1.Intervention Effect of Modified Dachengqi Decoction on Intestinal Mucosal Barrier of Severe Acute Pancreatitis Model Rats.
Dan-ping QIN ; Xia WEI ; Guo-dong FANG ; Feng YANG ; Deng-pan LAI
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(12):1482-1489
OBJECTIVETo study the effect of Modified Dachengqi Decoction (MDD) as whole course therapy on mediators of inflammation in severe acute pancreatitis (SAP) model rats, and to compare interventional advantages over intestinal mucosal barrier (IMB) of SAP rats between whole course therapy of MDD and early stage therapy of MDD.
METHODSTotally 190 SD rats were divided into five groups according to random digit table, i.e., the sham-operation group, the model group, the octreotide (OT) group, the early stage MDD treatment group, the whole course MDD treatment group, 38 in each group. SAP models were established with retrograde injection of 5% sodium taurocholate into the pancreaticobiliary duct. Three hours after modeling normal saline (NS) was administered to rats in the sham-operation group and the model group by gastrogavage, once per 12 h.1.35 µg/100 g OT was subcutaneously injected to rats in the OT group, once every 8 h. 0.4 mL/100 g MDD was administered to rats in the early stage MDD treatment group, and 6 h later changed to NS (once per 12 h).0.4 mL/100 g MDD was administered to rats in the whole course MDD treatment group, once every 12 h. The accumulative survival rate and morphological manifestations of pancreas and small intestine were observed under microscope 48 h after modeling. Pathologic scores of the pancreas and small intestine were conducted at 4, 6, 24, and 48 h after modeling. Contents of serum amylase (AMY), alanine transaminase (ALT), and TNF-α were also detected. The expression of high mobility group box protein 1 (HMGB1) in the small intestine tissue was also detected by Western blot. The positive rate of bacterial translocation in mesenteric lymph nodes (MLNs) was observed within 48 h. Correlations between serum TNF-α or HMGB1 in small intestinal tissue and pathological scores of the pancreas or the small intestine were analyzed.
RESULTSThe accumulative survival rate was 100. 0% in the sham-operation group, 79. 2% in the whole course MDD treatment group, 70. 8% in the OT group, 45. 8% in the early stage MDD treatment group, and 37.5% in the model group. At 6 h after modeling, pathological scores decreased more in the whole course MDD treatment group, the early stage MDD treatment group, the OT group than in the model group (P < 0.05). At 24 and 48 h after modeling, pathological scores of the pancreas and the small intestine decreased more in the whole course MDD treatment group and the OT group than in the early stage MDD treatment group (P <0. 05). At 6, 24, and 48 h after modeling, serum contents of AMY and ALT both decreased more in the whole course MDD treatment group, the early stage MDD treatment group, the OT group than in the model group (P < 0.05). At 48 h after modeling serum contents of AMY and ALT both decreased more in the whole course MDD treatment group and the OT group than in the early stage MDD treatment group (P < 0.05). At 6 h after modeling serum TNF-α levels decreased more in the whole course MDD treatment group, the early stage MDD treatment group, the OT group than in the model group (P < 0.05). At 6, 24, and 48 h after modeling the level of HMGB1 in the small intestinal tissue decreased more in the whole course MDD treatment group, the early stage MDD treatment group, the OT group than in the model group (P < 0.05). Of them, HMGB1 levels at 24 and 48 h were lower in the whole course MDD treatment group and the OT group than in the early stage MDD treatment group (P < 0.05). The number of MLNs bacterial translocation at 48 h after modeling was lower in the whole course MDD treatment group and the OT group than in the early stage MDD treatment group and the model group (P < 0.05). Serum TNF-α contents within 6 h were positively correlated with pathological scores of pancreas (r = 0.579, P < 0.01). ROC curve showed that serum TNF-α contents could predict the severity of SAP (ROC = 0.990, 95% Cl: 0.971 to 1.000). HMGB1 in the small intestine was positively correlated with pathological scores of the small intestine (r = 0.620, P < 0.01).
