Objective To investigate the relationship of the expression characteristics of CD4 + CD25 + Foxp3 + Treg cells and CD4 + IL-17 + Th17 cells and the progress of the elderly patients with laryngeal cancer.Methods Sixty elderly patients diagnosed as laryngeal cancer in our hospital from January 2013 to June 2014 were chosen.Among them,28 cases had lymph node metastasis and 32 without.Thirty cases without laryngeal diseases were chosen as control.The peripheral blood of elderly patients with laryngeal cancer and normal controls was extracted.The expression levels of Treg and Th17 cells in all groups were detected with flow cytometry,and the expression changes of Foxp3,RORγt in peripheral blood mononuclear cell (PBMC) were detected with real time quantitative PCR.Results Compared with the control group,CD4 + CD25 + Foxp3 + Treg and CD4 + IL-17 + Th17 cell percentage,Treg and Th17 cells specific transcription factor Foxp3,RORγt expression levels in elderly laryngeal cancer patients without lymph node metastasis were significantly higher (t=9.817,P=0.018; t=12.120,P=0.009; t=6.090,P=0.023; t=7.130,P=0.028).Compared with elderly laryngeal cancer patients without lymph node metastasis,Treg and Th17 cell percentage and Foxp3,RORγt expression levels were significantly increased (t =9.070,P =0.014; t =12.140,P =0.009; t =10.130,P =0.009 ; t =13.070,P =0.008),and the ratios of Th17/Treg and Foxp3/RORγt were significantly lower (2.401 ± 0.614 vs 3.763 ± 0.959 ; 0.401 ± 0.075 vs 0.563 ± 0.091 ; t =9.070,P =0.014 ; t =11.140,P =0.007) in elderly laryngeal cancer patients with lymph node metastasis.Conclusion In elderly patients with laryngeal cancer,Treg cells and Th17 cells imbalance is positively correlated to disease progression.Therefore,monitoring and correcting the expression levels of Treg and Th17 cells may be important for the diagnosis,treatment and prognosis of the elderly patients with laryngeal cancer.

Objective To investigate the effect of cavity thickness, area and distance under the bolus upon the dose in the superficial tissues. Methods An accelerator model was constructed based on Geant4.The model accuracy was validated by the comparison of the calculated data with the measured data. A 30×30×30 cm3 water phantom with the upper surface located at the isocenter level and a 30×30×1 cm3 water film were constructed. Different models with the water film close to or different cavities with the water phantom were established. Under the 10×10 cm2 field with 6 MV X-ray beam,the central axis depth dose distribution and the lateral dose profiles at a depth of 0. 1 cm ( profile1) of the models with different cavities were calculated. The calculated data of different model with the water film close to or different cavities with the water phantom were statistically compared. Results When the cavity thickness was ≤ 0. 5 cm, the cavity exerted slight effect upon the depth of maximum dose ( Dmax ) and superficial dose. As the cavity thickness was increased,the Dmax was also increased,the PDD at 0. 1 cm ( PDD1) was decreased rapidly and the profile1 was increased from the cavity center to the edge. Along with the increase of cavity area,the Dmax was initially increased and then decreased,whereas the PDD1 was first decreased followed by an increase. When the cavity area was small,the profile1 was gradually increased from the cavity center to the edge. When the cavity area was large,the profile1 was initially decreased and subsequently increased. When the distance was ≥0. 2 cm,it was qualified for the clinical requirement and it exerted no effect when the distance was≥1. 0 cm. The profile1 distant from the cavity was not affected. Conclusion The cavity under the bolus should be minimized to reduce the cavity thickness,area and distance as possible.

Objective To evaluate the effect of the thickness and area of the cavity between bolus and skin upon the dose deposition in the shallow tissues.Methods The linear accelerator head model of 6 MV X ray was constructed using Geant4,which simulated the accelerator working condition of 10 cm× 10 cm field and recorded the information of emergent particles as phase space file.A water phantom of 30 × 30 × 30 cm3 was designed at the SAD level.A 30 cm × 30 cm × 1 cm water film was constructed adjacent to or with different cavities to the upper surface of the phantom.The phase space file was utilized as particle source to simulate the particle transport process.The axis depth dose distribution and lateral dose profiles in the center area of the field at a depth of 1 mm,5 mm,9 mm,15 mm and 21 mm of the phantom were obtained,respectively.The simulated data obtained from water film with different cavities were compared with those of adjacent to the water phantom.Results When the cavity was ≤ 5 mm,the cavity exerted slight effect.When the thickness was increased,the maximum dose depth (Dmax) was increased,the PDD at Dmax (PD Dmax) became less,the depth of influenced lateral dose profiles was increased and the dose in the center area was decreased.Along with the increase of cavity area,the Dmax was initially increased and then decreased,the PD Dmax was at first decreased and subsequently increased,the depth of influenced lateral dose profile was initially increased and then decreased,the dose in the center area was first decreased and subsequently increased.The lateral dose profile distant from the cavity or located at a depth ≥ 15 mm was almost not affected by the cavity.Conclusion The thickness of the cavity between bolus and skin should be less than 5 mm and the cavity area should be small as possible.

OBJECTIVE: To understand the distribution of allergens in patients with allergic rhinitis (AR) in Quanzhou, for the diagnosis, prevention and treatment. METHOD: Twenty standardized allergen underwent skin prick testing (skin prick test, SPT) were used for 1236 patients with suspected AR patients in Quanzhou, and the different age, gender and severity in patients with allergen distribution differences of positive rate were analyzed. RESULT: The top 5 allergens with positive skin tests were D. pteronyssinus (66.7%), D. farinae (65.5%), Blattodea (32.8%), Sea crab (18.2%), and Sea lobster (16.8%). The positive rate of SPT in the minor groups and adult groups had significant difference (P < 0.05). Gender difference between the positive rate of SPT had no significant difference (P > 0.05). There was significant difference between the positive rate in patients with a family history and history of asthma and patients without above two history (P < 0.05). CONCLUSION Dust Mite, Blattodea, Sea crab. Sea lobster are the most important allergens in quanzhou. Different ages of the patients with SPT positive rates vary, a family history, history of asthma patients with positive rate is higher than patients without a family history of asthma history, high positive rate.
Adolescent ; Adult ; Aged ; Allergens ; analysis ; immunology ; Animals ; Child ; Child, Preschool ; China ; epidemiology ; Female ; Humans ; Male ; Middle Aged ; Rhinitis, Allergic ; Rhinitis, Allergic, Perennial ; diagnosis ; epidemiology ; immunology ; Skin Tests ; Young Adult