Objective:To correlate N-terminal pro-B-type natriuretic peptide (NT-proBNP) and heart type fatty acid binding protein (H-FABP) levels with the prognosis of patients with heart failure.Methods:Forty-nine patients with severe heart failure who received treatment in Tongde Hospital from December 2017 to June 2019 were included in the observation group. Forty-nine healthy subjects who concurrently received health examination were included in the control group. The heart function of the 49 patients was graded by the New York Heat Association (NYHA) classification. The levels of NT-proBNP and H-FABP were measured in different NYHA grades of patients and the healthy controls. The value of NT-proBNP and H-FABP levels in the diagnosis of heart failure was investigated and NT-proBNP and H-FABP levels were correlated with prognosis of heart failure.Results:In the observation group, NT-proBNP and H-FABP levels were (341.52 ± 12.74) pg/mL and (69.01 ± 11.45) ng/mL, respectively, which were significantly higher than (189.60 ± 12.73) pg/mL and (37.25 ± 11.44) ng/mL, respectively, in the control group ( t = 59.047 and 13.735, both P < 0.001). In patients with NYHA III grade severe heart failure, NT-proBNP and H-FABP levels were (295.62 ± 42.38) pg/mL and (75.09 ± 19.15) ng/mL, respectively, which were significantly higher than (120.43 ± 21.69) pg/mL and (21.76 ± 5.22) ng/mL, respectively in patients with NYHA I grade severe heart failure, (203.51 ± 42.77) pg/mL and (44.92 ± 10.85) ng/mL in patients with NYHA Ⅱ grade severe heart failure ( F = 55.100, 46.040, both P < 0.001). NT-proBNP and H-FABP levels in patients with NYHA I grade severe heart failure were significantly lower than those in patients with NYHA Ⅱ grade severe heart failure ( t = 7.231, 8.130, both P < 0.001). The sensitivity and accuracy of detecting NT-proBNP and H-FABP levels alone in the diagnosis of heart failure (76.92%, 78.85%, 84.69%, 86.73%) were significantly lower than those of detecting NT-proBNP and H-FABP levels in combination (98.08%, 97.96%, χ2 = 75.760, 10.913, both P < 0.001). NT-proBNP and H-FABP levels were positively correlated with the prognosis of heart failure ( r = 0.362, 0.611, both P < 0.05). Conclusion:NT-proBNP and H-FABP levels are positively correlated with the prognosis of severe heart failure in older adult patients. Detecting NT-proBNP and H-FABP levels in combination can increase the sensitivity and accuracy in the diagnosis of severe heart failure.

Objective To evaluate the validity and responsiveness of the Chinese version of the miniBalance Evaluation Systems Test(mini-BESTest).Methods A total of 208 stroke patients were evaluated by 2 professionals using both mini-BESTest and Berg balance scale (BBS).All data were analyzed using Pearson correlation,t-test and descriptive statistical analysis.Results Significant correlation was observed between each entry of the mini-BESTest and its domain,with correlation coefficients ranging from 0.78 to 0.93 (P ＜ 0.05).The scores of whole assessment and the three related domains were significantly correlated between mini-BESTest and BBS results,the correlation coefficients ranging from 0.68 to 0.87 (P ＜ 0.05).After 2 weeks of treatment,there was significant improvement in each domain and in the total assessment score (P ＜ 0.05).No floor or ceiling effects were observed in the total mini-BESTest scores,but there was a slight ceiling effect in the anticipatory and sensory orientation domains,and a slight floor effect in the reactive postural control domain.Conclusion The mini-BESTest has good validity and responsiveness,and can be applied to evaluate patients after stroke.

Objective To research the effects of somatic sense interactive game Kinect on balance and walking function in stroke patients.Methods 40 patients with stroke were divided into control group (n=20) and experimental group (n=20), who accepted conventional rehabilitation and additional somatic sense interactive game Kinect. They were evaluated with gait analysis, Berg Balance Scale, Holden Walking Function Rating Scale and Barthel Index before and after treatment. Results Both groups improved in all the indices after 4 weeks of treatment (P<0.001), and improved more in the experimental group than in the control group (P<0.05). Conclusion The somatic sense interactive game Kinect could further improve the balance and walking function of stroke patients.

目的探讨以问题为基础的教学法(PBL)和康复病例实验报告两种教学法在运动疗法(PT)模块中的应用效果。方法选择2008 级四年制康复治疗学专业本科学生88 名，1 班采用PBL教学模式，2 班采用理论、实践相结合的康复病例采集与书写模式，采用自制教学效果评价表、学生实习跟踪调查进行评价。结果两班学生综合素质和分析问题的能力均有提高。2 班在观察能力、动手能力、理论与实践结合、疾病信息采集、分析判断能力、临床适应能力、服务态度与同情心、康复评定的全面性等方面好于1 班(P<0.05)，1 班在信息技术利用方面优于2 班(P<0.05)。结论融基础知识、专业知识、临床实践于一体，以书写康复病例为学科综合，培养学生以功能障碍为纲的临床思维，可以提高学生的动手能力、分析判断能力、医患沟通能力和临床适应能力，做到了课堂-见习-实习密切连接。

Objective To study the property of the K+ channels in mouse bone marrow stromal cells. Method Single-channel recording of the K+ channel currents in primary cultured mouse bone marrow stromal cell (BMSC) was performed using patch-clamp technique. Result A K+ channel with conductance of 16.7±1.4 pS was identified and characterized. The channel currents showed no inactivation in 75 s. Within a certain voltage range, the open time constant (to2) and open probability increased with depolarization, while the closed time constant (tc2) decreased. Conclusion The voltage-dependent K+ channel resembling the delayed rectifier K+ channel in excitable cells may play a role in the signal transduction of BMSC.