CONCLUSIONSEarly stage use of MDD could effectively reduce the release of TNF-α, while whole course use of MDD could effectively inhibit the expression of HMGB1. The latter could preferably attenuate injuries of the pancreas and the small intestine, lower MLNs bacterial translocation, and elevate the survival rate.
Animals ; Bacterial Translocation ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; HMGB1 Protein ; Intestinal Mucosa ; drug effects ; Octreotide ; Pancreas ; Pancreatitis ; drug therapy ; Plant Extracts ; pharmacology ; therapeutic use ; Rats ; Rats, Sprague-Dawley ; Taurocholic Acid ; Tumor Necrosis Factor-alpha
2.Utility of the third arm in robot-assisted laparoscopic partial nephrectomy for complex renal tumor
Taile JING ; Dan XIA ; Ping WANG ; Jie QIN ; Debo KONG ; Sunyi YE ; Shuo WANG
Chinese Journal of Urology 2017;38(7):507-510
Objective To assess the safety and feasibility of utility of the third arm in robotic partial nephrectomy(RPN) through retroperitoneal approach for complex renal tumor.Methods 36 roboticassisted partial nepbrectomy were performed by one surgeon between November 2015 and January 2017,including hilar tumors in 12 cases,central tumors in 11 cases,endophytic tumors in 7 cases and multiple tumors in 6 cases.A 12 mm camera port is placed 2 fingerbreadth above iliac crest.The lateral and medial robotic trocars are placed in the posterior axillary line and anterior axillary plane respectively parallel to the cameraport trocar.Under direct visualization,the peritoneum is swept medially towards the paramedianplane.The fourth arm trocar is placed in the most medial and inferior aspect of the field approximately 7 cm to 8 cm across and parallel to the medial robotic trocar.Descriptive statistics on patient characteristics,operative parameters,and oncologic outcomes are analyzed.Result The 4-arms retroperitoneal approach was used in all patients without any conversion.Mean console time was 127 ± 21 (98-357) min.Mean ischemia time was 25.5 ± 8.3 (12-38) min,Mean estimated blood lost was 198 ± 201.5 (50-510) ml.No patients required blood transfusion,except one case underwent selective intra-arterial embolization with DSA (digital subtraction angiography) and blood transfusion post-operatively.Pathology revealed renal cell carcinoma in 12 patients,angiomyolipoma in 16 patients,chromophobe renal cell carcinoma in 6 cases,oncocytoma in 2 cases and all had negative surgical margins.Under 3 months follow-up,Mean decrease in eGFR was 4.3 ml/(min · 1.73m2).No patients found recurrence or metastasis.Conclusions The third robotic arm provides the console surgeon maximal independence from the surgical assistant when performing kidney retraction,ligation or clamping of renal hilar vessels in complex renal morbidities.It would be beneficial for patient with merits of retroperitoneal approach and overcomes limited space during RPN.
3.Research and Application of Sunshine Medicine Electronic Monitoring Data Analysis System Based on Business Intelligence Technology
Wenge CHEN ; Kang CHEN ; Ting SHU ; Ping QIN ; Zhongxiao LIN ; Dan TANG
China Pharmacy 2016;27(10):1422-1425
OBJECTIVE:To evaluate the research and application situation of Sunshine Medicine Electronic Monitoring Data Analysis System. METHODS:The Sunshine Medicine Electronic Monitoring data Analysis System based on business intelligence technology was introduced in respects of design process,development,implementation and application example. RESULTS:The whole architecture of the system mainly includes hospital business platform,data integration platform,information processing plat-form and application service platform;the functions of the system include medicine homepage show,single species analysis,antimi-crobial agent analysis,national essential medicine analysis,injection analysis and sunshine medicine analysis. It can monitor the drug utilization in multi-angle and multi-level manners by building data center and creating multidimensional models. Besides,the sys-tem could solve thedrugs unified coding and information controlproblems,data collection and information integrationprob-lems in different hospital,andthe efficient calculation and analysis of a large number of drug use data. It can realize drug analy-sis and monitoring in the hospital,analysis and online monitoring of drugs prescribed by the doctor,finding,warning and evaluat-ing abnormal phenomenon of drug use in the medical institutions,so that it is better for the supervisors to monitor the usage of drugs. CONCLUSIONS:The system with easy operation,flexible monitoring,rich chart shows,comprehensive monitor index has a positive effect on rational medication level.
