AIM: To investigate the preoperative binocular visual function of intermittent exotropia and the rebuilding and recovery of the postoperative binocular visual function, and analyze the effect of binocular visual function on orthophoria after surgery.
METHODS:From January 2011 to January 2014, 47 basic intermittent exotropia patients caming for treatment were collected in the clinical data. The changes in their near stereopsis, binocular visual function, binocular fusion and distance stereopsis after operations were recorded in the form of data. The preoperative binocular vision and the postoperative rebuilding were analyzed and contrasted with each other. In addition, the effect on the postoperative maintaining of orthophoria due to the existence, recovery and rebuilding of binocular visual function were observed.
RESULTS:Intermittent exotropia patients got damage in different levels on their binocular visual functions, especially on distance stereopsis, which was the heaviest and earliest. After the operation, all functions were obviously recovered and reconstructed and the improvements were statistically significant compared against those before the operation (P<0. 01). Patients having binocular visual function or part of it before the operation had a higher ratio of orthophoria compared against the patients who had lost binocular visual function before the operation and the difference was statistically significant (P<0. 01). The recovery and reconstruction of the postoperative binocular visual function played an important role in maintaining the orthophoria.
CONCLUSION: The intermittent exotropia cause damage to the stereopsis which happened the earliest. Obvious recovery and reconstruction of binocular visual function can be observed after the surgery. A relatively good preoperative binocular visual function may lead to the increase in the ratio of orthophoria or cure the intermittent exotropia. Performing an operation when distance stereopsis is damaged can increase the success rate for the surgery and reduce the recurrence rate.

Objective To find the key proteins associated with metastasis of ovarian cancer, and find potential diagnostic markers and therapeutic targets of this malignancy. Methods A comparative proteomic strategy, in a combination of two-dimensional electrophoresis separation and mass spectrometry identification, was adopted to search for proteome alternations in an ovarian cancer mother cell line HO-8910 and its highly metastatic cell subline HO-8910PM. Results Twenty-one significantly different spots (two-fold increase or decrease) were detected between the two cell lines, of which 17 candidate proteins were successfully identified and characterized. Compared with those in HO-8910 mother cell line, 16 proteins were significantly up-regulated, while 5 proteins down-regulated in the highly metastatic cell subline HO-8910PM. The seventeen identified proteins could be functionally classified into 7 groups as following: zinc finger protein, calcium-binding protein, DNA repair and synthesis protein, cell regulatory protein, metabolism-related protein, cell surface antigen, cell signals and transducing protein. Conclusions The results suggest that an obviously differential proteomic expression exists between the human ovarian cancer mother cell line HO-8910 and highly metastatic cell subline HO-8910PM. It provides a clue for further identification of metastasis-related proteins, novel diagnostic markers as well as therapeutic targets of this malignancy.

Objective To explore the influences of different inductive methods on cartilage repair by tissue engineered cartilage with the seed cell of mesenchymal stem cells.Methods Rabbit mesenchymal stem cells were divided into dexamethasone-inducing group and TGF-?_1-dexamethasone co-inducing group.The cartilage defects were repaired by autologous tissue engineered cartilage constructs.The defects of control group were filled with scaffold without cells.Specimens were harvested 6 and 12 weeks postoperatively and assessed by histological grading and in situ detection of apoptosis.Results The repair tissue formed perpendicular column structure resembling that of normal cartilage in TGF-?_1-dexamethasone co-inducing group.The histological score 12 weeks postoperatively was higher in co-inducing group(20.26?1.35) than those in dexamethasone-inducing group(14.52?1.46) and control group(4.12?1.13).But the formation of tidemark was not observed in the repair tissue.Cells of repair cartilage at the bone-cartilage interface showed apoptosis.The ratios of apoptosis in dexamethasone-inducing group were(21.4?4.5) six weeks postoperatively and(7.3?2.2) twelve weeks postoperatively.The ratios of apoptosis in TGF-?_1-dexamethasone co-inducing group were(19.8?4.7) six weeks postoperatively and(6.9?2.0) twelve weeks postoperatively.The ratios of apoptosis at 6 th week were higher than those at 12 th week postoperatively with statistical significance(P

