1.A meta-analysis on transcranial magnetic stimulation for Parkinson's disease
Dan WANG ; Ping GU ; Junxia ZHAI
Chinese Journal of Physical Medicine and Rehabilitation 2010;32(8):596-601
Objective To systematically review findings on the effectiveness and safety of transcranial magnetic stimulation (TMS) for treating Parkinson's disease. Methods Foreign and Chinese databases were searched to find relevant trials. The searches were supplemented by searching the reference lists of the published trials. The studies were separated into two groups: those applying TMS at frequencies higher than 1 Hz, and those at 1 Hz or lower. Meta-analysis was performed with the aid of RevMan 5.0 software. Results Both low-frequency and highfrequency TMS can significantly reduce total scores on the Unified Parkinson's Disease Rating Scale (UPDRS) compared with sham stimulations. High-frequency TMS can significantly reduce UPDRS motor scores compared with sham stimulations. On the other hand, low-frequency TMS studies showed no significant overall improvement in motor scores, mental scores or in the activities of daily living (ADL) UPDRS scores. Low-frequency and high-frequency TMS studies also showed no significant overall improvement in Schwab and England ADL scores. Conclusions TMS therapy is safe and partially effective for treating Parkinson's disease.
2.Quantitative analysis of seven phenolic acids in eight Yinqiao Jiedu serial preparations by quantitative analysis of multi-components with single-marker.
Jun-jun WANG ; Li ZHANG ; Qing GUO ; Jun-ping KOU ; Bo-yang YU ; Dan-hua GU
Acta Pharmaceutica Sinica 2015;50(4):480-485
The study aims to develop a unified method to determine seven phenolic acids (neochlorogenic acid, chlorogenic acid, 4-caffeoylquinic acid, caffeic acid, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C) contained in honeysuckle flower that is the monarch drug of all the eight Yinqiao Jiedu serial preparations using quantitative analysis of multi-components by single-marker (QAMS). Firstly, chlorogenic acid was used as a reference to get the average relative correction factors (RCFs) of the other phenolic acids in ratios to the reference; columns and instruments from different companies were used to validate the durability of the achieved RCFs in different levels of standard solutions; and honeysuckle flower extract was used as the reference substance to fix the positions of chromatographic peaks. Secondly, the contents of seven phenolic acids in eight different Yinqiao Jiedu serial preparations samples were calculated based on the RCFs durability. Finally, the quantitative results were compared between QAMS and the external standard (ES) method. The results have showed that the durability of the achieved RCFs is good (RSD during 0.80% - 2.56%), and there are no differences between the quantitative results of QAMS and ES (the relative average deviation < 0.93%). So it can be successfully used to the quantitative control of honeysuckle flower principally prescribed in Yinqiao Jiedu serial preparations.
Caffeic Acids
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analysis
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Chlorogenic Acid
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analogs & derivatives
;
analysis
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Chromatography, High Pressure Liquid
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Drugs, Chinese Herbal
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analysis
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Flowers
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chemistry
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Hydroxybenzoates
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analysis
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Lonicera
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chemistry
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Quinic Acid
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analogs & derivatives
;
analysis
3.Aspirin-PEI-beta-CyD as a novel non-viral vector for gene transfer.
Zhong-Ren WANG ; Dan CHEN ; Jun ZHOU ; Gu-Ping TANG
Journal of Zhejiang University. Medical sciences 2009;38(1):46-52
OBJECTIVETo develop a novel non-viral gene delivery vector based on PEI-beta-CyD as backbone modified with aspirin, and to identify its physicochemical characters.
METHODS1, 1-carbonyldiimidazole (CDI) was used to bind aspirin onto PEI-beta-CyD to form PEI-beta-CyD-ASP. (1)H-NMR, FT-IR, UV and XRD were used to confirm the polymer structure. The ability of condensation was demonstrated by gel retardation assay. MTT assay was used to test the cell viability in B16, Hela and A293 cell lines. Transfection efficiency of the polymer was tested in B16 cells.
RESULTThe structure of PEI-beta-CyD-ASP was confirmed by (1)H-NMR, FT-IR, UV and XRD, which efficiently condensed plasmid DNA at the N/P ratio of 4. The copolymer showed low cytotoxicity and high transfection efficiency in B16 cells.