Objective To study the property of the K+ channels in mouse bone marrow stromal cells. Method Single-channel recording of the K+ channel currents in primary cultured mouse bone marrow stromal cell (BMSC) was performed using patch-clamp technique. Result A K+ channel with conductance of 16.7±1.4 pS was identified and characterized. The channel currents showed no inactivation in 75 s. Within a certain voltage range, the open time constant (to2) and open probability increased with depolarization, while the closed time constant (tc2) decreased. Conclusion The voltage-dependent K+ channel resembling the delayed rectifier K+ channel in excitable cells may play a role in the signal transduction of BMSC.

Objective To explore the interaction between hepatitis B virus(HBV) X protein and cytochrome C oxidase Ⅲ (COXⅢ).Methods Mating experiment and in vitro binding assays were utilized tO verify the interaction between HBV X protein and COX Ⅲ in vivo and in vitro respectively in yeast cells.Coimmunoprecipitation was employed to demonstrated the binding between these tWOproteins in mammalian cells.Results In mating experiment,the resulting diploids containing both pAS2-1-X and pACT2-COX Ⅲ plasmids turned blue in the β-gal assay,the result of Western blot displayed a positive reactive strap which indicated X protein could interact directly with COX Ⅲ in vitro binding assays.Interaction between these two proteins in mammalian cells was further confirmed by coimmunoprecipitation,as a protein strap about 17 000 was detected in Western blot.Conclusions It is speculated the specific interaction may exist between HBV X protein and COX Ⅲ in both prokaryotie and mammalian cells.X protein may impair the mitochondrial respiration chain and energy metabolism through its association with COX Ⅲ.

OBJECTIVETo investigate the risk factors and preventative measures for neonatal pneumothorax.

METHODSRetrospective analysis was performed on the clinical data of 2286 neonates who were hospitalized in the neonatal intensive care unit between October 2010 and November 2011, and a case-control study was conducted to analyze the risk factors and preventative measures for neonatal pneumothorax.

RESULTSThe incidence of pneumothorax among the neonates was 1.57% (36/2286), and it was significantly higher in full-term infants than in preterm infants (23/1033 vs 13/1253, P=0.023). Logistic regression analysis indicated that cesarean section, neonatal respiratory distress syndrome (NRDS), wet lung, pneumonia and mechanical ventilation were the independent risk factors for neonatal pneumothorax (odds ratios=7.951, 6.090, 7.898, 6.272 and 4.389; P<0.05 for all). The higher the peak inspiratory pressure (PIP) during mechanical ventilation, the higher the incidence of neonatal pneumothorax (P<0.001). Pulmonary surfactant reduced the incidence of pneumothorax among neonates with NRDS (2.9% vs 10.1%; P=0.006).

CONCLUSIONSNeonatal pneumothorax occurs mostly in full-term infants. Cesarean section, NRDS, wet lung, pneumonia and mechanical ventilation are closely associated with neonatal pneumothorax. Strict management of indications for cesarean section, keeping PIP at a low level during mechanical ventilation, and use of pulmonary surfactant are helpful in preventing neonatal pneumothorax.

Case-Control Studies ; Cesarean Section ; adverse effects ; Female ; Humans ; Infant, Newborn ; Logistic Models ; Male ; Pneumothorax ; etiology ; prevention & control ; therapy ; Respiration, Artificial ; adverse effects ; Respiratory Distress Syndrome, Newborn ; complications ; Retrospective Studies

Structural modifications were performed with natural product of oleanolic acid to search for novel anticancer drugs. Ten oleanolic acid derivatives were designed and obtained by the reaction of oxidation, acylation or hydrolyzation, etc. The cytotoxic activity of derivatives was evaluated against HeLa, HepG2 and BGC-823 cells in vitro by MTT assay, gefitinib and etoposide used as a positive control. The results showed that compound 5a was particularly active to inhibit HepG2 cells growth, and anti-tumor activity of compound 7 on HeLa cells was significantly stronger than oleanolic acid. They are worthy to be studied further.

AIM: To investigate the biocolonization of poly 2-hydroxyethy methacrylate (PHEMA) sponge with cornea tissue and evaluate the therapeutic effects of modified porous poly 2-hydroxyethy methacrylate-Polymethyl met-hacrylate (PHEMA-PMMA) Keratoprostheses (KPro) on rabbit and monkey corneas. METHODS:The KPro were made using two-stage polymerization combined with mechanical cutting. The experiment was divided into two groups. In the control group, ten normal rabbit eyes received lamellar implanta-tion of PHEMA sponges. The sponges were obtained 2 weeks, 1,2,3 and 4 months after operation. The cell proliferation and neovascularization inside the sponges were observed using light and transmission electron microscopy (TEM) and immunohistochemistry. In the experimental group, the porous PHEMA-PMMA KPros were inserted into the lamellar pockets of eight rabbit corneas and two monkey corneas (stage I operation). The healing process was investigated by slit-lamp microscopy. The anterior lamellar cornea tissues were removed 3 months after surgery, exposing the under-neath transparent core (stage II operation). The operated eyes were then followed up for 3-6 months.light microscope, fibroblasts started to grow into the cornea 2 weeks after operation; lots of cells, accompanied with new blood vessels, invaded into the cornea 2-3 months after surgery. Invading cells of sponge, as well as keratocytes, were positive for vimentin. Under the electron microscope, the invading cells looked healthy and were surrounded by extracellular matrix and collagen. In 8 rabbit eyes which received KPro implantation, anterior lamellar cornea melting happened in two eyes after the stage II operation. The remaining 6 corneas retained their central cores during observation after the stage II operation.Two monkey operated eyes were found no complication thoughout the whole follow-up.cornea. The modified PHEMA-PMMA KPros have obtained a relatively stable results after implantation into animal corneas.