BACKGROUND:Control of hepatic stelate cel activation and proliferation is the focus of developing strategies against liver fibrosis. Human or murine bone marrow mesenchymal stem cels can induce apoptosis of hepatic stelate cels through paracrine of hepatocyte growth factors. OBJECTIVE:To explore the mechanism by which bone marrow mesenchymal stem cels participate in apoptosis of rat hepatic stelate cels. METHODS:Hepatic stelate cels and bone marrow mesenchymal stem cels were seeded and co-cultured in the upper and lower chambers in a co-culture system, serving as a co-culture group. In the blank control group, only hepatic stelate cels were involved. In the c-Met inhibitor group, hepatic stelate cels and bone marrow mesenchymal stem cels were treated with 3 mg/L C-Met inhibitor. In the RhoA inhibitor group, both kinds of cels were treated with 3 mg/L RhoA inhibitor. RESULTS AND CONCLUSION:The concentration of c-Met inhibitor was 3.0 mg/L. RhoA inhibitor at 30μmol/L exhibited a greater inhibitory effect than at other concentrations. RhoA mRNA and protein expression in the co-culture, c-Met inhibitor and in particular RhoA inhibitor groups was obviously greater than in the blank control group. Hepatocyte growth factor concentration in each group was gradualy decreased with time, hepatocyte growth factor activator concentration in each group was gradualy increased with time, and the changes were most obvious in the c-Met inhibitor group. Apoptosis rate of hepatic stelate cels in each group was gradualy increased with time, and highest apoptosis rate appeared in the RhoA inhibitor group, and lowest apoptosis rate in the c-Met inhibitor group. These findings suggest that bone marrow mesenchymal stem cels participate in and promote the apoptosis of hepatic stelate cels by activating hepatocyte growth factors and downregulating Rho activity.

Objective To evaluate the efifcacy and safety of endoscopic submucosal dissection in treatment of prolapse of gastric mucosa.Methods to meet the typical gastric mucosal prolapse of gastric mucosa under endoscopy, the ultrasonic gastroscopy confirmed that the lesion was in the mucosal layer, and the disease was excluded. After PPI continuous treatment for 3 months, no signiifcant improvement in symptoms, in patients with informed consent under the premise of endoscopic mucosal stripping. After 3 months of the operation return visit section for outpatients re-examination of gastroscopy.Results 10 cases of complications of endoscopic submucosal dissection of gastric mucosa prolapse patients had no hemorrhage, perforation after operation, postoperative patients were followed up for 3 months, the symptoms were improved obviously, no antral mucosal prolapse was observed under endoscope.Conclusion Endoscopic submucosal dissection is effective and safe in treatment of gastric mucosal prolapse.

Objective To establish the corresponding relationship between syndrome differentiation of TCM and clinical test results in chronic hepatitis B patients,and thus to provide objective evidence for TCM treatment. Methods 196 patients with chronic hepatitis B,according to the Chinese medicine dialectical typing results were divided into five groups(liver kidney yin deficiency syndrome type 35 cases,Shire Zhongzu syndrome type 24 cases, Yang deficiency of spleen and kidney syndrome type 26 cases,damp heat resistance type and blood stasis syndrome type 42 cases,liver stagnation and spleen deficiency syndrome type 69 cases).HBV -DNA,hepatitis B,TBIL,ALT, TTT were detected.Results In the HBV -DNA test,the majority of the five groups were positive,the overall compar-ison,the difference was not statistically significant (P =0.937).But in the second liver five,TBIL,ALT and TTT detection,the detection results were reflected in the corresponding relationship between TCMsyndrome differentiation. Such as in TTT detection,liver kidney yin deficiency syndrome type and liver stagnation and spleen deficiency syn-drome type group of patients,most patients with TTT detection results between 7 and 10,two groups sequentially com-pared with the other three groups,the differences were statistically significant (all P =0.000);damp heat resistance type and blood stasis type in the patients group,TTT detection results were generally less than 6,two groups in turn compared with the other three groups,the differences were statistically significant (all P =0.000);Yang deficiency of spleen and kidney in the patients group,TTT detection results were generally more than 10,and compared with the other four groups,the differences were statistically significant (all P =0.000).Conclusion TCMsyndrome differen-tiation of chronic hepatitis B patients has a certain correlation with the clinical test results.

Objective To explore the clinical efficacy of tension-free vaginal tape obturator inside-outside(TVT-O)in the treatment of female stress urinary incontinence.Methods In this study,we enrolled the patients with stress urinary incontinence who were treated with TVT-O(n=72)or midodrine hydrochloride(n=72).The patient self-evaluation and the Results of pad test and urodynamic test 6 and 24 months after the treatment were compared.Results These 2 kinds of treatment significantly improved the symptoms in patients with stress urinary incontinence,and in patients treated with TVT-O the clinical efficacy 24 months after the treatment was better.Conclusion TVT-O is safe and effective in treating female stress urinary incontinence,and the long-term follow-up and the improvement of urodynamic indices show it is better than simple drug treatment.

BACKGROUND:As a mixture of several kinds of stem cels and progenitor cels, autologous bone marrow concentrate has been a research hotspot because of the advantages including easy to obtain, time saving and relatively low cost, which can avoid cel extraction, culture and amplification, and can preferably maintain the bioactivity of cels. OBJECTIVE: To review the current basic research and clinical research on bone marrow concentrate in regenerative medicine. METHODS: A computer-based search of CNKI and PubMed databases was performed for articles relevant to bone marrow concentrate published from January 2000 to February 2015. The key words were bone marrow concentrate, bone marrow mesenchymal stem cels, endothelial progenitor cel in Chinese and English, respectively, which appeared in the title and abstract. Finaly, 62 articles were included in result analysis. RESULTS AND CONCLUSION: Bone marrow concentrate can be harvested by density gradient centrifugation technique, with the advantages of easy to obtain and minimal risk of infection and immunological rejection. Currently, the bone marrow concentrate has been proved to be beneficial to wound healing, tissue regeneration and angiogenesis by numerous studies. Meanwhile, it has been successfuly introduced in clinical application. However, studies about the amount of transplantation are stil in need.

