Ligustrazine which has extensive pharmacological effects has been clinically used in the treatment of cardiovascular and cerebrovascular diseases,respiratory diseases,urinary diseases,cirrhosis,cancer,etc.Research of protective effects of Ligustrazine on spinal cord injury has recently increased year by year.Numerous clinical trials and animal experiments suggest that Ligustrazine can play a role on ischemic cerebrovascular disease, degenerative brain disease,epilepsy,spinal cord injury and so on,whose main mechanisms include inhibiting of apoptosis and inflammation,enhancing the body’s antioxidant level,improving hemorheology,increasing expression of neurofilament protein and neurotrophic factors,and regulating balance of the ion intracellular and extracellular.Through consulting the relevant literature since 2003,this article reviews the protective roles and mechanisms of Ligustrazine on cerebra and spinal cord injury for the past 10 years.

BACKGROUND: Thrombotic diseases always accompany with blood rheological abnormality. Whether allicin has effects on hemorheological indexes and physiological characteristics of erythrocyte needs to further study.OBJECTIVE: To investigate the effect of allicin on hemorheological indexes and physiological characteristics of erythrocyte in rats with acute blood hyperviscosity.DESIGN: Purely randomly controlled animal study.SETTING: College of Food Science and Nutritional Engineering, China Agricultural University.MATERIALS: A total of 50 Wistar rats of half gender, weighing (200±20) g and aged from 3 to 4 month were provided by Beijing Haidian Tongli Experimental Animal Cultivation Center. Allicin solution was provided by Shanghai Hefeng Pharrnaceutical Co., Ltd. (cencentration: 15 g/L; batch number: 20051001). The main equipments were detailed as follows: NXE-1 cone-plate viscometer (Chengdu Device Factory), DXC-300 nuclear pore membrane erythrocyte deformation radiometer (Device Factory Affiliated to Shanghai Medical University), 650-60 fluorescence polarization (Hitachi Company, Japan) and trace high speed centrifugation (Huaxing Aviation Aircraft Company, Aerospace Faulty).METHODS: The experiment was carried out in the Laboratory of College of Food Science and Nutritional Engineering,China Agricultural University from February to April 2006. All rats were randomly divided into 5 groups, including control group, model group, low-dosage allicin group, moderate-dosage allicin group and high-dosage allicin group, with 10 in each group. Except control group, rats in other 4 groups were perfused with 5 mL tap-water every day fcr 7 successive days. On the seventh day, rats were subcutaneously injected with 0.1% 0.8 mL/kg adnephrin; 2 hours later, rats were dripped in iced water for 5 minutes; 4 hours later, the rats were injected again to establish models of acute blood hyperviscosity. Two days later, all rats in the control group and model group were intraperitoneally injected with saline hematocrit and erythrocyte aggregation index were measured with viscosity determination method; erythrocyte filtration index was measured with nuclear pore membrane flow rate method; erythrocyte membrane fluidity (erythrocyte fluorescence polarization) was measured with fluorescence polarization technique; erythrocyte osmotic fragility test (concentration of NaCl solution from the beginning hemolysis to complete hemolysis) was performed to compare various indexes among different groups.MATN OUTCOME MEASURES: ① Effect of allicin on erythrocyte aggregation (whole blood viscosity,plasma viscosity,hematocrit and erythrocyte aggregation index);② effect of allicin on erythrocyte deformability index (filtration index);③ effect of allicin on erythrocyte osmotic fragility (concentration of NaCl solution from the beginning hemolysis to complete hemolysis);④ effect of allicin on erythrocyte membrane fluidity (erythrocyte membrane fluorescence polarization).RESULTS: Among 50 rats, 8 rats did not have enough blood collection or died; therefore, other 42 rats were involved in the final analysis. ① Effect of allicin on erythrocyte aggregation: As compared with that in the model group, whole blood viscosity was decreased 14.43%, 9.71% and 4.15% in high-dosage, moderate-dosage and low-dosage groups,respectively (P ＜ 0.05-0.01); meanwhile, whole blood viscosity of low shear rate was decreased 15.89%, 14.27% and 6.86%, respectively (P ＜ 0.01), and plasma viscosity was decreased 11.33% (P ＜ 0.05), 9.78% (P ＜ 0.05) and 3.49% (P＞ 0.05), respectively. And the effects mentioned above were shown a dose-effect relationship. Allicin in a high dosage could obviously decrease hematocrit (P ＜ 0.05) and the decreasing degree was 6.47%.② Effect of allicin on erythrocyte deformability index: Filtration index was higher in the model group than that in the control group (0.228±0.025, 0.175±0.043, P ＜ 0.05); however, filtration index was lower in the high-dosage allicin group (0.176±0.034) than that in the model group (P ＜ 0.05). ③ Effect of allicin on erythrocyte osmotic fragility: Concentration of NaCl was higher in the model group than that in the control group at the beginning of hemolysis and at the phase of complete hemolysis. Moreover, allicin in various dosages could improve the increase of erythrocyte osmotic fragility, but there were no significant differences among different dosage groups. ④ Effect of allicin on erythrocyte membrane fluidity: Erythrocyte membrane fluorescence polarization was increased in the model group, and this suggested that membrane fluidity was decreased. Allicin could improve the decrease of erythrocyte membrane fluidity, but there were no significant differences among various dosage groups.CONCLUSION: Allicin can raise erythrocyte deformability, decrease blood viscosity, improve blood fluidity, prevent and cure blood stasis syndrome.

