Objective:To explore the clinical characteristics of patients with pituitary thyrotropin-secreting adenoma and evaluate the effect of preoperative short-acting octreotide treatment on hyperthyroidism.Methods:A retrospective analysis was performed in 40 patients with pituitary thyrotropin adenoma diagnosed in Beijing Tiantan Hospital from January 2008 to January 2018. The general data, laboratory examinations and imaging findings were reviewed and analyzed. The clinical effect of preoperative octreotide on hyperthyroidism was evaluated.Results:The age of onset year of the 40 patients (male: female = 24∶16) was (30.5±5.1) years. Among them, 35 patients (87.5%) were with macroadenoma. The most common symptoms were thyroid hypermetabolism syndrome, followed by headache, dizziness, visual field damage and hypogonadism. The thyroid function of 30 patients (75%) recovered to normal within 3-5 days after the octreotide treatment. The total effective rate of the octreotide was 90.0%. The level of free thyroxine (FT 4) before treatment in patients with more than 10 times of effective cumulative dose was significantly higher than that in patients with less than 10 doses. Conclusions:Thyroid hypermetabolism syndrome and pituitary occupying effect are the most common clinical manifestations of thyrotropin-secreting adenoma. Preoperative octreotide treatment can effectively control hyperthyroidism. The level of FT 4 is a crucial factor affecting the efficacy of octreotide.

Breast Neoplasms ; genetics ; Carcinoma ; genetics ; Female ; Gene Amplification ; Humans ; Immunohistochemistry ; In Situ Hybridization, Fluorescence ; Polymerase Chain Reaction ; methods ; Receptor, ErbB-2 ; genetics

Objective To evaluate the role of autophagy in the development of inflammatory pain in the rats.Methods Twenty-four healthy adult male Sprague-Dawley rats,weighing 180-240 g,were randomly divided into 4 groups (n=6 each) by using a random number table:control group (group C),inflammatory pain group (group IP),dimethyl sulfoxide (DMSO) group (group D),and rapamycin (autophagy inducer) group (group R).Inflammatory pain was produced by injecting 50 μl bee venom into the plantar surface of the left hindpaw.In group C,0.9％ normal saline was injected into the plantar surface of the left hindpaw.In group D,2％ DMSO was injected through a gastric tube into the stomach 1 ml per day for 3 consecutive days,and the model was established at 1 h after injection on 3rd day.In group R,rapamycin l0 mg/kg (in 2％ DMSO) was injected through a gastric tube into stomach 1 ml per day for 3 consecutive days,and the model was established at 1 h after injection on 3rd day.The mechanical paw withdrawal threshold (MWT) and thermal paw withdrawal latency (TWL) were measured at 2 h after the model was established.After measurement of the pain threshold,the dorsal horn of the spinal cord was removed for determination of the expression of microtubule-associated protein 1 light chain 3 Ⅱ (LC3 Ⅱ),Beclin-1 and p62 by Western blot.Results Compared with group C,the MWT was significantly decreased,the TWL was significantly shortened in IP and D groups,and the expression of LC3 Ⅱ,Beclin-1 and p62 in the dorsal horn of the spinal cord was significantly up-regulated in IP,D and R groups (P＜0.05 or 0.01).Compared with group IP,the MWT was significantly increased,the TWL was significantly prolonged,the expression of LC3 Ⅱ and Beclin-1 in the dorsal horn of the spinal cord was significantly up-regulated,and the expression of p62 was significantly down-regulated in group R (P＜0.05),and no significant change was found in the parameters mentioned above in group D (P＞0.05).Conclusion Autophagy disorders are involved in the development of inflammatory pain in the rats.

