Objective To build rat models and observe changes of behavior and monoamine of PDD. Methods The rats were injected with 6-OHDA and accepted CUMS for the establishment of PDD model , so as to assess its behavior changes and detect brain tissue monoamine contents by ELISA. Results Significant behavioral and monoamine abnormalities can be observed in model rats. Behavioral and monoamine improvement can be observed after treated with Chaigan Jieyou. Conclusion The PDD model can be established by 6-OHDA and CUMS. Chaigan Jieyou decoction may reach therapeutic purposes by increasing monoamine contents.

Objective To study the clinical application of laparoscopy combined with hysteroscopy to female infertility.Methods From January 2007 to March 2008,145 cases of female infertility were examined and treated by laparoscopy combined with hysteroscopy in our hospital.We analyzed the clinical records of the patients to determine the value of the method.Results By using laparoscopy combined with hysteroscopy,136 cases of pelvic disease(93.8%,136/145) were detected in this series.The most common causes of infertility were pelvic inflammation(58.6%,85/145) and endometriosis(29.0%,42/145).The incidence rate of pelvic inflammation in secondary infertility patients was significantly higher than in those with primary infertility(P

Objective Comparing with traditional teaching methods of oral histopathology, to probe whether the interactive teaching system of the digital microscope can improve the teaching effi-ciency of experiment of oral histopathology courses. Methods The subjects were the 2010 grade students (40 students) and the 2011 grade students (38 students) majoring in stomatology of South Medical University, and they were evenly divided into 2 groups at random. In the first part of the ex-periment of Grade 2010, the teaching of traditional optical microscope was used in a group of experi-mental courses, and interactive teaching with digital microscope was used in the other group. In the second part of the experiment the two groups swapped the teaching method. While in the whole exper-iment of Grade 2011, traditional optical microscopes were adopted in one group, and in contrast, the interactive teaching system of the digital microscope was adopted in the other group. The P values of the 5 indexes were less than 0.05. After the courses, the 2010 grade students received the questionnaire survey and the 2011 grade students received the quiz about the experimental course, and then the evaluation results of the two groups were compared for t inspection, using SPSS software t test. Result The questionnaire results of Grade 2010 showed that the scores of digital microscope interactive group were higher than those of traditional teaching group in satisfaction with teaching , teacher-student interaction, classroom atmosphere active degree, learning interest, and the understanding of the teach-ing content. The experiment course content test results of Grade 2011 showed that the digital interactive group students averaged 46.26, while the average score of the traditional teaching group was 42.78%. The difference between the two groups had statistical significance, P=0.001. Conclusion Compared with the traditional teaching methods, the interactive teaching system of the digital microscope can enhance the academic grades, improve the teaching efficiency of experiment of oral histopathology courses, and elevate the teaching quality.

Objective-To-investigate-the-feasibility-of-total-laparoscopic-hysterectomy-for-complicated-hysteromyoma-,-such-as-broad-ligament-myoma,-lower-uterine-fibroids,-or-cervical-myoma.-Methods-From-December-2009-to-December-2012,-we-performed-laparoscopic-hysterectomy-on-166-patients-with-complicated-hysteromyoma-and-on-170-patients-with-common-hysteromyoma-.-The-operation-time-,-intraoperative-blood-loss-,-recovery-time-of-gastrointestinal-function-,-postoperative-morbidity-,-and-the-rate-of-conversion-to-laparotomy-were-compared-between-the-two-groups-respectively-.-Results-No-significant-differences-were-detected-in-operation-time-[(112.2-±15.3)-min-vs.(110.3-±16.1)-min,-t=1.088,-P=0.277],-intraoperative-blood-loss-[(117.0-±35.3)-ml-vs.(110.8-±37.8)-ml,-t=-1.525,-P=0.128],-recovery-time-of-gastrointestinal-function-[(20.8-±2.7)-h-vs.(21.3-±2.3)-h,-t=1.797,-P=0.073],-postoperative-morbidity-[6.3%(10/159)-vs.7.3%(12/165),χ2-=0.124,-P=0.725],-and-the-rate-of-conversion-to-laparotomy-(4.2%vs.2.9%,χ2-=0.397,-P=0.529)-between-the-two-groups-.Both-the-groups-were-followed-up-at-1,-2,-and-6-months-after-procedure-,-and-none-of-the-patients-showed-severe-postoperative-complications-.-Conclusions-Total-laparoscopic-hysterectomy-for-complicated-hysteromyoma-is-feasible-.To-prevent-complications-,-it-should-be-converted-to-laparotomy-timely-when-the-myoma-has-a-wide-base-or-is-located-at-lower-position-.

