Objective To investigate the nalaxone treating first acute right heart failure.Methods52 patients with FARHF were divided into the naloxone group(30cases)and control group(20cases)at random.Patients in control group were given general comprehensively treating.Patients in naloxone group were given naloxone as well as comprehensively treating,were given intravenously 0.8 mg of first dose,if it was necessary,repeated 0.8 mg of dose,and continuously dropped for 1.2 mg was added into liquid 500 mL.After treatment of 24 hours,were compared the changes of HR、SBP、SV and heart function.ResultsIn the naloxone group,the total effective rates were 84.4%,the mortality rates were 6.6%;in control group.The total effective rates were 60%,the mortality rates were 18.3%.The difference of the effective rate of the naloxone group be treatment was statistically significant(P

Objective To develop a double antibody sandwich ELISA for quantitatively detecting Japanese encephalitis virus(JEV) antigen.Methods The anti-JEV polyclonal antibodies were used to coat ELISA plates.Anti-JEV monoclonal antibodies were used as enzyme-labeled conjugate.A standard curve based on known amounts of JEP antigen was established by the ELISA.Various parameters of the assay were analyzed.Results The optimal linear range was 12.5～200 U/ml(r=0.9989).The quantitation limit was 12.5 U/ml.The recovery rate for the accuracy test was 85.0%～103.3%.The coefficients of variation for intra-assay and inter-assay precision were 4.3%and 5.5%respectively.No cross-reaction was observed with HAV vaccine,influenza vaccine,Vero cell Iysates,newborn bovine serum,or human albumin.Conclusions The data indicate that the ELISA developed in this study has high specificity,precision, accuracy,and stability.The assay should be suitable for quantitative determination of JEV antigen in various vaccine products.

Objective To explore the preliminary practice of Clinical Scenario Mode in the teaching of surgery. Methods Clinical Scenario Mode was stimulated or rebuilt by the cooperation of teachers and Standardized Patients (SP) to introduce relative theories and raise discussions in the surgery lectures of five-year clinical medicine students in grade 2004 and grade 2005. Results Lectures with the application of Clinical Scenario Mode were reported positively by students from the survey. It improved students’learning motivation,enhanced teaching quality,and optimized the allocation of resources. Discussion With the combination of the advantages of lecture based learning (LBL) and problem based learning (PBL),Clinical Scenario Mode can be considered as the linkage to the widespread use of PBL,and should be widely applied in the medical teaching practice.

Audiometry, Speech ; Congresses as Topic

Objective To investigate the effect of HP1γ on the development of ovarian cancer. Methods RT-qPCR was used to deteched the level of HP1γin ovarian cancer samples. The proliferation of A2780 and SKOV3 cell line was detected by CCK8 assay. RNAi was used to knock down the expression of HP1γ mRNA. Results The level of HP1γwas higher in ovarian cancer than that in the normal tissue. Mir-30b may inhibit the proliferation of ovarian cancer cell. Conclusion HP1γpromotes the proliferation and progress of ovarian cancer cells.

CK.Efficiency of Myo decreases apparently after heart accident onset is more than 6 hours.Traditional marker,CK,CK-MB,AST,LDH and HBDH still have some efficiency in AMI diagnosis.

Aim To explore the correlationship of allergy to penicillin and HLA-DRB genetic polymorphism.Methods Sequence specific primer-polymerase chain reaction(PCR-SSP)was used to type for human leucocyte antigen-DBR(HLA-DRB)alleles in 77 patients with allergy to penicillin and 87 healthy subjects without any allergic reactions.Results Compared with control subjects,a significantly increased frequency of DR9 was present in 77 patients with allergic reactions(11.04% vs 4.02%,RR=3.24,P

