2.Application of antibody cocktail method in the immunohistochemistry.
Hong YANG ; Ke LI ; Dan-dan DONG
Chinese Journal of Pathology 2005;34(3):182-183
Actins
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immunology
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metabolism
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Antibodies, Monoclonal
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analysis
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Antigens, CD34
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immunology
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metabolism
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Breast Neoplasms
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metabolism
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Female
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Humans
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Immunohistochemistry
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methods
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Lung Neoplasms
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metabolism
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Membrane Proteins
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immunology
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metabolism
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Tumor Suppressor Protein p53
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immunology
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metabolism
3.Influence of sensation of uncertainty in illness on curative effect of two-week rehabilitation program in patients with acute myocardial infarction
Dan DONG ; Yang LIU ; Lijie ZHANG
Chinese Journal of cardiovascular Rehabilitation Medicine 2012;21(5):461-465
Objective: To observe influence of sensation of uncertainty in illness on curative effect of two-week rehabilitation program in patients with acute myocardial infarction (AMI). Methods: A total of 85 AMI patients recently treated in our hospital were selected continuously. They received two-week rehabilitation program treatment and were assessed by Missals uncertainty in illness scale (MUIS). According to MUIS scores, AMI patients were divided into middle-high score group (74.8~117.4 scores, n=51) and low score group (32~74.7 scores, n=34), and they were compared with 43 patients with coronary heart disease (CHD) angina pectoris (CHD control group) received treatment in the same period. Results: Compared with CHD control group, there were significant increase in each dimension score and total score of MUIS [total score (60.61±12.42) scores vs. (78.34±15.20) scores]in AMI patients (P<0.05~0.01). The exercise peak heart rate of MUIS middle-high score group was significantly higher than that of low score group [(137.80±26.49) times/min vs. (126.12±20.51) times/min, P<0.01]. Compared with low score group, there were significant decrease in total scores of activity of daily living (ADL) scale [(84.15±16.38) scores vs. (73.92±14.21) scores] and the 36-item short-form general heath survey [SF-36, (45.22±6.86) scores vs. (37.95±6.43) scores], and significant increase in total score of symptom checklist (SCL)-90 [(138.35±36.47) scores vs. (151.87±42.61) scores], mean days in CCU [(2.53±0.26)d vs. (2.77±0.29)d], mean days on bed [(4.46±0.25)d vs. (5.38±1.22)d], mean hospital day [(20.48±3.16)d vs. (25.37±3.82)d] and mean inpatient fee [(39.1±8.2) thousand RMB vs. (45.7±9.3) thousand RMB] in middle-high score group, P<0.05~0.01. Conclusion: There is significant sensation of uncertainty in illness in patients with acute myocardial infarction, and it makes curative effect of two-week rehabilitation program significantly decrease.
4.Isoflavonoids from Caragana changduensis and their nitric oxideinhibitory activities.
Xiao-dong SUN ; Shi-ming FANG ; Mao-dan ZANG ; Cheng-xiong YANG ; He-ran LI ; Susumu KITANAKA ; Xue-dong YANG
China Journal of Chinese Materia Medica 2015;40(16):3220-3223
Ten isoflavonoids were isolated from the heartwoods of Caragana changduensis Lion f. by means of various column chromatographic techniques. Based on the detailed spectral data analysis (MS and NMR), as well as comparison with the literatures, their chemical structures were determined as 7,2'-dihydroxy-8,4'-dimethoxyisoflavone (1), 4'-hydroxy-7,3'-dimethoxyisoflavone (2), 5, 7, 4'-trihydroxy-2',5'-dimethoxyisoflavone (3), prunetin (4), afrormosin (5), odoratin (6), genistein (7), texasin (8), pratensein (9), and 6,7,3'-trihydroxy-4'-methoxyisoflavone (10). Among them, compounds 1-3 and 9-10 were isolated from the Caragana genus for the first time. All the compounds were obtained from this species for the first time. In the preliminary assays, compounds 1, 2, 6, and 7 possessed significant inhibitory effects on NO production, with IC50 values of 48.12, 25.32, 62.71, 43.59 μmol x L(-1), respectively.