Objective To investigate the craniofacial morphologies of Sagliker syndrome.Methods Sixteen patients were selected in this study that was diagnosed by the Department of Nephrology and the Center of Dentistry of China-Japan Friendship Hospital.They were divided into 2 groups:Sagliker syndrome group(SS) including 8 patients,and secondary hyperparathyroidism group (SHPT) including 8 patients.Cephalometric radiographs were taken from all the 16 patients.Results The hard issue measurement results had shown that in ∠SNA,∠ANB,∠NA/PA,∠MP/SN,∠MP/ FH,∠SArGo,and ∠ArGoGn,there were significantly statistical differences between the two groups.The measurements of SS were greater than that of SHPT.The soft issue measurement results had shown that in ∠LL-Bs-Pos,∠AsUL-FH,UL-EP,and ∠AsUL-BsUL,there were also significant differences between the two groups.The measurements of SS were greater than that of SHPT.In ∠BsLL-FH,there were evidently statistical differences between the two groups:the measurements of SS were greater than that of SHPT.Conclusions The facial hard and soft tissue of the patients with Sagliker syndrome have the special changes in morphologies.

OBJECTIVE:To investigate general regularity and characteristics of adverse drug reactions/events(ADR/ADE)in-duced by Dezocine injection,and to provide reference for safe and rational drug use in clinic. METHODS:Retrieved from Jiangsu Province ADR Database during Jan. 2009-Aug. 2016,ADR/ADE induced by Dezocine injection was analyzed statistically in re-spects of patient's gender and age,usage and dosage,organs/systems involved in ADR,clinical manifestations and outcome. RE-SULTS:Among 1290 ADR/ADE induced by Dezocine injection,there were 40 cases of severe ADR/ADE(3.10％);medical insti-tutions were main reporting units (1276 cases,98.81％). In ADR/ADE cases,female was more male,with ratio of male to fe-male was 1:1.45. ADR/ADE mostly occurred in 45-65 age group(43.49％). Intravenous medication was main route of administra-tion (1137 cases,88.14％). Initial dose of 577 cases was excessive dose (50.75％). ADR/ADE mainly involved gastrointestinal system (54.99％),central and peripheral nervous system (24.72％) and systemic lesions (5.20％). Severe ADR/ADE manifested as anaphylactic shock,palpitation and dyspnea,etc. But anaphylactic shock was not mentioned in drug package inserts. 1283 ADR/ADE(99.46％)were cured or improved . CONCLUSIONS:It is necessary to strengthen drug safety reevaluation of Dezocine injec-tion after being on the market,improve drug package inserts and enhance patients education as so as to reduce and avoid the occur-rence of ADR/ADE.

Objective To analyze the differences with the expression of glucocorticoids receptor isoforms ( GRα, GRβ, GRγand GR-P ) and cytokines [ IL-6, macrophage migration inhibitory factor (MIF), IFN-γand IL-10] between patients with systemic lupus erythematosus (SLE) and rheumatoid ar-thritis ( RA) , and to further understand their correlations with disease activities.Methods Fifty-five pa-tients with SLE, forty-nine patients with RA and thirty-eight healthy subjects were recruited in this study. All patients were steroid-naive.The expression of GRα, GRβ, GRγ, and GR-P in peripheral blood mononu-clear cells at transcript levels were determined by real-time PCR.Enzyme-linked immunosorbent assay was used to detect the expression of IL-6, MIF, IFN-γand IL-10 in serum samples.Results The percentages of GRαin all subjects were the highest among four isoforms of GR, followed by GR-P, GRγand GRβ.Com-pared with healthy subjects, patients with SLE or RA showed significantly decreased expression of GRα( P<0.05), but increased expression of GR-P (P<0.05).The percentages of GR-P in patients with RA were higher than those in patients with SLE (P<0.05).The expression of GRαwas negatively correlated with SLE disease activity index (SLEDAI) and disease activity score 28 (DAS28).SLE or RA patients with high disease activity showed lower expression of GRαthan those with low disease activity.The levels of IL-6, IFN-γand MIF in patients with SLE or RA were significantly higher than those in healthy subjects ( P<0.05).A negative correlation was observed between the expression of IL-6 and GRαin patients with SLE (P<0.05).The expression of IFN-γwas negatively correlated with GRαin patients with RA (P<0.05). Conclusion There were significant differences with the expression of GR isoforms among patients with SLE, patients with RA and healthy subjects, indicating the change of internal environment in patients might be in-volved in GR alternative splicing.GRαwas the predominant isoform and was negatively correlated with dis-ease activities.Oversecretion of cytokines resulted in a decreased expression of GRα.This study would be useful for the diagnosis of the disease status and for monitoring clinical treatment.