CONCLUSIONThe synthesized aspirin-PEI-beta-CyD might be a potential gene delivery vector.
Aspirin ; chemistry ; Cell Line ; Gene Transfer Techniques ; Genetic Therapy ; methods ; Humans ; Polyethyleneimine ; chemistry ; beta-Cyclodextrins ; chemistry
4.AS-PCR assay for 20 mtDNA SNP typing and haplotype frequency.
Yan-Chai NIE ; Chen ZHANG ; Ya-Nan LIU ; Jiang-Ping HUANG ; Hai-Tao JIAO ; Dan WU ; Huai-Gu ZHOU
Journal of Forensic Medicine 2014;30(2):96-109
OBJECTIVE:
To develop a multiplex allele-specific PCR (AS-PCR) assay with three-color fluorescence labeling for mitochondrial DNA (mtDNA) SNP typing.
METHODS:
Based on the principle of AS-PCR, the primer sets were designed for 20 SNP located on the coding region of mtDNA and divided into 2 groups labeled with FAM and HEX fluorescence, respectively. A primer set included two forward (reverse) allelic specific primers with different sizes and a generic reverse (forward) primer. Blood samples from 200 unrelated individuals were analyzed by AS-PCR and capillary electrophoresis. Three random samples at least for each SNP site were examined and verified by direct sequencing. The haplotype frequency was investigated.
RESULTS:
Distinct electropherograms of 200 blood samples were obtained successfully. The typing results of direct sequencing were identical to those obtained from AS-PCR. The minimum detectable DNA concentration was 0.2 pg under the system of 10 microL. The sensitivity of the DNA concentrations ranged from 0.5 to 5 pg. The 200 individuals were assigned into 15 haplotype, and the haplotype diversity was 0.906 0.
CONCLUSION
AS-PCR is a simple, rapid and efficient method for mtDNA SNP typing, and can be applied to forensic practice.
Alleles
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DNA
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DNA Primers
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DNA, Mitochondrial/analysis*
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Electrophoresis, Capillary
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Haplotypes
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Humans
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Mitochondria
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Polymerase Chain Reaction/methods*
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Polymorphism, Single Nucleotide
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Sequence Analysis, DNA
5.Peptide TAT modified polyethylenimine-beta-cyclodextrin for gene delivery.
Li-Hua LAI ; Qi-Ying JIANG ; Dan CHEN ; Yi-Ping HU ; Hai YU ; Qing-Qing WANG ; Gu-Ping TANG
Journal of Zhejiang University. Medical sciences 2009;38(1):15-23
OBJECTIVETo develop a novel gene delivery vector TAT-PEI-beta-CyD.
METHODSbeta-cyclodextrin (beta-CyD) was linked by low molecular weight (PEI 600) via 1, 1-carbonyldiimidazole (CDI), and TAT peptide (RRRQRRKKRC) was coupled to PEI 600 by [N-succinimidy-3-(2-pyridyldithio) propionate, SPDP]. The copolymer was characterized by (1)H-NMR and FT-IR. Physiochemical characteristics of TAT-PEI-beta-CyD/DNA complexes were tested by agarose gel electrophoresis and particle size measurements. Cell viability and transfection efficiency were evaluated in A293 and B16 cells using PEI 25 kDa as a control.
RESULTTAT peptide was successfully coupled to PEI-beta-CyD. The result of gel electrophoresis showed that the TAT-PEI-beta-CyD was able to condense DNA efficiently at N/P ratio of 4. The particle size of TAT-PEI-beta-CyD/DNA complexes was around 100 nm. The cytotoxicity of TAT-PEI-beta-CyD was lower than that of PEI 25 kDa. The transfection efficiency of TAT-PEI-beta-CyD was higher than that of PEI 25 kDa in A293 and B16 cells at N/P ratio of 30.
CONCLUSIONThe novel vector TAT-PEI-beta-CyD has been developed successfully with low cytotoxicity and high transfection efficiency.
