1.Application of antibody cocktail method in the immunohistochemistry.
Hong YANG ; Ke LI ; Dan-dan DONG
Chinese Journal of Pathology 2005;34(3):182-183
Actins
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immunology
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metabolism
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Antibodies, Monoclonal
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analysis
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Antigens, CD34
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immunology
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metabolism
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Breast Neoplasms
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metabolism
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Female
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Humans
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Immunohistochemistry
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methods
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Lung Neoplasms
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metabolism
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Membrane Proteins
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immunology
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metabolism
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Tumor Suppressor Protein p53
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immunology
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metabolism
2.Effect of Preoperative Chemotherapy on Malignant Extragonadal Germinoma in Children
wei, YANG ; huan-min, WANG ; hong, QIN ; xiu-dan, ZHU
Journal of Applied Clinical Pediatrics 2006;0(22):-
Objective To explore the effect of preoperative chemotherapy for children with malignant extragonadial germinoma. Methods Twenty patients with malignant extrogonadal germinoma had been treated with preoperative chemotherapy from Mar.2003 to Mar.2007.Protocol 1 of chemotherapy was to improve PEB:(cisplatin plus etoposide plus bleomycin A5);Protocol 2 was VAC(vincristine plus actinomycin D plus cyclophosphamide) the average time span of the chemotherapy was 16 weeks.Upon completion of chemotherapy,tumor resection was performed.The content of alpha fetoprotein(AFP) was determined before operation and compared with the content before chemotherapy.Re-gular follow-up procedure was taken after operation. Results All 20 patients received chemotherapy and underwent operation.The removal rate of resection operation was 100%,the process of operation was smooth.The volume of tumors in 14 patients decreased over 50% compared with those before chemotherapy.The response rate was 70%.The content of AFP decreased significantly in 15 patients(75%).Fifteen patients had been living without tumor for up to 2 years,for 5 cases the survival time went beyond 5 years. Conclusions Malignant extrogonadal ger-minoma in children grow infiltratively,bring detrimental effects to neighboring organs,and bring obstacles to resection operation.Chemotherapy for children with malignant extragonadal germinoma before operation can reduce volume of tumor and bleeding during operation,raise the resectability rate and lower the content of AFP.It will enhance the effectiveness of operation and improve prognosis.Therefore,it can be used as a conventional clinical method.
3.Efficacy of blood purification on acute trichloropropane poisoning.
Hong-Jun YANG ; Zhi CHEN ; Li-Dan GAO
Chinese Journal of Industrial Hygiene and Occupational Diseases 2010;28(1):56-57
Adult
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Female
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Hemofiltration
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Humans
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Hydrocarbons, Chlorinated
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poisoning
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Male
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Middle Aged
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Poisoning
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therapy
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Treatment Outcome
4.Observation on Therapeutic Effect of Acupucnture plus Rehabilitation for Hemiplegia Following Stroke
Dan YANG ; Chuanen ZHANG ; Li XU ; Jue HONG
Journal of Acupuncture and Tuina Science 2008;6(4):219-221
Objective: To observe the therapeutic effect of acupuncture plus rehabilitation on hemiplegia following stroke. Methods: Ninety-six patients with hemiplegia due to stroke were randomly allocated into two groups. The treatment group (50 cases) was treated with acupuncture plus rehabilitation, and the control group (46 cases) was treated with rehabilitation. Results: The total effective rate was 92.0% in the treatment group and 76.1% in the control group. There was a statistical difference in the effects between the two groups (P<0.05). Conclusion: Acupuncture plus rehabilitation is a perfect method for hemiplegia following stroke. It is especially effective in improving the function of limbs, relieving the spasm and improving the activity of daily life.
5.Analysis on composition regularity of carthami flos-containing traditional Chinese medicines.