Objective To systematically review findings on the effectiveness and safety of transcranial magnetic stimulation (TMS) for treating Parkinson's disease. Methods Foreign and Chinese databases were searched to find relevant trials. The searches were supplemented by searching the reference lists of the published trials. The studies were separated into two groups: those applying TMS at frequencies higher than 1 Hz, and those at 1 Hz or lower. Meta-analysis was performed with the aid of RevMan 5.0 software. Results Both low-frequency and highfrequency TMS can significantly reduce total scores on the Unified Parkinson's Disease Rating Scale (UPDRS) compared with sham stimulations. High-frequency TMS can significantly reduce UPDRS motor scores compared with sham stimulations. On the other hand, low-frequency TMS studies showed no significant overall improvement in motor scores, mental scores or in the activities of daily living (ADL) UPDRS scores. Low-frequency and high-frequency TMS studies also showed no significant overall improvement in Schwab and England ADL scores. Conclusions TMS therapy is safe and partially effective for treating Parkinson's disease.

OBJECTIVE: To establish methods for screening of macromolecules in traditional Chinese medicine injections by molecular exclusion chromatography (SEC). METHODS: The separation was carried out by SEC with Phenomenon BioSep-SEC-s2000 (7.8 mm×300 mm, 8 μm) column. The mobile phase was 0.05 mol·L-1 Na2SO4, and differential detector was used when dextran was used as the reference standard. When proteins were used as reference standards, the mobile phase was the mixture of 0.1% trifluoroacetic acid and acetonitrile (70-30), and UV detector was selected. RESULTS: With the differential detector, the molecular weight range of dextran was 7100-133800, and the limit of detection was 0.0979 μg; with the UV detector, the molecular weight range of the proteins was 1638-13700, and the limit of detection was 0.00358 μg. No macromolecules were detected in the selected traditional Chinese medicine injections. CONCLUSION: The proposed method can be used for the screening of macromolecules in traditional Chinese medicine injections.

Objective To further identify the isolated Mycoplasma genitalium (Mg) from the high risk population of STD in China. Mothods The morphology of Mg standard strains and clinical isolates were observed under electron microscopy (EM) and atomic force microscopy (AFM). Mg sample preparation for AFM was fixed on mica and AFM images of scanning observation were captured by contacting and tapping modes and operated under normal atmospheric pressure and temperature. Results and Conclusion Mg had a diversity of shapes, including flask, pear, spindle or round shape with projecting neck portion and slightly broadened terminal structure under EM. The morphologic features under AFM were similar to those observed by EM. The typical Mg showed shapes of flask and pear with noticeable narrow ring or mark in the neck portion. The size of Mg measured under EM and AFM was similar as well. Mycoplasma

Objective: To learn the concentration of target genes of severe acute respiratory syndrome coronavirus 2 ( SARS-CoV-2 ) and the influencing factors in the confirmed cases with coronavirus disease 2019 ( COVID-19 ) in Ningbo. Methods: Demographic information and clinical diagnosis of COVID-19 cases reported in Ningbo from January 21 to February 20, 2020 were collected through China Disease Control and Prevention Information System. The Ct values of ORF1ab and N of the cases were collected through Ningbo Center for Disease Control and Prevention and designated hospitals, and analyzed in terms of gender, age, severity, clinical classification, source of case, sampling time and specimen type. Results: There were 157 confirmed COVID-19 cases, including 56 males ( 35.67% ) and 101 females ( 64.33% ). Sixty-seven cases ( 42.68% ) aged between 40 and 60 years old. The Ct values of ORF1ab and N in females were 29.96±5.28 and 30.38±4.90, which were higher than 27.56±4.94 and 28.03±4.88 in males ( P<0.05 ). The Ct values of ORF1ab and N were the lowest when sampled at 1-7 days after onset ( P<0.05 ), which were 27.84±4.80 and 28.35±4.65. The Ct values of ORF1ab ( rs=0.288, P=0.001 ) and N ( rs=0.296, P=0.001 ) were positively correlated with sampling time. The Ct values of ORF1ab in pharyngeal swab and sputum were 29.19±4.85 and 28.74±6.40, the Ct values of N in pharyngeal swab and sputum were 29.61±4.60 and 29.22±6.10, both without significant differences between different samples ( P>0.05 ). Conclusions Sampling time has a great influence on the Ct values of ORF1ab and N of SARS-CoV-2.

