OBJECTIVE To assess the association of SLC12A3 and SCNN1B gene polymorphisms (rs11643718 and rs12447134) with essential hypertension among ethnic Koreans from Mudanjiang, China. METHODS For 204 patients with essential hypertension and 186 healthy controls, the genotypes of rs11643718 and rs12447134 loci were determined with an improved multiplex ligase detection reaction (iMLDR) method. RESULTS Allelic and genotypic frequencies of rs11643718 of SLC12A3 gene are associated with the onset of disease hypertension (P <0.05) as well as systolic blood pressure (P < 0.01, under a recessive model). No association was found between rs12447134 of SCNN1B gene with the onset of disease (P > 0.05) but diastolic blood pressure (P < 0.05, under a recessive model). CONCLUSION The polymorphisms of rs11643718 locus is associated with the susceptibility for essential hypertension among ethnic Koreans from Mudanjiang area and can be used as a predictor for the disease.

Objective To observe the effect of acanthopanax refined polysaccharide(ASPS)on nicotine-induced learning and memory impairment in mice.Methods A total of 48 male mice and 48 females at 6 weeks of age were selected and were divided into two batches for animal experiments:the Morris and the new object recognition batch.Each batch was randomly divided into 6 groups ac-cording to body weight:blank control group,model group,drug positive group,high-dose ASPS group,medium-dose ASPS group,and low-dose ASPS group.Except for the blank group,the remai-ning 5 groups were injected subcutaneously with 0.5mg/kg of nicotine every day for 7 days to prepare a nicotine memory disorder model.After 24 hours of injection of nicotine at the 7th day,the drug posi-tive group was gavaged piracetam for 800 mg/kg,and the high-dose,medium-dose and low-dose ASPS groups were gavaged for 270,90 and 30 mg/kg of ASPS respectively for 7 days.The learning and memory ability of mice was detected by water maze test and new object recognition test,respec-tively.After the two tests,superoxide dismutase(SOD)activity in serum and 5-Hydroxytryptamine(5-HT)content in hippocampal tissue were detected.Results The results of new object recognition experiment showed that the discrimination indexes of the high-,medium-and low-dose ASPS groups were significantly higher than that of the model group(P＜0.01 or P＜0.05).The results of water maze experiment showed that the time to find the platform in the spatial search experiment was signifi-cantly shorter in the high-and medium-dose ASPS groups than that in the model group(P＜0.05).In the positioning voyage test,the number of mouse platform entries in the high-dose ASPS group was significantly more than that in the model group(P＜0.05);the proportions of Ⅲ quadrant routes in the high-and medium-dose ASPS groups were higher than those in the model group(P＜0.05).The high-,medium-and low-dose ASPS groups were significantly higher than those in the model group(P＜0.01);the determination of hippocampal tissue content in mice showed that the content of 5-HT in the high-and medium-dose ASPS groups was significantly higher than that in the model group(P＜0.01 or P＜0.05).Conclusion ASPS can significantly improve the learning and memory ability of nicotine-quitting mice,relieve the damage of hippocampal neurotransmitters,and regulate oxidative stress in vivo.The mechanism may be related to improving the body's antioxidant capacity and regulating hippocampal neurotransmitter levels.

Objective To observe the effect of acanthopanax refined polysaccharide(ASPS)on nicotine-induced learning and memory impairment in mice.Methods A total of 48 male mice and 48 females at 6 weeks of age were selected and were divided into two batches for animal experiments:the Morris and the new object recognition batch.Each batch was randomly divided into 6 groups ac-cording to body weight:blank control group,model group,drug positive group,high-dose ASPS group,medium-dose ASPS group,and low-dose ASPS group.Except for the blank group,the remai-ning 5 groups were injected subcutaneously with 0.5mg/kg of nicotine every day for 7 days to prepare a nicotine memory disorder model.After 24 hours of injection of nicotine at the 7th day,the drug posi-tive group was gavaged piracetam for 800 mg/kg,and the high-dose,medium-dose and low-dose ASPS groups were gavaged for 270,90 and 30 mg/kg of ASPS respectively for 7 days.The learning and memory ability of mice was detected by water maze test and new object recognition test,respec-tively.After the two tests,superoxide dismutase(SOD)activity in serum and 5-Hydroxytryptamine(5-HT)content in hippocampal tissue were detected.Results The results of new object recognition experiment showed that the discrimination indexes of the high-,medium-and low-dose ASPS groups were significantly higher than that of the model group(P＜0.01 or P＜0.05).The results of water maze experiment showed that the time to find the platform in the spatial search experiment was signifi-cantly shorter in the high-and medium-dose ASPS groups than that in the model group(P＜0.05).In the positioning voyage test,the number of mouse platform entries in the high-dose ASPS group was significantly more than that in the model group(P＜0.05);the proportions of Ⅲ quadrant routes in the high-and medium-dose ASPS groups were higher than those in the model group(P＜0.05).The high-,medium-and low-dose ASPS groups were significantly higher than those in the model group(P＜0.01);the determination of hippocampal tissue content in mice showed that the content of 5-HT in the high-and medium-dose ASPS groups was significantly higher than that in the model group(P＜0.01 or P＜0.05).Conclusion ASPS can significantly improve the learning and memory ability of nicotine-quitting mice,relieve the damage of hippocampal neurotransmitters,and regulate oxidative stress in vivo.The mechanism may be related to improving the body's antioxidant capacity and regulating hippocampal neurotransmitter levels.