Network resources can exactly make up for it. Making good use of the search engine can expand extracurricular professional English learning for students;provide teachers with lesson plan-ning material and learners with the correct pronunciation of the vocabulary. Network digital repository offer students learning platform and PBL teaching in the QQ group can be tried. Electronic dictionary software helps to read the professional literature and E-mail is a convenient means of communication. These help to create language environment and enhance bilingual teaching effect.

Objective To summarize the nursing experience for three cases of above 1-month-oldinfants with total transposition of the great artery after Switch operation.Methods The vital signs of the infants were monitored,including the respiratory system,circulation system,temperature,urine quantity,feeding and delayed sternal closure.Appropriate measures were adopted to cope with changes in the above mentioned aspects.Results All the 3 infants were cured with good nursing effect.Conclusion Switch operation was a complicated operation.The 3 infants missed the-optimum operation time and left great difficulty for nursing.Close monitoring and delicate nursing proved to be the pivotal process to ensure the successful curement.

Objective To investigate the role of endogenous histamine in Parkinson disease. Methods 6-OHDA-lesioned rats were prepared by the conventional mothod,and in the meantime a group of rats were administrated with ?-fluoromethylhistidine(?-FMH),an irreversible inhibitor of histidine decarboxylase(HDC),via intracerebroventricular injection(12.5 ?g or 25 ?g,i.c.v.) for seven days.On the 7 day,the apomorphine-induced turning behavior and were detected, and the immunoreactivity of dopaminergic neurons in the substantia nigra pars compact(SNc) and histaminergic neurons in the tuberomammillary nucleus(TMN) were evaluated by tyrosine hydroxylase(TH) and HDC immunohistochemistry,respectively.Additionally,the level of dopamine in striatum was determined with high performance liquid chromatography(HPLC). Results ?-FMH(25 ?g,i.c.v.) significantly reduced the turning behavior and prevented the loss of dopaminergic neurons in the SNc,and slightly increased dopamine level in the striatum.Whereas,the immunoreactivity of histaminergic neurons in the TMN of hypothalamus in both the 6-OHDA lesioned and the ?-FMH treated rats was not changed.Conclusion Endogeneous histamine may involve in the pathological processes of PD.However,the histaminergic neurons are not involved in PD.

OBJECTIVE:To observe the effects of epidural anesthesia analgesia on childbearing. METHODS:197 delivery woman were randomly divided into observation group (95 cases) and control group (102 cases). Control group was given natural childbirth,and observation group was additionally given epidural anesthesia analgesia. Neonatal Apgar score (5 min),behavioral neurological score and VAS,mother-infant situation,the incidence of ADR were compared between 2 groups. RESULTS:There was no statistical significance in neonatal Apgar score and behavioral neurological score between 2 groups (P>0.05);VAS score and the rate of cesarean section in observation group was significantly lower than in control group,with statistical significance(P<0.05). There was no statistical significance in the incidence of postpartum hemorrhage,vaginal midwifery and asphyxia neonatorum (P>0.05). ADR as itchy skin,nausea and vomiting,headache,limb numbness of observation group were improved after symptomat-ic treatment. CONCLUSIONS:The epidural anesthesia analgesia have no effect on childbearing and can relieve labor pain with good safety.

Objective To evaluate the valuse of endoscopic ultrasonography ( EUS ) for upper gastrointestinal submucosal lesions and the role of Photoshop in differentiating leiomyoma and stromal tumors. Methods Data of 656 patients with upper gastrointestinal submucosal lesions evaluated by EUS and receiving endoscopic submucosal dissection ( ESD ) from April 2010 to March 2015 were collected and retrospectively analyzed. The goldern standard for lesions size and origin was the intraoperative diagnosis of ESD, and that of the type of lesions ( leiomyoma, stromal tumor, ectopic pancreas, lipoma, etc.) was pathological and immunohistochemical finding. The consistency of diagnosis of the EUS was evaluated. In addition, Photoshop was used to differentiate diagnosis of leiomyoma and stromal tumors which were confirmed by pathology and immunohistochemistry. Results The consistency in diagnosing the size and lesion origins was 92?56%(560/605) and 88?43%(535/605)between EUS and ESD intraoperative result. The consistency in pathological types of EUS was 79?34%(480/605). Photoshop was used to analyze the EUS images of 177 stromal tumor and 241 leiomyoma. The gray value of stromal tumor was significantly higher than that of leiomyoma(59?97 VS 39?39, t=43?27, P<0?05).The echo of stromal tumor was higher. The standard deviation of gray value of leiomyoma were significantly lower than that of the stromal tumor ( 4?81 VS 5?42, t = 2?83, P < 0?05 ) , indicating the echo uniformity of leiomyoma was better. Conclusion EUS shows high accuracy rate for upper gastrointestinal submucosal lesions, providing more accurate basis for endoscopic therapy. EUS combined with Photoshop is helpful for differentiating stromal tumor from leiomyoma.

