ATP Binding Cassette Transporter, Sub-Family B ; metabolism ; Animals ; Anticonvulsants ; pharmacology ; Brain ; drug effects ; metabolism ; Rats

0.05).The third day after SE,the expressions of MVP in CA1 and CA3 in adult rats experiment group increased,and in CA3,the value reached up to(4.0?1.41)in adult experiment group,which had significant differences compared with control groups(P

ATP-Binding Cassette, Sub-Family B, Member 1 ; biosynthesis ; Animals ; Brain ; metabolism ; Disease Models, Animal ; Glycoproteins ; metabolism ; Male ; Rats ; Rats, Sprague-Dawley ; Status Epilepticus ; metabolism

[Objective] To observe the effect of Hewei Jiangni Decoction (HJD) in preventing and treating stress ulcer in rats and to explore its therapeutic mechanism. [Methods] Sixty SD rats were randomized into groups A (model), B (ranitidine 0.05 g/kg), C (HJD 1.25 g/kg), D (HJD 2.5 g/kg) and E (HJD 5.0 g/kg). Rat models of stress ulcer were induced by water-immersion method. Model group was given normal saline and the rats in other groups were medicated by gavage for 5 days. After treatment, effects of HJD on the incidence of stress ulcer, volume and pH value of gastric juice, prostaglandin E2 (PGE2) and nitric oxide (NO) contents in gastric mucosa were detected. [Results] HJD decreased ulcer index, gastric secretion and NO content and increased the content of PGE2 ( P

[Objective] To observe the effect of Hewei Jiangni Decoction (HJD) for the treatment of stress ulcer and to explore its possible mechanism. [Methods] Sixty SD rats were randomized into 6 groups: normal, model, ranitidine (0.05 g?kg-1?d-1), and HJD groups (1.25, 2,50 and5.00g?kg-1?d-1 respectively). Except the normal group, the rats in other groups were treated with water immersion method to induce stress ulcer. Then the drugs according to the experimental design were administered to ranitidine and HJD groups for 5 days successively , and the model and normal groups were given saline. Gastric mucosal blood flow was detected by laser Doppler flowmeter, and inducible nitric oxide synthetase (iNOS) mRNA expression in gastric mucosa was examined by reverse transcriptase polymerase chain reaction (RT-PCR) . [Results] HJD decreased ulcer index, increased gastric mucosal blood flow (GMBF), and reduced iNOS mRNA expression in the gastric mucosa, the difference being significant ( P

Objective To observe the effects of Angong Niuhuang Bolus(ANB) on vital organ injury and mortality in rats with sepsis.Methods SD rats were randomized into normal control group,model group,and low-,middle-and high-dose ANB groups(in the dose of 1.0,2.0 and 3.0 g?kg-1?d-1 respectively).Except that the normal control group,the rats in other groups received cecal ligation and puncture(CLP) method to induce sepsis through abdominal infection.ANB groups were given gastric gavage of ANB 0.5 hours before CLP and 6 hours after CLP.After CLP for 24 hours,the mortality was observed,serum levels of alanine aminotransferase(ALT),total bilirubin(TB) and creatinine were detected,and pulmonary myeloperoxidase(MPO) activity in all of the rats was examined.Results Serum ALT and TB levels,MAO activity and 24-hour mortality were increased in the model group(P

Objective To investigate the effect of Angong Niuhuang Bolus(ANB) on mRNA expression of high mobility group box-1(HMGB1) protein and myeloperoxidase activity in the lung of rats with sepsis induced by cecal ligation and puncture(CLP) method.Methods Seventy-five male SD rats were randomly divided into normal control group,model group,AG490(a Janusk kinase inhibitor) treatment group(subcutaneous injection of AG490 8mg/kg 0.5 h before CLP),rapamycin(RPM,a signal transduction and transcription activator inhibitor) treatment group(subcutaneous injection of RPM 0.4 mg/kg 0.5 h before CLP),and low-,middle-and high-dose ANB groups(gastric gavage of ANB in the dose of 1.0,2.0 and 3.0 g?kg-1?d-1 respectively).The animals were sacrificed 24h after CLP,and then lung tissue samples were collected to determine the expression of HMGB1 mRNA by the method of reverse transcription polymerase chain reaction(RT-PCR).Meanwhile,the activity of pulmonary myeloperoxidase(MPO) was also measured.Results Compared with the normal control group,the mRNA expression of HMGB1 was significantly enhanced(P

OBJECTIVETo observe the protective effect of active fractions of Huanglian Jiedu Decoction (HJD) on primary cortical neuron injury after oxygen-glucose deprivation (OGD)/reperfusion (R) injury. Methods Using macroporous resin method, HJDFE30, HJDFE50, HJDFE75, and HJDFE95 with 30%, 50%, 75%, and 95% alcohol were respectively prepared. Then the content of active components in different HJD fractions was determined with reverse phase high-performance liquid chromatography (RP-HPLC). The OGD/R injury model was induced by sodium dithionite on primary cortical neurons in neonate rats. MTT assay was used to observe the effect of four fractions (HJDFE30, HJDFE50, HJDFE75, and HJDFE95) and seven index components of HJD on the neuron viability.

