1.Hypoxia upregulates glucose-6-phophate isomerase expression and improves proliferation of fibroblast-like synoviocytes from synovium of patients with rheumatoid arthritis
Ying LU ; Ming ZONG ; Shasha FAN ; Tianbao LU ; Xingmiao DAI ; Lieying FAN
Chinese Journal of Rheumatology 2015;19(5):327-331,后插1
Objective This study was performed to investigate the effect of hypoxia on glucose-6-phosphate isomerase (G6PI) expression and cell cycle of fibroblast-like synoviocytes from synovium of rheumatoid arthritis (RA) and osteoarthritis (OA) under hypoxia or normoxia.Methods Fibroblast-like synoviocytes were cultured with either of hypoxia (3% oxygen) or normoxia (21% oxygen) for 24 hours.The mRNA expression of G6PI and HIF-1α was tested by PCR quantification,while the protein levels of G6PI and HIF-1α were measured by western blot.Cell cycle was performed by FACS.T-test and Mann-Whitney U were used for statistical analysis.Results The expression levels of G6PI mRNA under hypoxia in RA were higher than those of OA (2.6±0.4 vs 1.5±0.4,P<0.05).The protein levels of G6PI in RA were higher than those of OA (P<0.05).The expression levels of HIF-1α mRNA under hypoxia in RA were higher than those of OA (2.9±0.8vs 1.4 ±0.4,P<0.05).The protein levels of HIF-1α in RA were higher than those of OA (P<0.05).The G1 phase ratio of cell cycle was decreased significantly under hypoxia than those of normoxia in RA ELs (t=1 1.31,P<0.05).The S and G2 phase ratio of cell cycle were increased.Conclusion Hypoxia upregulates G6PI and HIF-1α expression and improves proliferation in fibroblast-like synoviocytes.
2.Bone marrow stem cells for treatment of liver cirrhosis.
Ying HAN ; Kai-chun WU ; Dai-ming FAN
Chinese Journal of Hepatology 2009;17(4):249-251
3.Expression of IL-6 in kainic acid-induced seizure and regulation roles of nitric oxide pathway
Yiping SUN ; Changkai SUN ; Ming FAN ; Jie ZHAO ; Dayue HAN ; Jiqing WANG ; Dezheng GONG ; Shufang DAI ; Hong XU ;
Chinese Pharmacological Bulletin 1986;0(04):-
AIM To explore the role of nitric oxide (NO) mediators in seizure behavior and the related expression of interlukin 6(IL 6). METHODS The IL 6 immunoreactivity (IL 6 ir) and behaviour were observed in kainic acid (KA) induced seizure following pretreatment with L nitroarginine( L NNA), a inhibitor of nitric oxide synthase(NOS), or the same number of moles of L arginine( L Arg). RESULTS The results showed that time dependent seizures were induced on animals after administration of KA (10 mg?kg -1 ), accompanied by the immediate enhancement of IL 6 ir in hippocampal structure, piriform area and cortex. The behaviors of rats were not markedly altered by chronic pretreatment with L NNA (50 mg?kg -1 ) or L Arg (40 mg?kg -1 ). However, after the administration of KA, the seizure behaviors were obviously different in the group of L NNA and L Arg respectively. Seizure was agrevated in the animals with L NNA pretreatment and many rats died at KA 3 hours, whereas seizure was alleviated after KA in the group of L Arg. IL 6 expression was apparently up regulated in some brain areas such as hippocampus in the group of KA pretreated with L NNA, but opposite effects appeared in the group pretreated with L Arg before KA injection. CONCLUSION These results indicate that endogenous NO mediators may alleviate the seizure behaviors and may down regulated the early expression of IL 6 in KA challenged seizure, but it the mechanism of the early expression of IL 6 with the homeostasis of endogenous NO mediator merits further study.nismoftheearlyexpressionofIL 6withthehome
4.A study on the relationship between portal vein pressure and portal hemodynamics in patients with portal hypertension of cirrhosis.