【Objective】 To explore the status and characteristics of voluntary blood donors in rural areas of Dali Bai Autonomous Prefecture (referred as Dali), and to provide basis for scientific and effective voluntary blood donation in rural population in regions inhabited by ethnic groups. 【Methods】 The data of rural blood donors who donated blood in Dali from 2010 to 2019 were collected, including demographic data as nationality, gender, age, educational background, as well as the blood donation frequency and blood infection screening (index) results. The above data of urban blood donors who donated blood in Dali during the same period were selected to investigate the increasing trend of blood donation rate. SPSS26.0 was used for statistical analysis of the collected data of rural and urban blood donation population. 【Results】 From 2010 to 2019,the number of blood donors in Dali increased from 13 949 to 19 479,with an increasing rate of 39.64%. The number of rural blood donors increased from 2 623 to 8 727,among which the number of ethnic minority groups increased from 1 779 to 5 059.The ratio of male to female blood donors was 70.30% (1 844/2 623) vs 29.70% (779/2 623) in 2010,56.37% (4 919/8 727) vs 43.63%(3 808/8 727) in 2019. Those with educational level of junior middle school or below were the most, accounted for 43.97%(38 443/85 836),with ethnic donors of 24.47%(23 583/85 836). The proportion of donors aged between 36 and 45 was the highest[40.73% (30 477/74 827) ], with ethnic donors of [28.56% (21 374/74 827), and the proportion of repeated blood donors was 54.87%(35 279/64 299),with ethnic donors of 49.89%(18 080/36 240) [the proportion of repeated blood donors in urban donors in the same period was 48.13% (55 677/115 675) ] (P<0.01). The unqualified rate of ALT was the highest [1.21%(1 272/105 489) ] [The unqualified rate in local donors was 1.99%(3 837/192 552) ] (P<0.01), and that of the ethnic donors was 1.51%(358/56 718). There was no significant difference in the unqualified rates of HB-sAg, anti-HCV, anti-HIV and anti-TP among urban and rural blood donors (P>0.05). 【Conclusion】 It is of great significance to explore the characteristics of blood donors in rural areas (especially regions inhabited by ethnic groups) and the reasons for disqualification, in order to scientifically carry out the recruitment of voluntary blood donors and further promote blood donation for rural residents

Dendritic Cells ; Hematologic Neoplasms ; Humans ; Infant ; Male

Gentiana is an important but complicated group in Gentianaceae. The genus covers numerous medicinal plants which are difficult to be identified. In the present study, several medicinal species in Gentiana from Yunnan province, including G. rigescens, G.rhodantha, and G. delavayi, were sequenced using the Illumina HiSeq 2500 system. Three complete chloroplast genome sequences were obtained after assembly and annotation. According to several published genome sequences of G. crassicaulis, the DNA super-barcoding of species in Gentiana was preliminarily carried out. The results revealed that chloroplast genomes of the three species were conservative with short lengths(146 944, 148 992, and 148 796 bp, respectively). The genomes encoded 114 genes, including 78 protein-coding genes, 30 tRNA genes, 4 rRNA genes, and 2 pseudogenes. Furthermore, these medicinal species in Yunnan province were identified using DNA super-barcoding based on chloroplast genomes. The results showed that the Gentiana species could be gathered into monophyletic branches with a high support value(100%). It indicated that DNA super-barcoding possessed obvious advantages in discriminating species in complicated genera. This study is expected to provide a scientific basis for the identification, utilization, and conservation of Gentiana species.
China ; DNA ; Genome, Chloroplast/genetics* ; Gentiana/genetics* ; Phylogeny

Objective　To observe the growth-associated protein-43(GAP-43),synaptophysin (SYN) and postsynaptic density 95（PSD95） in the amygdala of rats with epilepsy-associated depression (EAD),and to explore the role of these three synaptic-related proteins in the pathogenesis of EAD.Methods　Healthy adult SD rats were randomly divided into comorbidity group,epilepsy group,depression group and normal group.The lithium chloride-pilocarpine method was used to establish the epilepsy model.The epilepsy model was screened for depression 14 days after the model was established.The rats with epilepsy and depression were the comorbid group;the rats without depression were the epilepsy group.The chronic depression model was established in the depression group by chronic unpredictable moderate stress stimulation combined with orphan method.The normal group is healthy SD rats.On the 29th day (4 weeks later) after the successful modeling,the brain was taken out by intravital perfusion and fixation in vivo,and the amygdala was separated from the brain tissue.The immunohistochemical staining was used to detect the expression of GAP43,PSD95 and SYN protein in the amygdala of rats in each group.Results　Compared with the control groups,the number of GAP-43 and PSD95 immunohistochemical positive cells in the comorbid group was the least,and the normal control group was the most (P<0.05).There was no statistical significance in the other groups (P>0.05).Compared with the epilepsy group and the normal group,the SYN immunohistochemical positive cell expression in the comorbid group and depression group was reduced (P<0.05).The epilepsy group was more than that of the depression group,and less than that of the normal group (P<0.05).Conclusion　The decreased expression of GAP-43,SYN,and PSD95 in the amygdala of epileptic rats with depression may be related to the onset of depression in epilepsy rats.

Humans ; Organ Transplantation ; legislation & jurisprudence ; Patient Selection ; Tissue Banks ; legislation & jurisprudence ; organization & administration ; Transplant Recipients