Objective To evaluate the role of hippocampal mammalian target of rapamycin (mTOR) signaling pathway in inflammatory pain in rats.Methods Sixty adult male Sprague-Dawley rats,weighing 180-240 g,were randomly divided into 4 groups (n=6 each) by using a random number table:control group (group C),inflammatory pain group (group IP),dimethyl sulfoxide (DMSO) group and rapamycin (inhibitor of mTOR) group (group R).Inflammatory pain was produced by injection of honey bee venom 50 μl into the plantar surface of the left hindpaw.While the equal volume of normal saline was given instead in group C.In group D,2％ DMSO was injected through a gastric tube into stomach 1 ml per day lasting for 3 days,and inflammatory pain was produced at 1 h after the last injection on 3rd day.In group R,rapamycin 10 mg/kg (in 2％ DMSO) was injected through a gastric tube into stomach 1 ml per day lasting for 3 days,and inflammatory pain was produced at 1 h after the last injection on 3rd day.At 2 h after the model was established,the mechanical paw withdrawal threshold (MWT) and thermal paw withdrawal latency (TWL) were measured.Rats were sacrificed after measurement of pain threshold,and hippocampal tissues were obtained for detection of the expression of mTOR,phosphorylated mTOR (p-mTOR),ribosomal S6 kinase (S6K) and phosphorylated S6K (p-S6K).Results Compared to group C,the MWT was significantly decreased,TWL was shortened,the expression of p-mTOR and p-S6K was up-regulated,and no significant change was found in the expression of mTOR and S6K in IP and DMSO groups,and no significant change was found in group R in the MWT,TWL and expression of p-mTOR,mTOR,p-S6K and S6K.Compared to group IP,no significant change was found in group DMSO in the MWT,TWL and expression of p-mTOR,mTOR,p-S6K and S6K,and the MWT was significantly increased,TWL was prolonged,the expression of p-mTOR and p-S6K was down-regulated,and no significant change was found in the expression of mTOR and S6K in group R.Conclusion Hippocampal mTOR signaling pathways are involved in the development of inflammatory pain in rats.

OBJECTIVETo estimate the coverage level of case reporting for coal workers' pneumoconiosis (CWP) and the incidence of CWP in China according to the data from CWP reporting database in China as well as the data on annual raw coal output, coal workers, etc. from relevant year books, and to provide scientific data for CWP control measures.

METHODSThe data on the numbers of coal workers and annual raw coal outputs of national top 100 enterprises in coal sector (Top 100 in coal sector) were acquired from China Coal Industry Yearbook; the annual per capita output of raw coal in each enterprise was calculated. The numbers of coal workers in the enterprises other than the Top 100 were estimated based on the annual per capita output of raw coal in the Top 100 and the annual raw coal outputs of the enterprises other than the Top 100. The numbers of coal workers in the enterprises that reported CWP cases were estimated based on the distribution of these enterprises in China, and the coverage rates of case reporting in the Top 100 and other enterprises were calculated accordingly. The total number of CWP cases and the number of CWP cases due to per million tons of raw coal output were estimated according to the coverage rates of case reporting and the total numbers of reported CWP cases in China.

RESULTSThe mean coverage rates of case reporting in the Top 100 and other enterprises were 55.34% and 5.18%, respectively. The estimated mean numbers of new CWP cases in the Top 100 and other enterprises were 3088 and 98205, respectively; the estimated total number of new CWP cases nationwide was 101293. The estimated mean numbers of new CWP cases due to per million tons of raw coal output were 2.05 in the Top 100 and 7341 in other enterprises. The number of reported CWP cases accounted for 6.7% of the estimated number of CWP cases (6796/101293).

CONCLUSIONIt is urgent to enhance occupational disease control in coal sector, and the estimation results for CWP should be spread and applied. The system of occupational disease control should be further improved.

Anthracosis ; epidemiology ; China ; epidemiology ; Coal Mining ; Data Interpretation, Statistical ; Humans ; Incidence ; Male ; Occupational Exposure

Objective To prepare chitosan-polyaspartic acid-5 fluorouracil (CTSPasp-5FU) nanoparticles and to investigate its anti-neoplastic effect and toxicity.Methods CTS-Pasly5FU nanopartieles were synthesized by ion gelatifieation.BALB/C nude mice were injected with gastric carcinoma cell line SGC- 7901 mass subcutaneously near nape to establish human gastric carcinoma model.Then they were randomly al- located into chitosan-polyaspartie acid -5fluorouracil(CTS-Pasp-SFU,containing 5-FU 1.25mg/kg) group, 5-FU (1.25mg/kg) group and normal saline group.Tumor weight was measured and the colony forming unit- granulocyte and maerophage (CFU-GM) was investigated.Results The drug content of CTS-Pasp-5FU was 40.2% and the encapsulation efficiency was 34.9%.Compared with normal saline group,tumor volume of 5-FU group and CTS-pasp-5-FU group were significantly decreased 21 days after treatment (P

