Objective: To improve the standardization method for ethanol potassium hydroxide volumetric solution. Methods: A new potentiometric titration method was established and compared with the indicator method described in Chinese Pharmacopeia (2010 edition) . Results:There was no significant difference between the results of the potentiometric method and the indicator method( P>0. 05). Furthermore, the potentiometric titration had significant jump range, good precision and high reliability. Conclusion:The po-tentiometric method can be used in the standardization of ethanol potassium hydroxide volumetric solution.

Objective To investigate the personality characteristics of the drivers in the Armed Police Force ( CAPF), as well as construct a personality traits model for drivers and evaluation criteria. Methods Adopting Sixteen Personality Factor Questionnaire(16PF) to collect data from 1187 CAPF drivers in Beijing,Sichuan,Inner Mongolia and Yunnan. Results The paper concludes that career personality traits of CAPF drivers are made up of 4 factors: stability and self-control, self-motivation and cooperation, fantasy and openness, intelligence and candor, which explain 50.13% of the total variance. Conclusion The four factors basically construct the personality trait model of CAPF drivers. They could also be of great significance in recruiting, training and evaluating drivers.

OBJECTIVE:To establish a method for the content determination of total flavonoids in onion extracts.METH_ ODS:UV spectrophotometry was adopted in the determination with the detection wavelength set at270nm.RESULTS:Good linear relationship with the absorbability was achieved when the detection concentration of eldrin was within a range of0.002～0.01mg/ml(r=0.9978),the average recovery was99.15%(RSD=1.52%).CONCLUSION:This method was simple,rapid and accurate.

Objective:To establish the fingerprints of Rhizoma Atractylodis macrocephalae from different origins with high performance liquid chromatography(HPLC)technology,which was furthermore applied in the quality comparison of plant resource.Methods:In this method,C1 8column(4.6mm?250 mm)was used with the mobile phase containing acetonitrile-water for gradient elution,acetonitrile(A):0-15min,75%;15-20min,75%-95%;20-35min,95%;35-40min,95%-75%;40-50min,75%,flow rate 1 mL/min and wave length 220nm.Results:The HPLC fingerprints of Rhizoma Atractylodis macrocephalae from different origins were established and the correlated coefficients of each were calculated.9 common peaks were determined in HPLC chromatogram,three of them were identified as atractylenolideⅢ,Ⅰand atractylon respectively.Conclusion:The method was simple,reproducible and can be used as plant resource selection and quality control of Rhizoma Atractylodis macrocephalae.

Objective To set up the quality standard of Shenbai concentrated pills. Methods Radix Astragali, Radix Paeoniae Rubra and Rhizoma Imperatea in Shenbai concentrated pills were identified with the method of TLC. The concentration of salvianolic acid B was determined with the method of HPLC. Results For TLC, the chromatogram spots of Radix Astragali, Radix Paeoniae Rubra and Rhizoma Imperatea were well separated and without interference in their negative controls. Quantitative analysis of HPLC showed that the average recovery of salvianolic acid B was 99.74% (RSD=2.08%) with the RSD of precision, reproducibility and stability was 1.74%, 1.19% and 1.70%, respectively. Conclusions The methods set up by this study are easy, accurate and stable, and can be used as the quality control standard of Shenbai concentrated pills.

Objective: To explore the early diagnostic value of dynamic changes for high-sensitive troponin I (hs-cTnI) in patients with acute coronary syndrome (ACS).
Methods: A total of 186 ACS patients treated in our hospital from 2014-02 to 2015-04 were studied. The patients were divided into 2 groups:AMI group, n=169 and UA group, n=17, in addition, there was a Control group, n=13 healthy subjects. Blood levels of hs-cTnI were measured at admission and 2h, 4h after admission. Dynamic changes of hs-cTnI was calculated by slop coefifcient of hs-cTnI (Δhs-cTnI) at 3 time points and relative changes of hs-cTnI values. ROC curve was made by SPSS 16.0 statistical software and the best diagnostic cutoff point was determined by Youden index.
Results: In clinical practice, hs-cTnI=1 ng/ml was critical condition, hs-cTnI=0.15 ng/ml was recommended for AMI diagnosis. When hs-cTnI<1 ng/ml at admission, the AUC of AMI vs UA and AMI vs Control for 0 h hs-cTnI were 0.6746 and 0.8844;for relative changes of hs-cTnI were 0.9806 and 0.9631;forΔhs-cTnI were 0.9521 and 0.9778 respectively. Thus, the best diagnostic cutoff value of AMI for relative changes of hs-cTnI was 0.6705, sensitivity 70.59%, speciifcity 91.49%;forΔhs-cTnI was 0.0075, sensitivity 68.75%, speciifcity 97.78%. When hs-cTnI<0.15 ng/ml at admission, the AUC of AMI vs UA and AMI vs Control for 0 h hs-cTnI were 0.5677 and 0.7000;for relative changes of hs-cTnI were 0.9228 and 0.9975;forΔhs-cTnI were 0.9044 and 0.8906 respectively. Thus, the best diagnostic cutoff value of AMI for relative changes for hs-cTnI was 2.923, sensitivity 81.25%, speciifcity 94.12%;forΔhs-cTnI was 0.01125, sensitivity 76.47%, speciifcity 93.75%.
Conclusion: AMI could not be effectively diagnosed by hs-cTnI alone at admission, using combined relative changes of hs-cTnI andΔhs-cTnI was superior to 0 h hs-cTnI for AMI diagnosis.