In the past, panretinal photocoagulation (PRP) and vitrectomy (PPV) were the main treatments for proliferative diabetic retinopathy (PDR). In recent years, anti-vascular endothelial growth factor (VEGF) drugs have been used more and more widely in PDR due to their advantages in rapidly subtracting new blood vessels, reducing leakage, and promoting the absorption of blood. The combination of anti-VEGF drugs and PRP in the treatment of PDR, especially high-risk PDR, can increase the rate of neovascularization and prevent some patients with mild to moderate vitreous hemorrhage from PPV. The application of anti-VEGF drugs during the perioperative period of PPV can also reduce bleeding during the operation, shorten the operation time, and reduce surgical complications. Although clinical studies have confirmed that anti-VEGF drugs can be used as an alternative treatment for PRP, most patients require multiple and long-term treatments, which increase the psychological and economic burden of patients. It is expected that the cost of anti-VEGF drugs and the development of long-acting dosage forms can be reduced and bring better efficacy and benefits to PDR patients in the future.

Objective To prepare icariin-loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles (icariin nanoparticles) and analyze their physical and chemical properties,the release of icariin,and the effects on human osteoblast cells in vitro.Methods Icariin nanoparticles were prepared by ultrasonic emulsification and dialysis method and characterized by transmission electron microscopy and particle size analyzer.The release studies in vitro were adopted to evaluate the release-control features.Thiazolyl blue tetrazolium bromide (MTT),alkaline phophatase activity assay,and calcium nodes dyeing were carried out to investigate the effects of icariin nanoparticles on the growth and differentiation of human osteoblast cells MG-63.Results The icariin nanoparticles had spherical shapes and uniform particle size,and the mean size of the nanoparticles was (185.8±5.6) nm.The icariin nanoparticles showed the best property of sustained release and biocompatibility.Meanwhile,the icariin nanoparticles had no effect on the growth of MG-63 ceils,but they promote the differentiation of osteoblast cells by a concentration-and time-dependent manner.Conclusions The icariin nanoparticles prepared by ultrasonic emulsification and dialysis method have a good effect on the differentiation of human osteoblast cells in vitro and are biocompatible.

Objective To study the inconsistent relation of twin discordancy and the umbilical artery blood flow parameter of systolic/diastolic ratio.Methods 358 pregnant women were divided into the two groups of develop-mental nonidentity( n=67 ) and developmental identity ( n=291 ) according to the criterion that the differences of twins weight exceeded 25 percent.The umbilical artery blood flow parameter of systolic/diastolic ratio were observed. Meanwhile,the difference of weight and the umbilical artery blood flow parameter of systolic/diastolic ratio in the group of developmental nonidentity were observed.Results A significant difference of the umbilical artery blood flow parameter of systolic/diastolic ratio were found in the two groups of developmental nonidentity and developmental identity(7.81%vs 32.56%,P<0.05).There were 56 cases that twins weight exceeded from 25 to 35 percent,7 cases that twins weight exceeded from 35 to 45 percent and 4 cases that twins weight exceeded 45 percent in the group of developmental nonidentity which the umbilical artery blood flow parameter of systolic/diastolic ratio were (23.66 ± 5.78)%,(27.16 ±7.25)% and (33.16 ±4.23)% correspondingly with significant difference(F=23.21,P<0.05 ) .Conclusion The umbilical artery blood flow parameter of systolic/diastolic ratio has significance in diagnosis of twin discordancy.

Objective To establish and stabilize a new HLA-B locus typing strategy, reference strand mediated conformation analysis(RSCA)system and to conform its advantages.Methods Of 27 hematopoitic stem cell transplant patients and 63 potential donors DNA samples were extracted from peripheral blood cells and HLA-B loci were both typed by RSCA and PCR-SSP methods. The (ambi)-(guous) results were identified by using DNA sequencing. Results Among 90 samples, 87/90 ((96.7) %) cases could be designated definitely, but one of them was disagreement with the SSP result which was confirmed by sequencing, and 67/90 ((74.4) %) cases could be typed to allelic level. 1/90 ((1.1) %) case could not be identified by RSCA for its bad PCR results. Only one allele of each 2 samples could be designated and the other could not be identified by RSCA, which were further confirmed by sequencing method, and the results confessed which were known as alleles, but there were no corresponding data in RSCA database. Twenty samples were randomly selected to identify the replication rate of RSCA, and the results demonstrated that the replication rate was 100 %. Among PCR-SSP typing results, about 10 % samples might be typed for 1-3 times to confirm their types. Conclusion RSCA had some advantages such as high resolution, high sensitivity, high accuracy, high replication, finding new alleles, large scale and low cost, and was especially suitable for unrelated donor-recipient (screening).

