1.Occurrence of Ureaplasma parvum and Ureaplasma urealyticum in Women with Cervical Dysplasia in Katowice, Poland.
Alicja M EKIEL ; Daniela A FRIEDEK ; Malgorzata K ROMANIK ; Jaroslaw JOZWIAK ; Gayane MARTIROSIAN
Journal of Korean Medical Science 2009;24(6):1177-1181
The aim of this study was to evaluate the occurrence of genital mycoplasmas, especially Ureaplasma parvum and Ureaplasma urealyticum, in women with atypical squamous cells of undetermined significance (ASCUS), low grade squamous intraepithelial lesions (LSIL) and high grade squamous intraepithelial lesions (HSIL), compared to women with normal cytology living in Katowice, Poland. Two sterile swabs were used to obtain material from the posterior vaginal fornix of 143 women with squamous intraepithelial lesions and 39 healthy women: first for general bacteriology, second for detection of urogenital mycoplasmas using Mycoplasma IST2 kit. From each positive Mycoplasma IST2 culture DNA was isolated and PCR was performed for identification of U. parvum and U. urealyticum. Mycoplasma IST was positive in 34.1% cases. Urogenital mycoplasmas were demonstrated in women with HSIL significantly more often compared to women with LSIL, ASCUS, and with normal cytology. DNA of U. parvum was demonstrated in majority of Mycoplasma IST2-positive cases, U. urealyticum DNA-only in 9 (4.9%). Predominance of 3/14 serovars of U. parvum was demonstrated. U. urealyticum biovar 2 was present more often in women with squamous intraepithelial lesions.
Adult
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Animals
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Female
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Humans
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Male
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Poland/epidemiology
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Pregnancy
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Ureaplasma/*genetics
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Ureaplasma Infections/epidemiology/*microbiology
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Ureaplasma urealyticum/*genetics
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Uterine Cervical Dysplasia/*microbiology/pathology
2.Impact of Chlamydia trachomatis and HPV Infection Among Sexually Active Teenage Girls in Upper Silesia, Poland.
Daniela FRIEDEK ; Alicja EKIEL ; Malgorzata ROMANIK ; Zbigniew CHELMICKI ; Artur CHELMICKI ; Gayane MARTIROSIAN
Journal of Korean Medical Science 2005;20(4):704-705
No abstract available.
Adolescent
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Adult
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Antigens, Bacterial/analysis
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Chlamydia Infections/*diagnosis/epidemiology/microbiology
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Chlamydia trachomatis/immunology
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DNA, Viral/analysis
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Female
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Humans
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Mass Screening/*methods
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Papillomavirus Infections/*diagnosis/epidemiology/virology
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Papillomavirus, Human/genetics
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Poland/epidemiology
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Prevalence
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*Sexual Behavior
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Urban Population