Investigate paper growth tendency of three annual meetings of Chinese Society of Thoracic and Cardiovascular Surgery in order to promote a sound development of this field in China.Statistical analysis shows numbers and growth tendency of papers of annual meetings of Chinese Society of Thoracic and Cardiovascular Surgery from 2013 to 2015 in terms of regions and provinces,author working unit and other indicators of acadenic topics.The number of papers in east China ranked first,accounting for 33.24 ％ of total numbers.Papers from the top ten provinces accounted for about two-thirds of the total number.Hospital author manuscript is the most important source,accountiug for 99.68％.Cardiovascular surgery,thoracic surgery,extracorporeal circulation and nursing are the four key parts under Chinese Society of Thoracic and cardiovascular surgery,the number of papers and Cardiovascular Surgery dominants,accounting for 55.1％ of the total;minimum number of papers went to nursing,accounting for 2.79％ of the total,while it remained fastest growth rate of 139％.The numbers of papers vary and gap among different regions,which should be valued in development.Papers on nursing keep minimum number,but the growth rate is the highest,with great potential to improve.

Objective To investigate the characteristics of children ametropia in the development process ,predict and analyze the refractive state with the measurement data of refractive factors on the children aged from 5 to 13 years old .Methods 461 children (922 eyes) ,aged from 5 to 13 years old ,were selected from outpatients who went to ophthalmology clinic from January 2011 to September 2012 .The static diopter was measured by the optometry with the continuous mydriasis of 5 days using the ophthalmic gel of 1% atropine .And the biological measured values of refractive factors were obtained by the A type ultrasonic and keratome-ter .Meanwhile ,the parameters including the height (H) ,weight and the dominant eye were recorded to analyze the characteristics of children ametropia in the development process ,and then the equation between the diopter and the correlative factors including the measurement data of refractive factors and growth factors was established by the stepwise multiple regression analysis .Results (1) The myopia degree of the right eye static diopter was significantly deeper than the left (P<0 .05) .In the group in which the left eye was dominant ,the difference of the left eye and right eye refractive factors was not statistically significant (P>0 .05) .In the group in which the right eye was dominant ,the difference of the left eye and right eye refractive factors was not statistically significant (P>0 .05) except the instance in which K1 of the right eye was bigger than the left eye(P<0 .05) .(2) In the period of 5 to 13 years old ,the myopia developing trend of girls was earlier than boys .(3) The ocular axis length(AL) and H development had a close relationship ,and the correlation equation of them was AL =16 .135+0 .052H(r=0 .993) .(4) The multiple linear regression equation for the relationship between the static diopter (D) ,ocular AL and mean value of the corneal curvature (K) was D=84 .271-0 .937K -1 .878AL(r=0 .871) .Conclusion The ocular AL and H development of children have a close relationship .The static diopter(D) of the children aged from 5 to 13 years old can be predicted by the ocular axis length(AL) and corneal curvature(K) , w hich can be used to prevent myopia reasonably .

Objective To construct GST/HIF1α fusion protein expression vector and induce its expression in Escherichia coli (E.coli). Methods The coding sequence of hypoxia inducible factor-la (HIF-la) and its deletion fragments were amplified from the plasmid pcD-NA3.1-HIF-la by PCR and inserted into pGEX-4T-2 by BamHI and Not I. The positive recombinanls were identified by restriction endonu-clease digestion and DNA sequencing. Then they were transformed into E.coli BL21, induced by IPTG and identified by SDS-PAGE and Western blot. Results The prokaryotic expression plasmid pGEX-4T-2-HIF-lα and its deletion mutants were successfully constructed and confirmed by enzyme digestion and sequencing. The desired CST/HIF-1α fusion proteins were expressed and confirmed by Western blot. Conclusion The prokaryotic expression plasmid of HIF-la and its deletion mutants were successfully constructed and the expression of fu-sion proteins was confirmed. This study provides the basis for the further research on purifying HIF-la protein and the biological function of HIF-lα.

Objective To investigate the effect of quinone oxidoreductase 1 (NQO1) and quinone oxidoreductase 2 (NQO2) expression in the prognosis of ovarian carcinoma.Methods The two online databases were used to perform the online analysis on the NQO1 and NQO2 mRNA levels and prognosis of ovarian cancer patients,1 306 cases of ovarian cancer were performed the Kaplan-Meier analysis by using the KaplanMeier plotter (K-M plotter);578 cases of ovarian cancer in the TCGA database were performed the univariate COX regression survival analysis.Results The K-M plotter analysis showed that the NQO1 expression level had no obvious correlation with the prognosis of ovarian cancer (P>0.05),the higher the NQO2 level,the better the prognosis (HR=0.83,P=0.006 2).The COX regression survival analysis showed that the NQO1 expression level had no obvious correlation with the prognosis of ovarian cancer(P>0.05),the higher the NQO2 level,the better the prognosis (P=0.038 29).Conclusion NQO2 expression level has no obvious correlation with the prognosis of ovarian cancer,moreover the higher the NQO2 expression level,the better the clinical prognosis of ovarian cancer.

