OBJECTIVE:To observe the clinical efficacy of Shengxuening tablets reducing the risk of microinflammation in maintenance hemodialysis(MHD)patients with renal anemia. METHODS:60 MHD patients were randomly divided into observa-tion group and control group with 30 cases in each group. The total dialysis times of all the patients were 12 h every week at blood flow of 200-250 ml/min and dialysis flow of 500 ml/min. Both groups received routine treatment;observation group was additional-ly given Shengxuening tablets orally 1.0 g,tid;control group was given ferrous succinate 0.2 g,tid,orally;both groups were giv-en subcutaneous injection of erythropoietin (EPO),10 000 IU/time,once a week;treatment for all of the patients lasted for 3 months. The fasting venous blood sample were collected at the beginning of treatment and in the morning every one months. The levels of hypersensitive C-reactive protein(hs-CRP),tumor necrosis factor α(TNF-α),IL-6,hemoglobin(Hb),and hematocrit (Hct)were detected. RESULTS:After 3 months of treatment,total effective rate of 2 groups were 90.00%,the levels of hs-CRP、TNF-α、IL-6 in observation group were much lower than that before treatment and control group after treatment,with statistical sig-nificance(P<0.05 or P<0.01). No severe ADR was found in 2 groups,and the incidence of ADR in observation group(6.67%) was significantly lower than in control group (13.33%),with statistical significance (P<0.05). CONCLUSIONS:Shengxuening tablets have good curative effect,and they can reduce the risk of microinflammation due to the long-term use of EPO and chalybeate.

The current mental health of college students can not be optimistic.This paper summarized college students' psychological crisis from the perspectives of psychological crisis and crisis intervention,came up with updated research outcomes and deficiencies,and pointed out the direction of its future research and development.

Objective To investigate the effects of artervenous fistula on structure and function of heart in patients with hemodialysis in the Second Clinical Hospital of Chinese Medical University from 2004 to 2005.Methods AVF、LAD、RVD、LVD、LVEDV、SV and EF were examined and analysed before and 8 weeks after AVF in 55 patients with hemodialysis.Results LAD、RVD、LVD、LVEDV after AVF increased significantly and EF reduced evidently after AVF.Conclusion AVF can cause extension of LAD、 RVD、 LVD and decrease of EF.

Objective To evalute effects of low calcium concentration dialysate on blood pressure,ionized calcium and serum endothelin-1 of uremic patient with hypertension during hemodialysis.Methods 60 uremic patients with hypertension during hemodialysis in the Shengjing Hospital of China Medical University were dialysated with different calcium concentration(1.75 mmol/L followed by 1.25 mmol/L).TO measurearterial blood pressure,ionized calcium and serum endothelin-1 before and after each dialysis session.Results The post-dialysis blood pressure,ionized calcium and serum endothelin-1 level of uremic patients using DCa1.75 with hypertension during hemodialysis were increased significantly than those of pre-dialysis and post-dialysis with DCa1.25(P

BACKGROUND: Foreign and domestic scholars have made some studies on local anatomy and imageology of osseous pterygopalatine fossa, but studies on anatomy of section of osseous pterygopalatine fossa are few.OBJECTIVE: To measure hole-hole distance and aperture of pterygopalatine fossa from adult cranial bone at coronary and horizontal planes, and to observe the morphology of pterygopalatine fossa at corresponding sections.DESIGN: Repeated measurement design.SETTING: Scientific Research Office, Chengdu Medical College.MATERIALS: This experiment was carried out in the Laboratory for Local Anatomy, Department of Human Anatomy,Chengdu Medical College from March to November 2006. Sixty sides of complete dry cranial bone samples from 30 Chinese adult cases, who were of either gender and regardless of age, were involved in this study.METHODS: Bilateral pterygopalatine fossa of 30 dry cranial bone samples (60 sides) were sliced, 15 for slicing at coronary plane, and 15 for slicing at horizontal plane. Morphology of pterygopalatine fossa at the corresponding layers was observed, and related hole-hole distance and aperture were measured. Outcome was performed statistical analysis.MAIN OUTCOME MEASURES: Measurement at coronary plane: [1]orbit-rotundum distance (distance from the lowest point of inferior orbotal fissure to the center of rotundum); ② rotundum- pterygoid canal distance Ⅰ (vertical distance from the center of rotundum to the center of pterygoid canal); ③ rotundum- pterygoid canal distance Ⅱ (Distance from the area where rotundum appeared to the area where pterygoid canal appeared). Measurement at horizontal plane: [1]anterior-posterior dimension ( the largest distance between anterior and posterior walls which paralleled to perpendicular plate of palatine bone in each layer); ② lateral dimension (distance from midpoint of perpendicular plate of palatine bone to midpoint of line, which was between outermost sphenoidal process in the anterior wall of pterygopalatine fossa and foremost evagination in the posterior wall of pterygopalatine fossa). Morphology of pterygopalatine fossa in each layer was observed at coronary and horizontal planes, separately.RESULTS: ①At the coronary plane, the 1st to 6th layers of bilateral pterygopalatine fossa presented with inclined inverted trapezoid (70%, 21 sides), and inclined quadrilateral (30%, 9 sides), and the 7th to 10th layers of bilateral pterygopalatine fossa presented with canal-shape (100%). Themean value of right orbit-rotundum distance was (5.0±2.7) mm, andthat of left orbit-rotundum distance was (5.3±2.1) mm; The mean value of right rotundum- pterygoid canal distance Ⅰ was (6.4±3.9) mm, and that of left rotundum- pterygoid canal distance Ⅰ was (6.1±4.3) mm; The mean value of right rotundum- pterygoid canal distance Ⅱ was (7.3±2.6) mm, and that of left rotundum- pterygoid canal distance Ⅱ was (7.5±2.1) mm. ② At horizontal plane: The anterior and posterior walls of the 1st and 2nd layers of bilateral pterygopalatine fossa mainly presented with double curves with palinal convex surfaces (80%, 24 sides); the 3rd and 4th layers mainly presented with opposing convex surfaces (66.7%, 20 sides); The 5th to 6th layers mainly presented with canal shape (60%, 18 sides);And the 7th to 10th layers presented with canal shape (100%). At horizontal plane, for the pterygopalatine fossa in the 1st to 6th layers, its lateral dimension was larger than its anteroposterior dimension, and for the pterygopalatine fossa in the 7th to 10th layers, its lateral dimension was equal to its anteroposterior dimension. There were no significant differences of aperture in each layer of pterygopalatine fossa between bilateral samples (P＞ 0.05).CONCLUSION: Anatomical studies on the section of osseous pterygopalatine fossa retrieve the limitation in local anatomy,and provide reliable anatomical evidence for imageology of pterygopalatine fossa and related surgical operation.

