China ; Humans ; Severe Acute Respiratory Syndrome ; prevention & control

China ; Humans ; Respiration, Artificial ; Severe Acute Respiratory Syndrome ; complications ; diagnosis ; therapy

China ; Communicable Disease Control ; Humans ; Public Health

Acquired Immunodeficiency Syndrome ; prevention & control ; therapy ; China ; Health Policy ; Humans

Immune rejection is the leading cause of graft failure,and the main way for preventing corneal graft rejection is the application of immunosuppressive drugs.However,in the recent years,cellular therapy has been a new research hotspot for its targeted effect and fewer side effect.A lot of researches showed that Treg cells which areimportant in inducing and maintaining immunological tolerance could directly induce immune tolerance in cornealtransplantation.In recent years,dendritic cells also are found to have a dual role in the immune system,except as antigen presenting cells to induce immune response.Immature or immunosuppressive cytokine-expressing dendritic cells can induce immune tolerance.Mesenchymal stem cells which have multiple differentiation potential can exert anti-inflammatory effects on immune cells and effectively inhibit organ transplant rejection in vitro and in vivo.As another hotspot besides Tregs,myeloid-derived suppressor cells can inhibit the proliferation of a broad range of immune cells (T and B cells,NK cells,and macrophages),induce T cells apoptosis,and even induce Tregs.This review provides an update of these four kinds of cells on their effects and developments in cellular therapy for experimental corneal graft rejection.

Objective: To establish an HPLC method for determining the content of glucosamine hydrochloride and its prepara-tions. Methods:A Phenomenex Luna NH2 column was used, and the mobile phase was ammonium phosphate buffer (dissolving 3. 5 g dipotassium phosphate and 0. 25 ml ammonium hydroxide into 1 000 ml water, adjusting pH to 7. 5 with phosphoric acid) – acetoni-trile (25 ∶75). The flow rate was 1. 5 ml·min-1 and detection wavelength was 195 nm. The column temperature was 35℃ and the injection volume was 20 μl. Results:The linear range of glucosamine hydrochloride was 1. 6-16. 0 mg·ml-1(r=0. 999 9). The av-erage recovery of glucosamine hydrochloride tablets and capsules was 99. 3% and 99. 5%, respectively, and RSDs were 0. 3% ( n=9). Conclusion:The method is simple, accurate and reliable, and suitable for the quality control of glucosamine hydrochloride and its preparations.

Objective To investigate IL-1?mRNA expression and protein secretion after ultraviolet irradiation in human keratinocyte(KC)originated SCC12F cells.Methods Expression of IL-1?mRNA was detected by Northern blot and the secretion level of IL-1?protein was analyzed by ELISA.Results There was a spontaneous expression of IL-1?mRNA in SCC12F cells in culture system of normal KC,which was increased along with the culture time,and reached the highest expression level in120hours.The secretion level of IL-1?protein was increased in a time-and dose-dependent manner in SCC12F cells after ultraviolet B(UVB)irradiation.Conclusions SCC12F cells may express IL-1?mRNA spontaneously under regular KC media.The secretion level of IL-1?protein shows both time-and dose-effect after UVB irradiation.

Choroid neovascularization (CNV) is pathological proliferation of choroid vascular,accompanying with bleeding and leakage,is one of the major factors caused blindness,so CNV inhibitors have become a research hotspot.At present,researches on inhibitors of vascular endothelial growth factors and their receptors,endogenous angiogenesis factors,redox and inflammatory response related factors,etc,have achieved certain progresses.In addition,as drugs with multiple targets for treatment,many Chinese herbs also show inhibition effect on CNV.This article reviews the research advances in inhibitors for CNV.

Objective To investigate Expressions of CD1a and CD83 molecules in cervical lesions. Methods Immunohistochemical method was used to examine the expressions of CD1a and CD83 molecules in tissue samples from 30 patients with squamous cell carcinoma (SCC) of the cervix, 30 patients with cervical condyloma accuminatum (CA) and 30 patients with cervicitis. Results With the increase in the invasiveness of cervical lesions, there was a decline in the density of immature CD1a+ dendritic cells. The average number of immature CD1a+ dendritic cells per high power field (HPF) was 3.45 in cervicitis tissue, 2.89 in CA tissue, 2.41 in SCC tissue. On the contrast, a significant increase was observed in the density of mature CD83+ dendritic cells in CA tissue and SCC tissue compared with the cervicitis tissue (0.057 celIs/HPF and 0.039 celIs/HPF vs 0.019 celIs/HPF, both P < 0.05). The positivity rates of HPV 16/18 and HPV 6/11 were 56.67% and 3.30%, respectively, in cervical carcinoma tissue, 73.30% and 6.67%, respectively, in CA tissue, 3.30% and 0, respectively, in cervicitis tissue. Conclusions Compared with CA tissue, less mature dendritic cells were observed in cervical carcinoma tissue, demonstrating that the antigen presenting cells in carcinoma tissue are insufficient to mount an adequate immune response to prevent lesional invasion.

Objective To investigate the expression and significance of hypoxia-inducible factor 1 alpha (HIF- 1α), vascular endothelial growth factor (VEGF) and angiopoietin-2 (Ang-2) in tissues of condy- loma acuminatum (CA) in pregnant women. Methods Tissue specimens were obtained from the lesions of 30 pregnant women with CA and 30 non-pregnant women with CA, and from the vulva of 15 normal preg- nant women. By immunohistochemical staining, the expressions of HIF -1α, VEGF and Ang-2 were detected in these specimens. Results The expression rates of HIF -1α,VEGF and Ang-2 were 86.67%, 93.33% and 83.33% in pregnant women with CA, respectively, 63.33%, 66.67% and 53.33% in non-pregnant women with CA, respectively, and 0, 6.67% and 0 in normal women, respectively. Enhanced expressions of HIF -1α,VEGF and Ang-2 were observed in pregnant women with CA compared with the latter two groups (P < 0.05). In pregnant women with CA, a significant correlation was noted between the expression of HIF -1α and VEGF (r = 0.412, P < 0.01) and between the expression of Ang-2 and VEGF (r = 0.460, P < 0.01). Further more, the expression of HIF -1α, VEGF and Ang-2 positively correlated with each other in non-preg- nant women with CA. Conclusion In tissues of CA in pregnant women, HIF-1α,Ang-2 and VEGF are over expressed, which may be related to angiogenesis.