AlM:To study and observe the change of retrobulbar artery hemodynamics and bulbar conjunctival microcirculation indexes of patients with optic atrophy.METHODS: Seventy patients with optic atrophy in our hospital from April 2013 to October 2014 were selected as observation group, 70 healthy persons with the same age at the same time were the control group. Retrobulbar artery hemodynamics and bulbar conjunctival microcirculation indexes of two groups were compared, those detection indexes of observation group with different types and severity degree were compared too.RESULTS: The retrobulbar artery hemodynamics and bulbar conjunctival microcirculation indexes of observation group were all worse than those of control group, and the detection results of observation group with severe optic atrophy were worse than those of patients with mild and moderate optic atrophy, and the detection results of patients with moderate optic atrophy were all worse than those of patients with mild optic atrophy ( all P < 0. 05 ), there were all significant differences, while the detection results of patients with different types of optic atrophy had no significant differences (all P>0. 05). CONCLUSlON: The change of retrobulbar artery hemodynamics and bulbar conjunctival microcirculation indexes of patients with optic atrophy are great, and the influence of the disease severity degree for the detection results are great.

The aim of laboratory evaluation of basic education is to improve the quality of experimental teaching,which demands thorough reforms of many aspects,including the ranks of teachers,administration,content,methods and instrument of teaching. This article summarizes the experience and achievement of Functional Laboratory Centre,Central South University during under- graduate teaching evaluation by the Ministry of Education in 2006.

Objective Analyse the change of left ventricular (LV) longitudinal and radial strain in patients with aortic regurgitation (AR) and discuss the relationship between the 2D strain parameter and the filling and ejection of LV. Methods Thirty healthy controls and 45 patients with AR (24 patients with moderate AR and 21 with severe AR) were enrolled in this study, LV systolic global peak radial strain(GRS), systolic global peak longitudinal strain(GLS) and systolic peak longitudinal strain(S), systolic peak longitudinal strain rate(SRs), early diastolic peak longitudinal strain rate(SRe) of every segment were measured or calculated using 2D-STE, early and late diastolic transmitral flow velocity (E, A) were recorded by pulsed Doppler echocardiography and early diastolic mitral annular velocity (Ea) were assessed by tissue Doppler imaging,the E/A and E/Ea ratio were calculated. Discuss the relationship of GLS and LV ejection fraction (LVEF), GLS and E/Ea using the Pearson correlation analysis. Results The GLS were (-20.09±1.47)%, (-18.68±1.52)%, (-12.56±3.25)%and the GRS were (46.71±7.65)%, (43.01±5.95)%, (28.52±6.13)% in control group, patients with moderate and severe AR (MAR group and SAR group) respectively. There were significant differences among the groups (F =82.08,47.69, both P < 0.01) as following:SAR group with control group and MAR group [ q=17.56,13.60 (GLS), q=13.44, 10.20 (GRS), all P<0.01),MAR group and control group [ q=3.42 (GLS), P<0.01]. The SRs of the apical segment were (-1.24±0.22)s-1, (-1.19±0.25)s-1, (-1.04±0.28)s-1 in control group,MAR group and SAR group respectively. There were significant differences among the groups (F=4.47, P < 0.05) as following:SAR group with control group and MAR group ( q=4.02,3.28, both P<0.01). The S, SRe of apical segment and the S,SRs,SRe of basal and midventricular in MAR group were all lower than the control group ( q=4.42, 5.01, 3.48, 3.24, 4.78, 4.12, 3.61, 6.72, all P < 0.01). Pearson correlation analysis revealed the GLS had a relationship with LVEF and E/Ea ( r=-0.73, 0.64, both P<0.01). Conclusion The reduced longitudinal strain and strain rate could detect LV dysfunction in patients with AR in early stage and the GLS had the ability to reflect the diastolic filling and systolic ejecting of the LV.

AIM:To investigate the role of potassium channel expression alteration in chronic cigarette smoking-induced increase in pulmonary vascular responsiveness,the effect of chronic cigarette smoking on large-conductance calcium-activated potassium channel(BKCa) and voltage-dependent delayed rectifier potassium channel(Kv1.5) expression in rat pulmonary smooth muscle cells were investigated in vivo.METHODS: HE staining,immuno-histochemistry and in situ hybridization techniques were used.RESULTS:(1) Chronic cigarette smoking downregulates the protein and mRNA expression of BKCa in pulmonary arterial smooth muscles.(2) Chronic cigarette smoking downregulated the protein and mRNA expression of Kv1.5 in pulmonary arterial smooth muscles.(3) In big artery,BKCa decreased more makedly than Kv1.5,but in small artery,both of them decreased equally.CONCLUSION: Chronic cigarette smoking downregulates the levels of BKCa and Kv1.5 in rat pulmonary arterial smooth muscle cells in vivo,which maybe contribute to the mechanism of cigarette smoking-induced increase in pulmonary vascular responsiveness.