Animals
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Caragana
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chemistry
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Drugs, Chinese Herbal
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chemistry
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isolation & purification
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pharmacology
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Isoflavones
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chemistry
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isolation & purification
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pharmacology
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Macrophages
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drug effects
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metabolism
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Mice
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Molecular Structure
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Nitric Oxide
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antagonists & inhibitors
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metabolism
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RAW 264.7 Cells
5.3.0T MRI Multi-b-value Diffusion Weighted Imaging in the Differential Diagnosis of Female Pelvic Benign and Malignant Lesions
Minxia QIAO ; Huiping SHI ; Dan QIN ; Xujia ZHOU ; Shibo DONG ; Fan YANG ; Peng LIANG
Chinese Journal of Medical Imaging 2013;(12):951-954
Purpose To explore the diagnostic value of double exponential model for pelvic lesions using 3.0T MRI for the diagnosis of pelvic lesion. Materials and Methods Fifty patients with pelvic lesions (30 benign cases and 20 malignant cases) underwent MR750-diffusion weighted imaging (DWI) scans, with b values of 0, 50, 300, 600, 800 and 1200 s/mm2, Functool-MADC software was used on AW 451 workstations for data processing, Slow ADC value, Fast ADC value, Standard ADC value, Fraction of fast ADC value were recorded and compared between benign and malignant lesions, and Standard ADC images were fused with axial T2 fat-suppressed images. Results Slow ADC values [(1.83±0.86)×10-3 mm2/s] and Standard ADC values [(1.79±0.78)×10-3 mm2/s] of benign lesions were larger than those of the malignant lesions [Slow ADC values:(1.05±0.31)×10-3 mm2/s;Standard ADC values:(1.13±0.39)×10-3 mm2/s] (t=3.90, 3.51;P<0.01), and the difference of Slow ADC value was largest between benign and malignant lesions. Slow ADC values of both benign and malignant lesions were significantly less than the Fast ADC values [benign:Slow ADC value=(1.83±0.86)×10-3 mm2/s, Fast ADC value=(16.95±8.63)×10-3 mm2/s; malignant: Slow ADC value=(1.05±0.31)×10-3 mm2/s, Fast ADC value=(15.12±9.90)×10-3 mm2/s] (t=-10.40,-6.29;P<0.01). Conclusion Double exponential decay model is capable of differentiating benign and malignant pelvic tumors, thus is of great significance for clinical preoperative diagnosis.
6.Dermis-derived cell subpopulation is used to repair mouse calvarial defects
Tingliang WANG ; Jinguang HE ; Yang ZHANG ; Dan LI ; Jiasheng DONG ; Lian ZHU
Chinese Journal of Tissue Engineering Research 2015;19(19):3067-3073
BACKGROUND:In consideration of skin as the largest organ al over the body and its abundant vessels and vessel plexuses, there would be sufficient adult stem cels for tissue engineering. OBJECTIVE:To investigate the osteogenic potential of dermis-derived bone morphogenetic protein receptor subtype IB (BMPR-IB) positive cels. METHODS:In current study, histochemical analysis was adopted to study the localization and expression of BMPR-IB+ cels in skin. Fresh skin samples were digested into single cel suspension. Then, the surface marker BMPR-IB was used to isolate cel subpopulation by magnetic activated cel sorting from freshly prepared single cel suspension. After that, the osteogenic potential in vitro andin vivo was tested. Alkaline phosphatase staining and alizarin red staining were performed after osteogenic inductionin vitro. The BMPR-IB+ cels were seeded onto coral scaffolds, and the scaffolds were used to repair critical-sized calvarial defects of mice. Histochemical analysis was performed at 6 weeks postoperatively and micro-CT analysis was carried out at 24 weeks postoperatively to evaluate the ability of bone repairment. RESULTS AND CONCLUSION:We localized BMPR-IB cels in situ by immunohistochemistry that turned out to be expressed in the reticular layer of dermis and by single cels. Cel subpopulation which expressed BMPR-IB could be sorted by magnetic activated cel sorting. Alkaline phosphatase staining was obviously positive and lots of calcium modules were confirmed by alizarin red staining after osteogenic induction, indicating that BMPR-IB+ cels had the osteogenic potentialin vitro. Histochemical analysis demonstrated that plenty of new bone formation was found in BMPR-IB+ cels group after 6 weeks in vivo. Micro-CT analysis revealed that BMPR-IB+ cels-coral scaffold complex could repair calvarial defects successfuly after 24 weeksin vivo. These results indicated that dermis-derived BMPR-IB+ cels possessed adequate osteogenic potential. Moreover, they might be promising seed cels for bone tissue engineering.
