Diagnosis, Differential ; Erythema ; diagnosis ; etiology ; pathology ; Female ; Humans ; Infant, Newborn ; Infant, Premature ; Skin ; blood supply

Objective:To observe the effect of dezocine preemptive analgesia on infraclavicular brachial plexus block and the im-pact of it on postoperative analgesia.Methods:Sixty patients,who were classified by American Society of Anesthesiologists (ASA)criteria as classⅠ-Ⅱ and underwent infraclavicular brachial plexus block,were divided randomly into three groups, with 20 cases in each group.In Group A,5 mg dezocine was combined with local anesthetics(20 mL 0.375 % ropivacaine,15 mL 1% lidocaine and 1mL 1∶200 000 epinephrine)for infraclavicular brachial plexus block;in Group B,5mg dezocine was in-travenously injected 15 minutes before the operation and then the same local anesthetics were used;in Group C,infraclavicular brachial plexus block was performed only with the same local anesthetics.The onset time of anesthesia and the operation time, the visual analogue scale (VAS)at 1 h after anesthesia and 8,12,24,36,48 h after operation and the postoperative analgesia time(the time from the end point of operation to the time point when VAS>3),as well as the side effects were recorded.Re-sults:The onset time of anesthesia in Group A were shorter than that in Group B and C(P <0.05).The VAS at 8 h after operation in Group A and B were significantly lower than that in Group C(P <0.05).The VAS at postoperative 12 and 24 h in Group A were significantly lower than those in Group B and C.There was no statistically significant difference in VAS score at 1 h after anesthesia and at 4,36,48 h after operation among the three groups(P >0.05).The analgesia duration was gradually decreased in Group A ,B and C(P <0.05).There was no statistically significant difference between Group A and B in the in-cidence of side effects.Conclusions:The dezocine preemptive analgesia has exact effect.It could prolong the duration of posto-perative analgesia and has few side effects.

The etiology of deep vein thrombosis (DVT) is still not elucidated nowadays. Based on the accordance between DVT incidence and the anemophilous pollen concentration in the air, we proposed the hypothesis that allergic reaction induced by anemophilous pollen may cause "idiopathic" DVT, and proinflammatory factors may play an important role in the thrombosis process.

Objective To explore the disruption of circadian rhythm induced by the alteration of il?lumination leads to cognition impairement and mood dysreguation in mice. Methods 36 male C57BL/6 mice were divided into constant light group( CL) ,normal light group( N) and constant darkness group( CD) . Open field test was applied for the comparison of locomotor activity,tail suspension test and forced swimming test were conducted for assessment of mood state,and elevated plus?maze and Morris water maze were con?ducted for assessment of cognitive function. Results ( 1) Different circadian rhythms did not change the lo?comotor activity among three groups (CL:(200 160.00±955.28)cm,N:(208 148.00±578.11)cm,CD:(179 128.00±1 185.80)cm, P>0.05). (2) Compared with the control group,CL and CD mice showed significantly decreased immobility time in both TST (CD:(40.16±3.82)s,N:(18.83±2.27)s,CL:(46.00±2.80)s, P<0.01) and FST(CD:(181.33±9.03)s,N:(118.83±7.68)s,CL:(151.83±3.06)s, P<0.05). (3) Compared with the control group,CL and CD mice spent less time (CD:(21.76±6.88)s,N:(80.67±11.19)s,CL:(12.50±5.23)s, P<0.05) and made fewer entries (CD:3.33±0.49,N:6.83±0.91,CL:2.00±0.77, P<0.05) into open arms in elevated plus?maze, and exhibited less crossings in target quarter in Morris water maze (CL:2.67±0.76,N:5.00±0.26,CD:2.83±0.40, P<0.05). Conclusion Chronic constant light or darkness leads to negative impacts on mood and cognition in mice.

Ebolavirus can cause hemorrhagic fever in humans with a mortality rate of 50%-90%. Currently, no approved vaccines and antiviral therapies are available. Human TIM1 is considered as an attachment factor for EBOV, enhancing viral infection through interaction with PS located on the viral envelope. However, reasons underlying the preferable usage of hTIM-1, but not other PS binding receptors by filovirus, remain unknown. We firstly demonstrated a direct interaction between hTIM-1 and EBOV GP in vitro and determined the crystal structures of the Ig V domains of hTIM-1 and hTIM-4. The binding region in hTIM-1 to EBOV GP was mapped by chimeras and mutation assays, which were designed based on structural analysis. Pseudovirion infection assays performed using hTIM-1 and its homologs as well as point mutants verified the location of the GP binding site and the importance of EBOV GP-hTIM-1 interaction in EBOV cellular entry.
Ebolavirus ; metabolism ; Flow Cytometry ; Glycoproteins ; metabolism ; Hepatitis A Virus Cellular Receptor 1 ; Hepatitis A Virus Cellular Receptor 2 ; Humans ; Membrane Glycoproteins ; metabolism ; Membrane Proteins ; metabolism ; Protein Binding ; Receptors, Virus ; metabolism ; Surface Plasmon Resonance ; Viral Envelope Proteins ; metabolism ; Viral Proteins ; metabolism

OBJECTIVETo explore possible correlations between renal Th1/Th2 ratio and renal microvascular injury in children with Henoch-Sch-nlein purpura nephritis (HSPN).