To promote bilingual teaching reform and explore a proper bilingual teaching mode in China,we studied the bilingual teaching of the course “Biochemistry on special subjects”.The present paper mainly designs and discusses the object of the course,teaching materials,contents and methods as well as the building of feedback and evaluation system of the course.

Objective To investigate the advantages of detection for EGFR gene mutations by denaturing high performance liquid chromatography (DHPLC) technology. Methods DHPLC was used to detect EGFR gene mutations at exon 19 and 21 in 49 cases of non-small cell lung cancer (NSCLC) patients,and the direct DNA sequencing was used to verify the accuracy of DHPLC detection. Results EGFR gene mutation was identified from 13 of 49 cases by DHPLC,including deletion mutation at exon 19 in 10 cases (76.92 %) and alternative mutations at exon 21 in 3 cases (23.08 %). Mutation results of DHPLC was consistent with DNA direct sequencing. The results of the direct DNA sequencing were the same as those of DHPLC. The sensitivity of mutation test by DHPLC was 100 %. Conclusion DHPLC technology can be used for large scale screening of EGFR gene mutation with rapid and accuracy.

OBJECTIVETo study the molecular mechanism of Yangjing Zhongyu Decoction (YZD) n-butanol extracts (ZDC) and ethyl acetate extracts (YSYZ) in reducing androgen in porcine granulose cells by mitogen-activated protein kinase (MAPK) pathway.

METHODSPorcine granulose cells were isolated and cultured. They were inoculated by MAPK inhibitor PD98059 at different concentrations, and then they were divided into the blank control group (0), 1, 3, 10, and 25 micromol/L groups. After 24-h culture the cytochrome P450c17a (CYP17) mRNA expression level was detected using Real-time fluorescent quantitative PCR. Contents of androgen (testosterone) in the supernate were detected using RIA and optimal PD98059 concentration screened. After intervened by 10 micromol/L PD98059 for 24 h, the culture solution was intervened by effective ingredients of with or without YZD or YSYZ at various concentrations (0, 1 , 5, 25, 50 mg/mL) at various time points (3, 6, 18, 24 h). Expression levels of p-ERK1/2, c-Fos and CYP17 were detected by Western blot. Testosterone content in the supernate was determined by radioimmunoassay (RIA).

RESULTSTen pLmol/L PD98059 could obviously decrease p-ERK1/2 protein expression and increase CYP17 mRMA expression, and elevate testosterone content in the supernate (P < 0.05). ZDC and YSYZ at 25 ng/mL could increase p-ERK1/2 protein expression and c-Fos levels, and reduce CYP17 protein expression, and lower testosterone content in the supernate after 6-h intervention (P < 0.01).

CONCLUSIONEffective ingredients of YZD could reduce androgen production in porcine granulose cells through increasing activities of MAPK.

Androgens ; Animals ; Drugs, Chinese Herbal ; pharmacology ; Female ; Flavonoids ; Granulosa Cells ; metabolism ; Mitogen-Activated Protein Kinase 3 ; metabolism ; Mitogen-Activated Protein Kinases ; metabolism ; RNA, Messenger ; Swine