4.Comparison of robot assistant radical prostatectomy by transperitoneal versus extraperitoneal approach
Dan XIA ; Ping WANG ; Jie QIN ; Taile JING ; Sunyi YE ; Shuo WANG
Chinese Journal of Urology 2016;37(3):165-168
Objective To compare the safety and oncological results of robot-assisted laparoscopic radical prostatectomy performed between transperitoneal and extraperitoneal approach.Methods 79 patients underwent transperitoneal robot-assisted laparoscopic radical prostatectomy (Tp-RLRP).The other 86 consecutive patients went through extraperitoneal robot-assisted laparoscopic radical prostatectomy (Ep-RLRP).Results All procedures were performed successfully without conversion.Total procedure time for Ep-RLRP was shorter than it for Tp-RLRP[(218.1 ± 54.4) min vs.(180.2 ± 47.1) min,P < 0.001].Docking time for Ep-RLRP was longer than Tp-RLRP [(21.5 ± 7.1) min vs.(19.5 ± 5.7) min,P < 0.001] Blood lost of Tp-RLRP was lesser than Ep-RLRP[(122.4 ± 71.0) ml vs.(140.4 ± 90.7) ml,P < 0.05].There were no significant differences between two groups in terms of bowel function recovery,drainage tube reserve,foley catheter duration,etc.There were 19 patients (24.1%)and 4 patients (4.7%)were suffered lymph leakage in two groups separately,P < 0.001.One delayed vesiclurethra hemorrhagic patient and one mesenteric torsion patient were happened in Tp-RLRP group.There were no significant differences between two groups in terms of surgical margin,biochemical recurrence and urine continence.Conclusion Compared to Tp-RLRP,Ep-RLRP can provide similar short-term oncological control,recovery of postoperative urine continence,shorter operation time and lower complication rates.
5. Expression of mir-199a in ulcerative colitis rats induced by 2, 4, 6-trinitrobenzene sulfonic acid/ethanol and study on the effect of tripteryginum wilfordii polyglycoside
Chinese Pharmaceutical Journal 2016;51(22):1934-1940
OBJECTIVE: To explore the expression of miR-199a in ulcerative colitis (UC) rats induced by 2, 4, 6-trinitrobenzene sulfonic (TNBS)/ethanol and the study on the effect of TWP on them. METHODS: Through injecting TNBS/ethyl alcohol acid liquid into the anus of the rats to establish the UC rat model. The colitics commom morphous damage and grade the histopathological score (CMDI) of colon mucosa injury were evaluated. Chip analysis and Real-time PCR were used to verify the expression of miR-199a in each colon mucosa tissue. Based on the expression profile, the downstream target genes mRNA in milwalk database was selected, then the expression of target genes mRNA by Real-time PCR in each group was veritied, at last the relevant signal pathway in the DAVID database was analyzed. Doing these to analyse the target gene mRNA regulated by the miR-199a in the inflammatory activity of UC. RESULTS: Compared with the model group, TWP high dose group was significantly lower on gross morphological damage score and histopathological injury score(P < 0.01). Chip analysis showed that in model group, the expression of miR-199a was significantly higher than the normal group(P < 0.01), and expression of the AZA group was significantly lower than the model group(P < 0.01, P < 0.05). The expression of miR-199a-3p in medium dose group and the expression of miR-199a-5p in high dose group were significantly lower than the model group(P < 0.05). The results of Real-time PCR showed that expression of miR-199a in the model group was significantly increased than that in the normal group(P < 0.01). The expression of miR-199a-3p in TWP medium dose group, high dose group and AZA group were decreased than that in model group(P < 0.05). Meanwhile, the expression of miR-199a-5p in TWP medium dose group was decreased than that in model group(P < 0.05). The gene expression profile showed that FASL was the target gene of miR-199a. In the model group, the expression of FASL was higher than that in the normal group. The expression of FASL in AZA group was significantly decreased than that in the model group(P < 0.01). The results by the Real-time PCR of the target gene FASL showed that in the model group, the expression of FASL was higher than that in the normal group (P < 0.01). The expression of FASL in medium dose group, high dose group and AZA group were significantly decreased than that in the model group (P < 0.01). CONCLUSION: miR-199a is up-regulated in TNBS/Ethanol UC rats, and FASL is the downstream target gene of miR-199a. TWP can reduce the UC's inflammatory effectively and decrease the up-regulated miR-199a in UC. FASL is up-regulated in UC's inflammatory activity. TWP can reduce downstream target gene FASL of miR-199a.
