Objective To investigate IL-1?mRNA expression and protein secretion after ultraviolet irradiation in human keratinocyte(KC)originated SCC12F cells.Methods Expression of IL-1?mRNA was detected by Northern blot and the secretion level of IL-1?protein was analyzed by ELISA.Results There was a spontaneous expression of IL-1?mRNA in SCC12F cells in culture system of normal KC,which was increased along with the culture time,and reached the highest expression level in120hours.The secretion level of IL-1?protein was increased in a time-and dose-dependent manner in SCC12F cells after ultraviolet B(UVB)irradiation.Conclusions SCC12F cells may express IL-1?mRNA spontaneously under regular KC media.The secretion level of IL-1?protein shows both time-and dose-effect after UVB irradiation.

Disease Notification ; Humans ; Internet ; Occupational Diseases

Bronchial Arteries ; diagnostic imaging ; Embolization, Therapeutic ; methods ; Hemoptysis ; therapy ; Humans ; Male ; Middle Aged ; Myocardial Infarction ; etiology ; Radiography

Objective To investigate the relation between the expression of HSP70 and p57kip2 in C, CT and Enneking surgical classifi-cation by detecting expression of HSP70 and p57kip2. Methods 30 cases of GCT were collected, including surgical operation specimens and 13 cases of osteochondroma were taken as control group. The expression of HSP70 and p57kip2 were detected, and the relation between the ex-pression of HSP70 and p57kip2 and Enneking surgical classification and prognosis of GCT were analyzed. Results With the increase of tumor Enneking surgical grades, the positive rate of HSP70 was increased and the negative rate of p57kip2 was decreased. Expression rates of HSP70 were 22. 2%, 88.9% ,100% in grades of 1～3 and expression rates of p57kip2 were 88. 9%, 88.9%, 33.3% in grades of 1～3. There was a negative correlation between HSPTo and p.57kip2 expression. Conclusions HSP70 and p57kip2 may play a role in the genesis, and develop-ment of giant cell tumor of bone, and it can be helpful for surgical classification and prognesis of GCT.

The incidence of invasive fungal sinusitis is low, on the rising in recent years. But the misdiagnosis rate of the disease is high. In order to improve the level of early diagnosis and reduce the misdiagnosis, in this pa per, the invasive fungal sinusitis's clinical characteristics, diagnosis, treatment and prognosis are reviewed.
Humans ; Mycoses ; diagnosis ; therapy ; Sinusitis ; diagnosis ; therapy

A patient diagnosed with myoepithelial carcinoma was recently treated in our department. The neoplasm was huge, located in the left maxillofacial region, blocking both eyes, impeding feeding. About one month before admission, the tumor began to bleed frequently, about 100 ml each time, causing headache, dizziness, fatigue, and cold sweats. CT showed the maximum diameter of the tumor was about 23 cm, with uneven density, and maxillofacial bone destruction. MRI revealed internal bleeding and necrosis inside the tumor. After admission, blood routine test showed erythrocyte count 3.64 x 10(12)/L(↓), hemoglobin 106 g/L(↓), hematocrit 0.320 (↓), serum iron 6.2 μmol/L(↓). After surgery, the patient recovered smoothly.
Carcinoma ; diagnosis ; surgery ; Humans ; Magnetic Resonance Imaging ; Myoepithelioma ; diagnosis ; surgery ; Nasal Cavity ; pathology ; Paranasal Sinus Neoplasms ; diagnosis ; surgery ; Paranasal Sinuses ; pathology

The incidence of oncocytic papilloma of nasal ethmoid sinus is very low. In order to improve the understanding of the disease for clinicians,we report one case of oncocytic papilloma in nasal ethmoid sinus. The case performs mainly congestion of the left nasal cavity, running pus nose and hyposmia. Nasal endoscopy found new creature in the meatus,and CT indicates soft tissue shadow in the left superior nasal passages,ethmoid sinus involved. Tumor is resected completely. There is no recurrence after five years follow-up.
Aged ; Humans ; Male ; Nasal Cavity ; Nose Neoplasms ; Papilloma ; Paranasal Sinus Neoplasms