Objective:To observe the effects of ubenimex adjuvant chemotherapy on adverse reactions, quality of life, inflammatory cytokines and immune function in patients with breast cancer.Methods:A total of 102 breast cancer patients treated in Hangzhou Cancer Hospital from January 2016 to December 2018 were selected in the research, and they were divided into observation group and control group according to the random number table method, with 51 cases in each group.The control group was given chemotherapy alone, and the observation group was treated with ubenimex on the basis of the control group.The short-term clinical efficacy, immune function indicators (CD 3+, CD 4+, CD 8+, CD 4+/CD 8+), serum inflammatory factors[interleukin-10 (IL-10), tumor necrosis factor α (TNF-α), C-reactive protein (CRP)] and quality of life[Quality of Life Questionnaire-Core 30 (QLQ-C30)] before treatment and after 3 months of treatment were compared between the two groups, and the occurrence of adverse reactions were recorded during treatment. Results:After treatment, the total effective rate in the observation group was higher than that in the control group (64.71% vs.45.10%) (χ 2=3.960, P<0.05). After 3 months of treatment, the CD 3+, CD 4+ and CD 4+/CD 8+ levels in the observation group were significantly higher than those before treatment[(60.26±3.18)%, (43.17±2.35)%, (1.59±0.11) vs.(52.25±3.15)%, (37.36±4.24)%, (1.11±0.19)]( t=18.074, 12.592, 22.853, all P<0.05), which were significantly higher than those in the control group [(46.58±2.19)%, (33.26±2.28)%, (0.99±0.10)] ( t=25.302, 21.614, 28.823, all P<0.05). The CD 8+ level in the observation group was significantly lower than that before treatment[(27.16±2.14)% vs.(33.59±2.54)%]( t=19.624, P<0.05), which was significantly lower than that in the control group [(33.57±2.24)%] ( t=14.776, P<0.05). The CD 3+, CD 4+ and CD 4+/CD 8+ levels in the control group were significantly lower than those before treatment[(46.58±2.19)%, (33.26±2.28)%, (0.99±0.10) vs.(52.25±3.34)%, (37.16±3.09)%, (1.11±0.12)]( t=14.644, 10.373, 7.791, all P<0.05), and there was no statistically significant difference in the CD 8+ level compared with before treatment[(33.57±2.24)% vs.(33.49±2.19)%]( P>0.05). After 3 months of treatment, the IL-10 level in the observation group was significantly higher than that before treatment[(7.85±1.08)pg/L vs.(5.26±1.48)pg/L]( t=14.450, P<0.05), while the TNF-α and CRP levels were significantly lower than those before treatment[(5.26±1.59)ng/L, (9.63±1.55)mg/L vs.(10.16±2.12)ng/L, (43.26±6.06)mg/L] ( t=18.864, 63.119, all P<0.05), but there were no statistically significant differences in the levels of IL-10, TNF-α and CRP in control group compared with before treatment[(5.26±1.34)pg/L, (9.58±2.49)ng/L, (44.58±7.76)mg/L vs.(5.16±1.59)pg/L, (10.06±2.65)ng/L, (43.07±6.09)mg/L]( t=0.487, 1.334, 1.557, all P>0.05). After 3 months of treatment, the scores of physical function, role function, emotional function, cognitive function and social function in the observation group were significantly higher than those before treatment[(70.46±7.42)points, (74.97±7.55)points, (72.13±8.25)points, (63.43±6.75)points, (69.24±5.91)points vs.(50.42±7.95)points, (55.14±6.31)points, (55.15±7.31)points, (48.21±8.33)points, (48.55±5.73)points]( t=18.623, 20.435, 15.586, 14.415, 21.440, all P<0.05), and the above scores in the control group were significantly higher than those before treatment[(63.17±7.01)points, (67.10±7.22)points, (64.41±7.95)points, (52.93±8.14)points, (60.51±8.34)points vs.(51.43±6.65)points, (55.34±6.66)points, (55.15±7.64)points, (49.13±8.41)points, (47.13±7.45)points]( t=12.275, 12.101, 8.484, 3.279, 12.103, all P<0.05), and the scores in the observation group were significantly higher than those in the control group ( t=5.100, 5.380, 4.812, 7.091, 6.099, all P<0.05). The total incidence of adverse reactions in the observation group was significantly lower than that in the control group (11.76% vs.35.29%) (χ 2=7.846, P<0.05). Conclusion:Adjuvant chemotherapy with ubenimex can significantly improve the immune function of breast cancer patients, enhance the quality of life, and can significantly reduce the levels of inflammatory factors and adverse reactions during chemotherapy, and it is safe and effective.