To study the clinicopathological and immunohistochemical characteristics of omental and mesenteric stromal tumor, 8 cases of stromal tumor were investigated, and followed up. The results showed that these tumors were solitary nodule, most were large. Histologically, cytoplasmic eosinophilia and perinuclear vacuolization were often seen in omental and mesenteric stromal tumor of spindle cell type. 3 cases showed epithelioid cytologic features. An immunohistochemical study showed that all the patients were C kit positive (100%),7 were CD34 positive, whereas 6 were SMA positive, positive expression of p53 and PCNA was found in 2 cases of malignant stromal tumor, Lower expression of PCNA was observed in 1 case of benign one.The results suggested that omental and mesenteric stromal tumors have distinct clinicopathological features, the expression of C kit and CD34 plays an important role in its diagnosis.

Objective To investigate the significance of the mutation of c-kit gene in the development,(clinical) pathology and prognosis of gastrointestinal stromal tumors(GISTs).Methods The technique of(PCR-SSCP) was used to detect ckit gene mutation in 106 cases of GIST,and 57 of the cases were(followed-up).Results The c-kit gene mutation rate in the whole group was 45.3%(48/106),and(3.4)%(2/41) in benign GIST,and 70.8%(46/65) in malignant GIST.The 1-,3 and 5-year survival rates and median survival time of the mutation positive group were all markedlly lower than those of the(mutation) negative group.A comparison of the parameters that can differentiate the benign GISTs from malignant ones such as size,histological necrosis,aggressive grade,nucleus mitotic count and nuclear discrepancy,showed significant difference between the of postive and negative gene mutation(P

Objective: To explore the sensitivity of Kit or PDGFRA mutants related to gastrointestinal stromal tumor (GIST) to Gleevec.Methods: The recombinant plasmids of KIT Del559-560, KIT Ins IPYD579, PDGFRA D842V and PDG-FRA L839P gene mutants were transiently transformed into the CHO cells by liposome methods.Western blot was used to detect the expression of the related protein and their phosphorylated forms after the cells were incubated with Gleevec for 90 min.At 72 hours after incubation with Gleevec, MTT was used to detect cell proliferation.Results: Western blot results showed that Gleevec at 0.1 μM can notably reduce phosphorylation of KIT Del559-560.Gleevec at 1μM completely blocked phosphorylation of KIT Ins IPYD579 and PDGFRA L839P, but did not affect PDGFRA D842V phosphorylation.MTT analy-sis indicated that growth of CHOPDGFRA L839P was inhibited by Gleevec at 1μM, however, CHOPDGFRA D842V was re-sistant to Gleevec at 5 μM.Conclusion: Gleevec can decrease the expression of phosphorylated protein CHOPDGFRA L839P and CHOKIT Ins IPYD579, and can remarkably inhibit the proliferation of cells containing PDGFRA L839P mutant.

Objective: To study the expression of CD15 mRNA,CD44v6 mRNA and nm23H1 mRNA in colon carcinoma and its relationship with clinical pathological parameters and prognosis of colon carcinoma. Methods: In situ hybridization was used to detect the expression of CD15 mRNA, CD44v6 mRNA and nm23H1 mRNA in 90 cases of colon carcinoma, and 53 cases were followed up for more than 5 years. Results: In 90 cases of colon carcinoma, the expression of CD15 mRNA,CD44v6 mRNA and nm23H1 mRNA were 84.4%,68.9%,66.7%, respectively. The high level expression of CD15 mRNA, CD44v6 mRNA and the low level expression of nm23H1 mRNA were positively corelated with the Dukes staging,serosa infiltration,lymph node and liver metastasis of colon carcinoma.The expression of CD15 mRNA in colon carcinoma was positively associated with that of CD44v6 mRNA and negatively with that of nm23H1 mRNA. Conclusion: CD15 mRNA,CD44v6 mRNA and nm23H1 mRNA play an up-regulation and a down-regulation role, respectively, and have a synergistic action in metastasis of colon carcinoma. CD15 mRNA can be used as a new biological index to predict the invasive potential of colon carcinoma and objectively evaluate the prognosis of patients.