Objective To evaluate the effect of hypothermia on the pharmacokinetics of rocuronium in the pigs with hemorrhagic shock.Methods Thirty-two Bama mini pigs of both sexes,aged 4-6 months,weighing 22-25 kg,were randomly divided into 4 groups (n=8 each) using a random number table:normal temperature control group (group NC),normal temperature+hemorrhagic shock group (group NH),hypothermia control group (group HC),and hypothermia+hemorrhagic shock group (group HH).NC and NH groups were put in the normal temperature environment,and HC and HH groups were put in a freezer at-15 ℃.In NC and HC groups,rocuronium 3.78 mg/kg was injected via the auricular vein after the animals were awake.In NH and HH groups,hemorrhagic shock was then induced by removing 40％ of blood volume from the right femoral artery at a constant speed within 15 min (30 ml/kg),and rocuronium 3.78 mg/kg was injected via the auricular vein at 30 min after the model was successfully established.At 0 (T0),2 (T1),4 (T2),7 (T3),10 (T4),15 (T5),20 (T6),30 (T7),60 (T8),120 (T9),180 (T10),240 (T11) and 300 min (T12) after rocuronium injection,blood samples were collected from the internal jugular vein for determination of the blood concentration of rocuronium by high performance liquid chromatography-tandem mass spectrometry method.The maximum concentration (Cmax),elimination half-life (t1/2),plasma effect-site equilibration rate content (Ke0),area under concentration curve,and mean residence time (MRT) of rocuronium were calculated.Results Compared with group NC,the blood concentration of rocuronium was significantly decreased at T5-T12 in group NH,the blood concentration of rocuronium was significantly increased at T5-T12 in group HC,and t1/2 was significantly prolonged,Ke0 was decreased,and MRT was prolonged in NH and HC groups (P＜0.05 or 0.01).Compared with group HC,the blood concentration of rocuronium was significantly increased at T4-T12,t1/2 was prolonged,Ke0 was decreased,and MRT was prolonged in group HH (P＜0.05).Compared with group NH,the blood concentration of rocuronium was significantly increased at T5-T11,t1/2 was prolonged,Ke0 was decreased,and MRT was prolonged in group HH (P＜ 0.05).Conclusion Hypothermia can reduce the pharmacokinetics of rocuronium in the pigs with hemorrhagic shock.

Formative assessment is in conformity with the teaching concept of problem-based learning (PBL).It is to make a real-time,dynamic and sustainable observation and evaluation in the whole teaching process.It is helpful to improve learning and teaching continuously.The PBL clinical teaching courses of long-schooling program in our school obtained the certain effect.It used the formative evaluation mode.The homemade students mutual table and teacher evaluation table were used in PBL course.Specifically,students' self-evaluation and mutual-evaluation were made,and then teacher gave some comments in 10 minutes before the end of each class.Every student was scored by the group leader and teacher according to all these abilities,such as the power of expression,question ability,communication skills,coordination skills and the ability to access.At the end of one case study students wrote self assessment reports,and then the teacher wrote reviews,graded and gave feedback to students.However,the final scores were archived as data instead of being part of the course grade.The application of formative assessment has promoted the teaching effect of PBL.However,in the following practice,teachers should be targeted to solve the problems such as lack of teachiers,the students' self evaluation and mutual evaluation being not fair and highly recognized,and a few students having slack psychology.

Objective:To investigate influence of living locations on the personality characteristics of the only child and none-only children in an air force academy.Methods:1921 cadets were tested by the Chinese version of the MBTI-G.Results:Scores of the only child cadets from cities on the Sensing-Intuition and Thinking-Feeling dimensions were higher than those of none-only children group from cities and those of the only child group from countries.The scores of them on the SN scale were 93.0?18.5,90.4?17.6 and 90.1?17.7(t=2.25,P=0.025;t=1.99,P= 0.048).The scores of them on the TF scale were 94.6?22.6,89.1?21.1 and 88.4?21.0(t=4.02,P