Objective To study on the relationship between insulin resistance and thyroid nodule in the patients of normal glucose metabolism. Methods The clinical data of 105 patients of normal glucose metabolism were analyzed. The patients were divided into nodules group (54 patients) and non- nodules group (51 patients). The age, sex, body mass index (BMI), fasting plasma glucose (FPG), postprandial 2 h blood glucose (2 h PG), fasting insulin (FINS), postprandial 2 h (2 h INS), fasting C peptide (FC-P), postprandial 2 h C peptide (2 h C-P), glycosylated hemoglobin (HbA1c), blood uric acid (BUA), homeostatic model assessment insulin resistance index (HOMA-IR) were compared between two groups. According the level of HOMA-IR, 105 patients were divided into different subgroups, the detection rate of thyroid nodules was compared. The influencing factors of thyroid nodules were analyzed by multi-factor Logistic regression analysis. Results The age and sex between two groups had no significant differences (P>0.05). The levels of BMI, BUA, HOMA-IR, FPG, FINS, 2 h INS, FC-P, 2 h C-P and HbA1c in nodules group were significantly higher than those in non-nodules group (P<0.05). The detection rate of thyroid nodules in HOMA-IR 3. 01- 4.00 group (12/14) and HOMA-IR > 4.00 group (20/20) was higher than that in HOMA-IR 0- 1.00 group (0), HOMA-IR 1.01- 2.00 group (39.5%, 17/43) and HOMA-IR 2.01-3.00 group (5/17), there were significant differences (P<0.01). Multi-factor Logistic regression analysis showed that the detection rate of thyroid nodules had positive correlation with levels of HOMA-IR, BUA and BMI (OR=0.278, 0.004 and 0.115, 95%CI 1.695-5.043, 1.001-1.015 and 1.046-1.644). The detection rate of thyroid nodules had no correlation with level of FPG (P>0.05). Conclusions The detection rate of thyroid nodules in HOMA-IR>3.00 patients is significantly higher. The insulin resistance, higher BMI and hyperuricemia are the independent risk factors of thyroid nodules.

Objective To understand the side-effects of cyclophosphamide (CTX) in the treatment of systemic autoimmune diseases and the possible risk factors. Methods Two hundred and forty-one patients with systemic autoimmune diseases were recruited from the Rheumatology Division of Peking University First Hospital during January 1st, 2009 and March 31, 2012. All the patients received oral or intravenous cyclopho-sphamide. The data were collected by medical record review as well as telephone follow-up. Logistic regression analyses were used for statistical analysis. Results Statistical analysis for age, sex, disease, cumulative dose, treatment duration and mode of administration were included in the factor analysis that would impact the CTX related side-effects. Age ( x2=14.8, P=0.002), gender ( x2=11.2, P=0.001), the underlying disease ( x2=26.1, P<0.01), cumulative dose ( x2=9.8, P=0.007) and mode of administration of CTX ( x2=19.5, P<0.01) were all correlated with the incidence of CTX side-effects. Multivariate analysis showed that women [OR=2.32, 95%CI (1.15, 4.70), P=0.02], intravenous-oral sequential use of CTX [OR=5.25, 95%CI (2.30, 11.97), P<0.01] and systemic lupus erythematosus [OR=4.02, 95%CI (2.24, 7.21), P<0.01] as the underlying disease were independent risk factors for CTX side-effects. Conclusion Alopecia, gastrointestinal discomfort and gonads toxicity ware com-monly seen in Chinese patients with systemic autoimmune diseases receiving CTX. Hemor-rhagic cystitis is very rare. Women, intravenous-oral sequential use of CTX and the systemic lupus erythematosus are indepen-dent risk factors for CTX side-effects.

Objective:To investigate influence of living locations on the personality characteristics of the only child and none-only children in an air force academy.Methods:1921 cadets were tested by the Chinese version of the MBTI-G.Results:Scores of the only child cadets from cities on the Sensing-Intuition and Thinking-Feeling dimensions were higher than those of none-only children group from cities and those of the only child group from countries.The scores of them on the SN scale were 93.0?18.5,90.4?17.6 and 90.1?17.7(t=2.25,P=0.025;t=1.99,P= 0.048).The scores of them on the TF scale were 94.6?22.6,89.1?21.1 and 88.4?21.0(t=4.02,P