Objective To evaluate the expression and clinical significance of cyclin dependent kinase (CDK)-activating kinase (CAK) complex including CDK7, cyclin H and accessory protein menage a trios 1 (MAT1) in estrogen receptor-positive breast cancer.Methods A total of 40 patients with estrogen receptor-positive breast cancer from Department of Galactophore, Baoji Maternal and Child Care Service Centre of Shaanxi Province were investigated in this study.Breast cancer tissues and adjacent normal tissues were obtained from patients undergoing surgery.The mRNA expressions of CDK7, cyclin H and MAT1 in two types of tissues were measured by real-time fluorescent quantitative (qRT)-PCR, and their correlations with clinicopathologic features of patients were analyzed.Results The expressions of CDK7, cyclin H and MAT1 in cancer tissues were 2.54±0.78, 2.21±0.56 and 2.46±0.58, while those in adjacent normal tissues were 1.26±0.30, 1.16±0.42 and 1.17±0.39, and there were significantly differences between different types of tissues (t=9.654, P<0.001;t=9.433, P<0.001;t=11.741, P<0.001).The higher expressions of cyclin H and MAT1 in patients′ cancer tissues had the lower clinical stage, with significant correlations (U=3.17, P=0.01;U=2.53, P=0.01).In addition, the tumors were smaller in patients with higher expression levels of MAT1 (χ2=14.16, P=0.01).Conclusion The expressions of CAK complex CDK7, cyclin H and MAT1 are elevated in estrogen receptor-positive breast cancer patients.Cyclin H and MAT1 are closely associated with clinical grade, and MAT1 is also significantly associated with tumor size.

Objective:To explore the relationship of perfection of middle school students and parental rearing behavior.Methods:392 middle school students were investigated by FM PS and CFMPS.Results:①There was a significant difference in OR between different gender(t=-3.039,P

Objective To observe the toxicity and effection of three dimensional conformal radiation therapy(3DCRT)based on conventional fractionation radiotherapy in combination with chemotherapy in non-small-cell lung cancer(NSCLC).Methods 62 NSCLC patients with stage Ⅲ were chosen as research objects.All the patients received chemotherapy two cycles with NP regimen firstly(NVB 40 mg d1,8,DDP 30 mg d2-5),then they accepted radiotherapy in twentith day after chemotherapy when hemocyte got normal.In the first half period of the radiotherapy,conventional radiotherapy were given with 2 Gy/f,after the dose got 40 Gy/20 f,then three dimensional conformal radiation therapy were given with 4～6 Gy/f every 1 to 2 days until the total dose got 24～30 Gy/4～6 f.The patients with supraclavicular lymphnode metastatic carcinoma received conventional fractionation radiotherapy with 60Co or 6 MV-X ray combined electronic ray until the dose(DT)up to 64～66 Gy.After radiotherapy,the patients accepted chemotherapy two cycles with NP regimen again.Results All the 62 patients completed treatment plan,and follow-up survey had lasted for 3 years.The short-term efficacy was evaluated in 3 months after radiotherapy. Among 62 patients, 9 cases gained a complete remission(CR),40 cases partial remission(PR),and the total remission was 79.0%.The acute toxicity showed acute radiation esophagitis,radiation pneumonia and acute gastrointestinal reaction.However,expectant treatment was effective.The 1,2,3 year survival rate was 7 1.0%,48.4%,30.6%,respectively.The 1,2,3 year local control rate was 80.6%,62.9%,40.3%,respectively.Conclusion 3DCRT based on conventional fractionation radiotherapy for NSCLC have good effection and mild toxicity.

Objective To evaluate the effect of dose-rate and leaf position tolerance on the point dose in step-and-shoot intensity-modulated radiotherapy(IMRT). Methods IMRT plans of 2 prostate cancer patients were selected and recalculated for a water phantom.An ionization chamber was used to measure the dose of some points in the phantom at five nominal dose-rates:100 MU/min,200 MU/min,300 MU/min,400 MU/min and 500 MU/min.It was necessary to adiust the position of the water phantom to locate the ionization chamber in region where the dose gradient was very low in order to minimize the effect of dose gradient on the measured results.When measuring the effect of leaf position tolerance on point dose,the dose-rate was kept constant and the values of tolerance were 1 mm,2 mm,3 mm and 4 mm.This work was conducted on a Varian 23EX equipped with a Millennium 120-leaf multi-leat collimator(MLC).The treatment planning system was Varian Eclipse. Results As the dose-rate increased,the error between the measured dose and the calculated dose also enlarged.The difference between the maximum and the minimum was 1.2%.When MLC control system was working normally,tlle effect of leaf position tolerance on the measured point dose was very little and negligible. Conclusions The dose-rate must be selected suitably to ensure that the delivery can be finished in a short time with the radiobiological effect taking into account.It should be noted that the error between the measured dose and the calculated dose increases with the trend of inereasing more rapidly at higher dose rates.The value of leaf position tolerance should not be set too large,in order to minimize the difference between the measured dose and the planned dose in the region of steep gradient and find as early as possible when the multi-leaf collimator control system performs improperly.

