Objective To study the diagnostic value of high-sensitivity C-reactive protein (hs-CRP) and C-reactive protein (CRP) in patients with SARS.Methods The level of hs-CRP and CRP in patients with SARS,general bacterial pneumonia and the control are quantitatively detceted by automatically partielc enhanced immunoturbidimetric assay.Results The quantification level of -hs-CRP and CRP are 0 69?0 62mg/L and 4 4?0 9 mg/L in healthy control respectively,10 79?1 36 mg/L and 98 0?28 9 mg/L in bacterial pneumonia,3 16?3 72 mg/L and 11 0?9 6 mg/L in SARS.Conclusion The serum level of hs-CRP and CRP rese in patients with both SARS and bacterial pneumonia,especially in the latter,which is 2 4 and 7 9 times more than the former.This suggests that hs-CRP and CRP may be goog indicators to differentiate SARS with general bacterial pneumonia.

CK.Efficiency of Myo decreases apparently after heart accident onset is more than 6 hours.Traditional marker,CK,CK-MB,AST,LDH and HBDH still have some efficiency in AMI diagnosis.

Female ; Humans ; Infant ; Male ; Ultrasonography ; Urachus ; abnormalities ; diagnostic imaging

Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Infant ; Influenza A Virus, H1N1 Subtype ; Influenza, Human ; diagnosis ; drug therapy ; Male ; Prognosis

Danshen is one of the traditional Chinese herbal medicines and nas a long history or being used clinically in the treatment of cardiovascular and cerebrovascular conditions such as coronary heart disease and angina pectoris. Tanshinone IIA is a derivative of phenanthrene-quinone isolated from Danshen. It has been reported to be the major bioactive compound of Danshen and has diverse biological effects. Recent studies demonstrated that tanshinone IIA had neuroprotective effects on experimental ischemic stroke through its antiinflammatory, anti-oxidant, anti-apoptosis effects and its inhibitory effect on excitatory amino acid toxicity. In this review, we summarized all the recent progresses on the protective effect of tanshinone IIA on cerebral ischemic stroke. Hopefully, this article will throw some light on further study and application of tanshinone IIA.
Antioxidants ; therapeutic use ; Apoptosis ; Diterpenes, Abietane ; therapeutic use ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Salvia miltiorrhiza ; chemistry ; Stroke ; drug therapy

Objective To investigate the expression changes of microRNA 146a (miRNA146a) in UVBdamaged mouse skin. Methods C57/BL6 mice were divided into dose groups to be irradiated on the back with UVB of 30, 60, 90, 180, 270 mJ/cm2 respectively for 24 hours, and time groups irradiated with UVB of 180 mJ/cm2 for 1, 12, 24 and 48 hours respectively. The mice received no irradiation served as the control. Skin samples were subsequently obtained from the irradiated sites of mice and subjected to real-time fluorescent PCR for the detection of miRNA146a expression as well as immunohistochemical staining (IHC) for the detection of STAT3. Results The expression levels of miRNA146a were 0.01158 ± 0.00098, 0.01083 ± 0.00104,0.00872 ± 0.00031, 0.00851 ± 0.00033, 0.00810 ± 0.00057 and 0.00770 ± 0.00031 in the unirradiated control mice and mice irradiated with UVB of 30, 60, 90, 180, 270 mJ/cm2 for 24 hours, respectively, 0.00730 ±0.00036, 0.00805 ± 0.00035, 0.00810 ± 0.00057 and 0.00837 ± 0.00039 in mice irradiated with UVB of 180mJ/cm2 for 1, 12, 24 and 48 hours, respectively. IHC suggested an intensive expression of phosphorylated STAT3 in mice irradiated with a high dose UVB. Conclusions miRNA146a may play an essential role in the mechanism of UVB-induced photodamge, which is mainly correlated with the negative regulation of inflammatory reaction likely mediated by the JAK/STAT3 signal transduction pathway.

Objective To observe the changes of microRNA141 expression in photodamaged skin of mice irradiated with ultraviolet B (UVB).Methods Eighteen C57/BL6 mice were equally divided into six groups to receive single irradiation on the hair-removed back with UVB of 0,30,60,90,180 and 270 mJ/cm2 respectively.Skin specimens were obtained from the irradiated sites 24 hours after the irradiation and subjected to paraffin embedding followed by hematoxylin-eosin (HE) staining for the observation of histopathological changes.Real-time quantitative PCR was performed to determine the miRNA141 expression in skin specimens,immunohistochemical staining (IHC) to quantify the expression of phosphatase and tensin homolog deleted on chromosome ten (PTEN) protein.The TargetScan database was employed for the prediction of miRNA141 targets,and Gostat analysis for functional clustering.Data were processed using SPSS version 13.0 software,and intergroup comparisons were done by analysis of variance.Results The relative expression level of miRNA141 in skin tissue was 2.1354 ± 0.4289,2.4333 ± 0.2517,2.9328 ± 0.3126,3.4125 ± 0.3606,4.5667 ± 0.4014 and 6.7428 ± 0.5158 in mice irradiated with UVB of 0,30,60,90,180 and 270 mJ/cm2 respectively.There was a positive correlation between the expression level of miRNA141 and radiation dose (r =0.992,P ＜ 0.01).Gostat analysis showed that the targets of miRNA141 were mainly related to transcription regulation and signaling transduction.Moreover,PTEN expression gradually decreased with the increase in radiation dose.Conclusions miRNA141 may be involved in UVB-induced photodamage and inflammatory response,and PTEN may play a regulatory role in this process.