Ming-tao LIU ; Guo-hong HAN ; Jian-hong WANG ; Kai-chun WU ; Dai-ming FAN
Chinese Journal of Hepatology 2004;12(8):504-505
Adult
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Aged
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Female
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Hemodynamics
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Hepatitis B, Chronic
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complications
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Humans
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Hypertension, Portal
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diagnostic imaging
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physiopathology
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Liver Cirrhosis
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diagnostic imaging
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physiopathology
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Male
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Middle Aged
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Portal Vein
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diagnostic imaging
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physiopathology
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Ultrasonography, Doppler, Color
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Venous Pressure
5.Chemical constituents from the n-butanol fraction of Polygonum amplexicaule var.sinense
Fan ZHAO ; Zhe-Ming WANG ; Bi-Qing ZHAO ; Qun-Gang QIAN ; Li LI ; Wan DAI ; Guang-Ming XU ; Xiao-Jiang ZHOU
Chinese Traditional Patent Medicine 2018;40(3):626-629
AIM To study the chemical constituents from the Polygonum amplexicaule var.sinense.METHODS The n-butanol fraction of ethanol extract from P.amplexicaule var.sinense was isolated and purified by TLC,normal-phase silica,macroporous resin column,sephadex column,preparative TLC and semi-preparative HPLC column,then the structures of obtained compounds were identified by physicochemical properties and spectral data.RESULTS Ten compounds were identified as 2-(aminomethyl)-4-methoxy-phenol (1),p-methyl-hydroxybenzoate (2),p-methyl-hydroxyphenylacetate (3),2-[2-(methylamino) phenyl]-4-thiazolecarboxylic acid,methyl ester (4),p-methylphenylethanol (5),bergenin (6),arbutin (7),rhododendron-2-O-β-D-glucopyranoside (8),n-butyl gallate (9) and β-sitosterol (10).CONCLUSION Compounds 1-8 are isolated from this plant for the first time.
6.Blood lead status and influencing factors among preschool children in urban areas of China.
Zang-Wen TAN ; Yao-Hua DAI ; Xiao-Hua XIE ; Shuai-Ming ZHANG ; Zhao-Yang FAN ; Ni JIA
Chinese Journal of Pediatrics 2011;49(4):294-300
OBJECTIVETo investigate the blood lead status and influencing factors among preschool children in the sampling city.
METHODStratified-clustered-random sampling was used. Standardized questionnaire and peripheral blood samples were obtained from 69 968 children aged 0-6 years in fixed kindergartens and communities of Yinchuan, Xi'an, Chengdu, Wuhan, Hefei, Beijing, Harbin, Zhengzhou, Huhhot, Shijiazhuang, Haikou, Dalian, Qingdao, Guangzhou, Nanning and Changsha from 2004 to 2008, respectively. Tungsten atomic absorbtion spectrophotometry was employed to determine the blood lead level of children.
RESULTThe proportion of children with blood lead level ≥ 100 µg/L was 7.57% (among which the proportion of high blood lead level, mild lead poisoning, moderate lead poisoning, severe lead poisoning were 91.0%, 2.76%, 3.32%, 2.93%, respectively) and the blood lead level was lower than those of the past studies. The proportion of high blood lead level has steadily declined from 2004 to 2008 [the proportions were 10.03%, 7.85%, 7.40%, 6.91% and 4.78%, respectively (χ(2) = 297.36, P < 0.0001)]. The proportion of children with blood lead level ≥ 100 µg/L in Haikou, Zhengzhou, Guangzhou, Shijiazhuang, Changsha, Xi'an, Wuhan, Hefei, Chengdu, Yinchuan, Harbin, Beijing, Dalian, Huhhot, Nanning and Qingdao were 12.15%, 10.49%, 10.37%, 9.69%, 9.53%, 9.46%, 9.40%, 8.50%, 7.99%, 7.98%, 7.51%, 6.10%, 3.25%, 2.89%, 2.46% and 2.39%, respectively (χ(2) = 768.21, P < 0.0001). By multiple regression method, the risk factors which influenced blood lead status of children were education status of mother, older children, behavior and dietary habit of children, boy, stay for long time in traffic busy areas, the type of housing, taking traditional Chinese and herbal medicine. The protective factors against lead poisoning in children mainly included scattered living, the nutritional status of calcium, iron, zinc, frequent intake of milk, and older mother.
CONCLUSIONThe blood lead level of children has decreased, but is still higher than those in developed countries. Lead exposure remains a public health issue which affects children most. The blood lead level of children is affected by multiple factors. Government and the whole society should pay attention to interrupt the lead pollutant and to promote nutritional health education. With all these efforts, it is possible to stop the progress of lead exposure and reduce its hazardous effects on the growth and development of children.
Child ; Child, Preschool ; China ; epidemiology ; Female ; Humans ; Infant ; Lead ; blood ; Lead Poisoning ; epidemiology ; Male ; Risk Factors ; Surveys and Questionnaires ; Urban Population
7.Multidrug resistant effect of alternative splicing form of MAD2 gene-MAD2beta on human gastric cancer cell.
Fang YIN ; Wen-hua HU ; Tai-dong QIAO ; Dai-ming FAN
Chinese Journal of Oncology 2004;26(4):201-204
OBJECTIVETo study the effect of alternative splicing form -MAD2beta of mitotic arrest deficient protein 2 (MAD2) on the formation of multidrug resistance in human gastric adenocarcinoma cell SGC7901.
METHODSRNA was extracted from a multidrug resistance cell line SGC7901/ADR. The full-length MAD2beta cDNA was obtained by RT-PCR and cloned into the pUCm-T vector, and then recombined into the eukaryotic expression vector pcDNA3.1 in forward direction. Subsequently, pcDNA3.1/MAD2beta vectors were then transfected into SGC7901 cells by lipofectamine. Sensitivity to drug was detected by MTT assay. Cell cycle alteration and intracellular fluorescence intensity were determined by FACS.