Cell Line ; Gene Transfer Techniques ; Genetic Therapy ; methods ; Humans ; Peptide Fragments ; chemistry ; Polyethyleneimine ; chemistry ; beta-Cyclodextrins ; chemistry ; tat Gene Products, Human Immunodeficiency Virus ; chemistry
6.Peptide MC10 mediated PEI-beta-CyD as a gene delivery vector targeting to Her-2 receptor.
Jun LIU ; Yi-Ping HU ; Qi-Ying JIANG ; Dan CHEN ; Hai YU ; Qing-Qing WANG ; Gu-Ping TANG
Journal of Zhejiang University. Medical sciences 2009;38(1):7-14
OBJECTIVETo develop a novel non-viral gene delivery vector based on polyethylenimine and beta-cyclodextrin targeting to Her-2 receptor (MC10-PEI-beta-CyD).
METHODSThe PEI-beta-CyD was synthesized by low molecular weight polyethylenimine (PEI, Mw 600) cross-linked beta-cyclodextrin (beta-CyD) via N, N-carbonyldiimidazole (CDI). The chemical linker[N-succinimidy-3-(2-pyridyldithio) propionate, SPDP] was used to bind peptide MC10 (MARAKEGGGC) to PEI-beta-CyD to form the vector MC10-PEI-beta-CyD. The (1)H-NMR was used to confirm the structure of vector. The DNA condensing ability,and the particle size of MC10-PEI-beta-CyD/DNA complexes were demonstrated by gel retardation assay and electron microscope observation (TEM). Cell viability was tested by MTT assay. The transfection efficiency was determined on cultured SKOV-3, A549 and MCF-7 cells.
RESULTMC10 was linked onto PEI-beta-CyD successfully. The vector was able to condense DNA at N/P ratio of 5 and particle size was about (170 +/-35)nm. The vector showed low cytotoxicity and high transfection efficiency in cultured SKOV-3, A549 and MCF-7 cells.
CONCLUSIONA novel non-viral vector MC10-PEI-beta-CyD with low cytotoxicity and high transfection efficiency has been successfully synthesized.
Cell Line ; Gene Targeting ; Gene Transfer Techniques ; Genetic Vectors ; Humans ; Peptides ; chemistry ; Polyethyleneimine ; chemistry ; pharmacology ; Receptor, ErbB-2 ; genetics ; beta-Cyclodextrins ; chemistry
7.Lentinan-graft-polyethylenimine-a novel vector for gene delivery.
Qi-Ying JIANG ; Xiao LU ; Yi-Ping HU ; Dan CHEN ; Dan-Jun ZHAO ; Jun ZHOU ; Hai YU ; Qing-Qing WANG ; Gu-Ping TANG
Journal of Zhejiang University. Medical sciences 2009;38(1):39-45
OBJECTIVETo develop a novel vector for gene delivery with low molecular weight polyethylenimine grafted to the natural polysaccharide and conjugated to folic acid (LNT-PEI-FA).
METHODSThe properties of LNT-PEI-FA were characterized by (1)H-NMR, FT-IR and TGA, respectively. The particle size of LNT-PEI-FA/DNA complex was measured. The DNA binding ability of LNT-PEI-FA was detected by gel electrophoresis retardation assay.
RESULTThe particle size of LNT-PEI-FA/DNA complex was about 200 nm. Gel electrophoresis showed that at N/P ratio of 1.8 (W/W) the polymer was able to completely condense DNA. In vitro experiments showed a high efficiency of gene transfection in A293 and B16 cell lines.
CONCLUSIONA novel non-viral vector LNT-PEI-FA was successfully synthesized and characterized, which may be applied in gene transfection research in the future.
Cell Line ; Folic Acid ; chemistry ; Gene Transfer Techniques ; Genetic Therapy ; methods ; Humans ; Lentinan ; chemistry ; Polyethyleneimine ; chemistry
8.Peptide CY11 conjugated polyethylenimine-beta-cyclodextrin for gene delivery.
Yi-Ping HU ; Qi-Ying JIANG ; Dan CHEN ; Dan-Jun ZHAO ; Xiao LU ; Hai YU ; Qing-Qing WANG ; Jun ZHOU ; Xiu-Rong HU ; Gu-Ping TANG
Journal of Zhejiang University. Medical sciences 2009;38(1):24-30
OBJECTIVETo develop a novel non-viral gene delivery vector CY11-PEI-beta-CyD and to test its gene transfection efficiency.