Objective: To observe the effect of coxsackie virus B3 on airway tract and lung morphology, and to study the relation between CVB infection and asthma. Methods: We established CVB3 infective model: 5 d neonatal rats inhaled CVB3 by ultrasonic brume. CVB3-IgM was examined 10 d after inoculating of CVB3, and LW/BW, airway tract and lung pathological change 10 d and 30 d after inoculation of CVB3 were observed. Results: Rats from the virus group had higher D of CVB3-IgM than control's (+2s ) and had higher LW/BW 10 d after inoculation of CVB3 than control (P<0.01). Neonatal rats had acute inflammatory changes 10 d after inoculation of CVB3 and persistent changes in morphology and cytology. Conclusion: Neonatal rats virus model is established. Respiratory infection by CVB3 in neonatal rats has persistent changes in airway tract inflammatory and morphology.

Objective: To observe the effect of coxsackie virus B 3 on airway tract and lung morphology, and to study the relation between CVB infection and asthma. Methods: We established CVB 3 infective model: 5 d neonatal rats inhaled CVB 3 by ultrasonic brume. CVB 3 IgM was examined 10 d after inoculating of CVB 3, and LW/BW, airway tract and lung pathological change 10 d and 30 d after inoculation of CVB 3 were observed. Results: Rats from the virus group had higher D of CVB 3 IgM than control's ( +2s ) and had higher LW/BW 10 d after inoculation of CVB 3 than control ( P

Formalin-fixed paraffin-embedded biopsy specimens of the normal nasal mucosa,nasal polyps, inverted papillomas and papillary adenocarcinomas were analyzed by Avidin Biotin-Peroxidase Complextechnique for the demonstration of lectin receptors including peanut agglutinin (PNA), concanavalin ensifomis agglutinin (ConA),ulex europeaus agglutinin-I (UEA-1), sophora japonica agglutinin (SJA), wheat germ agglutinin (WGA), pisum sativum agglutinin (PSA), carcinoembryonic antigen (CEA) and keratin.lt was found that the distribution of PNA and UEA-I receptors was related to the degree of differentiation, dysplasia or malignant transformation of the nasal mucosa. The quantity of ConA receptors was increased with the degree of transformation of the nasal mucosal cells. There were many kinds of lectin receptors on the surface of one kind of cells. Lectin histochemistry was more economical, convenient, rapid and sensitive than immunohistochemistry. So, lectin histochemistry can be used to examine the degree of differentiation, dysplasia and malignant transformation of nasal neoplastic cells, providing objective index for clinical diagnosis, treatment and prognosis.

Objective To analyse the difference of IgG expressions between fetal and adult rabbit skin in wound healing for exploring the mechanisms of fetal-type and adult-type wound healing. Methods Based on a fetal rabbit wound healing model, we extracted IgG from normal and wounded tissues using the homogenization method and determined its value using ELISA technique in the comparative study. Results We found that the variation of IgG showed a tendency of elevation with the prolonging of pregnancy in normal rabbits(23-28 days gestation);the IgG value in adult rabbits was much higher than that of the fetuses(P＜0.01);IgG value in wounded fetuses was higher(P＜0.01) than that of corresponding normal fetuses during the first two days after wound creation, and there were no differences between wounded fetuses at different gestation times. From the 2nd day of wound creation, IgG value in wounded adults was much higher than the normal(P＜0.01) and had an obvious tendency of elevation. All the facts suggested that the immunological system became mature with the development of the fetus. Despite the lower IgG levels in the fetus,it also manifested some immunological reactions to injury whereas the adult took intense reactions with higher IgG value and an obvious tendency of elevation. Conclsion The difference of immunoreaction is one of the main reasons of the fetal-type and adult-type wound healin

Objective To analyse the difference of IgG expressions between fetal and adult rabbit skin in wound healing for exploring the mechanisms of fetal-type and adult-type wound healing. Methods Based on a fetal rabbit wound healing model, we extracted IgG from normal and wounded tissues using the homogenization method and determined its value using ELISA technique in the comparative study. Results We found that the variation of IgG showed a tendency of elevation with the prolonging of pregnancy in normal rabbits(23-28 days gestation);the IgG value in adult rabbits was much higher than that of the fetuses(P＜0.01);IgG value in wounded fetuses was higher(P＜0.01) than that of corresponding normal fetuses during the first two days after wound creation, and there were no differences between wounded fetuses at different gestation times. From the 2nd day of wound creation, IgG value in wounded adults was much higher than the normal(P＜0.01) and had an obvious tendency of elevation. All the facts suggested that the immunological system became mature with the development of the fetus. Despite the lower IgG levels in the fetus,it also manifested some immunological reactions to injury whereas the adult took intense reactions with higher IgG value and an obvious tendency of elevation. Conclsion The difference of immunoreaction is one of the main reasons of the fetal-type and adult-type wound healin