6. Regulation of Tripterygium wilfordii Polycoride Tablet towards miR-146a, miR-146b, and TLR4/MyD88 dependent signaling pathway in ulcerative colitis rat model
Chinese Traditional and Herbal Drugs 2016;47(10):1723-1730
Objective: To study the regulatory effect of Tripterygium wilfordii Polycoride Tadlet (TWPT) towards miR-146a, miR-146b, and TLR4/MyD88 dependent signaling pathway in TNBS/ethanol ulcerative colitis (UC) rat model. Methods: TNBS enema was adopted to build TNBS/ethanol UC rat model. After the modeling procedure, 90 male Wistar rats were divided into six groups, including normal, model, low-, mid-, high-dose TWPT, and azathioprine (AZA) groups, and each for 15 rats. All rats in each group were administered with corresponding medicines for 14 d. After 14 d administration, corresponding colon tissues were taken to undergo general and microscopic evaluation. qPCR was adopted to test the expression of miR-146a and miR-146b. Western blotting analysis and RT-PCR were adopted to test the mRNA and protein expression levels of TLR4/MyD88 dependent signaling pathway related molecular, including TLR4, MyD88, TRAF-6, NF-κB, TNF-α, and IL-1β. Results: DAI, general and microscopic evaluation all showed that TNBS/ethanol UC rat model was successfully established. TWPT could improve UC-related clinical manifestation and promote the colonic mucosa healing procedure and such effect was equal to AZA. qRT-PCR showed that the expression of miR-146a and miR-146b in model group was significantly superior to that in normal group (P < 0.01). Compared with the model group, TWPT and AZA could significantly inhibit the expression of miR-146a and miR-146b (P < 0.01). The mid-dose TWPT showed the strongest inhibitory effect. RT-PCR and Western blotting results showed that the expression of TLR4/MyD88 dependent signaling pathway related molecular in model group was significantly superior to that in normal group either in mRNA or protein levels (P < 0.01). Compared with model group, TWPT could inhibit the expression of each spot in TLR4/MyD88 dependent signaling pathway in a dose-dependent manner. The inhibitory effect of high-dose TWPT towards the above molecular was superior to that in model group either in mRNA or protein levels (P < 0.05). The inhibitory effect of high-dose TWPT towards upstream molecular of TLR4/MyD88 dependent signaling pathway (TLR4/MyD88/TRAF-6/NF-κB) was slightly superior to that in AZA group either in mRNA or protein levels. However, such inhibitory effect towards terminal inflammatory cytokines (TNF-α and IL-1β) was slightly inferior to that in AZA group either in mRNA or protein levels. All the above differences had no statistical significance (P > 0.05). Conclusion: In TNBS/ethanol UC rat model, TWPT could inhibit the expression of miR-146a, miR-146b, and TLR4/MyD88 dependent signaling pathway. The inhibitory effect of TWPT towards pathway and inflammatory cytokines shows a dose-dependent manner.