Objective To investigate the effect of smoking and smoking cessation on the phosphorylation of IKK-β in type 2 diabetic rats. Methods Forty-two six-week-old Wistar rats were randomly divided into 4 groups: normal control(NC, n =7), diabetes control (DC, n =7), diabetes with smoking (DS, n = 14) and diabetes with smoking cessation(SC, n = 14). Rats in DS and SC groups were further assigned randomly into 8w and 12w subgroups. DS group was given passive smoking twice a day for 8 or 12 weeks, while SC group ceased passive smoking for 4 weeks after 8 or 12 weeks of smoking . Western blot method was used to detect the level of IKK-13 phosphorylation in skeletal muscle. Results Compared with the NC group,the phosphorylation of IKK-β protein in DC group was increased (0. 16±0. 05 vs 0. 30±0. 08, P < 0. 01). There was an increasing trend with the phosphorylation level of IKK-β in the DS (8w) subgroup, but there was no statistical difference between the DC group and SC(8w) subgroup (0. 40±0. 09 vs 0. 30±0. 08,0. 36±0. 10, P >0. 05). The phosphorylation level of IKK-β in DS(12w) group increased obviously, being significantly higher than that in the DC group and SC (12w) subgroup(0. 74 ± 0. 11 vs 0.30±0.08,0.35±0.07,P < 0.01). Conclusion With the prolongation of smoking duration, the phosphorylation of IKK-β in type 2 diabetic rats increased. After smoking cessation, the phosphorylation of IKK-β decreased. The phosphorylation of IKK-β may be involved in the mechanism by which smoking causes type 2 diabetes.

We have developed an HPLC method for the determination of tirapazaming (3-Amino-1,2,4-Benzotriazine -1,4-dioxide, TPZ) in rabbits and have studied the related pharmacokinetics in rabbits. After protein was precipitated, TPZ in plasma was determinated on HPLC with UV detection at 266 nm, and the mobile phase consisted of methyl alcohol and phosphoric buffer (10: 90, adjusted to pH6.5 +/- 0.1 with NaOH). TPZ and endogenous substances could be separated. Its calibration curve was linear (r=0.9995) within the range of 0.150-59. 98 mg/L, the detection limits was 0.5 ng (S/N>3), the recovery was more than 95% (n=3), the intra-day and inter-day precision was less than 6.0% (n = 5). The concentration-time curve of TPZ in rabbit plasma could be fitted to two-compartment model. These data indicate the method is sensitive, simple and reliabe. It is suitable for the pharmacokinetic study of TPZ in rabbits.
Animals ; Chromatography, High Pressure Liquid ; Rabbits ; Radiation-Sensitizing Agents ; pharmacokinetics ; Sensitivity and Specificity ; Triazines ; blood ; pharmacokinetics

Objective To evaluate the effect of ice-made compound drug liquid on serious oral ulcer caused by chemotherapy.Methods Selecting sixty cases of Ⅲ～Ⅳ degree oral ulcer caused by chemotherapy in our department,and randomly dividing them into a control group and an experimental group.In the control patients were offered an ice-made compound drug liquid to cue the oral ulcer.The effect of each of the therapy was divided into three degrees, i.e., ineffective, effective and excellent, and the effects of the two different ways were compared.Results In the control group, the number of patients that got ineffective, effective and excellent results was 4, 18 and 8, separately; and in experimental group, the number of patients that got ineffective, effective and excellent result was 1, 3 and 26, separately.The difference on the effect between the two groups was significant (u = 4.476,P＜0.01 ).Conclusions The therapeutic effect of ice-made compound compound drug liquid is easy to be made and rapidly come into effect.It is worthy of recommending widely.

Adult ; Antiviral Agents ; pharmacology ; therapeutic use ; DNA, Viral ; Female ; Genotype ; Hepatitis B virus ; genetics ; Hepatitis B, Chronic ; drug therapy ; virology ; Humans ; Lamivudine ; pharmacology ; therapeutic use ; Male ; Middle Aged ; Treatment Outcome ; Young Adult

Objective To investigate the immediate therapeutic effect of medicated thread moxibustion on cervical spondylosis.Methods Seventy patients with cervical spondylosis meeting the inclusion criteria were randomly allocated to treatment and control groups by using random number table method, 35 cases each. The treatment group received medicated thread moxibustion and the control group, percutaneous superficial needling. The Visual Analogue Scale (VAS) score and the Clinical Assessment Scale for Cervical Spondylosis (CASCS) score were recorded in the two groups before treatment and at 30 min and 24 hrs after treatment. The clinical therapeutic effects were compared between the two groups. Results The VAS and the CASCS scores decreased significantly in the two groups at 30 min and 24 hrs after treatment compared with before treatment (P<0.05,P<0.01). There was no significantly statistical difference in comparing the VAS score between the two groups after the treatment (P>0.05). The CASCS score was lower in the treatment group than in the control group at 30 min after treatment (P<0.01) and lower in the control group than in the treatment group at 24 hrs after treatment (P<0.05). At 30 min after treatment, the clinical therapeutic effect in the treatment group was superior to that in the control group (P<0.01); at 24 hrs after the treatment, the clinical therapeutic effect in the control group was superior to that in the treatment group (P<0.05).Conclusions Medicated thread moxibustion has a definite immediate therapeutic effect on cervical spondylosis.

Recombinant human interleukin-1 receptor antagonist was expressed in E. coli as an insoluble inclusion body. The inclusion body was dissolved in the 8 M urea and then the solution was diluted untill the concentration of urea became 2 M. By ion exchange chromatography the protein in the solution of 2 M urea was refolded and purified. At last the purity of product is more than 95% and its bioactivity is more than 1 x 10(5) IU/mg while it has little endotoxin. Western-Blotting also indicates that recombinant protein can react with antibodies against anti-hIL-1ra.
Escherichia coli ; genetics ; metabolism ; Humans ; Inclusion Bodies ; metabolism ; Interleukin 1 Receptor Antagonist Protein ; biosynthesis ; genetics ; Protein Folding ; Recombinant Proteins ; biosynthesis ; genetics ; isolation & purification