Base on the goal of the cultivation in the higher vocational education,this article provides some specific suggestions and recommendations with the thorough thoughts pertaining to the text materials, teaching format,test methods and construction of pharmaceutical microbiology technique training base in the class of pharmaceutical microbiology for the higher vocation education,They are:compiling text books with technology as a main stream,theoretical teaching serving practical teaching,training technical skills with modules,executing two to three alternating experiments arranged both scientifically and reasonably, utilizing an examination approach with a combination of oral tests,on-site operational evaluation,and writ- ten tests,constructing a realistic training base by modeling pharmaceurical production enterprise.

Objective To explore the changes of CX3CR1 expressions in monocytes from the patients with different types of coronary heart diseases (CHD). Methods The expressions of CX3CR1 in monocytes were measured by direct immunofluorescence associated with flow cytometry (FCM) in 54 patients with stable angina pectoris (SAP) confirmed by coronary arteriography, 61 patients with acute coronary syndrome (ACS), 20 normal individuals and 24 patients with chest pain syndrome (CPS). Gensini score system was utilized to quantitatively assess the coronary lesion in CHD patients. Finally, the correlation of CX3CR1 levels and Gensini scores was analyzed. Results CX3CR1 expressions in monocytes from the CHD patients were markedly higher than those in normal individuals (P

Objective To observe the effect of acupuncture plus rehabilitation on the development quotient in patients with mental retardation (MR) and its therapeutic efficacy by using the Neural and Mental Development Check List for Children Aged 0-6. Method Sixty MR patients were divided into a treatment group and a control group by the random number table, 30 cases in each group. The treatment group was intervened by acupuncture plus rehabilitation training, while the control group was by rehabilitation alone. The improvement of development quotient and therapeutic efficacy were evaluated after 3 treatment courses. Result The improvement of development quotient in the treatment group was more significant than that in the control group; the total effective rate was 83.3% in the treatment group versus 73.3% in the control group, and the difference was statistically significant (P<0.05). Conclusion Acupuncture plus rehabilitation training can produce a more significant efficacy than rehabilitation alone in treating MR.

Objective To observe the therapeutic efficacy of acupuncture plus speech training for speech disorders in children with cerebral palsy based on the sign-significant relations (S-S) method for checking language development delay and dysarthria score. Method Ninety patients with spastic cerebral palsy presenting with speech disorders were randomized into a treatment group and a control group by random number table, 45 cases in each group. The treatment group was intervened by acupuncture plus speech training, while the control group was by speech training alone. The improvement of speech function and the relationship between age and therapeutic efficacy were evaluated after 3 treatment courses. Result The total effective rate was 82.2% in the treatment group versus 60.0%in the control group, and the difference was statistically significant (P<0.05);in the treatment group, there were significant differences in comparing the therapeutic efficacy between different age groups (P<0.05). Conclusion Compared to speech training alone, acupuncture plus speech training can produce a better efficacy in treating speech disorders in cerebral palsy;the younger the patient, the better the prognosis.

Arsenic is one of the important metalloid elements, which is widely distributed in nature. Arsenic can cause many health hazards. However, the toxic mechanisms of arsenic have not yet been fully understood. In recent years, many studies have shown that epigenetic regulation and control mechanism is associated with arsenic toxicity. Histone modification is an important epigenetic regulation and control mechanism, changes in its patterns may be one of the mechanisms by which arsenic alters the gene expression. We reviewed the effects of arsenic on regulation of histone methylation, acetylation, phosphorylation and ubiquitination.

Objective To summarize and excavate technical points of acupoint application to intervene infantile recurrent respiratory tract infection based on literature; To provide basis for formulation the standard of the TCM featured therapy. Methods Articles about acupoint application to intervene infantile recurrent respiratory tract infection from CNKI platform (CNKI), Chinese science and technology journals database (VIP), Chinese biomedical literature database (CBM), and Wanfang database were retrived by computers. The medicine, acupoints, the amount of stimulation and the timing for the treatment were analyzed. Results After systematic search, a total of 62 articles were included in the quantitative study, including 6547 patients. The main sticking medicine of 10 highest used rate were:Asari Radix et Rhizoma, Sinapis Semen, Kansui Radix, Corydalis Rhizoma, Cinnamomi Cortex, Astragali Radix, Atractylodix Macrocephalae Rhizoma, Anhelicae Dahuricae Radix, Caryophylli Flos, and Saposhnikoviae Radix, and most of them used ginger juice as anclliary medicine. The acupointwith the highest frequency was Feishu (99.03%), followed by Danzhong, Gaohang, Tiantu, Dingchuan, and Dazhui (28.59%–62.66%). The acupoint application was on the first days of the first, second and third of the three ten-day periods of the hot season, or the first days of the first ninth, the second ninth and the thrid ninth days during the winter time, one dosage for each day. The single sticking time was suggested as 2–4 hours each time (23.44%). Conclusion This study summarizes and evaluates the articles of acupoint application to intervene infantile recurrent respiratory tract standard formulation. infectionin recent years and summarizes the key technical points, which can provide references for clinical and standard formulation.