RESULTSA fragment of 0.53 Kb was obtained and confirmed by DNA sequencing which was a new alternative splicing form of MAD2 named as MAD2beta. pcDNA3.1/MAD2beta transfected SGC7901 cells (SGC7901/MAD2beta) were more resistant to ADR, VCR and MMC than the control cells (SGC7901/pcDNA3.1), and also ADR fluorescence intensity of SGC7901/MAD2beta cells was lower (P < 0.05) than that of SGC7901/pcDNA3.1 cells.
CONCLUSIONMAD2beta could increase the multidrug resistance of SGC7901 cell line.
Adenocarcinoma ; metabolism ; pathology ; Alternative Splicing ; Antibiotics, Antineoplastic ; pharmacology ; Antineoplastic Agents, Phytogenic ; pharmacology ; Calcium-Binding Proteins ; biosynthesis ; genetics ; Cell Cycle Proteins ; Cell Line, Tumor ; DNA-Binding Proteins ; biosynthesis ; genetics ; Doxorubicin ; pharmacology ; Drug Resistance, Multiple ; Drug Resistance, Neoplasm ; genetics ; Humans ; Mad2 Proteins ; Mitomycin ; pharmacology ; Repressor Proteins ; Smad2 Protein ; Stomach Neoplasms ; metabolism ; pathology ; Trans-Activators ; biosynthesis ; genetics ; Transfection ; Vincristine ; pharmacology
8.Meta-analysis on Helicobacter pylori infection between sex and in family assembles.
Yi WAN ; Yong-yong XU ; Fu-bo XUE ; Dai-ming FAN
Chinese Journal of Epidemiology 2003;24(1):54-57
OBJECTIVETo understand and analyze the infection situation of Helicobacter pylori (H. pylori).
METHODSExtensively reviewing Chinese literature collecting the related with electronic documents in combination with manual retrieve and using Meta-analysis to do a quantitative analysis.
RESULTSSlight difference in the infection rate of H. pylori between men and women (95% CI: 0.0579-0.0963) was noticed. The infection rate of H. pylori in children whose parent was positive with infection of this bacteria was higher than that of children whose parent was negative (95% CI: 0.3378-0.5042).
CONCLUSIONThe infection rate of H. pylori showed gender difference with nature of family aggregation. Epidemiological studies of H. pylori was comprehensive and involved many aspects. Further investigation needs to be focused on infection rate and other risk factors.
Adolescent ; Adult ; Aged ; Antibodies, Bacterial ; blood ; Child ; Child, Preschool ; China ; epidemiology ; Family Health ; Female ; Helicobacter Infections ; epidemiology ; transmission ; Helicobacter pylori ; immunology ; Humans ; Infant ; Male ; Middle Aged ; Prevalence ; Risk Factors ; Sex Factors
9.Effect of L-arginine or L-nitroarginine on kainic acid induced seizure and expression of iNOS mRNA in the rats' hippocampus
Yiping SUN ; Changkai SUN ; Ming FAN ; Jie ZHAO ; Dayue HAN ; Jiqing WANG ; Dongmei WANG ; Yan PENG ; Dezheng GONG ; Lei FU ; Shufang DAI ; Hon XU
Chinese Pharmacological Bulletin 2003;0(08):-
AIM To observe the expression of inducible nitric ox ide synthase (iNOS) in the rats' hippocampus of kainic acid (KA) induced-seizur es and the effect of L-arginine(L-Arg) or L-nitroarginine(L- NNA) chronic intervention before KA. METHODS The expression of iN OS mRNA by RT-PCR and behaviour were observed after administration of convulsan t dose of KA (10 mg?kg -1 ) and pretreatment with NO predecessor (L-Ar g, 40 mg?kg -1 ) or a inhibitor of NOS(L-NNA, 50 mg?kg -1 ) befo re KA. RESULTS The time-dependent seizures were induced on rats after giving KA, and were improved and serious in the animals with L-NNA pr etreatment, but they were alleviated by L-Arg pretreatment before KA. Compa red with control, iNOS mRNA expression was feebly at KA 3 h, continuative improv ement accompanying KA time prolonged, and it was markedly enhanced at KA 24 h. I t couldn't be examined at KA 2 d or 3 d, and was obviously improved at KA 7 d a gain. iNOS mRNA appeared weak in the rats hippocampus with L-Arg pretreatme nt after KA 1 h, whereas it wasn't found in L-NNA pretreatment animals. CONCLUSION iNOS mRNA expression appeared in the hippocampus of seizu re rats at a few time after KA, and L-Arg chronic intervention before KA ha s a little effect on it.