Yu-Fei GUO ; Dan SHEN ; Hong-Jun YANG
China Journal of Chinese Materia Medica 2014;39(11):2144-2148
To analyze the composition regularity of Carthami Flos-containing prescriptions of the Drug Standards of Ministry of Health of People's Republic of China-Traditional Chinese Medicine Preparations (the ministerial standards for Traditional Chinese Medicine) based on the traditional Chinese medicine inheritance support system (TCMISS, RZDZ No. 0389952). Efforts were made to identify 331 prescriptions containing Carthami Flos and summarize 16 attending functions and 10 commonly used drug combinations. Three commonly used drug combinations were selected for an in-depth analysis on Carthami Flos's combined administration regularity. Based on Carthami Flos's attending functions, its effects in paralysis, traumatic injuries and dysmenorrheal were compared to analyze Carthami Flos's core drug combinations for treating different diseases. The regularity of clinical administration and the characteristics of commonly used drug combinations were summarized to provide reference for Carthami Flos's clinical application and new ideas for new drug R&D. Carthami Flos prescriptions was mainly used to treat blood stasis and pain and mostly combined with drugs that could activate blood, promote the circulation of qi and dispel pathogenic wind to treat Qi-stagnation and blood stasis caused by various pathogenic factors such as wind, cold and dampness.
Carthamus
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chemistry
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Clinical Trials as Topic
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Drug Prescriptions
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Drug Therapy
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Drugs, Chinese Herbal
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chemistry
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therapeutic use
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Flowers
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chemistry
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Humans
6.Analysis of characteristics of using traditional Chinese and western medicine on type 1 diabetes adult patients based on electronic medical records.
Hong-Hong ZHANG ; Yan-Ming XIE ; Wei YANG ; Yong-Yan WANG ; Dan-Hui YI ; Yan ZHUANG
China Journal of Chinese Materia Medica 2014;39(18):3504-3508
To understand the kinds and the characteristics of combination of traditional Chinese medicines and western medicines using on adults with type 1 diabetes in general hospitals,This research brings into 4 602 hospitalized adult patients with type 1 diabetes from hospital information system (HIS) of 13 third class A hospitals. These research objects were hospitalized in December 2003-July 2011. The research analyzes the operating frequency and associated usage of western medicines and traditional Chinese medicines by the method of frequency statistics and association rules. Through the analysis, the research says that in the clinical treatment of adult patients with type 1 diabetes, the western medicine used most frequently is insulin, a total of 1 539 cases, accounted for 8.47%; the traditional Chinese medicine used most frequently is oral agents of pseudo-ginseng, a total of 183 cases, accounting for 6.25%; the combinations of Chinese and western medicines commonly used include Huoxue Huayu Tongmai Shuluo decoction + vasodilator, support degree is 45.93%, followed by Huoxue Huayu Tongmai Shuluo decoction + vasodilator + hypoglycemic drugs, support degree is 45.50%; Huoxue Huayu Tongmai Shuluo decoction + vasodilator + nutritional agent, support degree is 36.29%.
Adult
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Aged
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Aged, 80 and over
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Diabetes Mellitus, Type 1
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drug therapy
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Drugs, Chinese Herbal
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therapeutic use
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Electronic Health Records
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Female
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Humans
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Hypoglycemic Agents
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therapeutic use
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Insulin
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therapeutic use
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Male
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Medicine, Chinese Traditional
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methods
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Middle Aged
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Panax
7.Effect of PI3K Inhibitor on Proliferation of Human Glomerular Mesangial Cells
Li MA ; Chunjiang ZHANG ; Hong LI ; Dan ZHAO ; Jianping CHEN ; Jun YANG ; Xiaoping YANG
Tianjin Medical Journal 2014;(10):969-971,1059
Objective To investigate the effect of LY294002 on proliferation of cultured human glomerular mesan-gial cells, and to study on the mechanism underlying this proliferation. Methods Different concentrations of LY294002 (0.02,0.2,2,20,100 mg/L) were administrated to the cultured human glomerular mesangial cells. The effects of mesangial cell proliferation were measured using CCK-8 colorimetric assay. Under appropriate concentrations, proliferation of cultured mesangial cells were measured using CCK-8 at 0.5, 1, 24, 48 hours of drug administration time. Results LY294002(0.02 mg/L)didn’t obviously inhibited the growth of mesangial cells(P>0.05), the inhibition rate was 5.05%. The effect of LY294002 on the cells decreased with rising concentration (0.2 to 20 mg/L) in a dose-dependent manner (P<0.05). Mesan-gial cells would stop growing with the increasing concentration. LY294002 didn’t obviously inhibited the growth of mesan-gial cells at 2 mg/L during 0 to 1 h(P>0.05), but inhibited proliferation gradually from 1 to 48 h (P<0.05). Conclu-sion Within the certain range of concentration and time, the LY294002 inhibits the proliferation of human glomerular me-sangial cells, and PI3K/Akt/mTOR signaling pathway may regulate the proliferation of human mesangial cell.