OBJECTIVE To improve efficiency and quality of antibiotic drug usage in order to assure patients′ security of antibiotic drug usage. METHODS Inducted by strengthening security quality of medical treatment,we are bring antibiotic drug usage into medical quality management by using information technique,training medical workers,putting antibiotic drug into different classifications and surveillancing the usage of antibiotic drug.Moreover,we develop significant basic study for clinic to improve the level of rational use of drug. RESULTS Frequency and number of days for drug usage were reduced by putting measures into effect and inspecting numbers and sorts of drug usage.At the same time,we instituted individualized drug using scheme to critical patients,thereby many critical patients were retrieved. CONCLUSIONS Rational use of drug is a system project which must redeploy all enthusiasm and take comprehensive measures to fight for a safe,effective,economical goal in our work.

Objective:To investigate the possibility for human papillomavirus (HPV)infection to be a predictable signal for the carcinogenesis of oral mucosa by comparing the prevalences of HPV in each stage of oral mucosal carcinogenesis and to compare the sensitivity differences of the two methods in de-tecting HPV infection in oral cavity.Methods:The hybrid capture (HC-Ⅱ)was used to detect infection of HPV in 255 samples taken from 1 2 cases of healthy oral mucosa,21 1 cases of patients with pathologi-cal diagnosis and 32 cases of patients with clinical diagnosis.The diagnosed cases included 8 cases of be-nign lesions of the oral mucosa,precancerous lesions [74 cases of oral leukoplakia (OLK)with hyper-plasia and 42 cases of OLK with oral epithelial dysplasia (OED)],91 cases of precancerous condition
[oral lichen planus (OLP)]and 28 cases of oral squamous cell carcinoma (OSCC).And in situ hybri-dization (ISH)was used to detect infection of HPV in 33 cases of OSCC and 76 cases of OLK,including 30 cases of hyperplasia,1 5 cases of mild OED,1 5 cases of moderate OED and 1 6 cases of severe OED. Results:The prevalence of HPV in OLP samples was higher (1 2.1 2%,8/66 )than that of OLK (2.59%,3/1 1 6)(χ2 =4.666,P=0.031 )and OSCC(7.1 4%,2/28,χ2 =0.51 3,P=0.474).The prevalence of HPV in OSCC (7.1 4%,2/28)was higher than that of OLK (2.59%,3/1 1 6),and no significant difference was found.There was only one case of smoke spot and statistical analysis was not carried out.ISH was used to detect type 1 6/1 8 and type 31 /33 HPV DNA in 1 09 cases of oral mucosal lesions in paraffin sections and only one case of OSCC was HPV positive.Thirty-seven cases were detec-ted by HC-Ⅱ and ISH methods at the same time.The same negative results by the two methods were found in 94.6% samples (35/37).In the other two samples,one was OSCC with early infiltration and the other was OLK with hyperplasia,The HC-Ⅱ results were positive while the ISH results were nega-tive.The patients with OLP and HPV testing results were followed up and the average follow-up period was (36.2 ±1 0.5)months.It was found that three of them had a malignant transformation,and the ma-lignant transformation rate of HPV positive patients was 1 2.50% (1 /8),which was higher than that of HPV negative patients (3.45%,2/58),and the difference was not statistically significant,P=0.249. Conclusion:HC-Ⅱ assay was more sensitive in detecting HPV infection of oral mucosal lesions than ISH.The results of this study showed that there was insufficient evidence for taking HPV infection as a predictor of OLK carcinogenesis.Patients suffering from OLP were in a precancerous condition.The pre-valence of HPV in OLP patients of this study was higher than that in OLK and OSCC patients,suggesting that for some reason,OLP patients were susceptible to HPV.HPV testing can be considered as routine in patients with OLP,and HC-Ⅱassay was recommended.And patients with OLP and HPV positive should be followed up regularly.

