OBJECTIVE:To identify Descurainia sophia and Semen Plantaginis by high performance capillary electrophoresis (HPCE). METHODS: HPCE was conducted with a fused silica capillary tube(60 cm?75 ?m) taking 30 mmol? L-1 sodium dihydrogen phosphate as running buffer (pH 8.2). The sample injection was performed by high pressure for 5 s at a separation voltage of 12 kV, an UV detection wavelength of 330 nm. RESULTS: The differences of HPCE electrophoregrams between Descurainia sophia and Semen Plantaginis were significant and easily to be differentiated. CONCLUSION: This method is simple, rapid and sensitive, and it can be applied to identify Descurainia sophia and Semen Plantaginis.

OBJECTIVE: To establish an HPCE and polymerase chain reaction (PCR) assay for identification of Descurainia sophia and Plantago asiatica.METHODS: PCR products of D. sophia and P.asiatica were analyzed by capillary tube (60 cm?75 ?m), taking 30 mmol?L-1 sodium tetraborate decahydrate (pH 9.2) as run buffer. The injection sample was performed by high pressure of 5 s at a separation voltage of 12 kV and with UV detection wavelength of 254 nm. RESULTS: PCR products of D. sophia and P. asiatica were separated by HPCE rapidly and accurately. The significant differences were noted in electrophoregram between PCR products of D. sophia and P. asiatica.CONCLUSION: This method is simple, rapid and sensitive for identification of D.sophia and P. asiatica.

Objective To investigate the clinical features of unintentional injuries in children,and to provide the theoretical basis for preventing and intervening the occurrence. Methods We collected the data of children aged from 0 to 14 years old and hospitalized in the First Affiliated Hospital of Guangxi Medical University from January 2013 to December 2015. Results There were 2286 cases of children including 1507 boys(65. 92%) and 779 girls(34. 08%). The high incidence age was 1 to 3 years with a total of 896 cases(39. 19%). The first five causes of unintentional injuries were burns/scalds 840 cases(36. 71%),fall/drop 464 cases(20. 30%),blunt force injury 290 cases(12. 68%),foreign bodies(in the esophagus,airway, etc) 253 cases(11. 07%),and sharp object injury 153 cases(6. 68%). The unintentional injuries of different age group were varied,for example,the common causes of 1 to 3 years old group were burns /scalds and for-eign bodies(in the esophagus,airway,etc),and the main injuries in 6 to 14 years old group were fall/drop and blunt force. Furthermore, the proportion of unintentional injuries in rural children was 1547 cases (67. 67%),and in the urban children was 739 cases(32. 33%). Unintentional injury with serious conse-quences included burns/scalds 55 cases(26. 96%),sharp object injury 45 cases(22. 06%),burns/scalds 26 cases (12. 75%),blast injury of 23 cases(11. 27%) and fall/drop 20 cases(9. 80%). Disable/sequel hap-pened in 204 cases(8. 92%). Ten cases died(0. 44%) including fall/drop in 4 cases,traffic accident in 3 cases(30%). Conclusion Young children aged 1 to 3 years are the most vulnerable to unintentional inju-ries. Burns/scalds and foreign bodies( in the esophagus,airway,etc) are the most common causes of injuries, and the boys are more common than girls. Moreover,the incidence rate of unintentional trauma in rural chil-dren was higher than that in the urban children. Unintentional injury complicated serious consequences are burns/scalds,sharp object injury,blast injury and fall/drop. Death cases in the majority are fall/drop and traf-fic accident. The key to reduce the occurrence of unintentional injuries is to strengthen propaganda of preven-tion knowledge about the children′s unintentional injuries.

Objective To explore the relationship between subclinical hypothyroidism(SCH)and microvascular complications(diabetic nephrop-athy and diabetic retinopathy)in type 2 diabetic patients. Methods According to the condition of complicating with SCH,157 patients with type 2 diabetes were divided into SCH group(40 cases)and the euthyroid group(117 cases). The age,sex,duration of diabetes,smoking status,blood pressure,body mass index(BMI),glycosylated hemoglobin(HbA1c),blood lipid,renal function,thyroid function,urine albumin/urine creatinine, and fundus examination were analyzed and compared between the two groups. The relationship between SCH and microvascular complications in type 2 diabetic patients was explored. Results There was no significant difference of age,sex,duration of diabetes,smoking,blood pressure,BMI, HbA1c,blood lipid,or serum creatinine between the two groups. The incidence of diabetic retinopathy in SCH group was significantly higher than those in euthyriod group(32.5%vs 14.5%,P=0.044);however there was no difference in the incidence of diabetic nephropathy. After adjustment for potential confounding factors(age,sex,duration,blood pressure,BMI,HbA1c,diabetic nephropathy)by multivariate logistic regression analy-sis,SCH was still the risk factor of diabetic retinopathy(OR=2.210,95%CI 1.512-4.117,P=0.032)in type 2 diabetic patients. Conclusion SCH could be a risk factor of diabetic retinopathy in type 2 diabetic patients,and there was no significant effect on diabetic nephropathy.

Multi-drug resistance of breast cancer remains a major obstacle for effective treatment,which involves many complicated mechanisms,including drug transport in the body,metabolism and drug targets.Recent researches find that the tradition Chinese medicine not only has good effects in improving the body resistance and general situation of patients and enhancing the effects of chemoradiotherapy,but also plays a vital roles in the muti-drug resistance reversal of breast cancer.

