Background: In 2006, Center for research and production of vaccines and biologicals \ufffd?Ha Noi produced 10 million doses of poliomyelitis vaccine met quality standard which given by World Health Organization (WHO). The monkeys for vaccine production were selected and monkey kidney cells were tested arcording to the WHO requirements (the strong moykeys with no adventitous agents). Objectives: To assess the rates of mokeys (at the Reu island in Quang Ninh province) with no adventitous agent infection and the cells using to produce type 1 poliomyelitis vaccine in 2006. Subjects and method: The study was performed by using microneutralization technique on 39 Macaca mulatta mokeys with weights were 1.5 to 2.5 kg. Results: 11 out of 39 monkeys (28,21%) are negative to SIV, SV40, poliomyelitis viruses, foamy virus as well as tuberculin test. 100% lots of primary monkey kidney cells culture produced in 2006 are free from adventitous agents arcording to the WHO requirements. Conclusion: Center for research and production of vaccines and biologicals - Ha Noi implemented strictly under the WHO guidelines on selection of monkeys and monkey kidney cells for OPV production from Sabin strains.
Poliovirus Vaccine ; Oral ; Macaca mulatta ; Haplorhini

Background: Trivalent poliomyelitis vaccine was produced from strains which had been supported by Japan. One of the standards of vaccine quality required by WHO is potency and thermostability. Follow that potency at 370C and within 48 hours is not less effective than potency at -200C exceedingly 0,5 lgCCID50. Objectives: To assess the potency of Trivalent poliomyelitis vaccine and the thermostability of mokey vaccine preserved at 370C within 48 hours. Subjects and method: 12 lots of trivalent poliomyelitis vaccine (included 3 types) produced in January 2007 were evaluated by microneutralization technique. Results: Potency of 12 type I lots were all 106,0 CCID50/0,1ml and the disparity of potency at two temperatures were all < 0,5 lgCCID50/0,1ml. Potency of 12 type II lots were all 105,0 CCID50/0,1ml and the disparity of potency at two temperatures were all < 0,5 lgCCID50/0,1ml. Potency of 12 type III lots were all 105,5 CCID50/0,1ml and the disparity of potency at two temperatures were all < 0,5 lgCCID50/0,1ml. Conclusion: 12 final poliovaccine lots produced in Center for research and production of vaccines and biologicals \ufffd?Ha Noi in 2007 met WHO requirements for potency and thermostability.
Poliomyelitis ; Nonpoliovirus ; Poliovirus Vaccines/ therapeutic use ; Oliomyelitis/ immunology ; epidemiology

Background: \r\n', u'Chicken embryo cell was the most effective fibroblast cell line for producing the attenuated measles vaccines. Many countries as Japan, in Kitasato, an attenuated virus derived from the Edmonston virulent strain adapted in chick embryo cells, it was call AIK- C strain, and used for measles vaccine production. In Vietnam, the POLYVAC have applied the Kitasato institute\u2019s technology into measles vaccines production. The Valo SPF egg (Lohmann-Germany) was used in the chick embryo culture \r\n', u'Objectives: \r\n', u'To study the inoculation, and clone of the human AIK-C strain in the chick embryo in order to achieve a high yield of virus for measles vaccine production \r\n', u'Subjects and method:\r\n', u'Suitablecells (AIK- C Strain, an attenuated virus derived from the Edmonston virulent strain, chicken embryo cell cultured from SPF egg Valo Germany) are selected. Then methods as inoculating and culturinglive viruses on the chicken embryo cell are applied.\r\n', u'Results:\r\n', u'After 9 days for infection, viruses which were observed via microscope, propagated and in infectious clone by the measles virus.\r\n', u'Conclusion: \r\n', u'Many tests for propagation of AIK measles virus in chicken embryo cell cuture derived from Valo SPF egg (Germany) showed that chicken embryo cell culture from Valo SPF egg (Germany) is appropriated fro AIK- C measles vaccine production in Vietnam.\r\n', u'
Measles virus ;

Background: Rota vaccine is used to prevent diarrhea in children under 5 years old. Two vaccines are being used in developed countries: Rotarix (GSK) and RotaTeq (Merk). Rotarix vaccine was produced from master seed G1P8 and RotaTeq vaccine was from the coordination of human rotavirus strains G1, G2, G3, G4 and cow rotavirus strain.Thanks to the helps of WHO, Ministry of Health and Ministry of Science and Technology, Center for research and production of vaccines and biologicals \ufffd?Ha Noi made study of creating rotavirus master seed G1P4 for Rota vaccine production in Vietnam. Objectives: To evaluate the safety and potency of rotavirus master seed G1P4 in the laboratory and experimental animals. Subjects and method: Rotavirus master seed G1P4 (2001019210) lot 1 (MS-P5) and lot 2 (MS-P5) produced in 2005, preserved at -800C were determined potency by Immunofluorescence (IF) method and tested for safety on rabits and rats. Results:2 lots of Rotavirus master seed G1P4 that had been produced in Center for research and production of vaccines and biologicals \ufffd?Ha Noi had high titre and safety in the laboratory and experimental animals. Conclusion: The result was the basis of Rota vaccine production in Vietnam.
Rotavirus/ diagnostic use ; Rotavirus Vaccines/ isolation & ; purification ; contraindications

A lectin from the haemolymph clam was purified by molecular sieving on sephadex- G75 column. The products have a high purity. The purity of lectin was assessed by SDS polyacrylamide gel. Two doublets band of apparent molecular weights of respectively about 14,000-17,000 and 20,000-23,000 Dalton were revealed upon staining with Coomassie blue. Optimum-pH of the lectin from the clams is pH 6,5-7,0 and 8,5-9,0. The lectins of the clams had very different specificity for hydratecarbons and glucoproteins
isolation & purification ; Lectins ; medicine

External compression was a simple, safe and effective method with less complication to treat the medical and giant omphalocele, especially in the lack of artificial transplant or method of abdominal wall reconstruction with complication.
Hernia, Umbilical ; Abdominal Wall ; therapeutics

The magnetic nearly do not have any disadvantages at therapeutic doses and do not cause the changes of cell structure and heteroplasia. The dependence on the magnetic is rare so the treatment with magnetic can be lasted. However, it has not known the side effects of the magnetic after many years (15-20) of the application of magnetic because the magnetic was applied newly
Magnetics ; Delivery of Health Care

A total of 220 pregnant women were given iron-folic acid tablets within 3 months. The initial nutritional anemia rate of 90% was decreased to 75,90% after the administration of supplementation
Pregnant Women ; Dietary Supplements

Trypsinization method in our modification was harvested high yields of dissociated cells. The total of cells is obtained 19.47 X 108 per pair of monkey kidney weighted 10 gram. Whereas rate of available cell reach 83.9% and rate of single cell and small group (<5 cells) collected was about 90%.
Trypsin ; Haplorhini ; Kidney ; Cells

During 1993-1997, the rate of expanded program of immunization for 6 infectious diseases prevention for children under 1 year in Thanh Hoa province reached 95% annually. Tetanus preventive immunization rate for pregnant women was 93%, for women from 15-35 years accounted for 93% for the past 5 years the morbidity and mortality rate caused by 6 infectious diseases in children were significantly reduced
Immunization ; Communicable Diseases ; Infant