In recent years, new targets research of Anti-HIV drugs brings in new hope for the development of Anti-HIV drags. Based on Science Citation Index Expanded, the paper analyzes the research focus, country distribution and research institution distribution of new targets of anti-HIV drugs by Bibliometrics.

More than 100 countries have added hepatitis B vaccination to their routine immunization programmes.Hepatitis B vaccines progressed from blood vaccines to recombinant vaccines,then to peptide vaccines and DNA vaccines,to their functions extended from prophylactic to therapeutic.This article analyzes the relationship between hepatitis B vaccine R&D and the immunization programmes in different countries,to summarizes the R&D of both prophylactic and therapeutic vaccines,and points out the direction for futare vaccine development.

Objective To explore the expression of Excision repair cross -completion 1 in non-small cell lung cancer and to analyze its clinical significance .Methods ERCC1 protein expression was detected by IHC method in 160 cases of non-small cell lung cancer tissue .We analyzed relationship between the ERCC 1 ex-pression and clinicopathological parameters of non -small cell lung cancer ,as well as its correlation between ER-CC1 expression and the prognosis .At the same time ,160 cases of intraoperative specimens were primarily cul-tured.The relationship between ERCC1 expression and the drug sensitivity of DDP was analyzed .Results ER-CC1 protein was mainly expressed in the nucleus .ERCC1 protein expression in lung cancer tissue showed no rela-tionship with age,gender,tumor size and pathological types .ERCC1 protein expression was associated with TNM stages and lymph node metastases .High expression of ERCC1was significantly correlated with poor prognosis in pa-tients,indicating that ERCC1 is the independent factor of NSCLC patients .MTT results showed that the positive ex-pression rate of ERCC1 in DDP sensitive groups was higher than in DDP resistant groups .Conclusion ERCC1 is an independent factor influencing the prognosis of non -small cell lung cancer ,and associated with DDP resistance .

Objective To explore the differentia expression of long chain noncoding RNA (IncRNA) in peripheral blood mononuclear cells (PBMCs) of the patients with rheumatoid arthritis (RA) and normal human and to analyze to further clarify the pathogenesis of RA.Methods Twenty-two cases of RA in our hospital from January to April 2016 were selected as the observation group and 22 healthy persons served as the control group.The differential expression of IncRNA and mRNA in PBMCs of 5 RA cases and 5 healthy persons were determined by using Agilent human IncRNA chip;GO and Pathway were used to analyze the functional distribution of differentially expressed IncRNA,the co-expression pressionsnetwork was constructed and the Trans-and Cis -were adopted to predict the RA onset related IncRNA.Results There were 1623 differential expressions of IncRNA in PBMCs of RA patients and healthy persons and 851 differential expressions of mRNA.GO and Pathway analysis showed that the main function of differentially expressed mRNA was to participate in the binding and transcriptional regulation of protein kinase,nucleotide and metal ions,and also participate in the regulation of B cell receptor signaling pathway and TNF signaling pathway.After Cis-and Trans-analysis prediction and real-time PCR validation,the 3 IneRNA could be related with RA onset,which were NONHSAT 120696,uc.80+ and NONHSAT 031501.Conclusion IncRNA has differential expression in PBMCs of RA patients and healthy persons,three 031501 IncRNA of NONHSAT 120696,uc.80+,NONHSAT may be related to the pathogenesis of RA.

Leucine zipper tumor suppressor 2 (LZTS2) is a novel tumor suppressor gene that has been increasingly recognized in recent years. Currently, many studies illustrate that LZTS2 gene, the important candidate tumor suppressor gene, is already involved in the inhibition of tumorigenesis and aberrant proliferation of tumor cells, and other functions of tumor cells. Information from these stud-ies can contribute to the formulation of new strategies for the treatment of tumors.

0.05).The transfected HepG2 cells by infection of SL3261 containing the vector were shown with GFP and the 930 bp target expression by RT-PCR.Conclusion:Attenuated Salmonella typhimurium SL3261 containing recombined eukaryotic expressing plasmid pIRES2-EGFP-4-1BBL is successfully constructed which can deliver recombinant plasmid into HepG2 cells.

Objective To investigate genetic relationships among five serotypes of two variants of Cryptococcus neoformans. Methods PCR mediated DGGE (denaturing gradient gel electrophoresis) and sequence analysis of 28S rDNA of C. neoformans were performed in ten reference strains, C. neoformans capsular-deficient strain CAP10, and nineteen clinical isolates from non-HIV patients. Results The results of DGGE and analysis of nucleotide sequences of 28S rDNA showed identical patterns and nucleotide sequences in the serotype A and D of C. neoformans var. neoformans, which were distinct from the serotye B and C of C. neoformans var. gattii. The patterns and sequences of serotype AD coincided with those of C. neoformans var. gattii. The patterns and nucleotide sequences of C. neoformans capsular-deficient strain CAP10 (serotype D) and serotype A and D were identical. Of the nineteen clinical isolates, seventeen had patterns of serotype A and D, and the others had patterns of serotype B and C. Conclusions PCR mediated DGGE integrated with sequence analysis of 28S rDNA is a valuable tool for the classification of C. neoformans. The clinical isolate of C. neoformans var. neoformans is predominant in Chinese non-HIV patients. Serotype AD is genetically close to C. neoformans var. gattii rather than C. neoformans var. neoformans. The data seem not to be in favor of previous study that serotype A, C. neoformans var. grubii, is a new variant of C. neoformans.

Objective To evaluate the value of CT-guided biopsy of deep-located lesion in the diagnosis of lymphoma. Methods CT-guided percutaneous biopsy was performed in 58 patients with 16-20 gauge core-needle biopsy. The locations of lesion involved mediastinun, lung, retroperitonurn, spleen, kidney, adrenalal and musculoskeletal system. Pathology examination included hematoxylin and eosin staining and immunohistochemical assays. Results In 56 out of 58 cases,the biopsy findings could be confirmed by histologic examination including correctly diagnosed 47 malignant lymphomas, corresponding to a sensitivity of 81%;with fuithecmace subclassification 42 of the 47(89.4%) could be as diagnosed malignant lymphomas on the basis of CT-guided biopsy. Conclusions Biopsy of deeply located lymphoma mass under CT guidance has high diagnostic accuracy and low complication rate with convenience for subclassification of malignant lymphomas.

Objective To study the technique and clinical significance of percutaneous mediastinum biopsy guided by CT. Methods 35 cases with mediastinal masses were undergone this procedure. Results The successful rate of the procedure was 100%. The successful punctural rates reached 100% for malignant neoplasms(21/21)85.71% for benign lesions(11/14), and the diagnostic accuracy was 94.21%(33/35). The complication of pneumothorax occurred in 3 cases, and mediastinal bleeding presented in 1 case. Conclusions CT-guided percutaneous mediastinum biopsy possesses high diagnostic accuracy, simultaneously with great help for the management.

Objective To assess the reliability and useness of using a coupling device for end-to-end venous anastomosis in patients undergoing free-tissue transfer in head and neck reconstruction.Methods Twelve patients undergoing surgical resection and head and neck defects were repaired with fibular flap,anterolateral thigh flap and radial forearm flap.The microvascular anastomotic device was used to perform venous anastomosis in these cases.Flap survival and thrombosis of the venous anastomoses were determined.Results There were no flap losses due to venous thrombosis in this series.The venous anastomosis was usually coupled in about 5.3 minutes and appear as an obviously time savings compared with performing a hand-sewn venous anastomosis.Conclusion The microvascular coupler is excellent in end-to-end venous anastomosis and certainly less taxing on the surgeon when compared with standard suture techniques.