Objective To observe the clinical effect of recombinant bovine basic fibroblast growth factor gel combined with esberitox for recurrent oral ulcer.Methods Forty-six patients with recurrent oral ulcer were divided into treatment group of 22 patients and control group of 24 patients by random digits table method.The patients in treatment group were given recombinant bovine basic fibroblast growth factor gel and esberitox.The patients in control group were given the watermelon frost spray only.After treatment for 1 week,the curative effect was compared.Results The cure rate in treatment group was 90.9％ (20/22),which was significantly higher than that in control group (66.7％,16/24),and there was significant difference (P ＜0.05).The time of pain vanish,oral ulcer decrease,oral ulcer healing in treatment group were significantly shorter than those in control group,and there were significant differences (P ＜0.05).Conclusions Recombinant bovine basic fibroblast growth factor gel combined with esberitox for recurrent oral ulcer can promote ulcer healing,shorten the course of disease,and has no adverse reaction.It is worthy of applying in clinic.

Objective To evaluate the value of acute physiology and chronic health condition evaluation Ⅱ(APACHEⅡ) and National Institute of Health Stroke Scale(NIHSS) score in evaluating outcome of patients with acute cerebral infarction(ACI).Methods 399 patients with ACI were scored by APACHEⅡ and NIHSS in 3 d after onset.Prognosis judgement accorded to the patients survival or death during 28 d after onset.Area under of the receiver operator characteristic(ROC) curves was used to scale the ability of APACHEⅡ and NIHSS scoring systems evaluating severity and predicting outcomes of patients with ACI to find best cut-off points of the two scoring systems.Using discriminant analysis method to analysis the two scoring systems and estabished mathematical functional equation to calculate accuracy.Results For prognosis of patients with ACI,the areas under ROC curves were 0.835 and 0.822,and the best cut-off points were 12 and 10 respectively for APACHEⅡand NIHSS.The accuracy rates of APACHEⅡand NIHSS were 74.5% and 76.9%,respectively.The accuracy rate of APACHEⅡcombined NIHSS was 85.5%.Conclusions The APACHEⅡand NIHSS score systems have satisfactory discrimination for patients witn ACI,The predictive accuracy may improve if APACHEⅡ is combined with NIHSS.

Objective To investigate the signal transduction pathway of arachidonic acid(AA) and prostaglandin E-2(PGE-2) synthesis in macrophage invaded by Toxoplasma gondii. Methods Synthesis of AA and PGE-2, expression of COX_2 mRNA and protein following stimulation infection by Toxoplasma gondii were evaluated in RAW264^7 cells by ELISA, RT_PCR and Western blotting after treatment with calcium channel blocker verapamil, chelator of extracellular calcium EGTA and inhibitor of CaM trifluoperazine (TFP), selective PKC inhibitor H7. Results Production of AA and PGE-2 induced by tachyzoite was significantly inhibited by EGTA, TFP and BAPTA/AM, and the PGE-2 production was inhibited by H7, with a reduced expression of COX_2 mRNA and protein in a dose_dependent manner. Conclusion The parasite down_regulates macrophage functions by affecting PKC signaling pathways, and triggers a biochemical cascade whose signals ultimately conduct to the secretion of immunosuppressive molecules PGE-2.

Objective:To observe the changes of TLR4 and IL-6 expression in rats hippocampus CA1 region in remote ischemic postconditioning(RIP) and explore its significance.Methods: All the 72 male SD rats were divided into Sham group,Contrast group and RIP group randomly.Each group was divided into 4 time points:12h,24 h,48 h and 72 h group.There were 6 rats in each group.Use the cerebral ischemia-reperfusion model which was established with modified Longa method as the contrast group.The method of RIP was to Fasten rats Posterior limbs by a tourniquet for 30 min immediately,then relax them for 30 min, repeat 3 times.To observe the pathological variation of hippocampus CA1 region by HE dyeing;to test the expression of TLR4 and IL-6 by immunohistochemical staining.Results: Compared to contrast group, neuronal loss and swelling reduced significantly in RIP group.Compared to sham group, the TLR4 and IL-6 expression in contrast group and RIP group increased significantly ( P<0.05 ) .Compared to contrast group,the TLR4 and IL-6 expression in RIP group reduced significantly(P<0.05).Conclusion:RIP dose have protective effect on cerebral ischemia.The effect may be associated with the inhibition of TLR4 and IL-6 expression.

