OBJECTIVETo optimize the techniques for extracting hypotensive active peptides from Agrocybe aegerita.

METHODSThe effects of the liquid/solid ratio, extraction time and temperature, pH value of the initial liquid on the extraction percentage (EP) of the hypotensive active peptides were investigated, and inhibition percentage (IP) of the extracts on angiotensin I-converting enzyme was determined.

RESULTSOptimal extraction of the hypotensive active peptides from Agrocybe aegerita was achieved with the liquid/solid ratio of 40:1, extraction time of 3 h, extraction temperature at 30 degrees Celsius;, and pH=8 of the initial liquid. The EP of the hypotensive active peptides from Agrocybe aegerita could reach 87.7% with IP of the extracts on angiotensin I-converting enzyme of 54.0%.

CONCLUSIONThis method is simple and efficient for extracting hypotensive active peptides from Agrocybe aegerita.

Agrocybe ; chemistry ; Antihypertensive Agents ; isolation & purification ; pharmacology ; Chromatography, High Pressure Liquid ; methods ; Dietary Proteins ; isolation & purification ; pharmacology ; Peptides ; isolation & purification ; pharmacology

To evaluate relationship of maternal hepatic vein Doppler flow parameters and cardiac output (CO) with neonatal birth weight in uncomplicated pregnancies (UP) and pregnancies complicated by fetal growth restriction (FGR) .
 Methods: Hepatic vein impedance index (HVI), venous pulse transit time (VPTT), and CO were measured in women with UP at the 14th-37th weeks and complicated by FGR at the 26th-37th weeks who underwent maternal hepatic hemodynamic and echocardiographic examination during the ultrasonography. After delivery, the birth weight and the birth weight percentile of each neonate in this study were recorded. Correlations among HVI, VPTT, and CO were analyzed.
 Results: In the UP group, HVI, VPTT, and CO changed with the increase of gestation. In the FGR group, HVI was higher, VPTT was shorter, CO and neonatal birth weight were obviously lower than those in the UP at the 26th-37th weeks (P<0.05).
 Conclusion: There is a series of adaptive changes in hepatic venous hemodynamics and CO in UP with the increase of gestation to meet the demand of fetal growth, while the maladaptive changes in hepatic venous hemodynamics and CO in pregnant woman may contribute to FGR.
Birth Weight ; Cardiac Output ; Female ; Fetal Development ; physiology ; Fetal Growth Retardation ; physiopathology ; Hemodynamics ; physiology ; Hepatic Veins ; physiopathology ; Humans ; Infant, Newborn ; Pregnancy ; Ultrasonography, Prenatal