In this study, we conducted test-linked lectures (using a pre-test and a poster related to the pre-test) to understand the contents of “genetic testing-related knowledge” for university festival visitors. We also assessed their level of understanding and the effectiveness of the lectures pre- and post-test, by dividing the visitors into groups according to gender, medication, and age. First, pharmacy students performed a pre-test (9 questions, each with 4 answer choices) on “flow of genetic information, protein structure and function, relation between gene and protein/living body, basic knowledge of genetic testing and sampling method” for the visitors. Second, lectures were conducted using posters that included the contents of the pre-test. Finally, a post-test was performed with the same content as that of the pre-test. The results revealed a high correct-response rate (more than 65%) for the post-test in most groups (except for some groups with visitors in their 70s). In addition, compared to the overall pre-test scores, those of the post-test showed a significant increase. Thus, we elucidated that test-linked lectures contribute to the comprehension degree improvement in visitors aged from their teens to their 60s ; the validity of lectures using pre-test and poster was also proved.