ; Mingyuan LI
Journal of Biomedical Engineering 2005;22(2):428-432
Good anticoagulant biomaterials need good surface chemical properties, good mechanics performances and particularly good characteristics of biocompatibility, including tissue compatibility and hemocompatibility. In order to understand with greater clearness the anticoagulant biomaterial, we have to characterize them by different methods. In this paper, the approaches to assessing and displaying the characteristics of anticoagulant biomaterial are reviewed in three aspects, namely the surface chemical properties and structure, the mechanics performances the and the biocompatibility of anticoagulant biomaterial.
Anticoagulants
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Biocompatible Materials
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chemistry
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Blood Coagulation
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drug effects
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Humans
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Materials Testing
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Prostheses and Implants
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adverse effects
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Prosthesis Design
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Surface Properties
3.Cloning of a phyA gene and its over expression in E. coli.
Danqun HUO ; Shoucheng FAN ; Yunru ZHANG ; Shoujun FAN
Journal of Biomedical Engineering 2007;24(1):176-181
This research amplified the phyA gene with the designed and synthesized primers specific for the phyA gene full-length coding sequence. The phyA gene was from Aspergillus niger F246 by the polymerase chain reaction(PCR), which is selected and identified in our laboratory. After sequncing the coding sequence, it was confirmed that the construction of cloning vector was succeeded. The phyA gene fragment was recovered from the pMD18T-phyA and ligated with prokaryotic expression vector pET30a+ to construct the recombinant expression plasmid pET30a+ -phyA. It was expressed with IPTG induction in E. coli for high efficiency. A new protein band with apparent molecular weight 50 kDa was detected in the lysate of the transformed cell by using SDS-PAGE. The amount of the soluble fusion protein was about 40% of large intestine bacillus soluble protein of transformed cells, estimated by absorbance scanning of SDS-PAGE and protein quantitation. It's phytase activity was eight times over the natural phyase. So this research provides the basis of the study on obtaining large and high active phytase and developmant of the new microbial ecologicalagent.
6-Phytase
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biosynthesis
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genetics
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Aspergillus niger
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enzymology
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genetics
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Base Sequence
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Cloning, Molecular
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Escherichia coli
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genetics
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metabolism
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Genes, Fungal
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Genetic Vectors
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Molecular Sequence Data
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Recombinant Fusion Proteins
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biosynthesis
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genetics
4.Progress in the treatment of stained teeth.
Danqun HUO ; Guo XIE ; Changjun HOU ; Jia LIU ; Chunyue HUANG ; Zuoyun HE
Journal of Biomedical Engineering 2004;21(3):512-515
The treatment of stained teeth has been one of the striking aspects of stomatology and esthetic dentistry. Based on detailed data and references, this article introduces the types of stained teeth and the main treatment methods including strong point, weakness, limitation of the usage, result, and the relevant mechanisms. It addresses the researches on problems in the treatment of stained teeth. Also in this paper is envisaged what will be done to treat the stained teeth in future.
Dental Veneers
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Humans
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Tooth Bleaching
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Tooth Discoloration
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etiology
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therapy
5.Study on all-round evaluation of biocompatibility of biomaterial.
Danqun HUO ; Dongni ZHAN ; Changjun HOU ; Wenbing ZHANG ; Shujia ZHENG ; Yanrong REN ; Bingcan CHEN
Journal of Biomedical Engineering 2006;23(6):1350-1354
Biocompatibility has always been the focal point in the study of biomaterials applied to medical apparatus. But at present, the standard system hasn't formed completely to evaluate the biomaterials. In recent years scientists tend to use general standard of evaluation. In this article, based on the recalcification time, the adhesion of platelets as well as the total quantity of plasma protein and some other evidences, combining with the Analytic hierarchy process (AHP), the method of general evaluation on the biocompatibility of anticoagulant biomaterials was discussed.
Anticoagulants
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analysis
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Biocompatible Materials
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standards
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Blood Proteins
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analysis
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Humans
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Materials Testing
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methods
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standards
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Platelet Adhesiveness
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drug effects