The army hospital is an important component of the national health resource and its par- ticipation in the reform of the civilian health program is a big step to strengthen the construction of the army Hospital,an action to prevent the army from wasting limited health resource,a measure to ease the pressure put by local governments on civilian health service.The health service rendered by the army is a tie to keep the army in close contact with the people.Therefore,the social service done by the army hospi- tal has an exceptional significance beyond the ordinary sense of medical service.Its participation in the re- form of the civilian health program is of positive meaning in raising its capability either in time of peace or in time of war.The army hospital has technical advantages,tt has a good name both in its service attitude and medical morality.It tends to lower the medical cost through the improvement of administration.All those mentioned above are important conditions for its participation in the reform of the civilian health program.

Organ transplant the patient be special mental state of regulate, the budget consume a control, organ resources a development exploitation and transplant a performance evaluation is current check and supervision human body organ transplant the work open an exhibition of key problem. Need the angle which learns from the ethics, the knowledge which uses science strengthens to cure the communication for suffer from and strengthen the confidence that the sufferer cure and use to publicly line up with the realistic case of the sufferer's equilibrium mindset of the method maintenance resolve mental state to regulate a problem; The thought which learns with the ethics examines closely sufferer's psychological stress and learn from the ethics of the angle be excellent to turn a profect to lower expenses and use publicly in procession sufferer's equilibrium mindset of the method maintenance; Paww the social foundation of extensive organ source of the publicity extension and insistobtaining maintenance ethics to learn a principle and insistin a medical science by lqw the standard insures the science exploitation of organ; Pay attention to the transplantation performance of the method evaluation of use the economicsand pay attention to pay attention to resolve the transplantation performance of the corpse organ to maximize a problem towards live body organ the risk performance of the transplantation evaluate with, help the clinical organ to transplant a smoothly open of the work an exhibition.

Case material search system is mainly used in the rapid search,tracing and location of field medical cases including all material in it.It can timely determine the location and volume of medical material when they are in transportation,airdrop and mobile outspread so as to improve the rescue support ability for the wounded.

Literature of TCM contains abundant information of Chinese medicine. Traditional Chinese medicine information is characterized by its high degree of knowledge-intensive and complex inter-knowledge, so it is difficult to integrate and share knowledge. TheShanghanlun is one of the Chinese medical classics, and it includes the rules and methods of syndrome differentiation and treatment and it showed good effects in the clinical application. However, it is difficult to master for beginners. As a kind of knowledge organization tool, topic maps can flexibly define the relationship between knowledge, which realizes the visualization of knowledge network, and integrates and shares the distributed knowledge. This paper focused on the current situation of topic maps researches. The topic maps was used to organize the original content ofShanghanlun, construct the concept model of it and explore the specific application of topic maps technology in the field of traditional Chinese medicine.

Objective：To study the ultrastructure changes of osteosarcoma cells(OS-732) inactivated by alcohol of two different concentrations and clinical application value.Methods：Osteosarcoma cells(OS-732) were inactivated by 75% and 95% alcohol and observed by light and electron microscope.And its viability was detected by MTT method. Results：Cell internal structure changed significantly and irreversible damage formed. MTT method proved that inactivated cells had no viability.Conclusion：These two inactivation methods were effective. Cell internal enviroment was damaged very seriously and cell was led to death. These two inactivation methods could provide choices for clinical limb protective operation.

Objective To explore the effects of semiconductor laser on proliferation of the multidrug-resistant model of human osteosarcoma cell line(ROS-732).Methods Four test groups and one control group were set up.The test groups were divided into A,B,C and D groups according to the irradiation time(output power was 200 mW).The time of laser irradiation of A,B,C and D groups were 5,10,20 and 40 min,repectively.The control group(E) wasn′t treated with laser irradiation.MTT method was used to observe the proliferation of R-OS-732,and the morphological changes of the cells were observed by inverted microscope.Results The survival rates of cells in all test groups under condition of semiconductor laser irradiation with 200 mW output power and 532 nm weavlength,compared with control group(P

