OBJECTIVE:To provide reference for rational use of adjuvant drugs in the clinic.METHODS:By retrospective method,the utilization of top 10 adjuvant in the list of consumption sum and DDDs in our hospital during 2013-2015 were analyzed statistically in respects of consumption sum,DDDs,DDC,B/A,etc.RESULTS:During 2013-2015,the consumption sum of adjuvant drugs in our hospital increased from 32 959 000 yuan to 42 436 500 yuan,and its proportion in total consumption sum increased from 31.28％ to 35.22％.The top 10 adjuvant dosage forms in the list of consumption sum were injection,and varieties included activating blood circulation to dissipate blood stasis,nerve nutrition,immunoregulation drugs.The consumption sum fluctuated but was on the increase in general.Top 10 adjuvant drug types in the list of DDDs were mainly drugs for enhancing tissue metabolism,vitamins,nerve nutrition,activating blood circulation to dissipate blood stasis.In terms of DDC,main dosage form was injection;B/A of these drugs was poor and drug price was in high level.CONCLUSIONS:It is suggested to standardize the utilization of adjuvant drugs,so as to promote the effective,economical and rational use of drugs.

Multi-drug resistance (MDR) of breast cancer is a key factor of breast cancer treatment failure.MicroRNA (miRNA) is a class of endogenous expressed,small,single-stranded RNA molecules,and regulate the expression of genes at post-transcriptional level by combining with target message RNA.MiRNA participate in the mechanisms of drug resistance of breast cancer,and are feasible therapeutic targets for the treatment of drug resistance in breast cancer.Finding and studying miRNA related with drug resistance of breast cancer as well as their mechanisms have become a current research hotspot.

Objective To analyze the multiple factors affecting the postoperative mechanical ventilation supporting time of chil-dren with congenital heart diseases ,and to preferably master the offline period and offer intervention techniques about shortening mechanical ventilation supporting time and improving efficacy .Methods A total of 217 with congenital heart diseases in 2012 from January to October were retrospectively studied to seek the factor affecting the duration of ventilation .The factors including the age ,weight ,cardiopulmonary bypass time(CBP) ,aortic cross-clamping time(ACC) ,preoperative albumin ,preoperative pulmonary infection ,preoperative pulmonary artery pressure ,postoperative complications were analyzed by the stepwise Logistic regression . Results The ventilation supporting time was 0 .5 -312 .0 h ,average(28 .42 ± 2 .99)h .Multivariate stepwise Logistic analysis showed that the postoperative complications ,preoperative pulmonary infection and CPB were mechanical ventilation use time of the main influence factors .Conclusion Postoperative complications ,preoperative pulmonary infection and CPB are the main influencing factors of mechanical ventilation supporting time .

Multi-drug resistance of breast cancer remains a major obstacle for effective treatment,which involves many complicated mechanisms,including drug transport in the body,metabolism and drug targets.Recent researches find that the tradition Chinese medicine not only has good effects in improving the body resistance and general situation of patients and enhancing the effects of chemoradiotherapy,but also plays a vital roles in the muti-drug resistance reversal of breast cancer.

It has been preliminarily demonstrated that CIAPIN1 plays an essential role in normal tissue and is closely related to tumor formatin and development, prognosis and multiple drug resistance. CIAPIN1-based target therapy may provide a novel thinkingpathway in cancer treatment.

Objective: To improve the standardization method for ethanol potassium hydroxide volumetric solution. Methods: A new potentiometric titration method was established and compared with the indicator method described in Chinese Pharmacopeia (2010 edition) . Results:There was no significant difference between the results of the potentiometric method and the indicator method( P>0. 05). Furthermore, the potentiometric titration had significant jump range, good precision and high reliability. Conclusion:The po-tentiometric method can be used in the standardization of ethanol potassium hydroxide volumetric solution.

Scutellarin (SC),a botanical extract,possesses a variety of suppressing free radical generation,anti-inflammatory,cardioprotection and anti-virus activity.Recently,many studies show that SC has inhibitory effects on the growth of many tumors and is a kind of potential anti-tumor drug.Its anti-tumor mechanisms mainly include inducing apoptosis,arresting cell cycle,enhancing the adhesion between cells,inhibiting tumor invasion,reversing multidrug resistance,anti-angiogenesis and so on.

Objective To investigate the effect of COX-2 in the DSS-induced UC mouse model and the effects of probiotics in the pathogenesis of UC. Methods DSS-induced UC mouse model was used in the study. A total of 60 mice were divided into 6 groups (Blank control group, DSS model group, Saline group, SASP group, Lactovacillus group and Bifidobactefia group). After 8 days, all mice were sacrificed. Histological injury score and pathological change and COX-2 in mucous of colon membrane were evaluated. Results The stages and histological changes were obviously improved and the expression of COX-2 in the colonic mueosa of mouse with UC was decreased when using probiotics and SASP. Conclusions Lactobacillns and Bifidobacterla can decrease the expression of COX-2 in the colonic mucosa of mouse with UC. The effect has no difference between SASP and probiotic group.

Objective To investigate the relationship between onset of malnutrition and growth hormone-insulin-like growth factor(GH-IGF) axis in children with congenital heart disease(CHD).Methods Fifty cases with CHD were divided into two groups,13 cases of cyanotic group and 37 cases of acyanotic group.And the acyanotic group was then subdivided into two groups,25 cases of heart failure group and 12 cases of without heart failure group,and 20 normal children were selected as control group.The nutritional status of these children were evaluated by detecting the serum total protein,serum protein,body height and weight.IGF-Ⅰ and insulin-like growth factor binding protein-3(IGFBP-3) and growth hormone binding protein(GHBP) were detected by immunoradiometric assay.Results The acute malnutrition ratio and chronic malnutrition ratio in the children with CHD were 62% and 28%,respectively.The acute malnutrition ratio was similar between cyanotic group and acyanotic group(P=0.105);the chronic malnutrition ratio significantly increased in cyanotic group than that in cyanotic group(P=0.006).The levels of serum IGF-Ⅰ and IGFBP-3 decreased in the patients with CHD,which significantly decreased in cyanotic group and heart failure group.The level of serum GHBP decreased in cyanotic group and heart failure group.Conclusions The levels of serum IGF-Ⅰ and IGFBP-3 in patients with CHD decrease,which are related to growth hormone receptor down regulated,that is one of the molecular mechanisms of growth hormone resistant or insensitivity,growth hormone counteract or insensitivity are one of the reasons of acute and chronic malnutrition in children with CHD.

Objective To explore the myocardial damage and the changes of MB isoenzyme of creatine kinase(CK-MB) and cardial troponin I(cTnI) of rat with endotoxemia at early stage.Methods Sixty SD rats were randomly divided into lipopolysaccharide(LPS) group(LPS group,n=48) and control group(n=12).Rats in LPS group were injected intraperitoneally with LPS(5 mg/kg) and then divided into 6 subgroups according sacrificed time after injection(0.5,1.0,2.0,3.0,12.0,24.0 h).CK-MB/ cTnI in blood was determined with chemiluminescent technique and myocardial pathological damage was observed under the light and transmission electron microscope.Control group was subjected to normal sodium and divided randomly into the same subgroups too.Results The rat diminished their action after beening injected endotoxin 15 minutes,and their reaction were slow.One hour later,they were short of action and reaction.Three hours later,they almost did not move at all,and the glossiness of their pelage were faint.Twenty-four hours later,their foodintake was diminished,their shout were weakened,and their response to stimulus were very thin,the glossiness of pelage were insufficient.Compared with control group,CK-MB increased significantly at 1.0,2.0,3.0,12.0,24.0 h(Pa