[ABSTRACT]AIM:ToexploretheeffectofretinoidXreceptor(RXR)agonistbexarotene(Bex)andvitaminD receptor (VDR) agonist calcitriol (Cal) on the expression of nuclear factor-kappa B (NF-κB) and the development of atherosclerosis in streptozotocin-induced diabetic apolipoprotein E knockout ( STZ-ApoE-/-) mice.METHODS: Male mice were treated for 12 weeks as follows:(1) C57+vehicle;(2) ApoE-/-+vehicle;(3) STZ-ApoE-/-+vehicle;(4) STZ-ApoE-/-+Bex (10 mg· kg-1· d-1);(5) STZ-ApoE-/-+Cal (10 μg/kg, twice a week);(6) STZ-ApoE-/-+Bex (10 mg· kg-1· d-1) +Cal (10 μg/kg, twice a week).Intraperitoneal injection of STZ was performed to establish the diabetic animal model .Western blotting and immunohistochemical method was used to detect NF-κB level in the thorac-ic aorta.Plaque area in the thoracic aorta was measured using HE staining .RESULTS:Compared with the C57 mice, the fasting blood glucose in the ApoE-/-mice was not remarkably changed .The levels of total cholesterol ( TC) and low-densi-ty lipoprotein ( LDL) were greatly increased .The fasting blood glucose and lipid levels in STZ-ApoE-/-group were much higher than those in ApoE-/-group.Compared with STZ-ApoE-/-group, the fasting blood glucose and lipid levels in Bex group and Cal group were not significantly changed .Compared with the C57 mice, the protein expression of NF-κB in the ApoE-/-mice and the STZ-ApoE-/-mice was remarkably increased .Compared with STZ-ApoE-/-group, the levels of NF-κB in Bex group, Cal group and combination group were greatly decreased .Compared with STZ-ApoE-/-group, the thoracic artery plaque areas in Bex group and Cal group were inhibited (both P<0.05).Compared with Bex group, the plaque area of the thoracic artery in combination group was significantly decreased (P<0.05).CONCLUSION:Bexaro-tene or calcitriol decreases the development of atherosclerosis in streptozotocin -induced diabetic ApoE-/-mice.Bexarotene combined with calcitriol affords greater protection than monotherapy .The mechanism may be involved in down-regulating the expression of NF-κB.

Objective To evaluate CT characteristics of pulmonary sclerosing hemangioma(PSH) so that to improve the knowledgeof this disease.Methods CT characteristics of PSH pathologically verified were retrospectively analysed.Conventional scan andcontrast-enhanced scan were performed in all the patients.Results 14 lesions were in right lung,while 10 were in left,Diameters of these lesions ranged from 0.8 to 10 cm,and the average diameter was 3.08 cm.3 lesions were located in hilum of lung,while the rest(87.5%)were located in peripheral lung tissue.20 lesions were oval,and 4 were lobulated in morphology.21 lesions were smooth in edge.45.8% of the cases had calcification.All the lesions enhanced obviously on contrast-enhanced scan,and average CT value of(46.62?9.47) HU was increased.The dilated and distorted blood vessels could be detected in 2 large lesions.Conclusion On the basis of general benign tumor features, obvious enhancement and calcification are the characteristics of PSH on CT.

Objective To construct the recombinant adenovirus of mouse Osf2/Cbfal gene and to observe its ability to infect NIH3T3 fibroblasts. Methods The Osf2/Cbfal gene fragment was cloned into the shuttle plasmid pAdTrack-CMV to form the transfer vector by the method of homogenous recombination in bacteria. Then the recombinant adenovirus was transfected into NIH3T3 cells using Lipofectine DOTAP, The target gene was detected by poly-merase chain reaction (PCR). The titer and its infection rate were determined using the green fluorescent protein (GFP) expression in the shuttle plasmid. Results Restriction endonuclease and PCR analyses confirmed that the Osf2/Cbfal gene was successfully inserted into the adenovirus vector. The titer of the recombinant adenovirus was 1.6?1012 pfu/ml. The adenovirus had a strong effect on NIH3T3 cells. Conclusion The recombinant adenovirus containing Osf2/Cbfal gene was successfully constructed by the method of homogenous recombination in bacteria.