Objective To investigate the effect of mucolytic agents during endoscopic sinus surgery.Methods 84 patients of chronic rhinosinusitis were employed in the study,which were randomly divided into the control group(41 subjects) and study group(43 subjects) according to the digital table.The patients in the control group adopted regular therapies,and study group were received regular therapies as well as mucolytic agents(eucalyptol-limonene-pinene soft capsule,300 mg,three times a day) application for 8 weeks.Followed visiting lasted for 6 months.The VAS scores,Lund-Mackay scores and efficient rate were compared.Results The VAS scores and Lund -Mackay scores in the control group after treatment were (3.63 ± 1.71)points and (8.34 ±4.68) points,and which in study group were (2.03 ± 1.67) points and (6.48 ± 3.59) points,and the difference was statistic significant (t =4.338 and t =2.049,P ＜ 0.05).According to CPOS-2012,in the control group,19 cases were controlled,14 cases were partly controlled,and 8 cases were uncontrolled,the whole efficient rate was 80.5％.in the study group,24 cases were controlled,17 cases were partly controlled,and 2 cases were uncontrolled,the whole efficient rate was 95.3％.Compared to the control group,the efficient rate of the study group was higher,the result showed statistical significance (x2 =4.420,P ＜ 0.05).Conclusion Using mucolytic agent after surgery can improve the controlled rate and affect the success of treatment of chronic rhinosinusitis.

ObjectiveTo determine a measurement standard of irregular scar area by comparing the ad-vantages and disadvantages of different measurement methods in measuring same irregular scar area. MethodsIrregular scar area was scanned by digital scanning and measured by coordinate reading method, AutoCAD pixel method, Photoshop lasso pixel method, Photoshop magic bar filled pixel method and Foxit PDF reading software, and some aspects of these methods such as measurement time, repeata-bility, whether could be recorded and whether could be traced were compared and analyzed.Results There was no significant difference in the scar areas by the measurement methods above. However, there was statistical difference in the measurement time and repeatability by one or multi performers and only Foxit PDF reading software could be traced back.ConclusionThe methods above can be used for measuring scar area, but each one has its advantages and disadvantages. It is necessary to develop new measurement software for forensic identification.

Humans ; Leukemia, Large Granular Lymphocytic

Objective To investigate and analyze the effect of current doctor-patient relationship on profession recognition of medical students and to make some accommodations for medical education. Methods Questionnaire was conducted among 800 medical students in North Sichuan Medical College concerning the aspects of recognition of doctor-patient relationship,profession recognition and effect of current doctor-patient relationship on profession recognition . Data statistics was done by SPSS 19 . 0 . Differences were analyzed by frequency,chi-square test by α=0.05 standard and Spearman correlation analysis. Results 67.6%(536/793)of the surveyed students thought that the current doctor-patient re-lationship exerted effect on their job concepts. According to the results of Spearman correlation analysis between current doctor-patient relationship(X)and effect of current doctor-patient relationship on future job con-cepts(Y),correlation coefficient was 0 . 088 ,P=0 . 013<0 . 05 . Conclusions Effect of current doctor-patient relationship on profession recognition of medical students results from health care policy, school education and society,therefore,university should improve related education .

Objective To investigate principles of acupoint selection for the ancient acupuncture and moxibustion treatment of facial paralysis.Methods The ancient literature about acupuncture and moxibustion was collected and reviewed from the pre-Qin period to the late Qing Dynasty. According to the data selection criteria, acupuncture and moxibustion prescriptions of facial paralysis were collected and input into an ancient acupuncture and moxibustion prescription database. By use of frequency analysis and factor analysis, the principles of acupoint selection were obtained.ResultsTotally 257 prescriptions were collected which involve 74 acupoints. Through frequency analysis, 19 acupoints were obtained whose frequencies were equal or greater than 9, such as Dicang (ST 4), Jiache (ST 6), Tinghui (GB 2), Shuigou (GV 26), Hegu (LI 4), etc. Through factor analysis of acupoint variables, 6 common factors were obtained.Conclusion Based on analysis of the common factors, the principles of acupoint selection are summarized which were according to meridian, part and etiology for the ancient acupuncture treatment of facial paralysis. The results could provide a reference for clinical acupuncture and moxibustion treatment. It is feasible that factor analysis was applied to study principles of acupoint selection for acupuncture and moxibustion prescriptions.

Objective To explore the expression and significance of CD44 gastric cancer stem cell biomarker positive circulating tumor cells (CTC ) in peripheral blood of patients with gastric cancer . Methods From March 2010 to December 2012 ,45 patients with gastric cancer and 20 healthy individuals were enrolled .Peripheral blood samples of all the subjects were collected .The expression of CTC and CD44 postive CTC were detected by density gradient centrifugation method and double immunofluorescent staining .Chi‐square test and t‐test were performed for statistical analysis .Results CTC in peripheral blood of healthy controls were not detected .Among 45 patients with gastric cancer ,CTC were detected in 27 patients and 19 of them were CD44‐positive .The difference in positive rate of CTC expression was statistically significant between two groups (χ2 =10 .986 , P<0 .05) .Among 27 CTC‐positive patients , 19 were proximal gastric cancer and 16 of them were CD44‐positive;16 patients had lymph node metastasis and 14 of them were CD44‐positive;13 patients had distant metastasis and 12 of them were CD44‐positive;14 patients had recurrence and 13 of them were CD44‐positive .The CD44 positive CTC was correlated with tumor location ,lymph node metastasis ,distant metastasis and recurrence (χ2=5 .891 ,5 .527 ,5 .787 and 7 .052 ,respectively ,all P<0 .05) .Furthermore ,in 13 patients with recurrence and CD44 positive CTC ,the recurrence time was (10 .54 ± 5 .55) months which was shorter than that of one patients with CD44 negative CTC (19 months) ,and the difference was statistically significant (t= -3 .654 , P=0 .004) .Conclusions CTC which express cancer stem cell marker may be associated with recurrence and metastasis of gastric cancer .It has more important clinical significance to detect this subtype of CTC .