Base on the goal of the cultivation in the higher vocational education,this article provides some specific suggestions and recommendations with the thorough thoughts pertaining to the text materials, teaching format,test methods and construction of pharmaceutical microbiology technique training base in the class of pharmaceutical microbiology for the higher vocation education,They are:compiling text books with technology as a main stream,theoretical teaching serving practical teaching,training technical skills with modules,executing two to three alternating experiments arranged both scientifically and reasonably, utilizing an examination approach with a combination of oral tests,on-site operational evaluation,and writ- ten tests,constructing a realistic training base by modeling pharmaceurical production enterprise.

Objective To explore the changes of CX3CR1 expressions in monocytes from the patients with different types of coronary heart diseases (CHD). Methods The expressions of CX3CR1 in monocytes were measured by direct immunofluorescence associated with flow cytometry (FCM) in 54 patients with stable angina pectoris (SAP) confirmed by coronary arteriography, 61 patients with acute coronary syndrome (ACS), 20 normal individuals and 24 patients with chest pain syndrome (CPS). Gensini score system was utilized to quantitatively assess the coronary lesion in CHD patients. Finally, the correlation of CX3CR1 levels and Gensini scores was analyzed. Results CX3CR1 expressions in monocytes from the CHD patients were markedly higher than those in normal individuals (P

Objective To observe the effect of acupuncture plus rehabilitation on the development quotient in patients with mental retardation (MR) and its therapeutic efficacy by using the Neural and Mental Development Check List for Children Aged 0-6. Method Sixty MR patients were divided into a treatment group and a control group by the random number table, 30 cases in each group. The treatment group was intervened by acupuncture plus rehabilitation training, while the control group was by rehabilitation alone. The improvement of development quotient and therapeutic efficacy were evaluated after 3 treatment courses. Result The improvement of development quotient in the treatment group was more significant than that in the control group; the total effective rate was 83.3% in the treatment group versus 73.3% in the control group, and the difference was statistically significant (P<0.05). Conclusion Acupuncture plus rehabilitation training can produce a more significant efficacy than rehabilitation alone in treating MR.

Objective To observe the therapeutic efficacy of acupuncture plus speech training for speech disorders in children with cerebral palsy based on the sign-significant relations (S-S) method for checking language development delay and dysarthria score. Method Ninety patients with spastic cerebral palsy presenting with speech disorders were randomized into a treatment group and a control group by random number table, 45 cases in each group. The treatment group was intervened by acupuncture plus speech training, while the control group was by speech training alone. The improvement of speech function and the relationship between age and therapeutic efficacy were evaluated after 3 treatment courses. Result The total effective rate was 82.2% in the treatment group versus 60.0%in the control group, and the difference was statistically significant (P<0.05);in the treatment group, there were significant differences in comparing the therapeutic efficacy between different age groups (P<0.05). Conclusion Compared to speech training alone, acupuncture plus speech training can produce a better efficacy in treating speech disorders in cerebral palsy;the younger the patient, the better the prognosis.

Arsenic is one of the important metalloid elements, which is widely distributed in nature. Arsenic can cause many health hazards. However, the toxic mechanisms of arsenic have not yet been fully understood. In recent years, many studies have shown that epigenetic regulation and control mechanism is associated with arsenic toxicity. Histone modification is an important epigenetic regulation and control mechanism, changes in its patterns may be one of the mechanisms by which arsenic alters the gene expression. We reviewed the effects of arsenic on regulation of histone methylation, acetylation, phosphorylation and ubiquitination.

Objective To summarize and excavate technical points of acupoint application to intervene infantile recurrent respiratory tract infection based on literature; To provide basis for formulation the standard of the TCM featured therapy. Methods Articles about acupoint application to intervene infantile recurrent respiratory tract infection from CNKI platform (CNKI), Chinese science and technology journals database (VIP), Chinese biomedical literature database (CBM), and Wanfang database were retrived by computers. The medicine, acupoints, the amount of stimulation and the timing for the treatment were analyzed. Results After systematic search, a total of 62 articles were included in the quantitative study, including 6547 patients. The main sticking medicine of 10 highest used rate were:Asari Radix et Rhizoma, Sinapis Semen, Kansui Radix, Corydalis Rhizoma, Cinnamomi Cortex, Astragali Radix, Atractylodix Macrocephalae Rhizoma, Anhelicae Dahuricae Radix, Caryophylli Flos, and Saposhnikoviae Radix, and most of them used ginger juice as anclliary medicine. The acupointwith the highest frequency was Feishu (99.03%), followed by Danzhong, Gaohang, Tiantu, Dingchuan, and Dazhui (28.59%–62.66%). The acupoint application was on the first days of the first, second and third of the three ten-day periods of the hot season, or the first days of the first ninth, the second ninth and the thrid ninth days during the winter time, one dosage for each day. The single sticking time was suggested as 2–4 hours each time (23.44%). Conclusion This study summarizes and evaluates the articles of acupoint application to intervene infantile recurrent respiratory tract standard formulation. infectionin recent years and summarizes the key technical points, which can provide references for clinical and standard formulation.

