1.Comparison of real-time quantitative PCR and allele-specific oligonucleotide PCR for detections of L858R mutation in epidermal growth factor receptor gene exon 21.
Dan-dan DONG ; Wei-ping LIU ; Yuan TANG ; Yan ZOU ; Mei CAO ; Fang-hua LI
Chinese Journal of Pathology 2011;40(5):338-340
Adenocarcinoma
;
genetics
;
pathology
;
Adult
;
Aged
;
Base Sequence
;
Cell Line, Tumor
;
DNA Mutational Analysis
;
Exons
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Female
;
Genes, erbB-1
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Humans
;
Lung Neoplasms
;
genetics
;
pathology
;
Male
;
Middle Aged
;
Mutation, Missense
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Oligonucleotide Probes
;
Polymerase Chain Reaction
;
methods
;
Real-Time Polymerase Chain Reaction
;
methods
;
Receptor, Epidermal Growth Factor
;
genetics
;
Sensitivity and Specificity
2.Changes of vasoactive factors in lung tissue of newborn piglets with persistent pulmonary hypertension and effect of magnesium sulphate.
Fang LIU ; Shi-min ZHAO ; Dan-hua WANG ; Min WEI ; Su-ping ZHANG ; Dan YAO
Chinese Journal of Pediatrics 2003;41(2):139-140
Angiotensins
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analysis
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Animals
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Animals, Newborn
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Biomarkers
;
analysis
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Endothelin-1
;
analysis
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Hypertension, Pulmonary
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drug therapy
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metabolism
;
physiopathology
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Lung
;
chemistry
;
pathology
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Magnesium Sulfate
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pharmacology
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Nitric Oxide Synthase
;
analysis
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Nitric Oxide Synthase Type II
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Swine
;
Vasomotor System
;
chemistry
3.Screening of Active Fractions from Huanglian Jiedu Decoction against Primary Neuron Injury after Oxygen-Glucose Deprivation.
Zhu-yan HUANG ; Bei-bei PAN ; Chun-yan HUANG ; Yi-lu YE ; Dan-dan LIU ; Yue-ping YU ; Qi ZHANG
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(8):981-987
OBJECTIVETo observe the protective effect of active fractions of Huanglian Jiedu Decoction (HJD) on primary cortical neuron injury after oxygen-glucose deprivation (OGD)/reperfusion (R) injury. Methods Using macroporous resin method, HJDFE30, HJDFE50, HJDFE75, and HJDFE95 with 30%, 50%, 75%, and 95% alcohol were respectively prepared. Then the content of active components in different HJD fractions was determined with reverse phase high-performance liquid chromatography (RP-HPLC). The OGD/R injury model was induced by sodium dithionite on primary cortical neurons in neonate rats. MTT assay was used to observe the effect of four fractions (HJDFE30, HJDFE50, HJDFE75, and HJDFE95) and seven index components of HJD on the neuron viability.
RESULTSRP-HPLC showed active component(s) contained in HJDFE30 was geniposide; baicalin, palmatine, berberine, and wogonside contained in HJDFE50; baicalin, berberine, baicalein, and wogonin contained in HJDFE75. The neuron viability was decreased after OGD for 20 min and reperfusion for 1 h, (P <0. 01), and significantly increased after administered with HJD, HJDFE30, HJDFE50, and HJDFE75 (P <0. 05, P <0. 01). Geniposide, baicalin, baicalein, palmatine, wogonside, and wogonin could increase the cortical neuron viability (P <0. 05, P <0. 01).
CONCLUSIONSHJDFE30, HJDFE50, and HJDFE75, as active fractions of HJD, had protective effect on primary cortical neuron injury after OGD/R. Furthermore, geniposide, baicalin, and baicalein were main active components of HJD.
Animals ; Berberine ; Berberine Alkaloids ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Flavanones ; Flavonoids ; Glucose ; metabolism ; Iridoids ; Models, Animal ; Neurons ; Oxygen ; metabolism ; Rats ; Reperfusion Injury ; drug therapy
4.Identification of different varieties of Rhei Radix et Rhizoma based on chemical analysis.