METHODSThirty-two children with HSPN were enrolled. They were classified into four groups by renal pathology: HSPN class II (n=8), HSPN class IIIa (n=7), HSPN class IIIb (n=10) and HSPN class IV/V (n=7). Five patients undergoing nephrectomy due to trauma were used as the controls. INFγ, IL-4 and CD34 in the renal tissues were measured by immunohistochemical analysis. INFγ was used as a marker of Th1, IL-4 was used as a marker of Th2 and CD34 was used as a marker of microvessel. The renal microvessel density was evaluated according to the Weidner standard. The relationships among the local Th1/Th2 ratio, renal pathological grade, microvessel score and microvessel density were studied.

RESULTSImmunohistochemical analysis showed a lower expression of INFγ and a higher expression of IL-4 in the HSPN groups than in the control group. The local Th1/Th2 ratio in the HSPN groups decreased and correlated significantly with the renal pathological grade. There were significant differences among four HSPN subgroups (P<0.05). Compared with the control group, the renal microvessel density in the HSPN class II and class IIIa groups increased significantly (P<0.05), but it decreased in the HSPN class IV/V group (P<0.05). The renal microvessel scores in the HSPN class IIIa, class IIIb and class IV/V groups increased significantly compared with those in the control and the HSPN classⅡ. The increased renal microvessel scores were associated with more severe renal pathological changes. A negative correlation was found between the local Th1/Th2 ratio and the microvessel density in kidneys (r=-0.921, P<0.01).

CONCLUSIONSThe decrease of Th1/Th2 ratio in kidneys might be responsible for renal microvascular injury in children with HSPN.

Adolescent ; Child ; Child, Preschool ; Female ; Humans ; Kidney ; blood supply ; pathology ; Male ; Microvessels ; pathology ; Nephritis ; immunology ; pathology ; Purpura, Schoenlein-Henoch ; immunology ; pathology ; Th1 Cells ; immunology ; Th2 Cells ; immunology

The etiology of deep vein thrombosis (DVT) is still not elucidated nowadays.Based on the accordance between DVT incidence and the anemophilous pollen concentration in the air,we proposed the hypothesis that allergic reaction induced by anemophilous pollen may cause “idiopathic” DVT,and proinflammatory factors may play an important role in the thrombosis process.

Objective To investigate the knowledge, attitude, behavior about Dietary Guidelines for Chinese (2007) among elderly residents in 2 communities in Wenzhou City, and to exoplore the appropriate method for dietary intervention for elderly people. Methods A questionnaire survey was carried out in 784 people aged 50 years and above in two communities in Wenzhou from December, 2014 to January, 2015 about Dietary Guidelines for Chinese (2007) .The awareness of knowledge, attitude and behavior about nutrition were recorded, and multiple linear regressions were taken to evaluate the impression of knowledge. Results The awareness rate of knowledge was 58.28%, and the awareness about 'Which is the healthiest way to cook?' was the hot question (89.29%) while 'Which disease can be prevented by eating iodized salt?' was not (0.89%) . And 53.06% of respondents interested in nutrient knowledge, and 14.54% of them thought they had learned the knowledge about nutrition, and 39.54% of them thought knowledge had impact on health, and 76.53% of them would like to change their unhealthy dietary behavior. About 50.89% of respondents got knowledge about nutrition from TV or radio programs. Intake frequency about vegetables and fish were 88.01% and 62.63%respectively, while intake frequency about milk and milk products and beans and their products were 21.43% and 15.43%respectively. Multiple linear regression promoted that, female, a high degree of education, less number of offspring, people who had married, people whose income came from social insurance had positive impact on the awareness rate of knowledge. Conclusion The awareness rate of knowledge among elderly people was low, and intake frequency about milk and milk products and beans and their products were efficient. But they would like to change their unhealthy behavior. Long-term education about nutrition should be taken for elderly people.

Objective:To study the expression and significance of microparticles (MPs), platelets microparticles (PMPs), CD41a +CD62P +PMPs from peripheral blood of patients with ankylosing spondylitis. Methods:Plasma were collected from 47 ankylosing spondylitis (AS) patients and 15 healthy volunteers. The levels of MPs, PMPs, and CD41a +CD62P +PMPs in plasma of AS patients and healthy controls (HC) were detected by flow cytometry. The clinical parameters including C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), Bath ankylosing spondylitis disease activity index (BASDAI) were obtained. T test and Spearman linear correlation analysis were used for statistical analysis. Results:The levels of MPs, PMPs, CD41a + CD62P + PMPs in plasma from AS patients were higher than those in plasma from HC [(3 466±641)/μl vs (619±152)/μl, t=2.342, P=0.022; (3 059±628)/μl vs (320±143)/μl, t=2.298, P=0.025; (566±121)/μl vs (47±22)个/μl, t=2.295, P=0.025]. The levels of MPs in plasma of AS patients were positively correlated with BASDAI ( r=0.555, P=0.000 4); and the levels of PMPs in AS patients were positively correlated with BASDAI ( r=0.542, P=0.000 6) but the CD41a +CD62P +PMPs in AS patients were not correlated with BASDAI ( r=0.298, P=0.073 2). The levels of MPs in plasma from AS patients were not correlated with ESR, CRP ( r=-0.016, P=0.917; r=0.035, P=0.817); PMPs in plasma from AS patients were not correlated with ESR, CRP ( r=-0.001, P=0.996; r=0.065, P=0.671). CD41a +CD62P +PMPs in plasma of AS patients were not correlated with ESR, CRP ( r=-0.129, P=0.400; r=-0.410, P=0.789). Conclusion:There is increased expression of MPs, PMPs and CD41a +CD62P +PMPs in AS patients, which is related with disease activity. MPs, PMPs may be involved in the inflammatory response of AS.