Objective To study the relationship between the expression of perforin, granzyme B and the cytotoxicity of NK cells, after NK cells were purified and expanded with different cytokines. Meth-otis It was detected that the mRNA expressions of perforin, ganzyme B from 8 donors, and the cytotoxcity of NK cells to target I(562 cells by competitive qualitative RT-PCR. Results The mRNA expressions of perforin, granzyme B by purified and expanded NK cells with different cytokines were markedly enhanced, and IL-2 +IL-15 group, IL-2 + IL-12 + IL-15 group were significantly higher than others. It is consistent that the relationship between the mRNA expressions of NK cells and the cytotoxicity of NK cells to I(562 cells. Conclusion The mRNA expressions of perforin, granzyme B of NK cells with different cytokines were obviously.

[Abstract ] Objective The spleen plays an important role in brain ischemia-induced cerebral injury.This study aimed to ex-plore the correlation of the spleen mass index with the neurological function scores and infarction volume following permanent occlusion of the middle cerebral artery ( pMCAO) in rats. Methods Thirty male SD rats were equally randomized into a sham operation, a 3-day brain ischemia, and a 7-day brain ischemia group.The pMCAO model was established by ligation in the right brain of the rats. Neurological function scores were obtained with the Longa 5-Point Scale at 0, 3, and 7 days after modeling, and at 3 and 7 days, the spleen mass index was calculated, the infarction volume measured by TTC, and brain histopathological changes evaluated by HE stai-ning. Results Compared with the 7-day ischemia group, the 3-day ischemia rats showed significantly reduced spleen mass index ([1.62 ±0.58] vs [0.87 ±0.59] mg/g) and increased neurological function score (1.00 [1.00, 1.25] vs 2.00 [1.75, 2.25]) and infarct volume ([18.67 ±7.92] vs [36.20 ±14.92]%) (all P<0.05).An extremely significant decrease was found in the spleen mass index of the 3-day ischemia rats in comparison with that of the animals in the sham operation group ([1.90 ±0.22] mg/g) (P<0.01).HE staining revealed more obvious pathological injury of the cerebral cortex in the 3-day than in the 7-day group.The spleen mass index was negatively correlated with the neurological dys-function score (r=-0.851, P=0.019) and infarction volume (r=-0.717, P =0.013). Conclusion In pMCAO rats, measure-ment of the spleen mass index contributes significantly to the preven-tion and improvement of ischemia-induced cerebral injury.

Objective To evaluate the efficacy and safety of the conscious analgesia/sedation scheme with remifentanil and propofol on the elderly patients under gastroenterological endoscopic procedure. Methods Retrospective analysis was performed on 3 500 cases with remifentanil and small?dose propofol under gastroenterological endoscopic procedures, and the procedure achievement, effects of the narcotic, adverse reactions and satisfactory results were summarized. Results All procedures were completed successfully. The ratio of the patients whose intended sedation depth arrived at referential induction was up to 91?3%(3 196/3 500).Hemodynamic change was slight and almost all patients could open eyes in no time, with short hospital stay. No serious adverse effects were observed except several patients complaining about mild bucking, abdominal distension and stomachache. The degree of patients′ satisfaction was high. Conclusion The conscious analgesia/sedation scheme with remifentanil and propofol can be effectively and safely used on gastroenterological endoscopic procedure among the elderly, but precise monitor and timely treatment of complications are still needed.