Jian-hua GE ; Xun-hong LIU ; Hu XU ; Dan-yang XU ; Fa-ping BAI
China Journal of Chinese Materia Medica 2015;40(12):2309-2313
A HPLC method was established to determine the contents of the five anthraquinones and rhaponticin in the different varieties of Rhei Radix et Rhizoma. The difference existed in different varieties. The results showed that rhein and rhaponticin were marker substances which could be used to distinguish palm leaf groups rhubarb and wave leaf groups rhubarb. Authentic rhubarb didn't contain rhaponticin. Falsify rhubarb contains trace amounts of rhein. Rheum tanguticum contains abundant rhein. The ratio value of the content of rhein to chrysophanol could be used to distinguish R. tanguticum from the other two authentic varieties (R. palmatum and R. officinale). The content of rhaponticin varied largely in different varieties of wave leaf groups rhubarb.
Chromatography, High Pressure Liquid
;
methods
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Drugs, Chinese Herbal
;
analysis
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Quality Control
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Rheum
;
chemistry
;
classification
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Rhizome
;
chemistry
;
classification
5.Surveillance & management of out-of-plan re-operations
Wanning LIANG ; Dan WANG ; Mo XING ; Ping BAO ; Bingxun HOU ; Jing LIU ; Fengling WANG ; Xu YANG
Chinese Journal of Hospital Administration 2010;26(2):115-117
A series of surveillance and management actions was taken to minimize out-of-plan re-operations. These actions include: 1) Building and completing the surveillance and management mechanism against unplanned re-operations; 2) Building a real-time surveillance and reporting mechanism; 3) Enhancing quality control and continuous improvement at the operating departments; 4) Enhancing the supervision on regulations implementation by quality control departments of the hospital; 5) Enhancing quality control for medical records; 6) Building a feedback mechanism for surveillance results; 7) Building a penalty and reward mechanism. Efforts for one year and a half on such surveillance and management proved successful, as the incidence of out-of-plan re-operations fell from 1.7% before these actions to 1.0% afterwards.
6.The study of relationship between lung function and IFN-γ, IL-4 levels in peripheral blood in patients with pulmonary tuberculosis
Dan LI ; Debing DU ; Jian HE ; Ping YU ; Peng LIU ; Shizhen LUO ; Dongyun CHEN
Chinese Journal of Primary Medicine and Pharmacy 2011;18(3):301-302
Objective To explore the correlation of lung function and IFN-γ and IL-4 levels in peripheral blood in pulmonary tuberculosis patients. Methods The IFN-γ and IL-4 levels in peripheral blood of 75 pulmonary tuberculosis patients(severe 25 cases,moderate 25 casea,and mild 25 cases) and 30 healthy volunteers were measured by ELISA. Meanwhile FEV1 ,FEV1% and MMEF% of lung function in 75 pulmonary tuberculosis patients were measured.Results The levels of IFN-γ[(0. 204 ±0. 018) μg/L] and IL-4[(0. 523 ±0. 035) μg/L] in peripheral blood were significantly different both between tuberculosis group and control group and among tuberculosis patients ( severe,moderate,and mild) (t =7. 685,6. 374 ,all P <0. 05) ;FEV1 ,FEV1% ,and MMEF% of severe and moderate tuberculosis patients were significantly lower than those of mild tuberculosis patients and normal reference value; In tuberculosis patients, FEV1% and MMEF% were negatively related with IL-4 level in peripheral blood( r = -0. 46, -0. 43, all P < 0. 05 ), also significantly positively related with the IFN-γ level in peripheral blood ( r = 0. 47,0. 45, all P <0. 05). Conclusion Pulmonary tuberculosis morbility may be due to cellular levels of patients. The IFN-γ and IL-4 levels in peripheral blood could affect the lung function of pulmonary tuberculosis patients.
7.Study on the micropermeability of resin-dentin bonding interfaces with ethanol-wet bonding technique.
Dong-ping CHEN ; Dan-dan PEI ; Ya-ke WANG ; Cui HUANG ; A-da-lai-ti ; Si-ying LIU
Chinese Journal of Stomatology 2011;46(12):755-758
OBJECTIVETo evaluate the micropermeability on bonding hydrophobic adhesive to dentin with ethanol-wet bonding under simulated pulp pressure.