OBJECTIVE:To provide reference for rational use of anticoagulant drugs in the clinic. METHODS:Medical or-ders of anticoagulant drugs were collected from hospital information system of our hospital during 2013-2015. Those medical orders were analyzed in respects of consumption sum,DDDs,DDC and drug utilization. RESULTS:During 2013-2015,the sum tatio of Low-molecular-weight heparins calcium injection(6000 AXaIU),Enoxaparin sodium injection(6000 AXaIU),Dalteparin sodium injection(5000 IU) and Rivaroxaban tablets(10 mg)were on the rise,while those of Nadroparin calcium infection(6150,4100 AXaIU)and Fondaparinux sodium injection(2.5 mg)were in the decline. DDDs of Nadroparin calcium injection(4100 AXaIU), Dalteparin sodium injection(5000 IU)and Enoxaparin sodium injection(6000 AXaIU)took up the first 3 places. DDC of Rivar-oxaban tablets was the highest,being 167.60-180.25 yuan. The utilization of Nadroparin calcium injection(6150,4100 AXaIU), Low-molecular-weight heparins calcium injection (6000 AXaIU),Fondaparinux sodium injection(2.5 mg) and Warfarin sodium tablets(3 mg)showed good synchronization within 3 years,and the values of B/A ranged 0.50-1.50. Both consumption sum and utili-zation rate of rivaroxaban took up the first place in vascular surgery department. CONCLUSIONS:Based on the spectrum of diseases, the features of drugs and ADR,the utilization of anticoagulant drugs in our hospital is rational during recent 3 years. However,the uti-lization of anticoagulant drugs should be still used as routine pharmaceutical monitoring content.

Objective: To explore myocardial protection effect of artificial subhibernation therapy on rabbits with acute myocardial infarction (AMI) and its possible mechanism. Methods: A total of 24 rabbits were randomly and equally divided into sham operation group, AMI control group and AMI subhibernation group. Changes of their body temperature, heart rate and respiratory frequency were observed. Serum concentration of creatine kinase isoenzyme (CK-MB) was measured by immunosuppression method, and plasma concentration of norepinephrine (NE) was measured by high performance liquid chromatography-electrochemical method. Percentage of myocardial infarct area in left ventricular area (I/LV) and apoptosis index (AI) were compared between two AMI groups. Results: Compared with control group 4 h after establishment of AMI model, there were significant decrease in body temperature [(37.333±0.427)℃ vs. (34.988±0.488)℃], I/LV [(19.281±1.687)% vs. (16.263±2.275)%] and AI [(36.636±1.671)% vs. (34.521±1.356)%], and concentration of CK-MB [(759.863±64.111)U/L vs. (560.225±65.837)U/L] and NE [(200.475±43.581) ng/ml vs. (143.813±43.383) ng/ml] in subhibernation group, P<0.05,or <0.01, but there were no significant changes in heart rate and respiratory frequency between two groups. Conclusion: Artificial subhibernation therapy possesses significant myocardial protection effect on rabbits with acute myocardial infarction through reducing stress response, inducing low temperature and inhibiting cell apoptosis etc.

Objective To analyze the expression level of CD44v6 in small cell lung cancer and the concentration of vascular endothelial growth factor(VEGF) in serum,to explore the clinical significance of them in small cell lung cancer.Methods The expression level of CD44v6 was detected by immunohistochemical methods in 80 cases of small cell lung cancer as patients group,who were treat in People''s Hospital of Jiangyin from January 2014 to January 2016.Another 80 cases of healthy subjects as control group.Used ELISA method to detect the concentration of VEGF of the two groups of patients in the blood.Analyzed the relationship between VEGF,CD44v6 with the survival time of patients.Results The CD44v6 expression in small cell lung cancer was positive in 6 cases,the total positive expression rate was 7.5%.The serum levels of VEGF in patients group((490.9±342.7) ng/L) was significantly different from that in the control group((180.8±67.5) ng/L),and the difference was statistically significant(P=0.024).There was no correlation between the survival time and the expression of CD44v6 in small cell lung cancer(P=0.723).The survival time of small cell lung cancer was negatively correlated with the concentration of VEGF in serum.Conclusion There is a significant correlation between serum VEGF concentration and survival time in patients with small cell lung cancer,which is one of the prognostic factors in patients with small cell lung cancer.