AIM: To investigate differentiation of CD34~+ cells in human umbilical blood into eosinophils under the condition of cell culture in vitro.METHODS: CD34~+ cells were separated and purified from human umbilical blood.The cells were divided into negative group,IL-5 group and allergic rhinitis serum group.The differentiation ability of the cells was measured by flow cytometry,HE staining and electron microscope at the first day,second day, 7th day,14th day and 28th day culture.RESULTS: The proportion of CD34~+ cells in IL-5 group and allergic rhinitis serum group were decreased at the second day.The proportion in allergic rhinitis serum group was lower than that in IL-5 group significantly.The typical structure of eosinophils was observed at the second day.CONCLUSION: The allergic patient serum and IL-5 induce differentiation of CD34~+ cells in human umbilical blood to eosinophils.

Doctor-Patient knowledge transfer plays an important role in clinical service.In an evidence-based investigation on their basic features and preference,patients investigated fall into six categories according four influence factors.These factors comprise,set as the criteria,information preference,decision-making preference,perceived severity of disease and influence of reference group.The six groups are the contradiction group,reactive group,ego group,decision preference group,total equality group,and positive group.Doctor-Patient knowledge transfer stategiea are suggested based on the preference features of the six groups.These strategies serve as references and paramedics for doctors to perform effective doctor-patient communication.

Doctor-patient trust plays an important role in doctor-patient knowledge transfer.Investigating the basic features and trust performance of the patients, the authors classified by means of a cluster analysis, the patients into four groups with such criteria as the trust on hospital, trust on doctors, patient's awareness of the knowledge, and patient's compliance to the doctor's advice. The four groups are rational and suspicious, passive compliance, high self-confidence, and blind trust groups. In view of behavioral characteristics of the patients, different doctor-patient knowledge transfer strategies are suggested, in an effort to perform effective doctor-patient communication and to gain patient's trust.

Objective To compare the differences between doctor knowledge transfer willingness and patient knowledge transfer demand,and propose matching behavior model of Doctor-Patient knowledge transfer.Methods Useing empirical analysis through questionnaire survey,tests and verities investigate data's reliability,validity.Significant test of the differences between doctor knowledge transfer willingness and patient knowledge transfer demand is conducted with ANOVA,and the detail relationship is conducted by comparison of the average values.Results Patient knowledge transfer demand is much higher than doctor knowledge transfer willingness.What' s more,this paper divides content of Doctor Patient knowledge transfer into four kinds:high doctor intention-high patient demand,high doctor intention-low patient demand,low doctor intention-high patient demand,low doctor intention-low patient demand.Conclusions Mismatching of doctor knowledge transfer willingness and patient knowledge transfer demand is the main reason of medical information asymmetry.Doctor-Patient knowledge transfer strategies are suggested based on the performance features of the four groups.

98%. Conclusion This method was suitable for the determination of trace arsenic in cosmetics.

Objective To compare the effects of autologous blood transfusion and allogenic blood transfusion on the patients undergoing selective operation of intervertebral fusion with cage. Methods Forty patients who underwent selective operation of intervertebral fusion with cage in the De?partment of Orthopedics of our hospital from September 2012 to June 2014 were recruited for the study,including 20 cases that received autologous blood transfusion only(group A)and 20 cases that received allogenic blood transfusion only(group B). Preoperative and postoperative results of blood routine examination,body temperature,postoperative recovery indicators and expense of blood transfusion were compared between the two groups. Results The postoperative erythrocyte and hemoglobin of the patients in group A were significantly higher than those in group B(P<0.05). When the amount of blood used during the operation reaches or exceeds 4 units,the expense of autologous blood transfusion was lower than that of allogenic blood transfusion. Conclusion Autologous blood transfusion contributes to higher levels of postoperative erythrocyte and hemoglo?bin. When a large amount of blood is used during an operation,autotransfusion can help to reduce the expense of blood transfusion.

Objective To explore the differences of presonal backgrounds of patients on their perceived value and service strategy for such patients.Methods Based on a literature review,the survey data of patient perceived value of ten hospitals from 2011 to 2013 were selected for an analysis of typical influences on their perceived value incurred by their background characteristics.Results The patient perceived values of the highest attention were functional value(3.91 in average)and emotional value(3.92 in average);regular changes toward these two values were found among patients of different backgrounds.Comparatively patients tend to demand higher emotional value featuring outpatient visit,minor symptoms,females,youths,higher education background and high income.Conclusions Medical institutions are expected to clarify their value positioning,and provide based on such differentiated services to their patients in view of the characteristic backgrounds and medical visits,thus improving the perceived value of their patient population in general.

Objective To observe the effect of aerobics exercise on myocardial fibrosis after acute myocardial infarction (AMI) in rat.Methods Twenty-four AMI Sprague Dawley (SD) rats were randomly assigned to sham-operated group (Sham),AMI group and aerobics exercise treatment group (ET).Except Sham,other groups of rats were underwent anterior wall myocardial infarction.After ten weeks,the myocardial mRNA level of connective tissue growth factor (CTGF),collagen Ⅰ (COL1 a1),collagen Ⅲ (COL3a1) detected by real time-quantitative polymerase chain reaction (PCR) analysis and myocardial collagen volume fraction (CVF) was determined on Masson stained sections.Results Campared to Sham-operated group,AMI group the level of CTGF,COL1a1,COL3a1,and CVF were increased (P ＜0.05,P ＜ 0.01).Campared to AMI and ET groups,the levels of CTGF,COL1a1,COL3a1,and CVF were decreased (P ＜ 0.05,P ＜ 0.01).Conclusions Aerobics exercise can reduced the myocardial fibrosis after AMI in rat.

By means of literature review, theoretical analysis and analogy analysis, the authors analyzed the characteristics and risk exposure of online medical information transmission. According to whether the sender has a clear intention or target and whether the receiver intends to search,the transmission models of such information fall into four types: "clear-active", "unclear-active", "unclear-passive", and"clear-passive". The characteristics of online medical information transmission include the complexity of multiple dimensions and multiple levels, multiple sources of information, multiple directions and media of transmission, and the rapid development of mobile internet medical information technology. The risk exposure of online medical information transmission can be classified into three categories: the risk of information dissemination source, that of transmission process and that of receiver′s individual behavior. Transmission risks mostly come from quality gaps of information dissemination sources, uncontrollable transmission paths, numerous influencing factors, difficulties in information screening and poor regulating systems.

0.05), while the average size of nucleolus at the cervical enlargement was relatively larger than that at the thoracic segments (P