Objective: To study the effect of levosimendan treatment in patients of dilated cardiomyopathy (DCM) with different heart fraction. Methods: A total of 145 DCM patients were enrolled, based on left ventricular ejection fraction (LVEF), the patients were divided into 3 groups: Mild heart failure (HF) group, the patients with LVEF≤45%,n=15, Moderate HF group, LVEF≤40%,n=58 and Severe HF group, LVEF≤30%,n=72. LVEF, left atrial diameter (LAD), left ventricular end-diastolic diameter (LVEDD) and blood levels of BNP were examined and compared at prior and 7 days after levosimendan treatment respectively. Results: Compared with prior treatment, fater7 days of levosimendan medication, LVEF was elevated at certain degree in all 3 groups, while the statistic improvement was only found in Severe HF group (26.06±3.59) % vs (24.79±2.81) %,P<0.05; LAD and LVEDD had no obvious changes with levosimendan medication in all 3 groups,P>0.05. After levosimendan treatment, blood levels of BNP were decreased in all 3 groups as in Mild HF group (604.80±631.87) pg/ml vs (1252.17±1435.39) pg/ml, Moderate HF group (2369.78±2478.59) pg/ml vs (3206.90±2677.15) pg/ml and Severe HF group (4879.63±5302.42) pg/ml vs (6004.46±5041.59) pg/ml, allP<0.05. The differences of BNP level between prior and after levosimendan treatment, the ratio for BNP reduction were similar among 3 groups, allP>0.05. Conclusion: Levosimendan may, in short term, improve the cardiac function in DCM patients with mild, moderate and severe HF with similar degree; while it could not really change the cardiac structure.

OBJECTIVE:To investigate the market demand for residence drug allocation and dispatch in Kunming. METHODS:A questionnaire was carried out about the residence drug allocation and dispatch market in plan.RESULTS & CONCLUSION:The target market of residence drug allocation and dispatch is initially established,and the market holds the promising future in Kunming.

Objective:To investigate the changes of ultrastructure of myocardial cells and the expression levels of tumor necrosis factor α(TNF-α) in myocardium tissue of the diabetic rabbits during early myocardial ischemia and their mechanisms,and to provide the theoretical basis for studying the pathogenesis of diabetic myocardial ischemia injury.Methods:A total of 42 healthy male New Zealand white rabbits were chosen.The method of intravenous injection of alloxan in ear vein was used to establish the diabetic models in 26 rabbits among 42 rabbits;the method of partial ligation of left anterior descending coronary artery by thoracotomy was used to establish the diabetic myocardial ischemia models in 22 model rabbits with diabetes.A total of 16 (modeling succesfully) were divided into diabetic myocardial ischemia 7 d group and 28 d group;other 16 rabbits were used as sham operation groups at the relevant time points(7 and 28 d),and there were 8 rabbits in each group.The ultrastructure of myocardial cells was observed by transmission electron microscope;the apoptosis index (AI) of myocardial cells in ischemic myocardium tissue of the rabbits was detected by TUNEL method;the expression levels of TNF-α in ischemic myocardium tissue of the rabbits were detected by real-time RT-PCR method.Results:In sham operation groups,the nuclei of myocardial cells was bigger,the nuclear chromatin was in uniform distribution,the myofibril ordered arrangement,the sarcomere was neat;the mitochondria between myofibril were rich,and their structures were clear.For the rabbits in diabetic myocardial ischemia group,their early-stage cardiocyte nuclei were irregular in shape,myofibrils was loose in structure,the part of myofibril was broken,and the mitochondrial hyperplasia between the myocardial cells was obvious.Compared with sham operation groups,the AI of myocardial cells and the expression levels of TNF-α in the ischemic myocardial cells of the rabbits in diabetic myocardial ischemia 7 d and 28 d groups were significantly increased(P<0.05),and the indexes mentioned above in diabetic myocardial ischemia 7 d group was higher than those in diabetic myocardial ischemia 28 d group(P<0.05).Conclusion:The destruction of ultrasturctue and obvious apoptosis of the myocardial cells of the rabbits occur in the early stage of diabetic myocardial ischemia.The increasing of expression level of TNF-α may involve in the myocardial ischemic injury.

Objective:To observe the clinical efficacy of the combination of montelukast, budesonide nasal spray and deslorata-dine citrate disodium in the treatment of allergic rhinitis and its influence on inflammatory cytokines. Methods:Totally 94 cases of al-lergic rhinitis patients received treatment in our hospital were selected and divided into the observation group and the control group ran-domly. The control group received budesonide nasal spray and desloratadine citrate disodium, and the observation group received mon-telukast additionally. After 4-week treatment, the changes of clinical symptoms and the contents of IL-6, IL-8 and IL-10 in the two groups were compared. The clinical efficacy of the two groups was also compared. Results:After the treatment, the VAS score of the nasal symptom and category symptoms in the two groups were lower than that before the treatment, and the score of the observation group was lower than that of the control group (P<0. 05). After the treatment, the contents of IL-6 and IL-8 were significantly re-duced while the content of IL-10 was significantly increased in the two groups, and the changes in the observation group were more no-table than those in the control group (P<0. 05). The total effective rate of the observation group was 91. 49%, which was higher than that of the control group (80. 85%, P<0. 05). Conclusion:The combination of montelukast, budesonide nasal spray and deslorata-dine citrate disodium can significantly improve the clinical symptoms, reduce the IL-6 and IL-8 levels and increase the IL-10 content in allergic rhinitis patients.