Aim To establish the rapidly detecting method of glucose concentration in brain microdialysate.Method Using high-performance liquid chromatography (HPLC) with electrochemical detector (ED) and Nano-Copper screen-printed electrode (NCSE100)-Flow injection analysis the glucose concentration in brain microdialysate were detected.Result The related coefficient (r) of glucose standard curve was 0.9995. The variation coefficient (CV) within a day was 4%~7%. The striatum extracellular glucose concentration in rats was(2.4?0.8) mmol?L-1. These results indicated that our method was reliable.Conclusion Using HPLC with ED, NCSE100, and flow injection analysis the glucose concentration in brain microdialysate were detected, which has certain applied worth.

Ligustrazine which has extensive pharmacological effects has been clinically used in the treatment of cardiovascular and cerebrovascular diseases,respiratory diseases,urinary diseases,cirrhosis,cancer,etc.Research of protective effects of Ligustrazine on spinal cord injury has recently increased year by year.Numerous clinical trials and animal experiments suggest that Ligustrazine can play a role on ischemic cerebrovascular disease, degenerative brain disease,epilepsy,spinal cord injury and so on,whose main mechanisms include inhibiting of apoptosis and inflammation,enhancing the body’s antioxidant level,improving hemorheology,increasing expression of neurofilament protein and neurotrophic factors,and regulating balance of the ion intracellular and extracellular.Through consulting the relevant literature since 2003,this article reviews the protective roles and mechanisms of Ligustrazine on cerebra and spinal cord injury for the past 10 years.

It was proved that Phenolic alkaloids from Menisphermum dauricum (PAMD) has extensive pharmacological effects, manifested as affecting electrophysiology of the heart, resisting cardiac arrhythmia, inhibiting myocardial infarction, protecting nverves in cerebral ischemia, exerting resisting action in thrombogenesis, dementia, and tumor, and improving learning disorder, and so on. We made an overall sum up of researching progress on PAMD pharmaceutical actions in this article.

Aim To provide an approach to drug filtration and the research into damage of brain cell caused by senile retrograde affection and oxidative stresses,a model of increased extracellular hydroxyl radical in striatum of rats was established by intracerebral perfusion of 6-hydroxy dopamine(6-OHDA).Method Microdialysis was used to establish the model,hydroxyl radicals captured by salicylic acid yielded 2,3-dihydroxy benzyl acid(2,3-DHBA) and 2,5-dihydroxy benzyl acid(2,5-DHBA) which were measured by HPLC-ED.Results After 75 minuets of the perfusion with 6-OHDA in brain of rats,the levels of 2,3-DHBA and 2,5-DHBA in the model group increased to 6.6 and 3.4 times compered with the control group respectively;and the 2,3-DHBA was still higher than that of the control group in the whole course of observation(P

0.05).Conclution After at a time sampling the levels of hydroxyl free radical and monoamines neurotransmitters and their metabolites from brain micronilysis of freely moving rats during salicylate sodium-Ringer's solusion perfusion can be simultaneously measured by HPLC-ED.

Aim To investigate the effects of masculine drug Duxil on striatum extracellular acetylcholine(ACh),glucose and lactic acid(LD) levels in energy metabolism impairment rats induced by intracerebral perfusion with sodium azide(NaN3).Methods Rats were divided into three groups:control,model and Duxil groups.Rats of Duxil group were treated with 10 mg?kg-1?d-1 Duxil for 14 d.Other two groups were treated with water for 14 d.After 2 wk microdialysis technique was adopted and the rats were perfused with NaN3 in striatum and continuously measured striatum extracellular ACh,glucose and LD levels in freely moving awake rats.Results During perfusion(90 min) and stoping perfusion(180,240,360 min)period with NaN3 striatum extracellular ACh levels in the model group were significantly lower than those in the control group(P

Aim To establish the model of oxidative damage in brain of Parkinsons disease(PD)rats induced by levodopa(L-DOPA)with microdialysis technique.Methods PD model rats were induced by intracerebral injection of 6-hydroxyl dopamine(6-OHDA)and were perfused in brain with L-DOPA by using microdialysis technique.Salicylic acid can capture hydroxyl radicals in brain,then yield 2,3-dihydroxy benzyl acid(2,3-DHBA)and 2,5-dihydroxy benzyl acid(2,5-DHBA).Extracelluler dopamine(DA)and its metabolites,2,3-DHBA and 2,5-DHBA in striatum of rats were measured by HPLC-ED before and after L-DOPA treatment.Results Both 2,3-DHBA and 2,5-DHBA in model group were significantly higher than those in control group at 6 and 7 time points respectively(P

AIM To establish the method of continuous measuring extracellular Acetylcholine and Choline levels in[FQ(10*2。46,X-WZ]-striatum of freely moving awake rats. METHODS The microdialysis and high performance liquid chromatography(HPLC)-post column Immobilized enzyme reactor(IMER)-electrochemical detection(ED) were used to continuously measure extracellular acetylcholine and choline levels in striatum of freely moving awake rats. RESULTS Extracellular acetylcholine and choline levels were higher at the beginning of perfusion, then decreased gradually. The ACh concentration reached a stable level after 240 min while the Ch continuously decreased in the whole perfusion period of 450 min. The detection limit for ACh and Ch, at a S/N ratio of three, was found to be 150 fmol per injection. In the range of 0.125～1.0 ?mol?L -1 , the relation between the amounts and the peak amplitude was linear. CONCLUSION HPLC-IMER-ED is a sensitive and stable method to measure the ACh and Ch. Coupled with microdialysis, it can been successfully used for monitoring the extracellular ACh and Ch levels of freely moving awake rats. -

Objective To study the sensitization of Qingkailing I njections produc ed in five pharmaceutical factories and the sensitization of each composition an d adminicle of Qingkailing Injections on the Beagle dogs by intravenous injectio n. Methods By intravenous injection with Qingkailing In jections produced by the five pharmaceutical factories and all the compositions and adminicles into the Beagle dogs, the behavior changes of the dogs were observed and the content of serum histamine, I gE, IgG, IgM, ECP and IL-4 were detected. Results The result was determined ac cording to the indices of both behavior changes and the serum histamine, IgE, Ig G, IgM, ECP and IL-4. Qingkailing Injections produced in three pharmaceutical f a ctories and the compositions, extract of gardenia and cholalic acid were positi ve in induc ing the anaphylactoid reaction. Conclusion The Qingkail ing Injections produced in three pharmaceutical factories can induce anaphylactoid reaction. The extrac t of gardenia and cholalic acid may be the relevant substance of anaphylactoid r eaction.