Objective To evaluate the effects of pyruvate Ringer' s solution on erythrocyte deformation function in the patients undergoing cardiopulmonary bypass (CPB) in vitro.Methods Thirteen patients of both sexes,aged 25-64 yr,of ASA physical status Ⅱ or Ⅲ (NAHY Ⅱ or Ⅲ),scheduled for elective cardiac valve replacement under CPB were enrolled in the study.At 10 and 60 min of CPB (T1,2) and 60 min after termination of CPB (T3),blood samples 18 ml were drawn from the radial artery and each patient's blood sample was equally divided into 6 parts (3 ml per part).The blood samples were divided into 3 groups (n =13 each):control group (group C),lactated Ringer's solution group (group R),and pyruvate Ringer's solution group (group P).In C,R and P groups,normal saline 1 ml,lactated Ringer's solution 1 ml,and pyruvate Ringer's solution 1 ml were added,respectively,and then the erythrocytes were incubated for 60 min at 37 ℃.At 30 and 60 min of incubation,the erythrocyte rigidity index (ERI) and erythrocyte deformation index (EDI) were detected.Results Compared with C and R groups,the ERI was significantly decreased at 30 and 60 min of incubation at T1,the EDI was decreased at 30 min of incubation at T1,2,and no significant change was found in ERI at T2,3 or in EDI at T3 in group P.There was no significant difference in ERI and EDI at different time points of incubation within groups.Conclusion Pyruvate Ringer's solution can enhance erythrocyte deformation function in the patients undergoing CPB.

Most studies related to determining the expression profile of genes and specific promoters used histochemical localization of the reporter gene, gusA. While the histochemical method for visualizing gusA expression suffers from several limitations in the determination of gene expression and location, especially in the tissues with high background acitivty. To solve this problem, a transient expession vector pBI221-RFP/GFP, was constructed using GFP and RFP as double fluorescent marker genes. This vector used CaMV 35S promoter to drive GFP and determine the transforming efficiency. It analyzed expression profile of the target gene and promoter through the RFP activities of the tranformed tissues. Through the specific promoter AGPL1 from watermelon and E8 promoter from tomato, it is resistible to use this vector to study the expression patterns of promoters. Results indicated that the pBI221-RFP/GFP is a very efficient transient expression vector that can be verify the functions of the genes and promoters.
Citrullus ; genetics ; Gene Expression Regulation, Plant ; Genes, Plant ; Genes, Reporter ; Genetic Vectors ; genetics ; Green Fluorescent Proteins ; genetics ; metabolism ; Luminescent Agents ; metabolism ; Luminescent Proteins ; genetics ; metabolism ; Lycopersicon esculentum ; genetics ; Promoter Regions, Genetic

OBJECTIVE: To improve the results of the treatment of unstable types of intertrochanteric fractures of the femur (ITFF) by internal fixation. METHODS: A L-trapezoid compression plate system (L-TCP) was designed and compared to the AO/ASIF angle plates of 95 and 130 (L-AP) with mechanical tests. An Evans Type III model was made, the simulation electrical test with loading on single and both legs were done. The results demonstrated that the stability of the L-TCP fixation was significantly greater, and the stress concentration at the upper screw hole was relevantly smaller than that of L-AP. From June 1986 to June 1998, 82 patients of ITFF were treated with L-TCP fixation. The average age was 67.4 years old (26-91). Seventy-four of those fractures were unstable. Fifty-eight patients were treated with open reduction, and 24 cases were treated by closed reduction and limited open (only lateral aspect of upper femur was exposed) fixation with L-TCP under X-ray monitoring. RESULTS: Sixty-six patients were followed up for 6-69 months. Complications, including 3 wound infection, 1 non-union, 3 coxa varus and 2 implant breakage (9 complications of 7 cases), all occurred in the open reduction group. No complication was found in the closed reduction group. CONCLUSIONS: In the treatment of ITFF, the results of closed reduction and limited open fixation with L-TCP is significantly better than that of open reduction and fully exposed fixation.