With the continuous development of gene therapy, more new technologies have been found and gradually applied in the basic research and clinical treatment of various diseases. In the field of AIDS treatment, gene therapy shows obvious advantages over traditional antiretroviral therapy. In recent years, short hairpin RNA (shRNA), clustered regulatory interspaced short palindrome repeat (CRISPR), transcription activator-like effector nucleases (TALEN), and zinc finger nucleases (ZFN) have been applied in AIDS treatment and have fruitful results. This paper reviews the major breakthroughs in this field since the emergence of gene therapy and the latest research achievements and their progress, with a view to providing a useful reference on a wider range of gene therapy application.

Objective To explore the relationship between serum sialic acid(SA),high-sensitivity C-reactive protein(hs-CRP) and prehypertension and their clinical significance.Methods According to the blood pressure range,160 residents who received physical examination were divided into three groups:normal group (40 cases),prehypertension group (60 cases) and hypertension group (60 cases).The blood pressure,height and weight were measured in all subjects,and the serum levels of SA,hs-CRP,serum total cholesterol (TC),low density lipoprotein (LDL),high density lipoprotein(HDL),triacylglycerol(TG) and fasting plasma glucose(FBG) were detected on the same day.Multiple linear regression was used to investigate the relationship between serum SA and hs-CRP levels and systolic blood pressure(SBP) and diastolic blood pressure(DBP).Results The levels of DBP,SBP,body mass index(BMI),SA,hs-CRP,TC,LDL,HDL and FBG among the three groups were statistically significant (F =1 102.039,2 690.642,9.163,116.511,103.634,5.431,4.267,67.352,4.236,all P ＜ 0.05).The levels of BMI,SA and hs-CRP increased gradually with the increase of blood pressure,while the level of HDL decreased (P ＜ 0.05).The levels of TC and LDL in the hypertension group were higher than those in the normal blood pressure group,but there were no statistically significant differences in TC and LDL levels between the pre hypertension group and normal blood pressure group (all P ＞ 0.05).Multiple linear regression showed that the levels of BMI,SA,hs-CRP and FBG were positively correlated with SBP(r =3.66,2.61,3.24,2.39,all P ＜ 0.05),and the levels of BMI,SA,hs-CRP and LDL were positively correlated with DBP (r =2.20,2.34,3.48,2.28,P all ＜ 0.05).Conclusion The levels of serum SA and hs-CRP are positively correlated with blood pressure,which can be used as assessment criteria for prehypertension.

Objective To investigate the efficacy and cardiovascular disease of Danhong injection in the treatment after cardiac interventional therapy.Methods A total of 64 cases with cardiac intervention from September 2013 to July 2015 in our hospital were selected and divided into control group and experiment group with 32 cases in each group.Patients in the control group were given aspirin 100mg orally,and patients in the experiment group revieved Danhong injection 20mL intravenous drip qd,one times a day for 14 days.Two groups of patients underwent cardiac intervention.After the operaiton,the control group was given aspirin enteric-coated tablets 100mg orally,clopidogrel 75mg orally,and patients in the experiment group revieved Danhong injection 20mL intravenous drip qd,seven days for a course and the patients treated for three courses.The clinical efficacy,C-reactive protein(CRP),cardiac troponin T(TnT),P-selectin(Ps),PSGL-1,cardiovascular events(cardiovascular events),and adverse events were compared.Results After treatment,the clinical efficacy in experiment group was 93.75％,which significant higher than that in control group 71.88％,the difference was statistically significant(P<0.05).The rate of cardiovascular events in experiment group was 21.88％,which significant lower than that in control group 40.63％,the difference was statistically significant(P<0.05).The serum C-reactive protein,troponin T,Ps and PSGL-1 were significantly decreased in the two groups,and the experimental group was significantly smaller than the control group,the difference was statistically significant(P<0.05).The incidence of adverse reactions was 15.63％in the control group and 9.38％in the experimental group,the difference was not statistically significant.Conclusion Danhong injection treatment of elderly patients with cardiac intervention after the clinical effect is significant,high security,the incidence of cardiovascular events is low.

Objective To explore the establishment and application of scientific research personnel performance evaluation system based on Research Resource Planning (RRP).Methods Establish the index system and weight coefficient of scientific research personnel performance evaluation system by Delphi Method and Analytic Hierarchy Process.The evaluation system is applying for tailored scientific research personnel training according to the analysis of current development status of young researchers.Results Scientific research personnel performance evaluation system,based on the indicators of research capacity,research outcome and research platform is established and applying for personnel training,personnel assessment and incentive policy-making.Conclusions The evaluation system based on RRP can objectively reflect the current situation of the development of scientific research personnel,which plays an important role in promoting the sustainable development of scientific research personnel.