Objective To compare changes in dental arch and soft and hard tissue produced by the Damon Q self-ligating bracket and 3M metal bracket with non-extraction treatment in maxillary mild-to-moderate crowding cases. Meth-ods Eighty seven patients were selected in our study, who were assigned to 4 groups by the crowding degree of maxillary and appliance type:ⅠandⅡcrowding degree using Damon Q self-ligating bracket groups (41 cases);ⅠandⅡcrowding de-gree 3M metal bracket groups (46 cases). We measured lateral cephalometric radiographs and dental casts at both beginning and end of treatment. Paired t-tests and group t-tests were used in statistical analysis to compare the alteration in dental arch and soft and hard tissue. Results There was significant increase in width and length of maxillary dental arch by non-extraction treatment withⅠandⅡcrowding degree maxillary, but the results of both groups were not of statistical difference. To compare hard and soft tissue in two groups withⅡcrowding degree maxillary, the reduction of nasolabial angle, angle of inclination of upper lip, angle of upper lip and lower lip had obvious statistical significance and it is more prominent in self-ligating bracket group than in conventional bracket group. Conclusion Using non-extraction treatment, when maxillary crowding is moderate, ,Damon Q self-ligation bracket group result in more prominent changes of upper lip protrusion than 3M bracket with more marked crowding . Therefore, clinical doctors should pay more attention to choose bracket and treat-ment system for patients with marked crowding of maxillary dentition.

Objective To analyze the renal IgG subclasses in special patients whose renal HBsAg and HBcAg are positive, but plasma HBsAg, HBeAg, and HBcAg are negative.Methods Renal IgG subclasses were compared between 14 cases hepatitis-related nephropathy patients(diagnosed by renal biopsy pathology,whose blood hepatitis B antigens were negative) and 18 cases idiopathic membranous nephropathy patients.HBcAg and HBsAg were detected by indirect immunofluorescence assay, IgG, IgG1, 2, 3, 4 were stained by immunofluorescence.Results Renal IgG1-4 deposits were 100％ (14/14), 78.6％ (11/14), 78.6％ (11/14), 100％(14/14) separately in hepatitis B-related nephropathy group, and renal IgG1-4 deposits were 88.9％ (16/18), 5.6％ (1/18), 5.6％ (1/18), and 83.3％ (15/18) in idiopathic membranous nephropathy group.Renal IgG2 and IgG3 deposit more in hepatitis B-related nephropathy group than in idiopathic membranous nephropathy group (78.6％ vs 5.6％ ,78.6％ vs 5.6％;P =0.000) , but no significant difference in IgG1 and IgG4 deposit.Conclusion Renal IgG2 and IgG3 deposit more in hepatitis B-related nephropathy group than in idiopathic membranous nephropathy group, and may help some in diagnosis.

OBJECTIVE:To optimize the extraction technology for organic acids in Crataegi fructus. METHODS:Based on sin-gle factor test,using liquid-solid ratio,extraction time and ethanol volume fraction as independent variables,extraction yield of or-ganic acids as dependent variable,central composite design-response surface method was used to optimize the extraction technology of organic acids in Crataegi fructus. RESULTS:The optimal extraction technology was as follow as liquid-solid ratio of 18.5:1, adding 75% ethanol,reflux extraction twice,2.0 h each time. Average extraction yield of organic acids in verification test was 5.22%(RSD=2.70%,n=3),with 1.75% relative error of the predicted value(5.13%). CONCLUSIONS:Optimized extraction technology for organic acids in Crataegi fructus is simple,with good reproducibility and predictability.

Objective To establish a nucleic acid testing assay for bacterial detection with meeting automatic requirement,and use it preliminarily to detect bacterial contamination in concentrated platelet.Methods Optimizing the bacterial DNA extraction methods,evaluating the sensitivity and specificity of the optimized assay with different bacterial strains,and applying the assay on bacterial detection for 100 units of concentrated platelet.Results The optimized extraction methods met the criteria of automatic testing.The sensitivity of the assay is 10,150,65,15 CFU/mL for Escheriehia coli,Stphylococcus aureus,Bacillus subtilis,and Pseudomonas aeruginas respectively.There was no non-specific amplification detected.And the assay could detect 1 contaminated bacterial from 100 units of concentrated platelet as same as BactALT bacterial culture.Conclusions Nucleic acid testing for bacterial genome 16S DNA could detect common contaminated bacterial sensitively and fast from platelet product.This assay could have the same effect when compared to bacterial culture methods when it was applied in concentrated platelet products.

Objective:To understand the incidence of sleep disorder in human immunodeficiency virus/acquired immune deficiency syndrome (HIV/AIDS) patients before antiviral therapy, and to explore its risk factors.Methods:200 newly treated HIV/AIDS patients who visited the Eighth Affiliated City Hospital of Guangzhou Medical University from January to June 2016 were randomly selected. According to the Pittsburgh Sleep Quality Index (PSQI), they were divided into a good sleep group and a Sleep disorder group; The influencing factors of sleep disorder in HIV/AIDS patients were analyzed by univariate analysis and multivariate logistic regression.Results:The incidence of Sleep disorder in 200 HIV/AIDS patients before antiviral therapy was 22.5%(45/200); CD4 + T cell count was (414.13±202.16)/μl; 29%(58/200) of patients had CD4 + T cell counts<200/μl. There were significant differences in CD4 + T cell count and the proportion of patients with syphilis infection, comorbidity anxiety and comorbidity depression between the good sleep group and the Sleep disorder group (all P<0.05). Multivariate logistic regression analysis showed that syphilis infection ( OR=4.606; 95% CI: 1.973-10.752; P<0.001), comorbidity anxiety ( OR=2.496; 95% CI: 1.086-5.737; P=0.031) and comorbidity depression ( OR=2.087; 95% CI: 0.915-4.760; P=0.040) were risk factors for sleep disorder in HIV/AIDS patients before antiviral treatment. Conclusions:The incidence of Sleep disorder in HIV/AIDS patients before antiviral therapy in Guangzhou is high, especially in patients with syphilis infection, comorbidity anxiety and comorbidity depression. The sleep disorder of HIV/AIDS patients should be assessed and detected early, and multiple interventions should be taken to improve sleep quality.