2.Clinical Observation on Treatment of 460 Community-acquired Pneumonia Cases in Children with Qing-Fei Tong-Luo Ointment
Xiuying ZHANG ; Xuefeng WANG ; Yunxiao SHANG ; Zhenze CUI ; Yan HUANG ; Yongsheng WANG ; Yingchun LI ; Tong FENG ; Yanqi SU ; Fengli YU ; Ling ZHANG ; Xueying JIN ; Aimin SUN ; Yixiang LIU ; Danhua SHI ; Han ZHANG ; Zhenqi WU ; Yue ZHAO
World Science and Technology-Modernization of Traditional Chinese Medicine 2017;19(12):2054-2057
This study was aimed to evaluate the clinical effect and safety of Qing-Fei Tong-Luo (QFTL) ointment for treating children with pneumonia.Randomized controlled trial (RCT) was conducted among 460 cases of children with pneumonia.The observation group was given QFTL ointment combined with basic treatment.And the control group was only treated by basic treatment.Evaluation was given on the total clinical efficacy,disappeared time of fever,cough,expectoration,shortness of breath,and medication safety.The incidence of respiratory diseases was followed up on the 30th days after drug withdrawal.The results showed that in the aspect of clinical efficacy between two groups,the cure rate of the observation group was 98.26%,and that of the control group was 93.89%,with statistic significance (P < 0.05).The cure rate of the observation group was better than that of the control group.There was statistical difference on expectoration disappeared time (P < 0.05).There was no statistical difference on disappeared time of fever,cough and shortness of breath (P > 0.05).There was statistical difference on the incidence of respiratory diseases on the 30th days followed-up after drug withdrawal (P < 0.05).There was no statistical difference on the incidence of upper respiratory tract infection,pneumonia and asthma (P > 0.05).No adverse reactions occurred in the observation group.It was concluded that QFTL ointment combined with basic therapy on the treatment of pneumonia in children was significantly better than the control group in the aspect of clinical efficacy,expectoration disappeared time and the incidence of bronchitis.It is safe and effective.The prognosis is good and worthy of promotion in the clinical practice.
3. Construction, characterization and stability analysis of infectious clone of live attenuated dengue virus type 4 Ban18HK20 strain
Enyue FANG ; Ling WANG ; Danhua ZHAO ; Ming LI ; Minglei LIU ; Yuhua LI
Chinese Journal of Microbiology and Immunology 2019;39(11):827-834
Objective:
To construct a stable infectious clone of live attenuated dengue virus (DENV) type 4 Ban18HK20 strain for better understanding the virulence determinants of DENV and improving the development of chimeric vaccines.
Methods:
Specific primers were constructed according to the genome of Ban18HK20 strain and used to subclone six cDNA fragments, which were linked into a high-copy plasmid pSPTM to obtain a stable full-length cDNA clone of DENV. RNA was transcribed from the full-length cDNA