BACKGROUND:Fibrin glue can function to close the wound tissue, reduce leakage, stop bleeding, promote wound healing and prevent adhesion.
OBJECTIVE:To systematicaly review the application of fibrin glue in thyroid surgery.
METHODS:We searched China Journal Net database, Wanfang database, VIP database, Chinese Biomedical Database Online to retrieve clinical randomized controled trials related to fibrin glue applied in thyroid surgery from January 2000 to April 2013. Included studies were analyzed using Rev Man 5.2 statistical software for Meta-analysis.
RESULTS AND CONCLUSION:A total of six studies including 797 cases were enroled in result analysis. Meta-analysis showed that the drainage volume at postoperative day 1 and total postoperative drainage volume were higher in the fibrin glue group than the control group (without fibrin glue) [odds ratio=-27.36, 95% confidence interval (-33.86,-20.87),P < 0.00001; weighted mean difference=-38.73, 95% confidence interval (-44.78,-32.67),P < 0.000 01]. The suture removal time was shorter in the fibrin glue group than the control group [odds ratio=-2.00, 95% confidence interval (-2.17,-1.83),P < 0.000 01]. No significant difference was found in the postoperative 3-day fever incidence [odds ratio=1.53, 95% confidence interval (0.59,3.96),P=0.38], wound infection [odds ratio=0.86, 95% confidence interval (0.12, 6.15),P=0.88], and postoperative hematoma [odds ratio=0.86, 95% confidence interval (0.21, 3.48),P=0.83] between the two groups. These findings indicate that fibrin glue used in thyroid surgery can significantly reduce drainage by non-increasing postoperative complications.

Objective To investigate the effect and underlying mechanism of nocodazole on the inhibition of rVSMCs proliferation. Methods rVSMCs were divided into four groups, group A (normal culture), group B (serum-free culture for 24 h) , group C ( 18 h normal culture after 48 h of serum-free culture ) , and group D ( nocodazole treatment for 12 h after thymidine treatment for 12 h) . Flow cytometry, transmission electron microscopy, and metabolism measurements were performed and mitofusin-2 ( Mfn-2 ) expression was detected. Results Flow cytometry analysis showed rVSMCs of group B、C、D were arrested to G0/G1 , S and G2/M phases, respectively. Less and smaller mitochondria were observed in group D by transmission electron microscopy in nocodazole-treated rVSMCs. Compared with groups A and C, there were significant decreases in glucose and L-amino acid metabolism, levels of ATP, and marked increase in NADH in group D(P<0. 05). Western Blot showed that G2/M cell cycle arrest and nocodazole could induce up-regulation of Mfn-2 in rVSMCs(P<0. 05). Conclusion Nocodazole can block the energy metabolism and proliferation in rVSMCs, which is probably associated with the role of Mfn-2 on anti-atherosclerosis.

Objective: To prepare and characterize nobilisideA freeze-dried liposome. Methods: The nobilisideA liposomes were prepared using a film dispersion method followed by a freeze-dried technique. The freeze-dried liposomes were characterized with regard to the drug content, entrapment efficiency, the particle size, ζ potential, and its stability and hemolytic rate in glucose transfusion. Results: Through optimized technique, the prepared nobilisideA freeze-dried liposome, with sucrose as protective agent, had satisfactory structure and redissolubility. The Contents of nobilisideA liposome before and after freeze-drying were 108.3% and 108.6%, the entrapment efficiency were 98.4% and 97.8%, the particle sizes were 89.9 nm and 115.8nm, and the ζ potentials were -19.9mV and -15.9mV, respectively. The liposome content, entrapment efficiency, particle size and hemolytic rate were stable within 8 h during glucose transfusion. Conclusion: NobilisideA freeze-dried liposomes have a narrow size distribution range, a high entrapment efficiency and a stable character, making it a promising candidate for nobilisidesA liposome.

Objective To investigate the therapeutic effect of transplantation of limbal epithelial cells and decellularized porcine cornea construct in rabbits with corneal alkali burn.Methods Allogeneic rabbit limbal epithelial cells were cultured and identified.Porcine cornea scaffold was fabricated by decellularization.The LECs-DPC construct was cultured and transplanted to the alkali-burned corneal surface of rabbits(4month follow-up).Results Immunocytochemistry and RT-PCR analyses revealed the presence of ABCG2 positive limbal stem cells.A general view showed the corneas were clear and vessel-free postoperatively.HE and Masson Trichrome staining results indicated the epithelial stratification,fewer cellular infiltration,DPC' integration into host corneal tissues,and more regular collagen configuration.Transmission electron microscopy exhibited formation of desmosomes and microvilli.Conclusion Allogeneic limbal epithelial cells transplantation using DPC as a carrier can restore corneal clarity after alkali burn in a rabbit model.

Objective To find out the factors which influence the establishment of collateral circulation as well as its clinical characteristics by analyzing the sufferers' medical records.Methods Review and collect the records of the first coronary angiography,and we can find the total occlusion of one coronary after coronary angiography.There are 52 cases in which people who suffered the establishment of collateral circulation stay in hospital for treatment.Here,we take 52 cases in which people didn't suffer into comparison and analyze these clinical records.Results Use the Logistic regression method to analyze the relationship between general clinical data,past history,habits,current medication and the establishment of collateral circulation,which relates to the diabetes mellitus anamnesis,exercise and drinking habits,with the regression coefficient is 0.408,0.483,0.197(P