7. Anti-inflammation of Tripterygium wilfordii Polycoride on Macrophages and Its Regulation to Inflammation via TLR4/NF-κB
Chinese Herbal Medicines 2015;7(2):155-161
Objective: To investigate the inhibitory effect of Tripterygium wilfordii polycoride (TWP) towards the pro-inflammatory factors (TNF-α and IL-1β) on the inflammatory reaction in macrophages induced by LPS and its regulatory effect and influence on the inflammation via TLR4/NF-kB. Methods: The MTT method was adopted to test the effect of drugs, TWP, dexamethasone (DXM) and azathioprine (AZA) on cell growth and to select the appropriate concentration. LPS was used to induce the inflammatory reaction in RAW264.7 cell line of mice. Elisa kit was adopted to test the levels of TNF-α and IL-1β. Western blotting was adopted to test the protein expression of TNF-α and IL-1β. RT-PCR was adopted to test the expression of TLR4 and NF-κB. Results: The inhibiting effect of TWP on the release of TNF-α and IL-1β in a dose dependent manner. The inhibitory effect of three different TWP dose groups is weaker than that in DXM group. However, TWP in high dose is better than AZA on TNF-α and is as strong as AZA on IL-1β. The dose dependent manner also exits in the effect on the expression of TLR4 and NF-κB, the effect is not weaker, but even stronger than that of DXM and AZA. Conclusion: The research shows that down regulation of TLR4 and NF-kB p65 may be one of the mechanisms about the TWP inhibitory effect on TNF-α and IL-1β.
8.Study on effects of Tripterygium wilfordii polycoride in resisting macrophage inflammation and regulating inflammation via TLR4/NF-κB.
Dan-ping QIN ; Yi-jun ZHOU ; Shao-zhu ZHANG ; Jun-min CAO ; Li-yu XU ; Guo-dong FANG ; Jia WANG
China Journal of Chinese Materia Medica 2015;40(16):3256-3261
To investigate the effect of Tripterygium wilfordii polycoride (TWP) on LPS-induced macrophage inflammatory response, particularly the inhibitory effect on inflammatory factors TNF-α and IL-1β and the regulatory effect on inflammation via TLR4/NF-κB. The MTT method was adopted to test the effects of tested drugs, TWP, dexamethasone (DXM) and azathioprine (AZA) on cell growth to define the appropriate concentration. LPS was used to induce the inflammatory reaction in mouse RAW264. 7 cell lines. The Elisa kit was adopted to test the release level of TNF-α and IL-1β. The Western blotting was applied to test the protein expressions of TNF-α and IL-1β. The RT-PCR was adopted to test the expressions of TLR4 and NF-κB. According to the results, TWP could inhibit the release of macrophage inflammatory factors TNF-α and IL-1β in a dose dependent manner. All of TWP groups showed a weaker efficacy than that of the DXM group. But the TWP high dose group revealed a better effect on TNF-α and equal effect on IL-1β compared with the AZA group. TWP show an equal or better effect in down-regulating TLR4 and NF-κB p65 expressions in a dose dependent manner than DXM and AZA. In conclusion, TWP could inhibit TLR4 and NF-κB p65, which may be related to the down-regulation of TLR4 and NF-κB p65 receptor expressions.