Objective To detect expression of PRKACB protein in non-small cell lung cancer ,thus to study its clinical significance and to analyze the relationship between PRKACB expression and prognosis .Methods We detected the PRKACB protein using immunohistochemistry in 109 lung cancer tissues and corresponding adjacent 20 normal tissues of non-small cell lung cancer patients .Results Compared with normal tissues , the level of PRKACB significantly decreased in lung cancers (χ2 =25.217,P<0.05),and was associated with differentiation (χ2 =9.664,P<0.05),lymph node metastasis(χ2 =4.430,P<0.05),and TNM staging(χ2 =4.430,P<0.05). Furthermore,the low expression of PRKACB protein was correlated with poor prognosis ( overall survival and disease-free survival ) of the patients .Conclusion PRKACB was lowly expressed in non-small cell lung cancer ,and associat-ed with clinical features ,which indicated its important role in clinical diagnosis of non-small cell lung cancer .

OBJECTIVE：To preliminarily study the antitumor mechanism of Periplaneta americana extract C Ⅱ-3 on MFC tumor-bearing mice. METHODS ：Balb/c mice were randomly divided into model group （normal saline 20 mL/kg）and C Ⅱ-3 group （200 mg/kg），with 6 mice in each group. MFC cell suspension （0.2 mL）was injected under the right armpit of mice. On the next day，mice were given relevant medicine intragastrically ，once a day ，for consecutive 10 d. 24 h after the last administration ，Based on the measurement of tumor size ， 1H-NMR technology combined with unsupervised PCA ，supervised PLS-DA and OPLS-DA were used to compare metabolic spectrum of liver tissue from tumor-bearing mice of 2 groups，to analyze differential metabolites and to explore the potential antitumor mechanis m of C Ⅱ -3. RESULTS ：Compared with model group ，the tumor body was significantly reduced in tumor-bearing mice of C Ⅱ-3 group. There were differences in 1H-NMR spectra between the 2 No.81960712）； groups. According to unsupervised PCA ，supervised PLS-DA and OPLS-DA ，totally six potential differential metabolites ，as glycogen （increased），pyruvate （decreased），arginine （de- creased），hydroxyproline （increased），inosine （increased） and niacinamide （increased），were identified in the liver tissue，which were mainly attributed to the metabolism of arginine ，energy and nucleic acid. CONCLUSIONS：The anti tumor effect of C Ⅱ-3 may be related to the regulation of arginine metabolism ，energy metabolism and nucleic acid metabolism.

On October 23, 2017, a 52-year-old male patient with 3 recurrences of dermatofibrosarcoma protuberans in the left shoulder and chest was admitted to the Department of Burns and Plastic Surgery of Dali Bai Autonomous Prefecture People′s Hospital. Dermatofibrosarcoma protuberans on the skin were completely resected, leaving wound defect of 10 cm×10 cm. The wound was planned to be repaired by the transplantation of right anterolateral thigh perforator free flap. However, the anterolateral thigh perforator branch was absent during flap removal, and only one small perforating branch was found. Moreover, it was difficult to separate. Therefore, this flap cutting was given up. The anteromedial thigh perforator was explored through the same incision, and a thicker perforator was found, which was supplied by an independent iatrogenic artery. The length and diameter of the vascular pedicle matched with the blood vessels in the receiving site. An anteromedial thigh perforator flap (10 cm×10 cm) was cut to repair the defect. The postoperative 9-month follow-up revealed that the color, texture, and thickness of the flap were good, the two-point discrimination distance was 30 mm, and the linear scar remained at the donor site of right thigh.

OBJECTIVETo study the infection situation of arbuscular mycorrhizal fungi, as well as the mycorrhizal structures of Paris polyphylla var. yunnanensis, and the main types and quantities of AMF spores in rhizosphere soil.

METHODThe arbuscular mycorrhizal of P. polyphylla var. yunnanensis were detected by Phillips and Hayman staining. At the same time, some AMF spores were accessed by Gendemann's Wet-screening method and identified by their morphological characteristics.