Objective To assess hearing and efferent system functions of autistic children.Methods Tests were performed on 30 Autistic children and 15 normal children to evaluate hearing objectively by using otoacoustic emission (OAE) and auditory brainstem response (ABR).The efferent system functions were analyzed through contralateral suppression in OAE.Results The mean ABR Ⅰ-Ⅴ interpeak latencies (IPLs) in children with Autism were significantly reduced than that in the control group.The amplitudes of OAE at 1 kHz and 2 kHz in autistic children were significantly different in two groups.There were no significant differences in contralateral suppressions between the Autistic children and the control.Conclusion Hearing impairment may be more common in children with Autism than in normal children,while for a few Autistic children,their efferent system functions are affected.

Objective To investigate the changes of phenotypes and function of CD56+T cells during primary HIV-1 infection and their relationship with disease progression.Methods Peripheral blood mononuclear cells (PBMCs) were collected from 53 subjects with primary HIV-1 infection and 31 HIV-1-negative healthy subjects.The percentages of CD56+T cells and the expression of several phenotypic markers on CD56+T cells including CD16, CD161, NKB1, NKG2A, NKp46, NKG2D, NKG2C and CD158a were analyzed by flow cytometry.IFN-γand TNF-αreleased by CD56+T cells with and without K562 stimulation and the levels of cytotoxic molecular CD107a were measured.Results The percentages of CD56+T cells in patients with primary HIV-1 infection were significantly lower than those of healthy subjects (P=0.025). The levels of CD56+T cells were negatively related to the viral loads in plasma samples ( P=0.021, r=-0.316).Compared with healthy subjects, the expression of CD16 (P=0.003), CD161 (P=0.023), NKB1 (P=0.023) and NKp46 (P=0.021) on CD56+T cells were decreased in patients with primary HIV-1 infection.The levels of NKB1 were positively related to the CD4+T cell counts ( P=0.007, r=0.364), but were negatively related to the viral loads in plasma samples (P=0.030, r=-0.299).Sponta-neous secretion of IFN-γand TNF-αby CD56+T cells and the expression of CD107a were dramatically in-hibited in patients with primary HIV-1 infection as compared with healthy subjects ( all P<0.001 ) . Moreover, the killing ability of CD56+T cells against K562 target cells was weakened in patients with prima-ry HIV-1 infection as the levels of IFN-γ-, TNF-α-and CD107a-producting CD56+T cells were significantly decreased (P<0.001 for IFN-γand TNF-α, P=0.016 for CD107a).Conclusion Inhibited expression and altered phenotypes of CD56+T cells were identified during primary HIV-1 infection.Lower levels of cy-tokines and cytotoxic molecular were also detected, indicating the dysfunction of CD56+T cells appeared dur-ing early stage of HIV-1 infection and was associated with disease progression.

Objective To investigate the maturation status of monocytes and their responses to the stimulation of toll like receptor (TLR) ligands during primary HIV-1 infection, and to further understand the correlation between functional status of monocytes and disease progression during primary HIV -1 infection. Methods Peripheral blood mononuclear cells ( PBMCs) were collected from 35 subjects with primary HIV-1 infection and 13 HIV-negative healthy subjects to isolate monocytes .Monocytes were stimulated with LPS and Pam3CSK4, respectively, and cultured for 20 hours.The expression of activaion/inhibitory markers on monocytes were analyzed by flow cytometry before and after stimulation .The secretion of proinflammatory cy-tokines ( IL-1β, TNF-αand IL-6) by stimulated monocytes were detected by ELISA .Results The expres-sion of activation markers CD80, CD86, CD40 and inhibitory marker PD-L1 on monocytes were increased in subjects with primary HIV-1 infection (P<0.001 except for CD86 P=0.01).The level of CD40 was posi-tively correlated with viral load in plasma (P<0.001, R=0.553).Compared with control group, primary HIV-1 infection group showed a less increase in the expression of HLA-DR, CD80, CD86 and PD-L1 on monocytes after stimulation with LPS and Pam3CSK4 (P<0.001), but the secretion of proinflammatory cyto-kines TNF-α(LPS:P=0.004, Pam3CSK4:P=0.012) and IL-6 (LPS:P=0.006) were enhanced in mono-cytes from patients with primary HIV-1 infection.Conclusion Monocytes were activated during primary HIV-1 infection.They secreted higher level of proinflammatory cytokines after stimulation with TLR ligands , indicating monocytes might play a role in microbial translocation and immune activation during HIV -1 infection .