By participating in the national medical colleges and universities students' clinical skill competition and summarizing the experiences after the competition,we reflected on problems and weakness in the past clinical teaching including weak sterile concept and lack of clinical thinking,humanities,communication skills,teamwork awareness,etc.We should take methods to future improve medical students' clinical training capabilities including strengthening the concept of sterile and clinical skills,promoting training of comprehensive clinical thinking ability and problem-solving ability,emphasizing on humane care and communication between doctors and patients; cultivating teamwork awareness thus to comprehensively enhance the overall quality of medical students.

Objective:To evaluate the effect of semiconductor lasers irradiation after routine root canal preparation on root cannal seal.Methods:60 Single-rooted freshly extracted human teeth were randomly divided into 6 groups(n=10).The crowns were removed at the cementoenamel junction and the roots were endodontically prepared with conventional methods.The roots in groups A and B were irradiated with 1 W semiconductor laser for 20 s,in group C and D were ultrasonically washed for 1 min,in group E and F without any treatment were used as the controls.Then all the roots were filled by vertical condensation of warm gutta-percha.The root cannal seal was evaluated with microleakage measurement.The data was analyzed by ANOVA.The teeth of group B,D and F were sectioned and examined under scanning electron microscope(SEM).Results:The microleakage(mm) of group A,C and E was 1.70±0.82,2.02±0.40 and 4.56±2.72 respectively(A vs E,P<0.01;C vs E,P<0.05;A vs C,P>0.05).SEM observation showed the melting,narrowness or closure of most dentinal tubules in group B,past and/or gutta-percha in the most dentinal tubules of group D.Conclusion:Semiconductor laser irradiation prior to root cannal filling can promote the effects of cannal seal.

OBJECTIVE:To investigate general regularity and characteristics of adverse drug reactions/events(ADR/ADE)in-duced by Dezocine injection,and to provide reference for safe and rational drug use in clinic. METHODS:Retrieved from Jiangsu Province ADR Database during Jan. 2009-Aug. 2016,ADR/ADE induced by Dezocine injection was analyzed statistically in re-spects of patient's gender and age,usage and dosage,organs/systems involved in ADR,clinical manifestations and outcome. RE-SULTS:Among 1290 ADR/ADE induced by Dezocine injection,there were 40 cases of severe ADR/ADE(3.10％);medical insti-tutions were main reporting units (1276 cases,98.81％). In ADR/ADE cases,female was more male,with ratio of male to fe-male was 1:1.45. ADR/ADE mostly occurred in 45-65 age group(43.49％). Intravenous medication was main route of administra-tion (1137 cases,88.14％). Initial dose of 577 cases was excessive dose (50.75％). ADR/ADE mainly involved gastrointestinal system (54.99％),central and peripheral nervous system (24.72％) and systemic lesions (5.20％). Severe ADR/ADE manifested as anaphylactic shock,palpitation and dyspnea,etc. But anaphylactic shock was not mentioned in drug package inserts. 1283 ADR/ADE(99.46％)were cured or improved . CONCLUSIONS:It is necessary to strengthen drug safety reevaluation of Dezocine injec-tion after being on the market,improve drug package inserts and enhance patients education as so as to reduce and avoid the occur-rence of ADR/ADE.

Objective To investigate the regulatory effects of umbilical cord-derived mesenchymal stem cells (UC -MSCs) on Th17 cells and related cytokines in patients with systemic lupus erythematosus (SLE). Methods Human UC-MSCs were isolated and expanded and infused into fourteen SLE patients. Clinical changes were evaluated before and after transplantation by SLE disease activity index (SLEDAI), 24-hour urine protein, serum albumin and complement C3. The percentages of CD3 +CD8-IL17A + T cells in peripheral blood were detected by flow cytometry. Concentrations of plasma IL-6, TGF-β, IL-17A, IL-22were determined by enzyme-linked immunosorbent assay (ELISA). UC-MSCs and peripheral blood mononindependent samples t-test. Results SLEDAI scores decreased significantly at 3 month (7.8±1.2, t=2.19) and 6 month (6.9±0.9, t=4.2) after UC-MSCs transplantation than pre-transplantation level (10.4±0.9, P< 0.05). Twenty-four-hour proteinuria decreased significantly 6 months after MSCs infusion [(1489±260) mg vs (2454±322) mg, t=2.6, P<0.05]. Meanwhile, serum albumin and complement C3 levels had increased significantly since 1 month after transplantation (P<0.05). The percentages of peripheral blood CD3+CD8-IL17A+T cells decreased obviously in 1 week, 1 month and 6 months after UC-MSCs transplantation (all P<0.05). The coculture of UC-MSCs with PBMC from SLE patients resulted in a statistically significant reduction of CD3+CD8-IL17A+T cells percentage in PBMC (P<0.05), but was not in a dose dependent manner. No change of plasma IL+6, TGF-β, IL-17A and IL-22 levels was observed after UC-MSCs transplantation (P>0.05).Conclusion UC-MSCs transplantation down-regulates the percentages of CD3+CD8-IL17A+T cells in SLE patients, which may be one of the mechanisms for its therapeutic effect in refractory SLE.