METHODSCY11 (CGMQLPLATWY) was conjugated to polyethylenimine-beta-cyclodextrin to form CY11-PEI-beta-CyD with a cross-linker [N-succinimidy-3-(2-pyridyldithio) propionate, SPDP]. (1)H-NMR and TGA were used to confirm the structure of vector. The DNA condensing ability of CY11-PEI-beta-CyD was investigated by gel retardation assay. Cytotoxicity of CY11-PEI-beta-CyD was determined by MTT assay and transfection efficiency was investigated in COS-7, Hela and B16 cells.
RESULTCY11 was conjugated onto PEI-beta-CyD successfully, confirmed by(1)H NMR and TGA. The novel vector effectively condensed DNA at N/P ratio of 4îIt showed low cytotoxicity up to the concentration was 160 Mgr;g/ml. The transfection efficiency was 17-fold higher than that of PEI 25 kDa at N/P ratio of 20.
CONCLUSIONThe novel vector CY11 -PEI-beta-CyD with low cytotoxic and high transfection efficiency may be used as a potential carrier for gene delivery.
Cell Line ; Gene Transfer Techniques ; Genetic Therapy ; methods ; Humans ; Peptide Fragments ; chemistry ; Polyethyleneimine ; chemistry ; Receptors, Fibroblast Growth Factor ; chemistry ; beta-Cyclodextrins ; chemistry
9.Pulmonary functions in children with segmental Mycoplasma pneumoniae pneumonia.
Liang-Xia WU ; Min WU ; Dan-Ping GU
Chinese Journal of Contemporary Pediatrics 2011;13(3):185-187
OBJECTIVETo investigate the changes of pulmonary functions in children with segmental Mycoplasma pneumoniae pneumonia (SMPP).
METHODSA total of 55 children with SMPP were recruited into this study. Pulmonary functions were measured at both acute and recovery phases, including FVC, FEV1, FEV1/FVC, PEF, FEF25%, FEF50%, FEF75% and FEF25%-75%.
RESULTSFVC, FEV1, FEV1 /FVC, PEF, FEF25%, FEF50%, FEF75%, and FEF25%-75% were reduced in all of the 55 cases at the acute phase. FEF25%, FEF50%, FEF75% and FEF25%-75% decreased more significantly. The indexes above mentioned were improved significantly at the recovery phase compared with the acute phase (P<0.05). During the acute phase FVC and FEV1 decreased more significantly in the group with multiple area lesions than in the group with single area lesions (P<0.05).
CONCLUSIONSBoth large and small airway functions are damaged in different degrees in children with SMPP during the acute phase. More cases show restrictive ventilatory disorders and the injury of small airway function is more severe. The pulmonary function is markedly improved at the recovery phase, suggesting that the pulmonary function impairments are reversible. The pulmonary function impairments are more severe in children with multiple area lesions.
Adolescent ; Child ; Child, Preschool ; Female ; Forced Expiratory Flow Rates ; Forced Expiratory Volume ; Humans ; Lung ; physiopathology ; Male ; Pneumonia, Mycoplasma ; physiopathology
10.Application of MiniFiler kit in forensic DNA testing of low copy number template.
Jian-ping TANG ; Dan WU ; Chen ZHANG ; Huai-gu ZHOU
Journal of Forensic Medicine 2007;23(4):304-306
OBJECTIVE:
To detect low copy number of DNA samples by using a newly launched commercial miniSTR detection kit (MiniFiler) in forensic practice.
METHODS:
Low concentration and/or challenged forensic DNA samples were analyzed according to protocols provided by the manufacturer (Applied Biosystems, Foster City, USA).
RESULTS:
DNA samples as low as 10 pg could be amplified by MiniFiler kit, and the optimal DNA quantity was 40 pg or above.
CONCLUSION
MiniFiler kit can be used for analysis of low copy number STR.
Alleles
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DNA Fingerprinting/methods*
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Forensic Genetics/methods*
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Genotype
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Humans
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Polymerase Chain Reaction/methods*
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Reagent Kits, Diagnostic
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Sensitivity and Specificity
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Tandem Repeat Sequences
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Templates, Genetic