8.Effects of mesenchymal stem cells on pathological changes and cytokines expression in rabbit model of autoimmune dry eye
Xilian WANG ; Xiaoxiao LU ; Liyuan YANG ; Hong NIAN ; Dan YANG ; Ruihua WEI
Recent Advances in Ophthalmology 2017;37(8):714-718
Objective To establish the model of autoimmune dry eye and analyze the level of cytokines and the pathological changes of autoimmune dry eye rabbits treated with human umbilical cord mesenchymal stem cells (MSCs).Methods Twentyfour female New Zealand rabbits were randomly divided into three groups:Normal control group,dry eye group and MSCs treatment group,8 cases in each group.After treated with MSCs for six weeks,the pathological changes were observed,and the expression of Th1 and Th17 cell differentiation related cytokines mRNA in lacrimal gland tissue was detected by real-time quantitative PCR.The percentage of regulatory T cells (CD4 +Foxp3 + cells) in the lacrimal gland and spleen tissues of the dry eye model group and MSCs treatment group was measured by flow cytometry.Results After treatment with MSCs for 6 weeks,HE staining from each rabbit lacrimal gland showed that,the normal control group showed no or only a few lymphocytes;The lacrimal gland cells were atrophied in the dry eye group,the distribution of lymphocyte scattered around the gland and small blood vessels;Compared with dry eye group,the lacrimal gland lymphocyte infiltration reduced in MSCs treatment group,and the cell morphology were better.Compared with dry eye group,lymphocyte infiltration and aggregation in the conjunctival conjunctiva in MSCs treatment group were significantly reduced,the epithelial structure was intact,and the degeneration and atrophy cells were rare.The expression of inflammatory factors including interferon-γ(IFN-γ),Interleukin-17 (IL-17) and transcription factor T-bet mRNA were decreased in MSCs treatment group,there were significant difference compared with dry eye group (all P < 0.05);Thl7 cell associated cytokines IL-17 expression levels were decreased,but no significant difference was found between two groups (P > 0.05);The expression of transcription factor RORrt mRNA in dry eye group was significantly decreased,the difference was statistically significant (P < 0.05).The expression of inflammatory factors TNF-α in the lacrimal gland tissue of MSCs treatment group was significantly lower than that in the dry eye model group,while the expression of the anti-inflammatory factor TGF-β was significantly higher than that in the dry eye model group,and the differences were statistically significant (all P<0.05).The regulatory T cells (CD4 + Foxp3 + cells) in the lacrimal gland of dry eye model group accounted for 10% of lymphocytes was,while the treatment group accounted for 27.8%,MSCs group was significantly higher than dry eye group,the difference was statistically significant (P < 0.05).Conclusion MSCs can reduce the histopathological changes of the immune dry eye,and they may have immunoregulatory effects on autoimmune dry eye.The mechanisms of immunomodulatory effects may be related to the balance of inflammatory cytokines and anti-inflammatory cytokines.
9.Clinical and pathological analysis of mesenchymal hamartoma of liver.
Dan LI ; Xiang-Hong YANG ; Xiao-Yin CHANG
Chinese Journal of Contemporary Pediatrics 2011;13(8):684-685
Child
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Child, Preschool
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Female
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Hamartoma
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diagnosis
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etiology
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pathology
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Humans
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Immunohistochemistry
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Infant
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Liver Diseases
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diagnosis
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etiology
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pathology
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Male
10.Papillary glioneuronal tumor: report of a case.
Ji-ping QI ; Hong ZHU ; Dan-yang LI ; Huan-lin MEI
Chinese Journal of Pathology 2006;35(12):764-765
Adolescent
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Brain Neoplasms
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metabolism
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pathology
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surgery
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Diagnosis, Differential
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Ependymoma
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metabolism
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pathology
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Ganglioglioma
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metabolism
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pathology
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surgery
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Glial Fibrillary Acidic Protein
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metabolism
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Humans
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Immunohistochemistry
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Male
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Tubulin
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metabolism
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Vimentin
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metabolism