Objective To provide helpful experiences for the development,construction,and evaluation of RRP through constructing the evaluation system.Methods The study of literature and comprebensive analysis were employed.Results Putting forward the principle of establishing the evaluation system and finally,attempting to build an evaluation system that specifically aiming at the RRP system.Conclusions The evaluation methods should be updated.

Objective To evaluate the research performance based on DEA and to provide the corresponding development measures for medical subjects in hospital.Methods Based on the analysis of medical subjects at Peking University People's Hospital,this paper explored the evaluation index system of medical subjects.The output-oriented DEA was selected and applied in the performance evaluation.Results We figure out that the DEA ratio of every medical subject with the established method in the program of DEAP Version 2.1.Conclusions The method based on DEA can reflect the performance of medical subjects,and the analysis of the evaluation results can provide the management department of hospital with evidences and references for policy making of medical subjects' development.

Objective To investigate the difference of apparent diffusion coefficients (ADCs) changes in three major salivary glands after gustatory stimulation using two different stimuli. Methods Thirty healthy volunteers were examined with a 1.5 T MR unit. A diffusion-weighted MR imaging (MR DWI) sequence was performed once at rest and continuously repeated 13 times after gustatory stimulation using a commercially available lemon juice and vitamin C tablets in the same volunteer by using self-controlled method. The subsequence of two stimuli was random. In addition, the salivary flow rates at rest and after stimulation were measured. Characteristics and differences in ADCs curves of three salivary glands before and after stimulation between two stimuli were analyzed. Comparison of maximum ADCs, maximum ADCs increase rates (IRs) and times to maximum ADCs(Tmax) between two stimuli was performed by using independent-samples t test. Correlation analysis between rest salivary flow rates and rest ADCs, the maximum salivary flow rates and ADCs after stimulation, the maximum salivary flow IRs and ADC IRs after stimulation were performed by using Pearson correlation test. Results In lemon juice stimulation group, the mean ADCs mostly showed a steady increase to peak values during the first DW MRI scan after stimulation in all glands, followed by a gradually decrease fluctuating slightly around the baseline values. In vitamin C stimulation group, the mean ADCs were significantly increased in all glands during the first DW MRI scan after stimulation, followed by a gradual upward trend till peak values. In lemon juice stimulation group, the mean Tmax of submandibular and sublingual glands[(184±122)s, (345±232)s, respectively] were significantly earlier than those[(454 ± 301)s, (528 ± 297)s, respectively] in vitamin C stimulation group (t=-3.517 and-2.548 respectively, P<0.01 for all). The mean maximum ADCs of three glands in lemon juice stimulation group[(1.05 ± 0.12) × 10-3 mm2/s, (1.22 ± 0.10) × 10-3 mm2/s and (1.26 ± 0.21) × 10-3 mm2/s, respectively] were all lower than those in vitamin C stimulation group[(1.13±0.13) ×10-3 mm2/s, (1.32±0.25) × 10-3 mm2/s and (1.57 ± 0.36) × 10-3 mm2/s, respectively], and the differences in parotid and sublingual glands between two groups were significant(t=-2.894 and-3.681 respectively, P<0.01 for all). The mean maximum ADC IRs of three glands in lemon juice stimulation group[(11.35±4.07)%, (8.81±5.40)%, (34.08±21.66)%, respectively] were significantly lower than those[(17.80 ± 12.72)%, (18.16 ± 18.93)%, (67.49 ± 46.04)% , respectively] in vitamin C stimulation group (t=-2.252,-2.330 and-3.432 respectively, P<0.05 for all) . In two groups, the mean maximum ADC IRs of parotid and submandibular gland were all significantly lower than sublingual gland (t=-5.994 and-6.443 respectively, P<0.01 for all). No correlation was observed between ADCs and salivary flow rates, ADC IRs and salivary flow rate IRs in two groups (P>0.05). Conclusion MR DWI with transient stimulation using lemon juice is more stable for evaluating the physiologic changes of salivary glands in vivo.

Based on the analysis of the status quo and problems of the management of companyfunded research projects,the feasible management measures are proposed and a standardized management mode for company-funded research projects is established.The establishment and application of the management mode are effective in regulating the supervision and management of those projects,facilitate the implementation of the projects,and optimize the use of research funds.

Objective To summarize the hospital research and development fund achievements in 5 years,continuing to promote innovative management concept.Methods By summarizing the fund's input and output of scientific research during 2006 to 2010,analysis of the effects in the cultivation of medical scientific research personnel and promotion of discipline development in hospital after the implementation of innovative management ideas.Results After the implementation of the innovation process management,output of fund is well.It has played a positive role in promoting to the scientific research personnel and discipline development.Conclusion It should be further promoted of the innovation process management idea,in order to further play the role of seed fund role in talent,discipline development.

It is key for research institutions to ensure the standardized supervision of the progression and fund use of research projects.Peking University People's Hospital designed and applied a whole-process management mode of projects based on information system,which oversaw the conduct and funds management of projects.The application of this mode has significantly promoted the management level of scientific research projects.