7.Hemostatic effect and mechanism of a non-polysaccharide fraction of Bletilla striata
Feifei ZHAO ; Xin YANG ; Dan XU ; Li DONG ; Jing LI ; Yonglin WANG ; Shanggao LIAO
Chinese Pharmacological Bulletin 2016;32(8):1121-1126
Aim To understand the hemostatic effect of a non-polysaccharide fraction of Bletilla striata ( BS-80EE) and to clarify its mechanism of action .Methods The non-polysaccharide fraction ( BS-80 EE ) was prepared by passing the 95%ethanol extract of Bletilla striata through a D101 macroporous resin column elu-ted first with water and then with 80%ethanol.Bleed-ing time ( BT ) and clotting time ( CT ) of heparinized mice were employed as indicators for evaluating the he-mostatic effect of BS-80EE.The mechanism of action was investigated through observing the effect of BS-80 EE on platelet aggregation induced by adenosine diphosphate ( ADP) in rats with nephelometry and tes-ting the effect of BS-80EE on the thrombin time(TT), prothrombin time(PT), activated partial thromboplas-tin time(APTT), fibrinogen(FIB), P-selectin(P-S), thrombin-antithrombin complex ( TAT ) , D-dimer ( D-D) and plasminogen activator inhibitor-1 ( PAI-1 ) . Results BS-80 EE significantly shortened the CT and BT( P<0.01 or 0.05 ) of heparin mice in a dose-de-pendent manner; groups of all doses significantly re-duced the rat TT ( P <0.01 or 0.05 ) , and the high-dose group significantly increased the FIB content ( P<0.05); the mid-dose group and high-dose groups of BS-80EE significantly increased the contents of P-S, TAT and PAI-1 , while reduced the D-D production in rats ( P <0.01 ); although dose-dependent reductions of APTT and PT were observed for each treatment-group, no significance was observed .Conclusion BS-80EE possess pronounced hemostatic effect by promo-ting platelet aggregation and coagulation .
8.Intervention Effect of Modified Dachengqi Decoction on Intestinal Mucosal Barrier of Severe Acute Pancreatitis Model Rats.
Dan-ping QIN ; Xia WEI ; Guo-dong FANG ; Feng YANG ; Deng-pan LAI
Chinese Journal of Integrated Traditional and Western Medicine 2015;35(12):1482-1489
OBJECTIVETo study the effect of Modified Dachengqi Decoction (MDD) as whole course therapy on mediators of inflammation in severe acute pancreatitis (SAP) model rats, and to compare interventional advantages over intestinal mucosal barrier (IMB) of SAP rats between whole course therapy of MDD and early stage therapy of MDD.
METHODSTotally 190 SD rats were divided into five groups according to random digit table, i.e., the sham-operation group, the model group, the octreotide (OT) group, the early stage MDD treatment group, the whole course MDD treatment group, 38 in each group. SAP models were established with retrograde injection of 5% sodium taurocholate into the pancreaticobiliary duct. Three hours after modeling normal saline (NS) was administered to rats in the sham-operation group and the model group by gastrogavage, once per 12 h.1.35 µg/100 g OT was subcutaneously injected to rats in the OT group, once every 8 h. 0.4 mL/100 g MDD was administered to rats in the early stage MDD treatment group, and 6 h later changed to NS (once per 12 h).0.4 mL/100 g MDD was administered to rats in the whole course MDD treatment group, once every 12 h. The accumulative survival rate and morphological manifestations of pancreas and small intestine were observed under microscope 48 h after modeling. Pathologic scores of the pancreas and small intestine were conducted at 4, 6, 24, and 48 h after modeling. Contents of serum amylase (AMY), alanine transaminase (ALT), and TNF-α were also detected. The expression of high mobility group box protein 1 (HMGB1) in the small intestine tissue was also detected by Western blot. The positive rate of bacterial translocation in mesenteric lymph nodes (MLNs) was observed within 48 h. Correlations between serum TNF-α or HMGB1 in small intestinal tissue and pathological scores of the pancreas or the small intestine were analyzed.
RESULTSThe accumulative survival rate was 100. 0% in the sham-operation group, 79. 2% in the whole course MDD treatment group, 70. 8% in the OT group, 45. 8% in the early stage MDD treatment group, and 37.5% in the model group. At 6 h after modeling, pathological scores decreased more in the whole course MDD treatment group, the early stage MDD treatment group, the OT group than in the model group (P < 0.05). At 24 and 48 h after modeling, pathological scores of the pancreas and the small intestine decreased more in the whole course MDD treatment group and the OT group than in the early stage MDD treatment group (P <0. 05). At 6, 24, and 48 h after modeling, serum contents of AMY and ALT both decreased more in the whole course MDD treatment group, the early stage MDD treatment group, the OT group than in the model group (P < 0.05). At 48 h after modeling serum contents of AMY and ALT both decreased more in the whole course MDD treatment group and the OT group than in the early stage MDD treatment group (P < 0.05). At 6 h after modeling serum TNF-α levels decreased more in the whole course MDD treatment group, the early stage MDD treatment group, the OT group than in the model group (P < 0.05). At 6, 24, and 48 h after modeling the level of HMGB1 in the small intestinal tissue decreased more in the whole course MDD treatment group, the early stage MDD treatment group, the OT group than in the model group (P < 0.05). Of them, HMGB1 levels at 24 and 48 h were lower in the whole course MDD treatment group and the OT group than in the early stage MDD treatment group (P < 0.05). The number of MLNs bacterial translocation at 48 h after modeling was lower in the whole course MDD treatment group and the OT group than in the early stage MDD treatment group and the model group (P < 0.05). Serum TNF-α contents within 6 h were positively correlated with pathological scores of pancreas (r = 0.579, P < 0.01). ROC curve showed that serum TNF-α contents could predict the severity of SAP (ROC = 0.990, 95% Cl: 0.971 to 1.000). HMGB1 in the small intestine was positively correlated with pathological scores of the small intestine (r = 0.620, P < 0.01).