RESULTSRP-HPLC showed active component(s) contained in HJDFE30 was geniposide; baicalin, palmatine, berberine, and wogonside contained in HJDFE50; baicalin, berberine, baicalein, and wogonin contained in HJDFE75. The neuron viability was decreased after OGD for 20 min and reperfusion for 1 h, (P <0. 01), and significantly increased after administered with HJD, HJDFE30, HJDFE50, and HJDFE75 (P <0. 05, P <0. 01). Geniposide, baicalin, baicalein, palmatine, wogonside, and wogonin could increase the cortical neuron viability (P <0. 05, P <0. 01).

CONCLUSIONSHJDFE30, HJDFE50, and HJDFE75, as active fractions of HJD, had protective effect on primary cortical neuron injury after OGD/R. Furthermore, geniposide, baicalin, and baicalein were main active components of HJD.

Animals ; Berberine ; Berberine Alkaloids ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Flavanones ; Flavonoids ; Glucose ; metabolism ; Iridoids ; Models, Animal ; Neurons ; Oxygen ; metabolism ; Rats ; Reperfusion Injury ; drug therapy

Objective To investigate the effect of Octreotide on pancreatic head carcinoma patients with obstructive jaundice who underwent endoscopic retrograde cholangiopancreatography (ERCP) with pancreatic stent placement.Methods Niney-nine patients hospitalized in Department of Gastroenterology of Wuhan Central Hospital from Jan 2006 to Dec 2011 were included in this study.All the patients were diagnosed as pancreatic head carcinoma with obstructive jaundice.The patients were randomly divided into the Octreotide treatment group and the control group.Both groups underwent ERCP with pancreatic duct stent placement for malignant biliary obstruction.The patients in Octreotide treatment group were injected with subcutaneous Octreotide at a dose of 0.1 mg twice per day for more than 90 day till death.The changes of serum total bilirubin before and after treatment were compared.The improvement of symptoms of nausea,vomiting,abdominal pain,diarrhea and anorexia was compared.The complication rates and survival were also determined.Results Postoperative recurrence of jaundice was observed in six patients in control group,and the cause may be stent occlusion,and 3 of the 6 patients underwent a second ERCP and stent placement,then jaundice was relieved,the other 3 patients did not receive a second ERCP.The serum bilirubin level in the remaining 45 patients returned to basically normal value (below 2 times of the normal value).The prevalence of nausea,vomiting,abdominal pain,diarrhea and anorexia in the 2 groups was not statistically different before treatment,and after treatment the prevalence of symptoms in the 2 groups was significantly decreased except for diarrhea.The decrease in Octreotide treatment group was more obvious than that in control group,and the difference between the two groups was statistically significant (P ＜ 0.01).In the control group,post-ERCP pancreatitis occurred in three patients,and all were cured after treatment.There was no post-ERCP pancreatitis occurred in the Octreotide treatment group.Minor pain at the injection site was noted in three patients in the Octreotide treatment group.Pain was relieved after changing the injection site.The survival was significantly longer in the Octreotide group than that in control group [(14.4 ± 8.7) months vs (7.3 ± 5.3) months,P ＜ 0.05),and the difference between the two groups was statistically significant (P ＜ 0.05).Conclusions Octreotide can improve the quality of life and increase the survival of patients with pancreatic head carcinoma who undergo ERCP with pancreatic duct stent placement.

OBJECTIVETo study the effects of valproate acid (VPA) on serum lipid and leptin levels and cerebral cortex in juvenile and adult rats.

METHODSTwenty healthy juvenile female Sprague-Dawley (SD) rats (21-day-old) and twenty healthy adult female SD rats (2-month-old) were randomly divided into four groups (n=10 each): juvenile control, juvenile VPA, adult control and adult VPA. Juvenile and adult VPA groups were fed with VPA 200 mg/kg daily, while the two control groups were fed with normal saline. The body weights were recorded weekly. Six weeks after feeding, serum and brain samples were obtained. Serum lipid levels including total cholesterol (TC), triglycerides (TG) and lower density lipoprotein cholesterol (LDL-C) were determined. Serum leptin (LEP) levels were measured by radioimmunoassay (RIA). Myelin staining and Nissl staining were used to evaluate the changes of brain tissues.

RESULTSThe weight and serum LEP and lipid levels in both juvenile and adult VPA groups increased significantly compared with those in the control groups (P<0.05). The juvenile VPA group had more increased serum LEP and lipid levels than the adult VPA group (P<0.05). The Myelin staining showed that the average fiber density in the VPA groups was significantly lower than that in the control groups (P<0.05). The Nissl staining showed that the number of toluidine blue staining neurons in the VPA groups was not statistically different from the control groups.

CONCLUSIONSVPA may increase serum LEP and lipid levels in both juvenile and adult rats, and more increased levels may be found in juvenile rats. Long-term VPA treatment may have an adverse effect on brain myelination, but no effect on neurons.

Animals ; Anticonvulsants ; toxicity ; Body Weight ; drug effects ; Cerebral Cortex ; drug effects ; pathology ; Female ; Leptin ; blood ; Lipids ; blood ; Myelin Sheath ; drug effects ; pathology ; Rats ; Rats, Sprague-Dawley ; Valproic Acid ; toxicity