METHODSTwenty-four intact human third molars were used in the study. After the enamel of occlusal surfaces was removed, the molars were randomly divided into six groups. Adper Scotchbond Multi-Purpose was used in the control group; in the experimental groups, the dentin surfaces were saturated with ethanol for 20 s (group 1), 1 min (group 2), 2 min (group 3), 3 min (group 4) or with a series of increasing ethanol concentrations before application of hydrophobic adhesive (group 5). All the bonding procedures were done under simulated pulp pressure. After 24 hours, micro-tensile bond strength test were performed on the specimens. Bonding interfaces were observed under laser scanning confocal microscope (LSCM) after the pulp chamber were filled with a water-soluble fluoroprobe rhodamine B for 3 hours.
RESULTSCompared with the control group [(38.14 ± 4.97) MPa], bond strengths in group 1 [(21.02 ± 7.23) MPa] and group 2 [(29.64 ± 3.81) MPa] were statistically lower (P > 0.05), while bond strength in group 3 [(38.40 ± 5.03) MPa], group 4 [(37.26 ± 4.68) MPa] and group 5 [(40.12 ± 5.95) MPa] were similar to the control group (P < 0.05). The images taken by LSCM showed that with extension of ethanol-wet time, the deposition of fluorescent dye in hybrid layer and along the dentinal tubules decreased gradually. Especially in group 5, only spare fluorescent dye deposition could be detected in the hybrid layer.
CONCLUSIONSDentin saturated with ethanol for more than 2 min before bonding hydrophobic adhesive to dentin could provide favorable bond strength and decreased the micropermeability of bonding interfaces under simulated pulp pressure.
Acid Etching, Dental ; Composite Resins ; Dental Bonding ; Dental Cements ; Dental Enamel ; Dental Pulp Cavity ; Dentin ; Dentin-Bonding Agents ; Ethanol ; Humans ; Hydrophobic and Hydrophilic Interactions ; Materials Testing ; Resin Cements ; Tensile Strength ; Water
8.Material for evaluation of notoginseng total saponin preparation induced pseudoanaphylactoid reactions.
Ting-ting YU ; Jie LI ; Jia-wei ZHAO ; Ya-xin ZHANG ; Dan-dan LI ; Ai-hua LIANG ; Guan-ping LIU ; Shan GAO ; Yue GAO
China Journal of Chinese Materia Medica 2015;40(14):2732-2736
The experiment is designed to explore pathological festures and material basis of pseadoanaphylactoid reaction induced by notoginseng total saponin preparation. Mouse pseadoanaphylactoid reaction was used, 50 ICR mice were randomly assigned to control group, positive medicine group, notoginseng total saponin preparation low-dose group, notoginseng total saponin preparation middle-dose group, notoginseng total saponin preparation high-dose group on average. They are treated by intravenous injection of test substance solutions containing 0.4% Evans blue (EB). 30 min later, scores of ear blue staining and quantitation of ear EB exudation were recorded. Another two experiment were repeated in the same way excluding EB, just to. detect the related cytokines in serum using ELISA. We found that the scores of pseudoanaphylactoid reaction in notoginseng total saponin preparation injection middle-dose group and high-dose group was evidently higher than that in control group, suggesting that notoginseng total saponin preparation injection may be can lead to pseadoanaphylactoid reaction. HE staining showed that pseadoanaphylactoid reaction induced by notoginseng total saponin preparation injection is related to inflammation. Histamine, VEGF and TNF-α levels in notoginseng total saponin preparation middle-dose group and high-dose group significantly increased (P < 0.05, P < 0.01) than control group and showed a dose-dependent manner as well as consistent with the degree of ear blue dye. While IL-6 and IL-10 content did not increase significantly in notoginseng total saponin preparation low-dose group and middle-dose group, but they significantly higher than control group (P < 0.05, P < 0.01) when it increased to quadrupe clinical concentrations, eight times of the clinical dose. So pseadoanaphylactoid reaction caused by notoginseng total saponin preparation may be related to histamine, VEGF, TNF-α, and it is possible that IL-6 and IL-10 can play a role when pseadoanaphylactoid reaction achieve a certain high degree.
Anaphylaxis
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chemically induced
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Animals
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Capillary Permeability
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drug effects
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Cytokines
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blood
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Dose-Response Relationship, Drug
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Drug Hypersensitivity
;
etiology
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Mice
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Mice, Inbred ICR
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Panax notoginseng
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adverse effects
;
chemistry
;
Saponins
;
adverse effects
9.Distribution and pharmacokinetics of 20S-ginsenoside Rg3 eyeointment in rabbit eye.