CONCLUSIONSEarly stage use of MDD could effectively reduce the release of TNF-α, while whole course use of MDD could effectively inhibit the expression of HMGB1. The latter could preferably attenuate injuries of the pancreas and the small intestine, lower MLNs bacterial translocation, and elevate the survival rate.
Animals ; Bacterial Translocation ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; HMGB1 Protein ; Intestinal Mucosa ; drug effects ; Octreotide ; Pancreas ; Pancreatitis ; drug therapy ; Plant Extracts ; pharmacology ; therapeutic use ; Rats ; Rats, Sprague-Dawley ; Taurocholic Acid ; Tumor Necrosis Factor-alpha
9.Ultrasonographic Evaluation of Infundibular Bladder Neck Formation in Female Stress Urinary Incontinence
Lan BU ; Fang NIE ; Dan YANG ; Yan CHE ; Tiantian DONG ; Hong PAN
Chinese Journal of Medical Imaging 2017;25(7):547-549,554
Purpose To investigate the relationship of infundibular bladder neck formation with pelvic floor support structure injury and urethral sphincter defect and its significance in female stress urinary incontinence.Materials and Methods The pelvic floor images of seventy-four female patients with stress urinary incontinence treated in the outpatient Department of Lanzhou University Second Hospital from April 2015 to August 2016 were analyzed retrospectively.The location of the bladder neck,posterior vesicourethral angle and the infundibular bladder neck formation were observed by the transperineal ultrasound under the resting state and the maximum Valsalva status.Meanwhile the thickness of middle urethral sphincter was measured under resting state.At the same time,eighty-one women visiting our hospital for regular physical examination were enrolled as control group.Results The infundibular urinary bladder neck formation rate (66.2%) in the stress urinary incontinence group was significantly higher than that in the control group (4.9%) under maximum Valsalva state,the difference was statistically significant (P<0.05).The extent of the bladder neck descending and posterior vesicourethral angle in the stress urinary incontinence group were notably higher than those in the control group,both of the difference was statistically significant (P<0.05).Stress urinary incontinence was confirmed with urethral sphincter defect by urodynamics in nine patients,in whom the infundibular bladder neck occurred.The thickness of the middle urethral sphincter in these nine patients showed no obvious difference with that in patients without sphincter defect and subjects in normal control group (P>0.05).Conclusion The infundibular bladder neck formation,which is closely related to the pelvic floor support structure dysfunction and urethral sphincter defect,is an important indication of stress urinary incontinence.However,the assessment of urethral sphincter defect through urethral sphincter thickness need to be further studied.
10.Echinococcus granulosus stimulates high PPARα/γ expression and drives polarization of macrophages in vitro
Congzhe CHEN ; Dan DONG ; Hairui FANG ; Hongqun JIANG ; Jun HOU ; Kun YANG ; Feng GUO ; Xueling CHEN
Chinese Journal of Immunology 2017;33(1):16-19,24
Objective:To investigate the expression levels of PPARα/γin RAW264. 7 cells in the early stages of co-cultivation with Echinococcus granulosus in vitro. Methods:RAW264. 7 cells were co-cultured with E. granulosus and collected at 12,24,36,48, 72 h. The mRNA levels of PPAR-γ,PPAR-α,M1 macrophages-associated cytokines including TNF-α,MCP-1 and IL-1β,and M2 mac-rophages-associated cytokines including Arg-1,TGF-β and Fizz-1 were detected by qRT-PCR. The protein levels of Arg-1 and MR were analyzed by ELISA. Results:The expression levels of PPAR-γ, PPAR-αand the M2 macrophages-associated cytokines including Arg-1,TGF-β,Fizz-1 and MR were significantly increased,especially at 72 h (P<0. 05). M1 macrophages-associated cytokines including TNF-α,MCP-1 and IL-1β were decreased at 72h although increased at first. Conclusion:During the early stages of co-cultivation with Echinococcus granulosus in vitro, the levels of PPAR-γ/α are up-regulated in RAW264. 7 cells, which may drive macrophage polarization and play a role in the immune escape.