Objective To investigate the situation of cognitive demands for disease of discharged patients with T tube,in order to adopt a more rational and effective interventions to meet the needs of patients.Methods 60 patients were investigated with the questionnaire survey designed to gauge patients'cognitive demands for disease and health knowledge.Results The patients had higher cognitive demands for T tube retention time,how to make self-observation and influence of T tube retention on normal work and life,among these patients,they laid different particular emphasis to some items between different sexes.Conclusions Nurses can provide patients with comprehensive,prioritized and individualized health guidance according to cognitive demands of patients,so that the health education quality and work efficiency can be improved.

Objective To exPlore the theraPeutic effect of cycloVirobuxine_D ( CVB_D) on heart failure by obserVing the influences of CVB_D on cardiac function,myocardial morPhology and structure in rats with heart failure. Methods The rat model of heart failure was established by ligating left anterior descending coronary artery,and then the rats were randomly diVided into 5 grouPs,Plus the sham grouP. EchocardiograPhy was used to assess cardiac function,including EF,LVSF,SV,HR,CO and so on. Myocardial tissue section was obtained. Change in the myocardial tissue was obserVed by HE staining. Myocardial ultra_structure was obserVed by using transmission electron microscoPe. Results As comPared with the model grouP,EF and LVSF were significantly increased in medium and high dose CVB_D grouPs (P<0. 05 or P<0. 01),SV was increased in low and medium dose CVB_D grouPs (P<0. 05),CO was increased in low,medium and high dose CVB_D grouPs (P<0. 05),LVIDs was decreased and LVPWs was increased in high dose CVB_D grouP (P<0. 05). Under light microscoPe,changes of myocardial structures were obserVed in different CVB_D dose grouPs: myocardial fiber gradually became tidy, striPes became clear, and contour became clear. The changes of myocardial ultra_structure were obserVed under transmission electron microscoPe: the myocardial fiber breakage was reduced and the number of mitochondria was increased,swelling degeneration was alleViated. Conclusion CVB_D significantly imProVed cardiac contraction and blood PumPing function of rats with heart failure, exerted mitigatiVe effect on damage of cardiac muscle fiber and mitochondria. It suggests that CVB_D may be effectiVe in the treatment of heart failure.

Objective To investigate the expression of secretagogin(SCGN) and chromogranin A(CgA) in pancreatic neuroen‐docrine tumors (PNETs) .Methods Totally 54 cases of PNETs hospitalized in our hospital from October 2007 to October 2013 were enrolled in our study .Immunohistochemical detection was used to determine secretagogin and CgA .Results Secretagogin and CgA were highly expressed in all PNETs specimens .Compared with CgA ,secretagogin were highly expressed in the non‐functional PNETs ,tumor volume>30 cm3 ,the enveloped ,vascular invasion and lymph node metastasis (P<0 .05) .There were no statistically significant difference between SCGN and CgA in functional PNETs tumor volume ≤30 cm3 ,no enveloped ,no vascular invasion and lymph node metastasis(P>0 .05) .Conclusion The expression of secretagogin and CgA in tumor combined with PNETs pathologi‐cal characteristics can predict the severity of PNETs .

Objective To explore the clinical and peripheral blood characteristics of childhood purulent meningitis complicated with subdural effusion. Methods A retrospective cohort chart review was performed. A total of 162 consecutive cases of purulent meningitis were identified in Shenzhen Children′s Hospital from January 2009 to De-cember 2013,all of them were divided into 2 groups according to whether complicating with subdural effusion, and the clinical characteristics of 2 groups were analyzed. The clinical characteristics were analyzed. The statistical data were determined by SPSS 17. 0 software. Results Forty-nine cases(30. 25%)of them were complicated with subdural effu-sion;and 37 cases (75. 5%)of them suffered from the disease in the first 10 days after the onset of the meningitis,and the incidence was 48. 84% (42 cases) in infancy. Fever,lethargy,seizures and bulging fontanelle were its common clinical signs,and there were no significant differences in the incidence between subdural effusion group and the control group(non-subdural effusion group),the white blood cell (WBC) count did not increase markedly and C-reactive pro-tein(CRP) was high in the subdural effusion group on the admission day(Z=1. 74,2. 10,all P<0. 05),but no differ-ence in platelet( PLT) . The PLT was high in the whole meningitis cohort,the ratio of cases whose PLT was higher than 300×109/L reached 93. 75%,but no difference between the 2 groups. There were many factors of WBC on admission, including age, cerebrospinal fluid culture results and the application of antibiotics before admission, but CRP was only affected by antibiotics. Conclusions Subdural effusion is common in infancy,and most occurred in the first 10 days af-ter onset of the meningitis. On admission day the CRP,was high, while WBC did not increase too much. If CRP rises for the second time it might predict the occurrence of subdural effusion in bacterial meningitis. The increased PLT may be considered as clinical feature of infectious diseases. The clinical implication of inflammatory pathophysiology in men-ingitis needs further investigation.