Animals
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Anti-Inflammatory Agents
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pharmacology
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Cell Proliferation
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drug effects
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Down-Regulation
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drug effects
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Drugs, Chinese Herbal
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pharmacology
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Humans
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Inflammation
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drug therapy
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genetics
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immunology
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physiopathology
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Interleukin-1beta
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genetics
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immunology
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Macrophages
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drug effects
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immunology
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Mice
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NF-kappa B
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genetics
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immunology
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RAW 264.7 Cells
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Toll-Like Receptor 4
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genetics
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immunology
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Transcription Factor RelA
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genetics
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immunology
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Tripterygium
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chemistry
9.The posterior approach of robot-assisted laparoscopic radical prostatectomy
Dan XIA ; Ping WANG ; Sunyi YE ; Jie QIN ; Debo KONG ; Taile JING ; Chong LAI ; Hongzhou MENG ; Shuo WANG
Chinese Journal of Urology 2017;38(6):421-423
Objective To explore the feasibility and safety of the posterior approach of robotassisted laparoscopic radical prostatectomy and to the approach.Methods From November 2001 to April 2017,32 patients underwent posterior approach of robot-assisted laparoscopic radical prostatectomy.Patients aged 53 to 81 years,with mean of 66.9 years old.Their prostate volumes were 12.0-73.7 ml with an average of 32.9 ml.All patients were diagnosed by prostate biopsy before surgery.The operation time,blood loss and length of hospital stay were recorded.Results All the operations were completed by robotic assisted laparoscopy with no transition to open surgery.The surgery time was 129-210 minutes with an average of 163.6 minutes.The estimated blood loss was 20-200 ml with an average of 59.3 ml.The hospital stay was 8-21 days with an average of 12.8 days.The postoperative hospital stay was 3-13 days with an average of 6.9 days.The time of postoperative catheter removal was 4-14 days with an average of 7.5 days.Postoperative follow-up was 1-6 months.Twenty-four (75%) patients had early recovery of continence,and all (100%) patients regained continence 3-month postoperatively.Conclusion The posterior approach of robotic assisted laparoscopic radical prostatectomy was a safe and effective surgical technique,which was beneficial in early continence recovery.
10.Serotype distribution and drug resistance of Streptococcus pneumoniae in hospitalized patients in Yulin, Guangxi
QIU Dan-ping ; LI Tai-jie ; WANG Bai-lian ; LI Ming-qiang ; XUE Ye ; QIN Mei-xiang ; JIANG Dong
China Tropical Medicine 2022;22(11):1037-
Abstract: Objective To analyze the serotype distribution, drug resistance rate and drug resistance gene carrying of Streptococcus pneumoniae isolates in hospitalized patients, and evaluate the coverage of the vaccine to the serotype of Streptococcus pneumoniae in this area, so as to provide reference for the rational use of antibiotics in clinic. Methods A total of 150 strains of non-repetitive Streptococcus pneumoniae isolated from inpatients from January 2015 to December 2019 were collected for serotyping and antimicrobial sensitivity test. The carrying rates of pbp2b, ermB and tetM were detected by PCR. Results The PCR classification rate of 150 strains of Streptococcus pneumoniae was 93.1%, and the classification rate of capsular swelling test was 100%, and a total of 19 serotypes were divided, mainly 19F and 6B. Children's serotypes were predominantly 19F, 6B, and 15A; adult serotypes were predominantly 19F, 14, and 23F. The coverage rates of the PCV7, PCV10, PCV13 and PPV23 vaccines were 36.8%, 42.1%, 57.9% and 68.4%, respectively. Strains with serotypes of 19F, 6B, 3, and 23F had higher rates of resistance to antimicrobials. The sensitivity of Streptococcus pneumoniae to penicillin was greater than 96.0%. Antimicrobials with significant differences in resistance rates between invasive and non-invasive strains were penicillin, moxifloxacin, and levofloxacin. The percentage of strains carrying both ermB and tetM resistance genes was 96.0%, and the concordance rate between pbp2b, ermB and tetM resistance genes and the resistance phenotype was >98.0%. A total of 10 multi-resistance combinations were detected, with a multi-resistance rate of 62.6%, and the multi-drug resistance pattern of Streptococcus pneumoniae was mainly concentrated in the 19F and 6B serotypes. Conclusion There are significant age differences in the serotypes of Streptococcus pneumoniae in this area. The vaccine currently used has low coverage in this region and therefore offer limited protection to the population. The drug resistance rates of Streptococcus pneumoniae varied significantly among serotypes. Erythromycin and tetracycline are not recommended for clinical treatment of Streptococcus pneumoniae. Penicillin can still be used as the first choice for clinical treatment of Streptococcus pneumoniae infection.