RESULTArbuscular mycorrhizal fungi could infect the roots of P. polyphylla var. yunnanensis and formed arbuscular mycorrhizal. Infection rate was from 35.3% to 98.6%, indicating that infection strength was strong. From 10 soil samples collected in Yunnan, 11 Acaulospor species, 7 Glomus species, 3 Gigaspora species and 3 Scutellospora species were isolated and identified, including Acaulospora appendicola, A. brieticulata, A. excavata, A. foveata, A. lacunosa, A. laevis, A. koskei, A. myriocarpa, A. polonica, A. rehmii, A. scrobiculata, Glomus albidum, G. ambisporum, G. deserticola, G. fragarioides, G. luteum, G. microaggregatum, G. multiforum, Gigaspora albida, G. margarita, G. ramisporophora, Scutellospora calospora, S. pellucida and S. gilmorei. Among them, Acaulospora brieticulata was advantage species.

CONCLUSIONAMF may be a potent biological resource which can stimulate the growth of P. polyphylla var. yunnanensis.

Fungi ; isolation & purification ; physiology ; Magnoliopsida ; growth & development ; microbiology ; Mycorrhizae ; isolation & purification ; physiology ; Plant Roots ; growth & development ; microbiology ; Soil Microbiology

From November 2015 to July 2017, six patients with skin and soft tissue defects of vulva, vagina, and buttock after resection of vulvar tumors were hospitalized in our unit. All patients were female, aged 45-70 years. Among them, four patients had bilateral defects, and two patients had unilateral defect. The defect area on each side ranged from 6 cm×4 cm to 12 cm×6 cm. Internal pudendal artery perforator " angel wing" island flaps were used to repair and reconstruct the defects. The area of flaps ranged from 7 cm×5 cm to 14 cm×7 cm. The donor sites were sutured directly. All 10 flaps of 6 patients survived. Two patients had local incision infection 3 days after operation. One of the two patients was healed 2 weeks after dressing change, and the other one underwent debridement and suture 1 week after dressing change and was healed 1 week after surgery. Follow-up for 6-12 months after surgery showed no recurrence of tumors, no eversion of vagina, better shape of vulva in bilateral reconstruction cases, and slightly worse symmetry in unilateral reconstruction cases. The skin of the reconstructed area was soft, with sensations of pain, temperature, and touch recovered in varying degrees. The distance of a two-point discrimination was 20-30 mm. Linear scars were left in the flap donor sites, with no impact on squatting or striding. In vaginal examination, 1.5 to 2.0 fingers could be inserted in bilateral reconstruction cases, while 2.0 to 3.0 fingers could be inserted in unilateral reconstruction cases. The anus functioned well during defecation.

OBJECTIVETo study the chemical constituents of Swertia mileensis.

METHODThe air-dried whole plants of S. mileensis were extracted with 50% EtOH. The EtOH extract was suspended in H20 and extracted with petroleum ether, CHCl3 and n-BuOH successively. The compounds were isolated and purified by column chromatography from the CHCl3 fraction, and identified based on spectral analyses (MS, H-NMR, 13C-NMR).

RESULTTwelve compounds were isolated from S. mileensis, and were elucidated as 1,5, 8-trihydroxy-3-methoxyxanthone (1), 1-hydroxyl-2, 3, 5, 7-tetramethoxyxanthone (2), 1-hydroxyl-3, 5, 8-trimethoxyxanthone (3), 1-hydroxyl-2, 3, 4, 6-tetramethoxyxanthone (4), 1-hydroxyl-2, 3, 4, 7-tetramethoxyxanthone (5), 1,8-dihydroxy-3, 5-dimethoxyxanthone (6), 1, 7-dihydroxy-3, 8-dimethoxyxanthone (7), 1, 3, 5, 8-tetrahydroxyxanthone (8), balanophonin (9), oleanolic acid (10), maslinic acid (11), sumaresinolic acid (12).

CONCLUSIONCompounds 1, 3, 7-9, 11 and 12 were obtained from S. mileensis for the first time.

Drugs, Chinese Herbal ; chemistry ; isolation & purification ; Swertia ; chemistry