Objective To investigate the effect of luxS inactivation on the oxidative stress of Streptococcus mutans and perform preliminary analysis of potential mechanism.Methods Strains were grown to mid-logarithmic phase and divided into three groups,one was used as control and inoculated into normal TPY medium,and the other two groups were experimental groups,and there were separately inoculated into TPY containing 58.8 mmol/L hydrogen peroxideor TPY containing 58.8 mmol/L hydrogen peroxide and 0.1 mmol/L 2,2'-dipyridyl.The survival rate of strain was calculated at 0.5,1,and 2 h.All the data were statistically analyzed.Results Compared with the control group,the survival rate of luxS mutation was always higher than standard strain at all pre-determined time inexperimental groups (P＜0.05),and compared with experimental group without iron chelator,the survival rate of strains was not raised with the added of iron chelator (P＞0.05).Conclusion luxS gene is involved in oxidative stress tolerance of Streptococcus mutans,and the oxidative stress tolerance is not achieved by avoiding the toxic effects of the Fenton reaction

AIM: To detect the inhibition effect of nitidine chloride on proliferation of human liver cell L-02 and human kidney cell 293 and the protective effect of acidic fibroblast growth factor(aFGF) on human liver cell L-02 and human kidney cell 293 damaged by nitidine chloride in vitro. METHODS: The MTT assay was used to assess the proliferation of human liver cell L-02 and human kidney cell 293 treated with nitidine chloride.The contents of SOD and MDA and LDH in cultural supernate were measured by ultraviolet spectrophotometry. RESULTS: Nitidine chloride inhibited the proliferation of human liver cell L-02 and human kidney cell 293 in a dose-dependent manner. CONCLUSION: Nitidine chloride has certain toxicity on human liver cell L-02 and human kidney cell 293.aFGF could protect human liver cell L-02 and human kidney cell 293 damaged by nitidine chloride.

Objective To design a FPGA(Field-Programmable Gate Array)-based image & voice signal optical transmission system,which can accomplish video & audio frequency long-distance transmission.Methods FPGA was used as the main processing chip.High speed transceiver TLK2501 and SFP optical module were also used to realize high-speed serial fiber transmission.Results A non-compressed digital video optical fiber transmission system based on the TDM technology was designed.Conclusion The system achieves the real-time transmission of video and voice signals,thus greatly improving the accuracy of the examination and providing references for diagnostic experts.

Objective To explore how to optimize laboratory work flow in regional laboratory information management system (LIS) .Methods Upgraded both software and hardware ,reduced manual operation ,and to avoid the mistake and improve the effi‐ciency .Results Compared to the original process ,the optimized one reduces the time line by 57% .Furthermore ,time cost of other individual processes ,such as patient information input ,sample collection and sample classification were also shortened significantly . Conclusion By work flow rebuilding ,we not only improved the efficiency of laboratory work by saving time cost ,but also reduced the error rate ,which finally satisfied our patients .

Objective To investigate the significance of miR-103 as noninvasive biomarker for diagnosis of nonalcoholic fatty liver disease (NAFLD).Methods The serum samples were collected from healthy subjects and NALFD patients to detect biochemical parameters.NucleoZOL method was used to isolate serum miRNA.SYBR Green qPCR was used for relatively quantitative analysis of miR103a-2.The correlations between miR-103a-2 and biochemical parameters were analyzed by Spearman method.Results Compared with healthy control group,the levels of total cholesterol (TC),triacylglyceride (TG),alanine aminotransferase (ALT) and glutamyl transpeptidase (γ-GGT) were all significantly increased in NAFLD group (P ＜ 0.01).Compared with the NAFLD group accompanying ortholiposis,the levels of TC,TG and low density lipoprotein-cholesterol (LDL-C) were all significantly increased in the NAFLD group accompanying dyslipidemia (P ＜0.01).Compared with the healthy control group,the level of serum miR-103a-2 increased in both NAFLD with ortholiposis group (P ＜ 0.05) and NAFLD with dyslipidemia group (P ＜ 0.01).The level of serum miR-103a-2 was positively correlated with serum TC (r =0.495,P =0.001).Conclusion Serum miR-103a-2 may be a more sensitive parameter for noninvasive screening of NAFLD than the parameters of blood lipid.

Objective To construct the expression plasmid of human PAK4 gene and identify its recombinant protein expression.Methods Total RISA was extracted from human breast cancer MCF-7 cells.The hPAKA coding sequence was amplified by polymerase chain reaction (PCR) method and subcloned into pEBG vector.After the target region was sequenced.the plasmid was transfected into HEK293 cell line.The expression of the recombinant plasmid in HEK293 cells was proved by Western blot.Results hPAKA had been constructed into the expressing vector pEBG successfully.The length of the fragment was 1 800 bp,identified by restriction enzymes digestion.The expression of pEBG-hPAK4 fusion protein was detected by Western blot,with a molecular weight 94 KDa and was pulled down by Qutathione Sepharose 4B.Conclusion The recombinant plasmid was successfully cloned into eukaryotic expressing vector,and the expression of pEBG-hPAK4 fusion protein was identified and pulled down by Glutathione Sepharose 4B.