Ji-hua LIU ; Dan LU ; Jin-ping LIU ; Ping-ya LI
Acta Pharmaceutica Sinica 2005;40(3):258-261
AIMTo determine 20S-ginsenoside Rg3 in various tissues of rabbit eye and evaluate pharmacokinetics following its topical application.
METHODS45 rabbits were divided into 9 groups. Aqueous humor, cornea and iris-ciliary body were collected at 0.017, 0.033, 0.083, 0.167, 0.5, 1.0, 1.5, 2.0 and 3.0 h following topically applying 1% 20S-ginsenoside Rg3 eyeointment and the concentrations in eye tissues were measured by HPLC.
RESULTSThe pharmacokinetics of 20S-ginsenoside Rg3 in rabbit eye were described by one-compartment model. The peak times in aqueous humor, cornea and iris-ciliary body were 0.42, 0.16 and 0.19 h, and T(1/2) were 0.1, 0.9 and 0.8 h respectively.
CONCLUSIONThe method of determining 20S-ginsenoside Rg3 in rabbit eyes was established. The pharmacokinetics parameters were determined.
Administration, Topical ; Animals ; Aqueous Humor ; metabolism ; Area Under Curve ; Chromatography, High Pressure Liquid ; Ciliary Body ; metabolism ; Cornea ; metabolism ; Eye ; metabolism ; Ginsenosides ; administration & dosage ; blood ; chemical synthesis ; pharmacokinetics ; Iris ; metabolism ; Ointments ; Rabbits ; Tissue Distribution
10.Effects of Jiji decoction on the cognitive function and oxidative stress in mice with vascular dementia induced by cerebral ischemia/reperfusion.
Feng-xi LIU ; Kai NIU ; Zhi-gang WU ; Gui-ping XUE ; Jian-ming YANG ; Dan-shen ZHANG
Chinese Journal of Applied Physiology 2015;31(2):170-177
OBJECTIVETo determine the effects of Jiji decoction (Traditional Chinese Medicine) on the cognitive function and oxidative stress in mice with vascular dementia (VD) induced by cerebral ischemia/reperfusion.
METHODSThirty-two mice were randomly divided into nonnal group (n = 8), sham group (operation, but no cerebral ischemia/reperfusi6n, n = 8), model group (vascular dementia model induced by cerebral ischemia/reperfusion, n = 8), and Jiji decoction-treated group (vascular dementia model plus treatment with Jiji decoction, n = 8). Fourteen days of treatment after operation, the cognitive behavior was measured in step-through test, spatial probe test and platform test. Afterwards, to assess the levels of oxidative stress, the activity of superoxide dismutase(SOD) and content of malonaldehyde (MDA) in brain of these mice were measured.
RESULTSData from step-through test indicated that the escaping latency of Jiji decoction-treated group was prolonged and the error counts were decreased significantly ( P <0.01) compared with those of model group. Data from spatial probe test indicated that the time of entering darkroom, the time of climbing height and the time of entering bright room in Jiji decoction-treated group were shortened and the counts of climbing height were increased (P < 0.05-0.01) significantly compared with those of model group. Data from platform test showed that the escaping latency of Jiji decoction-treated group was prolonged significantly (P < 0.01) compared with that of model group. Compared with normal and sham group, the activity of SOD was decreased and the content of MDA was increased in model group significantly (P < 0.01). Compared with those of model group, the levels of SOD and MDA in Jiji decoction-treated group were improved significantly (P < 0.01).
CONCLUSIONJiji decoction could improve cognitive function of VD mice. Its mechanism might be related with the inhibition of oxidative stiess in the brain.
Animals ; Brain ; drug effects ; metabolism ; Cerebral Infarction ; physiopathology ; Cognition ; drug effects ; Dementia, Vascular ; drug therapy ; Drugs, Chinese Herbal ; pharmacology ; Malondialdehyde ; metabolism ; Medicine, Chinese Traditional ; Mice ; Oxidative Stress ; drug effects ; Reperfusion Injury ; drug therapy ; Superoxide Dismutase ; metabolism