1.Defining the Scope of Chronic Non-communicable Diseases under the Perspective of the Global Disease Burden
Dangdang LEI ; Hua YANG ; Mingxia JING
Chinese Health Economics 2014;(7):21-23
Based on the global burden of disease classification, to explore the scope of chronic diseases. Methods:Searching relevant literature and books to screen and remove the acute non-communicable diseases among the non-communicable diseases which is the second category of global burden of disease ( GBD) classification. Results: Acute non-communicable diseases mainly concentrated on the acute inflammatory diseases, it also existed in other acute diseases which are not caused by infection. Conclusion: To screen and remove the acute non-communicable diseases in the non-communicable diseases could define the scope of non-communicable chronic diseases on certain extent, which has certain significance for the workers who study all chronic diseases.
2.Effect of pleiotrophin on decidualization of uterine stromal cells in mice
Haifan YU ; Chuanhui GUO ; Dangdang LI ; Hongliang ZHANG ; Shuang GENG ; Zhanqing YANG ; Bin GUO ; Zhanpeng YUE
Journal of Jilin University(Medicine Edition) 2016;42(5):855-859
Objective:To construct the pleiotrophin (PTN )overexpression vector,and to explore the effect of PTN on the decidualization of uterine stromal cells in the mice.Methods:The specific primers containing restriction enzyme cutting sites were designed according to the PTN gene sequences published in GenBank for PCR amplification.The amplified fragment of PTN was recovered from the agarose gel and cloned into the pGEM-T vector.The pGEMT-PTN was cut by double enzyme digestion and ligated into pcDNA3.1 (+)to construct the PTN overexpression plasmid.After transfection with PTN overexpression plasmid,the expression levels of PTN mRNA in the uterine stromal cells and the expression levels of decidualization markers Prl8a2 and Prl3c1 were detected by qRT-PCR method.The uterine stromal cells transfected with pcDNA3.1 (+)empty vector were used as control group. Results:The results of identification by double enzyme digestion indicated that the bands of PTN overexpression plasmid were consistent with those of the target gene,and the clone sequencing results suggested that it had 100% homology with mouse PTN gene sequence published in GenBank.Compared with control group, the expression levels of PTN,Prl8a2 and Prl3c1 mRNA in mouse uterine stromal cells in PTN overexpression group were significantly increased (P < 0.05).Conclusion:PTN overexpression could increase the expression levels of decidualization markers in mouse uterine stromal cells,indicating that PTN might play an enhancement effect during uterine decidualization in the mice.
3. Progress of Research on Relationship Between Intestinal Flora, Immunity and Gallstone
Yaqian NING ; Huili WANG ; Dangdang YANG ; Shan LIN ; Bing HU ; Yaqian NING ; Wenhao QIN ; Huili WANG ; Dangdang YANG ; Shan LIN ; Bing HU
Chinese Journal of Gastroenterology 2023;28(5):302-306
Gallstone disease is a common and frequently seen disease of digestive system, and is a common cause of inflammation of gallbladder and bile duct, biliary tract tumor and obstructive jaundice, however, its pathogenesis is not yet fully understood. Recent studies have revealed that intestinal flora and immunity play important roles in the formation of gallstones. This paper reviewed the relationship between the abundance and composition of intestinal flora, immuno - globulins, mucins and immune cells, as well as the interactions between intestinal flora and biliary immunity and gallstone formation for providing new ideas and methods for gallstone prevention and treatment.