To prepare salvianolic acid phospholipid compound. With the compound of salvianolic acids and soybean phospholipid as the index, mono-factor experiment and orthogonal design experiment were conducted to screen its technical parameters. According to the results, the optimal preparation conditions of salvianolic acid phospholipid compound were that THF were taken as the reaction solvent, the concentration time was 3 h, the reactant concentration was 5 g x L(-1), the mass ratio of salvianolic acids and phospholipid was 1: 1.5, and the reaction temperature was 40 degrees C. The oil/water partition coefficient of the prepared salvianolic acid phospholipid compound significant increased in water and buffers with different pH values. The results of phase analysis such as DSC, XRD and FTIR indicated that salvianolic acids existed in phospholipid in an amorphous state.
Alkenes ; chemistry ; metabolism ; Chemical Phenomena ; Chemistry, Pharmaceutical ; methods ; Intestinal Absorption ; Phospholipids ; chemistry ; Polyphenols ; chemistry ; metabolism ; Soybeans ; chemistry ; Temperature

To study the interaction of drugs of different properties, namely puerarin, borneol and catalpol in the process of in- clusion, in order to explore the inclusion regularity of multi-component and multi-property traditional Chinese medicine compound in- clusions. With HP-β-CD as the inclusion material, the freeze-drying method was used to prepare the inclusion. The inclusion between puerarin, borneol and catalpol was tested by measuring the inclusion concentration, DSC and X-ray diffraction. According to the find- ings, when insoluble drugs puerarin and borneol were included simultaneously, and puerarin was overdosed, puerarin included was almost equal to puerarin included, and borneol was not included. When puerarin was under-dosed, and HP-β-CD was overdosed, borne- ol was included, and the simultaneous inclusion was lower than the separate inclusion of borneol. When water-soluble drug catalpol was jointly included with puerarin or borneol, the simultaneous inclusion was almost the same with their separate inclusion, without charac- teristic peak of catalpol in DSC and X-ray diffraction patterns. There is a competition in the simultaneous inclusion between water-solu- ble drugs puerarin and borneol and a stronger competition in puerarin. The water-soluble drug catalpol could be included with HP-β-CD with no impact on the inclusion of puerarin or borneol.
2-Hydroxypropyl-beta-cyclodextrin ; Bornanes ; chemistry ; therapeutic use ; Brain Ischemia ; drug therapy ; Drug Compounding ; methods ; Freeze Drying ; Iridoid Glucosides ; chemistry ; therapeutic use ; Isoflavones ; chemistry ; therapeutic use ; Solubility ; beta-Cyclodextrins ; chemistry

Objective To discuss the influence of social support and educational background on senile patients' mental health after they had been hospitalized, and to table the intervention measure. Methods We made investigation about 80 cases of senile male patients, in terms of their general information (including age, educational background, family status and so on), Social Support Rating Scale (SSRS) score, and viewpoint toward self-mental health. And we analyzed the data in combination with the clinical data of these patients with statistical methods. Results Based on the patients' viewpoint toward self-mental health, comparisons of social support and educational background between the positive group and the negative group was made and there were significantly differences between two groups (P < 0. 05). Conclusions Social support and educational background directly influence senile patients' mental health and their recovery from disease. S, it needs to pay attention to the factors and place forward the active intervention on these as an important point in clinical nursing.

OBJECTIVETo compare the difference of Euphorbia Pekinensis Radix before and after being processed with vinegar in the toxicity on rat small intestinal crypt epithelial cells IEC-6, and make a preliminary study on the mechanism of detoxication of Euphorbia Pekinensis Radix processed with vinegar.

METHODWith rat small intestinal crypt epithelial cells IEC-6 as the study object, the MTT method was adopted to detect the effect of Euphorbia Pekinensis Radix before and after being processed with vinegar on IEC-6 cell activity. The morphology of cells were observed by the inverted microscope. The down-regulated mitochondrial apoptosis pathway of enterocytes caused by the vinegar processing was analyzed by using the high content screening.

RESULTCompared with the negative control group, the proliferation inhibition experiment showed that Euphorbia Pekinensis Radix showed a relatively high intestinal cell toxicity (P < 0.01). The results of HCS analysis showed that Euphorbia Pekinensis Radix could significantly reduce the cell nucleus Hoechst fluorescence intensity and mitochondria membrane (P < 0.05, P < 0.01), and increase Annexin V-FITC and PI fluorescence intensity and membrane permeability (P < 0.01, P < 0.01, P < 0.01). After being processed with vinegar, compared with Euphorbia Pekinensis Radix groups with different doses, Euphorbia Pekinensis Radix processed with vinegar could significantly decrease the cell proliferation inhibition effect on enterocytes, increase the cell nuclear Hoechst fluorescence intensity and mitochondria membrane (P < 0.05, P < 0.05), and decrease Annexin V-FITC and PI fluorescence intensity and membrane permeability (P < 0.01, P < 0.01, P < 0.05), and showed a certain dose-effect relationship.

CONCLUSIONThe vinegar processing can further reduce the toxicity of Euphorbia Pekinensis Radix on enterocytes. Its possible mechanism can decrease the effect of Euphorbia Pekinensis Radix on the permeability of IEC-6 cell membrane, so as to provide a basis for further explanation of the detoxication mechanism of Euphorbia Pekinensis Radix processed with vinegar.

Acetic Acid ; chemistry ; Animals ; Apoptosis ; drug effects ; Cell Line ; Cell Proliferation ; drug effects ; Cell Survival ; drug effects ; Chemistry, Pharmaceutical ; methods ; Drugs, Chinese Herbal ; chemistry ; toxicity ; Epithelial Cells ; cytology ; drug effects ; Euphorbia ; chemistry ; Intestine, Small ; cytology ; Rats